RAPD Analysis of DNA Polymorphism and Genetic Species-Specificity Using PCR Technique in the Marine Microalgae

  • Yoon, Jong-Man (Department of Marine Biomedical Science, Kunsan National University) ;
  • Chang, Kye-Nam (West Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
  • Published : 2000.05.01

Abstract

Genomic DNA was isolated from the marine microalgae representing genetic characteristics and genomic polymorphisms by polymerase chain reaction amplification of DNA as arbitrary primers. The electrophoretc analysis of PCR-RAPD products showed hig levels of variation between different genus and little variation between different species. Outer of these primers, 6 generated 248 highly reproducible RAPD markers, producing almost seven polymorphic bands per primers. The degree of similarity frequency between Chaetoceros gracilis and Chaetoceros calcitrans species showed 90% as calculated by sharing analysis. The RAPD polymorphism generated by this primer may be used as a genetic marker for genus or species identification in important marine microalgae. (omitted)

Keywords