Electrostatic and Hydrophobic Nature of the Cytochrome c-Membrane Interaction

  • Kim, Ukchun (Division of Biological Sciences, Kangwon National University) ;
  • Kim, Kyunghoon (Division of Biological Sciences, Kangwon National University) ;
  • Sanghwa Han (Division of Biological Sciences, Kangwon National University)
  • Published : 1999.06.01

Abstract

Cytochrome c (cyt c) binds to acidic membranes at low ionic strength. Replacement of Lys-72 or Lys-87 by Glu reduced the binding affinity of cyt c toward large unilamellar vesicles (LUV) in liquid crystalline phase. The differences were smaller for LUV in gel phase. A fraction of bound cyt c was non-electrostatically associated.(omitted)

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