Proceedings of the KOSOMBE Conference (대한의용생체공학회:학술대회논문집)
- Volume 1998 Issue 11
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- Pages.227-228
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- 1998
Evaluation of Blood compatibility at lumbrokinase immobilized polymer valves in vivo
룸브로키나제가 고정화된 폴리머 밸브의 invivo 혈액적합성 평가
- Park, Yong-Doo (Department of Biomedical Engineering, Seoul National University) ;
- Ryu, Eun-Sook (Biomedlab Co.) ;
- Kim, Jong-Won (Biomedlab Co.) ;
- Min, Byoung-Goo (Department of Biomedical Engineering, Seoul National University)
- Published : 1998.11.20
Abstract
Lumbrokinase, potent fibrinolytic enzyme purified from earthworm, was immobilized onto polyurethane valves using photoreaction, photoreactive polyallyl-amino as a photoreactive linker. For evaluation of blood compatibility, lumbrokinase immobilized polymer valves were assembled into the total artificial heart (TAH). This TAH was implanted to 60kg healthy lamb for 1-3 days with the cardiac output 5 L/min. In the control lamb, the valves were untreated, in ore other, only valves on the right were treated, and in the remaining animal, only those on the left. To facilitate the thrombus formation, low doses of heparin were administered. For evaluation of the immobilized lumbrokinase, thrombus formation, proteolytic and fibrinolytic activity was measured. This data shows that lumbrokinase-treated polyurethane valves lead to decreased thrombus formation in vivo, and that their biocompatibility is therefore higher than that of untreated valves.