한국미생물학회:학술대회논문집 (Proceedings of the Microbiological Society of Korea Conference)
- 한국미생물학회 1991년도 춘계학술발표대회 논문집
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- Pages.237-242
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- 1991
MOLECULAR BREEDING OF GLUTATHIONE PRODUCING BACTERIAL STRAINS
- 남용석 ;
- Nam Yong-Suk (Department of Argricultural Chemistry, Korea University) ;
- Lee Se Yong (Department of Argricultural Chemistry, Korea University)
- 발행 : 1991.04.01
초록
In order to increase the production of glutathione by maximizing the expression of recombinant gsh plasmids, two genes responsible for the biosynthesis of glutathione were cloned. A gshI gene was cloned onto pBR322 plasmid as 3.6Kb PstI DNA fragment from E. coli K-12 chromosomal DNA. Also gshII gene was cloned onto pUC13 plasmid as 2.2Kb PstI-BamHI DNA fragment. In order to improve the glutathione producing activity more efficiently, various recombinant plasmids containing tandem repeated gshI genes or both genes in various copy number onto the same vector were constructed. E. coli cells harboring pGH501 plasmid (pUC8-gshI
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