Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Extraction of Isoflavones from Korean Soybean by Sub/Supercritical Water

아임계/초임계 수에 의한 토종콩으로부터 이소플라본의 추출

Han, Soon-Koo;Lee, Kwang-Jin;Kim, Jae-Duck;Lee, Youn-Woo;Row, Kyung-Ho
한순구;이광진;김재덕;이윤우;노경호

  • Published : 2004.12.31
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Abstract

Aglycones compounds were extracted from Korean soybean by sub/supercritical water. The various experimental variables were the temperature and pressure of sub/supercritical water. The extracted sample was analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). Aglycons were selectively extracted at the experimental condition of $200^{\circ}C$ and 200 bar of subcritical water. It was also experimentally observed that almost the same amount of aglycones, $0.9{\mu}g$, was extracted from 5 g of Korean soybean at $400^{\circ}C$ and 250 bar of supercritical water.

토종콩으로부터 비배당체 화합물을 아임계/초임계 수(水)를 이용하여 추출하였다. 이 때, 실험 조건은 아임계 및 초임계 수의 온도와 압력으로 하였다. 추출된 용액은 역상 고성능 액체 크로마토그래피(RP-HPLC)를 사용하여 분석하였다. $200^{\circ}C$, 200 bar의 아임계 상태의 실험 조건에서 비배당체들이 선택적으로 추출되었다. 또한 $400^{\circ}C$, 250 bar의 초임계 상태에서 5 g의 토종콩으로부터 추출된 비배당체들의 양(0.9${\mu}g$)과 비슷한 것을 실험을 통해 확인하였다.

Keywords

References

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