한국응용약물학회:학술대회논문집 (Proceedings of the Korean Society of Applied Pharmacology)
- 한국응용약물학회 1996년도 춘계학술대회
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- Pages.281-281
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- 1996
Novel Liposome Immunoassay for Detection of Ultratrace Amount of Bioactive Substances : an Assay for Insulin
- Lim, Soo-Jeong (Department of Physical Pharmacy, College of Pharmacy, Seoul National University) ;
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Kim, Chong-Kook
(Department of Physical Pharmacy, College of Pharmacy, Seoul National University)
- 발행 : 1996.04.01
초록
The immunoassay method is frequently used for the identification and quantitation of ultratrace amount of bioactive substances. Homogeneous liposome immunoassays, which can avoid the use of radioisotopes and separation steps, have recently been reported in many publications. Cytolysin-mediated liposome immunoassay using melittin ever been studied but showed limited applications. Here, we designed a homogeneous liposome immunoassay using Clostridium perfringens phospholipase C (PLC), an enzyme which catalyzes the hydrolysis of phosphatidylcholine in biological membranes, as a cytolysin.
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