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Cellmed Orthocellular Medicine and Pharmaceutical Association (셀메드 세포교정의약학회)
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Neurobehavioural effects of exposure of wistar rats to smoke from traditional Carica papaya (pawpaw) leaves

  • Received : 2012.09.07
  • Accepted : 2012.11.21
  • Published : 2012.11.30
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Abstract

This study was carried out to investigate the neurobehavioural effects of the smoking of Carica papaya leaves based on ethnopharmacological information. Twenty -four rats used for the study were grouped into four groups namely; Groups 1 (Control, unexposed to any smoke) and Groups 2 - 4 (exposed to smoke from varying amount of pawpaw leaf powder). Groups 2, 3, and 4 were exposed to smoke from 6.25 g (low dose), 12.50 g (medium dose), and 18.75 g (high dose) of dry pawpaw leaves respectively in a smoking chamber twice daily for 21 one days with each exposure lasting 3 min. The spontaneous motor activities and locomotory behaviour of the animals were assessed using an open field maze and hole board maze test. The results showed that the smoking of Carica papaya leaves led to a significant (p < 0.05) increase in locomotory activities from $33.36{\pm}3.36$ (control) to $66.50{\pm}6.16$ (high dose), number of head dips (from $8.50{\pm}0.36$, to $12.83{\pm}0.87$) and rearing behaviour (from $14.83{\pm}0.74$, to $27.0{\pm}1.46$). However, the freezing durations as animals were introduced into the maze and grooming behaviour were significantly (p < 0.05) reduced in the treated groups. In conclusion, the increased ambulations and exploratory behaviours imply that the anxiety level is low in the treated groups compared to the control group which thus confirms that dry Carica papaya leaf may possibly contain psychoactive substance(s) that become active when smoked.

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References

  1. Bhattacharya SK, Clow A, Przyborowska A, Halket J, Glover V, Sandler M. Effect of aromatic amino acids, pentylentetrazole and yohimbine on isatin and tribulin activity in rat brain. Neurosci Lett. 1991;132:44-46. https://doi.org/10.1016/0304-3940(91)90429-W
  2. Bhattacharya SK, Satyan KS. Experimental methods for evaluation of psychotropic agents in rodents: I-antianxiety agents. Indian J Exp Biol. 1997;35:565-575.
  3. Briley M, Chopin P, Moret C. Effect of serotonergic lesion on "anxious" behaviour measured in the elevated plus maze test in the rat. Psychopharmacol (Berl). 1990;101:187-189. https://doi.org/10.1007/BF02244124
  4. Burdick EM. Carpaine: An alkaloid of Carica papaya - Its chemistry and pharmacology. Econ. Bot. 1971;25:363-365. https://doi.org/10.1007/BF02985202
  5. Carlini EA. Plants and the central nervous system. Pharmacol, Biochem Behav. 2003;75:501-512. https://doi.org/10.1016/S0091-3057(03)00112-6
  6. Carpenedo R, Chiarugi A, Russi P, Lombardi G, Carlà V, Pellicciari, R, Mattoli L, Moroni F. Inhibitors of Kynurenine hydroxylate and Kynureniase increase cerebral formation of Kynueniase and have sedative and anti-convulsant activities. Neurosci. 1994;61:237-243. https://doi.org/10.1016/0306-4522(94)90227-5
  7. Chavez-Quintal P, Gonzalez-Flores T, Rodriguez-Buenfil I, Gallegos-Tintore S. Antifungal Activity in Ethanolic Extracts of Carica papaya L. cv. Maradol Leaves and Seeds. Indian J Microbiol. 2011;51:54-60. https://doi.org/10.1007/s12088-011-0086-5
  8. Dallwitz MJ. A general system for coding taxonomic descriptions. Taxon.1980;29:41-46. http://delta-intkey.com/www/dallwitz-1980.pdf https://doi.org/10.2307/1219595
  9. Dhawan K, Kumar S, Sharma A. Anti anxiety studies on extracts of Passiflora incernata Linneaus. J Ethnopharmacol. 2001;8:165-170.
  10. Ferrari F, Pelloni F, Giuliani D. Suppressive effect of the dopamine D2 receptor against B-HT 920 on rat grooming. Eur J P harmacol.1992;216:345-350.
  11. File SE, Wardill AG. Validity of head-dipping as a measure of exploration in a modified hole board. Psychopharmacol. 1975;44:53-59. https://doi.org/10.1007/BF00421184
  12. Gill LS. Ethnomedical Uses of Plants in Nigeria. (Benin City, Nigeria: Uniben Press), 1992. http://openlibrary.org/books/OL742829M/Ethnomedical_uses_of_plants_in_Nigeria
  13. Henningfield JE, Miyasato K, Jasinski DR. Abuse liability and pharmacodynamic characteristics of intravenous and inhaled nicotine. J Pharmacol Exp Ther. 1985;234:1-12.
  14. Holmes A, Wrenn CC, Harris AP, Thayer KE, Crawley JN. Behavioural profiles of inbred strains on novel olfactory, spatial and emotional tests for reference memory in mice. Genes Brain Behav. 2002;1:55-69. https://doi.org/10.1046/j.1601-1848.2001.00005.x
  15. Hsieh M, Wu C, Chen C. Gastrodin and p-hydroxybenzyl alcohol facilitate memory consolidation and retrieval, but not acquisition, on the passive avoidance task in rats. J Ethnophamacol. 1997;56:45-54. https://doi.org/10.1016/S0378-8741(96)01501-2
  16. Joshi BS, Newton MG, Lee DW, Barber AD, Pelletier SW. Reversal of absolute stereochemistry of the pyrrolo[2,1-b]quinazoline alkaloids vasicine, vasicinone, vasicinol and vasicinolone. Tetrahedron Asym. 1996;7:25-28. https://doi.org/10.1016/0957-4166(95)00412-2
  17. Maniyar Y, Bhixavatimath P. Antihyperglycemic and hypolipidemic activities of aqueous extract of Carica papaya Linn. leaves in alloxan-induced diabetic rats. J Ayurveda Integr Med. 2012;2:70-74.
  18. Melariri P, Campbell W, Etusim P, Smith P. Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves. J Parasitol Res. 2011;10:49-54.
  19. Mesembe OE, Bisong SA, Ekong MB, Ekeoma AO. Neurobehavioural activity in albino Wistar rats in the open field maze following long term tobacco diet ingestion. The Internet J Neurol. 2009;10:13.
  20. Monti R, Contiero J, Goulart AJ. Isolation of natural inhibitors of papain obtained from Carica papaya latex. Braz Arch Biol Technol. 2004;47:757-754.
  21. Nawrot P, Jordan S, Eastwood J, Rotstein J, Hugenholtz A, Feeley M. Effects of caffeine on human health. Food addit Contam. 2003;20:1-30.
  22. Novas ML, Wolfman C, Medina JH, Robertis E. Proconvulsant and 'anxiogenic' effects of n-butyl beta carboline-3-carboxylate, an endogenous benzodiazepine binding inhibitor from brain. Pharmacol Biochem Behav. 1988;30:331-336. https://doi.org/10.1016/0091-3057(88)90463-7
  23. Oderinde O, Noronha C, Oremosu A, Kusemiju T, Okanlowan OA. Abortifacient properties of aqueous extract of Carica papaya (Linn) seeds on female Sprague- Dawley rats. Niger postgrad Med J. 2002;9:95-98.
  24. Oduola T, Adeniyi FAA, Ogunyemi EO, Idowu TO, Bello IS. Evaluation of the Effects of Intake of Extract of Unripe Pawpaw (Carica Papaya) on Liver Function in Sickle Cell Patients. World J Med Sci. 2007;2:28-32.
  25. Okoli RI, Aigbe O, Ohaju-Obodo JO, Mensah JK. Medicinal Herbs Used for Managing Some Common Ailments among Esan People of Edo State, Nigeria. Pakistan J Nutri. 2007;6:490-496. https://doi.org/10.3923/pjn.2007.490.496
  26. Olley BO. Is dried paw-paw leaf a psychoactive substance. IFI PsychologIA. 2007;15:25-39.
  27. Otsuki N, Dang NH, Kumagai E, Kondo A, Iwata S, Morimoto C. Aqueous extract of Carica papaya leaves exhibits anti-tumor activity and immunomodulatory effects. J Ethnopharmacol. 2010;127:760-767. https://doi.org/10.1016/j.jep.2009.11.024
  28. Owoyele BV, Adebukola OM, Funmilayo AA, Soladoye AO. Anti-inflammatory activities of ethanolic extract of Carica papaya leaves. Inflammopharmacol. 2008;16:168-173. https://doi.org/10.1007/s10787-008-7008-0
  29. Perveen T, Haider S, Kanwal S, Haleem DJ. Repeated administration of Nigella sativa decreases 5-HT turnover and produces anxiolytic effect in rats. Pak J Pharm Sci. 2009;22:139-144.
  30. Su C. Actions of nicotine and smoking on circulation. Pharmacol Ther. 1982;17:129-141. https://doi.org/10.1016/0163-7258(82)90050-X
  31. Walsh RN, Cummins RA. The open-field test: a critical review. Psychol Bullet. 1976;83:482-504. https://doi.org/10.1037/0033-2909.83.3.482