Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지
DOI QR코드

DOI QR Code

Antibacterial Activity and Nitric Oxide Production Inhibitory Activity of the Extract and its Fractions from the Leaves of Prunus sargentii

산벚나무 잎 추출물 및 분획물의 항균활성과 Nitric Oxide 생성억제 활성

  • Yang, Sun-A (Department of Oriental Medicine Materials, Dongshin University) ;
  • Pyo, Byoung-Sik (Department of Oriental Medicine Materials, Dongshin University) ;
  • Kim, Sun-Min (Department of Oriental Medicine Materials, Dongshin University) ;
  • Lee, Kyoung-In (Biotechnology Industrialization Center, Dongshin University)
  • 양선아 (동신대학교 한약재산업학과) ;
  • 표병식 (동신대학교 한약재산업학과) ;
  • 김선민 (동신대학교 한약재산업학과) ;
  • 이경인 (동신대학교 생물자원산업화지원센터)
  • Received : 2012.08.06
  • Accepted : 2012.08.31
  • Published : 2012.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to investigate the antibacterial activity against pathogens of acne and the anti-inflammatory effect of 75% ethanol extract and its fractions from the leaves of Prunus sargentii. In the antibacterial activity by the disc diffusion assay, the extract showed the highest effect against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis in 5 mg/disc. However, the ethyl acetate fraction showed the highest antibacterial activity in 1 mg/disc. On the other hand, the hexane and chloroform fraction showed strong nitric oxide (NO) production inhibitory effect in lipopolysaccharide (LPS)-stimulated Raw 264.7 cell. In the cell viability of Raw 264.7 by MTT assay, the extract and all fractions were exhibited normal viabilities as nontoxic result. Consequently, the extract from the leaves of P. sargentii and its ethyl acetate fraction could be applicable to functional materials for antibacterial activity related fields. Moreover, the hexane and chloroform fraction could be applicable to candidate materials as anti-inflammatory agent.

Keywords

References

  1. Ahn YG, Kim SK, Shin CS and Min JH. (2002). Inhibitory effects of wax gourd extract on melanin formation and acneforming bacterial growth. The Korean Journal of Food and Nutrition. 15:137-143.
  2. An BJ, Cho YJ, Son JH, Park JM, Lee JY and Park TS. (2006). Antioxidant effects and tyrosinase inhibition activity of extract of Prunus sargentii Rehder. Journal of the Korean Society for Applied Biological Chemistry. 49:145-148.
  3. Blois MS. (1958). Antioxidant determinations by the use of a stable free radical. Nature. 181:1199-1200. https://doi.org/10.1038/1811199a0
  4. Burton JL and Shuster S. (1971). The relationship between seborrhea and acne vulgaris. British Journal of Dermatology. 84:600-601.
  5. Choi DH, Lee HJ and Lee SS. (2003). Studies on biological activity of wood extractives (7)-antimicrobial and antioxidation activities of extractives from the heartwood of Prunus sargentii. Mokche Konghak. 31:16-23.
  6. Chung HT, Pae HO, Choi BM, Billiar TR and Kim YM. (2001). Nitric oxide as a bioregulator of apoptosis. Biochemical and Biophysical Research Communications. 282:1075-1079. https://doi.org/10.1006/bbrc.2001.4670
  7. Collins CH, Lyne PM and Grange JM. (1995). Collins and Lyne's microbiological methods. Butterworth-Heinemann Ltd. Jordan Hill, England. p.178-205.
  8. Cunliffe WJ, Holland DB and Jeremy A. (2004). comedone formation: etiology, clinical presentation and treatment. Clinics in Dermatology. 22:367-374. https://doi.org/10.1016/j.clindermatol.2004.03.011
  9. Dewick PM. (2002). Medicinal natural products. Wiley & Sons. Chichester, England. p.149-151.
  10. Ding AH, Nathan CF and Stuehr DJ. (1988). Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. The Journal of Immunology. 141:2407-2412.
  11. Haenen GR, Paquay JB, Korthhouwer RE and Bast A. (1997). Peroxynitrite scavenging by flavonoids. Biochemical and Biophysical Research Communications. 236:591-593. https://doi.org/10.1006/bbrc.1997.7016
  12. Han BH and Han YN. (1978). Immunosuppressant activity of cherry bark extract. Korean Journal of Pharmacognosy. 9:173-175.
  13. Im DY and Lee KI. (2011). Antioxidative, antibacterial activity and tyrosinase inhibitory activity of the extract and fractions from Taraxacum coreanum Nakai. Korean Journal of Medicinal Crop Science. 19:238-245. https://doi.org/10.7783/KJMCS.2011.19.4.238
  14. Jeoung YJ, Choi SY, An CS, Jeon YH, Park DK and Lim BO. (2009). Comparative effect on anti-inflammatory activity of the Phellinus linteus and Phellinus linteus grown germinated brown rice extracts in murine macrophage RAW 264.7 cells. Korean Journal of Medicinal Crop Science. 17:97-101.
  15. Jung HA. (2002). Antioxidant constituents from the leaves of Prunus serrurata var. spontanea. Ph. D. Thesis. Pukyung National Universty. p.6-16.
  16. Kam WS. (1981). Pharmaceutical botany. National Chinese Medicine Institute. p.305-306.
  17. Kang GJ. (2007). Inhibitory effect of organic extracts from Prunus yedoensis Matsum barks on the atopic dermatitis-like inflammation. Master Thesis. Cheju National University. p.56-60.
  18. Ki HG, Yun SJ, Lee JB, Kim SJ, Lee SC and Won YH. (2005). Microorganism isolated from acne and their antibiotic susceptibility. Korean Journal of Dermatology. 43:871-875.
  19. Kim HJ. (2005). Development of tea using flower and young leaf of oriental cherry(Prunus serrurata var. spontanea). Master Thesis. Wonkwang University. p.11-12.
  20. Kim HJ, Heo BK, Baek SH, Park YS and Park YJ. (2006a). Effects of the tea manufacture method on mineral contents and sensory evaluation in flower and young leaf of Prunus serrulata lindl. var. spontanea Max. People, Plants and Environment. 9:26-33.
  21. Kim HJ, Park YJ, Bea KS and Heo BG. (2006b). Component analysis of petals and young leaves for oriental cherry plants. People, Plants and Environment. 9:20-25.
  22. Kim TJ. (1996). Korean resources plants-II. Seoul University Press. Seoul, Korea. p.168-169.
  23. Kim YH. (1999). The characterization of anthocyanin pigments prepared from cherry(Prunus serrulata L. var. spontanea Max. wils.) for the potential sources of red colorant. Journal of the Korean Society for Applied Biological Chemistry. 42:134-139.
  24. Lee ES, Ju HK, Moon TC, Lee E, Jang Y, Lee SH, Son JK, Baek SH and Chang HW. (2004). Inhibition of nitric oxide and tumor necrosis factor-a(TNF-a) production by propenone compound through blockade of nuclear factor(NF)-B activation in cultured murine macrophage. Biological & Pharmaceutical Bulletin. 27:617-620. https://doi.org/10.1248/bpb.27.617
  25. Lee HJ, Lee SS, Choi DH and Atsushi K. (2001). Studies on biological activity of wood extractives(VI)-flavonoids in heartwood of Prunus sargentii. Mokchae Konghak. 29:133-139.
  26. Lee HJ, Lee SS and Choi DH. (2003). Studies on biological activity of wood extractives(VII)-Antimicrobial and antioxidative activities of extractives from the heartwood of Prunus sargentii. Mokchae Konghak. 31:16-23.
  27. Lee KI, Yang SA, Pyo BS and Kim SM. (2011a). Antibacterial activity against pathogens of acne and tyrosinase inhibitory activity of extract and fractions from bark of Prunus sargentii. Korean Journal of Pharmacognosy. 42:155-160.
  28. Lee SE, Lee JH, Kim JK, Kim KS, Kim YO, Soe JS, Choi JH, Lee ES, Noh HJ and Kim SY. (2011b). Anti-inflammatory activity of medicinal plant extracts. Korean Journal of Medicinal Crop Science. 19:217-226. https://doi.org/10.7783/KJMCS.2011.19.4.217
  29. Lin JY, Li CY and Hwang IF. (2008). Characterization of the pigment components in red cabbage(Brassica oleracea L. var.) juice and their anti-inflammatory effects on LPS-stimulated murine splenocytes. Food Chemistry. 109:771-781. https://doi.org/10.1016/j.foodchem.2008.01.039
  30. Liu RH. (2004). Potential synergy of phytochemicals in cancer prevention : mechanism of action. Journal of Nutrition. 134:3479S-3485S.
  31. Manach C, Williamson G, Morand C, Scalbert A and Remesy C. (2005). Bioavailability and bioefficacy of polyphenols in humans. American Journal of Clinical Nutrition. 81:230S-242S.
  32. Marcocci L, Maguire JJ, Droy-Lefaix MT and Packer L. (1994). The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochemical and Biophysical Research Communications. 201:748-755. https://doi.org/10.1006/bbrc.1994.1764
  33. Moreno MI, Isla MI, Sampietro AR and Vattuone MA. (2000). Comparison of the free radical-scavenging activity of propolis from several region of Argentina. Journal of Ethnopharmacology. 71:109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  34. Oh YJ, Seo HR, Choi TM and Jung DS. (2010). Evaluation of antioxidant activity of the extracts from the aerial parts of Cnidium officinale Makino. Korean Journal of Medicinal Crop Science. 18:373-378.
  35. Otto F and Denis W. (1912). On phosphotungsticphosphomolybdic compounds as color reagents. Journal of Biological Chemistry. 12:239-243.
  36. Park ES, Shin MK and Song HJ. (1998). A study on the antiallergic effect of Cortex Betula Platyphyllae or Cortex Pruni Serrulatae extract. Korean Journal of Herbology. 13:57-68.
  37. Park JM, Lee JY, Park TS, Hyun SJ, Kim HH, Cho YJ, Kwon OJ, Son AR, Kim DS and An BJ. (2008a). A study on the cosmeceutical activities of Prunus sargentii R. Journal of the Korean Society for Applied Biological Chemistry. 51:70-78.
  38. Park JM, Lee JY, Park TS, Park GH, Park KS, Kim TH, Cho YJ, Kwon OJ, Choi KI and An BJ. (2008b). Biological activity investigation, and phenol compounds isolation from barks of Prunus sargentii R. Korean Journal of Medicinal Crop Science. 16:173-182.
  39. Park YJ, Kim HJ and Heo BG. (2007). Anti-microbial, antioxidant and anti-inflammation effects with of the flower and the young leaf extracts in oriental cherry plants. People, Plants and Environment. 10:43-49.
  40. Radi R, Beckman JS, Bush KM and Freeman BA. (1991). Peroxynitrite oxidation of sulfhydryls, the cytotoxic potential of superoxide and nitric oxide. Journal of Biological Chemistry. 266:4244-4250.
  41. Shin MK. (2006). Clinical traditional herbalogy. Younglimsa. Seoul, Korea. p.399-400.
  42. Yang SA, Cho JH, Pyo BS, Kim SM and Lee KI. (2012). Comparison of the physiological activities of extracts from different parts of Prunus sargentii. Korean Journal of Medicinal Crop Science. 20:159-164. https://doi.org/10.7783/KJMCS.2012.20.3.159
  43. Yoon TS, Cheon MS, Kim SJ, Lee AY, Moon BC, Chun JM, Choo BK and Kim HK. (2010). Evaluation of solvent extraction on the anti-inflammatory efficacy of Glycyrrhiza uralensis. Korean Journal of Medicinal Crop Science. 18:28-33.

Cited by

  1. Cultivation Characteristics and Variation of Artemisinin Contents by Harvest Time of Artemisia annua L. Distributed in Korea vol.21, pp.6, 2013, https://doi.org/10.7783/KJMCS.2013.21.6.433
  2. Anti-inflammatory Activity of Solvent Fractions from Ginseng Berry Extract in LPS-Induced RAW264.7 Cells vol.22, pp.6, 2014, https://doi.org/10.7783/KJMCS.2014.22.6.449
  3. Antioxidant Activities of Various Berries Ethanolic Extract vol.23, pp.1, 2015, https://doi.org/10.7783/KJMCS.2015.23.1.49
  4. Screening for Inhibition Activity of Plant Extracts on Microorganism Contaminating in Cosmetics vol.23, pp.1, 2015, https://doi.org/10.7783/KJMCS.2015.23.1.57
  5. The Effect of Prunus sargentii R. Seed Oil on the Lipid Profile in Serum in Mice vol.26, pp.4, 2013, https://doi.org/10.9799/ksfan.2013.26.4.670
  6. Antibacterial and Nitric Oxide Production Inhibitory Activities of Prunus sargentii Branches Extract and Its Fractions against Pathogens of Acne vol.24, pp.2, 2016, https://doi.org/10.7783/KJMCS.2016.24.2.129