Proceedings of the Korean Society for Applied Microbiology Conference (한국미생물생명공학회:학술대회논문집)
- 1986.12a
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- Pages.525.3-526
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- 1986
Fermentation of carboxymethylcellulase using recombinant DNA-Bacillus megaterium
- Son, Kwang-Hee (Korea Advanced Institute of Science & Technology) ;
- Jang, Jong-Hyun (Korea Advanced Institute of Science & Technology) ;
- Kim, Jung-Hoe (Korea Advanced Institute of Science & Technology)
- Published : 1986.12.01
Abstract
For the analysis of fermentation characteristics and productivity of plasmid coded product, car-boxymethylcellulase in a recombinant DNA cell fermentation system, batch and continuous fermentations were carried out using a Bacillus megaterium ATCC 14945 transformed with a plasmid, pCK 108 haboring carboxymethyl cellulase gene. The effects of carbon and nitrogen sources and of temperature and pH on cell growth, product yield, plasmid stability, specific plasmid contents of cell, and gene expression efficiency were carefully studied. These experimental results will be discussed in some details.
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