Proceedings of the Korean Biophysical Society Conference (한국생물물리학회:학술대회논문집)
- 1999.06a
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- Pages.36-36
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- 1999
Crystal Structure of $\Delta$ -3-Ketosteroid Isomerase From Pseudomonas testosteroni in Complex with Equilenin Settles the Correct Hydrogen Bonding Scheme for Transition-State Stabilization
- Cho, Hyun-Soo (Department of Life Science Pohang University of Science and Technology) ;
- Ha, Nam-Chul (Department of Life Science Pohang University of Science and Technolog) ;
- Park, Gildon (Department of Life Science Pohang University of Science and Technology)
- Published : 1999.06.01
Abstract
Bacterial Δ-3-ketosteroid isomerase (KSI) catalyzes the conversion of Δ-to Δ-3-ketosteroids via enolate formation, which is also found in the synthesis of all steroid hormones in mammals. In Pseudomonas testosteroni, KSI Asp38 (pKa ~ 4.7) was identified as the general base which abstracts the steroid C4b-H (pKa ~ 12.7) to form the dienolate intermediate.(omitted)
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