Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Preparation of Camel Milk Liposome and Its Anti-Aging Effects

낙타유가 함유된 리포좀 제조 및 피부 노화 개선 효과 연구

  • 최성규 ((주)더마랩 기술 연구소) ;
  • 박근동 ((주)더마랩 기술 연구소) ;
  • 김다애 ((주)더마랩 기술 연구소) ;
  • 이대우 ((주)더마랩 기술 연구소) ;
  • 김윤정 ((주)더마랩 기술 연구소)
  • Received : 2014.01.23
  • Accepted : 2014.02.28
  • Published : 2014.06.30
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Abstract

In this study, in order to know the application for cosmetic ingredient, the liposome contained camel milk was prepared and tested in human skin fibroblast. Collagen and hyaluronan synthase-3 (HAS-3) gene expression were increased by camel milk liposome in a concentration-dependent manner, whereas elastase activity and matrix metalloproteinase (MMP)-1 gene expression were inhibited. We also found that camel milk liposome regenerated UVB-damaged fibroblast. As the results, we suggest that the liposome contained camel milk is applicable for a potential cosmetic ingredient to improve anti-aging effect.

본 연구에서는 낙타유를 유효성분으로 하여 리포좀을 제조하였고, 이를 이용하여 항노화 효능을 갖는 화장품 원료를 개발하고자 다양한 실험을 실시하였다. 제조된 낙타유 리포좀은 피부 섬유아세포에서 collagen과 hyaluronan synthase-3 (HAS-3)의 발현을 증가시키고 matrix metalloproteinase (MMP)-1의 발현을 감소시킬 뿐 아니라 elastase의 활성을 억제하여 주름 개선 기능을 갖는 것을 확인하였다. 또한 자외선으로부터 손상된 세포를 재생시키는 효과를 확인하였다. 이에 따라 낙타유를 함유한 리포좀은 항노화 소재로 활용할 수 있을 것으로 사료된다.

Keywords

References

  1. H. C. Choi and C. H. Oh, Evaluation of skin furrows in the ageing process using an image analysis system, Ann. Dermatol., 35, 292 (1997).
  2. H. M. Chiang, H. C. Chen, T. J. Lin, I. CShih, and K. C. Wen, Michelia alba extract attenuates UVB-induced expression of matrix metalloproteinases via MAP kinase pathway in human dermal fibroblasts, Food and Chemical Toxicology, 50, 4260 (2012). https://doi.org/10.1016/j.fct.2012.08.018
  3. J. M. Yoo, Y. J. Kang, H. B. Pyo, E. S. Choung, S. Y. Park, J. H. Choi, G. J. Han, C. H. Lee, and T. J. Kim, Anti-wrinkle effects of korean rice wine cake on human fibroblast, J. Life Sci., 20(12), 1838 (2010). https://doi.org/10.5352/JLS.2010.20.12.1838
  4. S. H. Jang, J. M. Chun, E. M. Shin, H. J. Kim, and Y. S. Kim, Inhibitory effects of curcuminoids from curcuma longa on matrix metalloproteinase-1 expression in keratinocytes and fibroblasts, Dermatol., 40(3) (2010).
  5. E. Houben, K. De Paepe, and V. Rogiers, A keratinocyte's course of life, Skin Pharmacol. Physi., 20, 122 (2007). https://doi.org/10.1159/000098163
  6. W. M. Yang, H. M. Kim, M. S. Chang, W. S. Park, W. N. Kim, S. W. Kim, D. G. Choi, H. C. Lee, Y. K. Kim, and S. K. Park, Effects of ethanol extract of Nelumbo nucifera leaves on anti-oxidation and type I procollagen expression in CCD-986sk cells, Kor. J. Ori. Med. Prescription, 14(2), 67 (2006).
  7. P. Dahiya and R. Kamal, Hyaluronic acid: A boon in periodontal therapy, North American J. Med. Sci., 5(5), 309 (2013). https://doi.org/10.4103/1947-2714.112473
  8. R. Rettenmaier and D. ATKINS, Vitamin content of camel milk, Internat. J. Vit. Nutr. Res., 62, 30 (1992).
  9. S. M. Shamsia, Nutritional and therapeutic properties of camel and human milks, Int. J. Genetics and Molecular Biol., 1(2), 52 (2005).
  10. R. Ronald, Camel milk: characteristics and perspectives for use in clinical practice, Revista chilena de nutricion, 38(2), 211 (2011). https://doi.org/10.4067/S0717-75182011000200011
  11. L. Tang, J. Wu, Q. Ma, T. Cui, F. M. Andreopoulos, J. Gil, J. Valdes, S.C. Davis, and J. Li, Human lactoferrin stimulates skin keratinocyte function and wound re-epithelialization, British J. Derm., 163, 38 (2010).
  12. L. Pinto, G. Rodriguez, and R. AM, Effect of cholesterol and ethanol on dermal delivery from DPPC liposomes, Int. J. Pharm., 298, 1 (2005). https://doi.org/10.1016/j.ijpharm.2005.02.021
  13. K. H. Jung and H. J. Kim, Development of an agar diffusion method to measure elastase inhibition activity using elastin-congo red, J. Microbiol. Biotechnol., 16(8), 1320 (2006).
  14. S. H. Park, E. J. Kim, S. H. Park, K. H. Son, S. J. Yang, S. M. Kim, S. R. Park, Y. H. Kim, G. H. Gong, and M. H. Woo, K, Guideline for analytical method of preservatives and sunscreen ingredients in cosmeticsanalysis of arbutin and adenosine in cosmetics, KFDC, 5, 23 (2007).
  15. T. J. Kang, Cause of skin aging, BRIC BioWave, 13, 3 (2011).
  16. G. Badr, Camel whey protein enhances diabetic wound healing in a streptozotocin-induced diabetic mouse model: the critical role of ${\beta}$-Defensin-1, -2 and -3, Lipids in Health and Disease, 12(1), 46 (2013). https://doi.org/10.1186/1476-511X-12-46
  17. M. Hosam, H. Wissam, R. Schneider, and M. Hassan, Camel milk lactoferrin reduces the proliferation of colorectal cancer cells and exerts antioxidant and DNA damage inhibitory activities, Food Chemistry, 141, 148 (2013). https://doi.org/10.1016/j.foodchem.2013.03.039