한국응용약물학회:학술대회논문집 (Proceedings of the Korean Society of Applied Pharmacology)
- 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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- Pages.183-183
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- 1998
Evaluation of Telomerase Inhibitors Using DE81 Filter Spotting Method from Natural Products
- Lee, Sung-Jin (Natural Products Research Institute, Seoul National University) ;
- Woongchon Mar (Natural Products Research Institute, Seoul National University)
- 발행 : 1998.11.01
초록
Telomerase synthesizes telomeric DNA repeats onto chromosome ends de novo. Telomerase activation and telomere shortening in human somatic cells have been implicated in cell tumorigenesis and immortalization. In order to find the potential inhibitors against telomerase activitiy which can be used as potential anticancer agents, we screened about 100 kinds of natural products after partition into n-hexane, ethyl acetate and aqueous layers from methanol extracts. The inhibitory effects of these materials against telomerase enzyme activity were tested in 293T cell culture using telomeric repeat amplification protocol(TRAP). The incorporation of
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