한국생물물리학회:학술대회논문집 (Proceedings of the Korean Biophysical Society Conference)
- 한국생물물리학회 1996년도 정기총회 및 학술발표회
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- Pages.6-6
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- 1996
STUDIES OF CELL COMMUNICATION BY USING GAP JUNCTION CHANNELS RECONSTITUTE IN UNILAMELLAR LIPID VESICLES
초록
Gap junction channels were reconstituted into unilamellar liposomes using immunoaffinity purified connexin 32 gap junction protein from rat liver. Vesicles containing open channels and close channels were separated by means of iso-osmolar sucros density gradient sedimentation. The open channels formed in lipid vesicles were permeable to a fluorescent dye molecule, lucifer yellow of which the hydrodynamic size is similar to pore size of gap junctions in vivo. (omitted)
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