• Title, Summary, Keyword: vitamins

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Microbiological Assay of Vitamin B group in Panax Ginseng Roots II. Assay of Pantothenic Acid and Biotin (인삼중 Vitamin B군의 미생물학적검정 II Pantothenic acid 및 Biotin 의 검정)

  • 김영은;허무언
    • YAKHAK HOEJI
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    • v.8 no.3
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    • pp.85-88
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    • 1964
  • Pantothenic acid and biotin contents in Panax Ginseng roots were determined microbiologically with L. arabinosus 17-5. Detection of the vitamins was achieved by the thin-layer chromatography and PPC. Pantothenic acid and biotin were found at the Rf values of 0.42 and 0.55 respectively on the thin-layer chromatograms. In order to find out whether or not the L. arabinosus growth promoting factors contained in the respective ginseng extracts, as shown by the microbiological assay, were really the vitamins respectively, PPC was carried out. Microbiological assay of the vitamins met with results that the average pantothenic acid content in the roots was 6.6r/g, calculated as Ca-pantothenate, and the average biotin content 9.24 mr/g.

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Study for Major Nutrients Sources of Food by Korean Nutrition Survey -II. Minerals and Vitamins- (국민영양조사를 이용한 영양소별 주요 공급식품에 관한 연구 -II. 무지길 및 비타민-)

  • 박미아
    • Journal of Nutrition and Health
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    • v.30 no.1
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    • pp.91-99
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    • 1997
  • We have studied the major food sources of minerals and vitamin based on the data from the National Nutrition Survey in 1993. The most important food sources was anchovy for calcium, spinach for vitamin A, and kimchi for vitamin C. The primary source of iron, thiamin, riboflavin and niacin was rice. Number of foods providing 90% of individual nutrient intake were 54 items for calcium, 40 items for iron, 20 items for vitamin A, 25 items for thiamin, 29 items for riboflavin, 39 items for niacin and 18 items for vitamin C. Among regions, amount of nutrients from same foods(10 more important food source) didn't show any significant differences (p>0.05) in minerals and vitamins studied. Over all rice was found to be the most important sources of minerals and vitamins.

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Study on the Additional Effect of Fat-Soluble Vitamins to Antioxidative Action of Ginseng Saponin (인삼(人蔘)사포닌의 항산화작용(抗酸化作用)에 대한 지용성(脂溶性) 비타민의 첨가효과(添加效果))

  • Lee, Hyang Sook;Woo, Soon Im;Choi, Jin Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.12 no.2
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    • pp.110-115
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    • 1983
  • This study was to demonstrate the additional effect of fat-soluble vitamins on inhibitory action of Korean ginseng (Panax ginseng C. A. Meyer) saponin to lipoperoxide formation in vitro and in vivo. The ginseng saponin and vitamins were added to the substrate of linoleic acid and incubated on a shaking water-bath at $60^{\circ}C$, and the inhibitory action on lipoperoxide formation was examined by measuring the TBA value (532 nm), POV (500 nm) and EDA (electorn donating ability to ${\alpha}$, ${\alpha}$-diphenyl-${\beta}$-picrylhydrazyl at 525nm) for in vitro. The ginseng saponin and vitamins were administered to Sprague-Dawley rats (♂, 100~120g) orally, and the inhibitory effect on lipoperoxide formation was examined by measuring the TBA value in vivo. EDA to DPPH of ginseng saponin added with vitamin A and D were higher than that of ginseng saponin only. Ginseng saponin added with vitamin A and D inhibited strongly lipoperoxide formation of initial step by extension of induction period in vitro. Fat-soluble vitamins such as vitamin E and A were approved the additional effect on inhibitory action of lipoperoxide formation in vitro. The additional effect of fat-soluble vitamins to ginseng saponin for inhibitory action of lipoperoxide formation was effective vitamin E and D for blood, vitamin A and E for liver in vivo.

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Modulation Effects of Antioxidant Vitamins on Ochratoxin A-induced Oxidative Toxicity in Mice (마우스에서 Ochratoxin A로 유발된 산화적 독성에 대한 항산화 비타민의 완화작용)

  • Park, Jung-Hyun;Kang, Sung-Jo;Kang, Jin-Soon;Ryu, Jae-Chun;Chung, Duck-Hwa
    • Korean Journal of Food Science and Technology
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    • v.31 no.3
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    • pp.831-837
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    • 1999
  • Ochratoxin A (OA), a naturally occurring mycotoxin, has been known to cause renal and hepatic lesion in human and animals. This study was carried out to investigate the modulation effects of antioxidant vitamins on OA-induced lipid peroxidation associated with oxidative damage. Vitamin C (10 mg/kg/day) and vitamin E (63.8 mg/kg/day) were administered by intraperitoneal (i.p.) injection to male ICR mice, and 1 hr later, OA which was dissolved in 0.1 M $NaHCO_3$, treated 4 mg/kg/day by i.p. injection. During 4 days repeated, and then measured superoxide dismutase (SOD) activity, catalase activity and malondialdehyde (MDA) formation in microsomes of liver and kidney. Additionally, the relationship between cell damage and modulation effects of antioxidant vitamins was evaluated by comet assay. Results were as followed; i) SOD, catalase activity and MDA level were significantly increased by OA treated, ii) SOD, catalase activity and MDA formation were significantly decreased by antioxidant vitamins combine treated, iii) blood cell damage associated with lipid peroxidation, induced by OA, also modulated by antioxidant vitamins. These results indicated that antioxidant vitamins might be used for prevention of renal and hepatic damage due to ochratoxicosis.

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Effects of Temperature and Relative Humidity on Water Soluble Vitamin Contents in Commercial Vitamin Tablet (저장 온도 및 상대습도가 비타민 정제 중 수용성 비타민 함량의 변화에 미치는 영향)

  • Lee, Jae-Hwang;Kim, Sae-Gon;Lee, Dong-Un;Park, Seok-Jun;Lee, Jin-Hee;Lee, Kang-Pyo;Kim, Dong-Seob;Choi, Sung-Won;Baik, Moo-Yeol
    • Korean Journal of Food Science and Technology
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    • v.37 no.6
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    • pp.1028-1034
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    • 2005
  • Effects of temperature and relative humidity on contents of water-soluble vitamins (vitamins $B_1,\;B_2,\;B_3,\;B_6$, and C) of two commercial tablets ("Multivitamin Dandelion" and "Chewable vitamin C") were investigated. When stored at various temperatures (25, 35, and $45^{\circ}C$) with cap, all measured vitamins were stable and degraded very slowly during 24 weeks of storage; low relative humidity (11% RH) without cap also provided stability during storage period. At higher relative humidities (75 and 100% RH), contents of all water-soluble vitamins, except vitamins $B_2\;and\;B_3$, decreased significantly at early storage period. These results showed that stability of water-soluble vitamins is highly dependent on relative humidity rather than storage temperature.

Nutritional Reguirements for Growth of Cellulomonas flavigena on cellulosic substrates (Cellulose기질에서 cellulomonas flavigena의 생장에 대한 영양요구성)

  • 한윤우
    • Korean Journal of Microbiology
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    • v.16 no.4
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    • pp.155-160
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    • 1978
  • Nutritional requirements for the growth of Cellulomonas flavigena were studied. C. flavigena grew well on cellulose when 0.005% or more of yeast extract was present in the growth medium. The growth factor in yeast extrct was, in part, thiamine and biotin. Amino acids had little effect on the growth on the organism. The extent of growth on yeast extract was much higher than that obtained on those vitamins, which indicates the presence of growth factors in yeast extract besides the vitamins, among the carbohydrates tested, the organism grew best on glucose and galactose, and the optimum N/P ratio was within the range of 0.75~3.17.

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Some Influences of Heating upon Constituents of Raw Milk (처리 온도가 우유 성분에 미치는 영향)

  • Cho, J.H.;Cho, T.H.
    • Korean Journal of Veterinary Research
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    • v.10 no.2
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    • pp.89-92
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    • 1970
  • The effects of heat treatment of milkon the contents of nutrients, minerals and vitamins were studied by the rontine analytical method. The results obtained were: calcium acid 117.5mg, phosphorus 62.61mg and iron 112.8ug. The average constituents of rowmilk tested were not affected by heating except vitamins.

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Nutritional Status of Antioxidant Vitamins in the Elderly Living in Ulsan Metropolitan City

  • Kim, Jung-Hee;Kim, Mi-Joung;Kim, Ok-Hyun
    • Journal of Community Nutrition
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    • v.3 no.2
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    • pp.120-126
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    • 2001
  • Body antioxidant status is an important factor for the prevention of many chronic diseases in the elderly. This study was done to evaluate the nutritional status of antioxidant vitamins of the elderly by determining their intakes and plasma levels. It was also aimed to compare daily intakes and plasma levels of antioxidant vitamins by sex of age. Subjects were 225 elderly persons aged over 60 years old (63 males, 162 females) living in Ulsan area. Subjects were divided by groups according to age(< 65, 65-74, 75) and sex. Dietary Intakes were assessed by semi-quantitative food frequency questionnaires(FFQ). Plasma Vitamin C level was measured by 2,4-dinitrophenylhydrazine method and plasma levels of vitamin E, A and ${\beta}$-carotene were measured by HFLC. The average intakes of vitamin C were 104.9g(150% of RDA) and 104.4g(149% of RDA) in the elder1y males and females, respectively. Vitamin C intake of the elderly was significantly decreased by aging but not different by sex. The average intakes of vitamin A were 678$\mu\textrm{g}$RE(96.9% of RDA) and 604$\mu\textrm{g}$:RE(86.2% of RDA) in elderly males and females, respectively. The average levels of Plasma vitamin C were 6.22mg/L and 11.45mg/L in the elderly males and females, respectively. Those levels are within normal range(6-20mg/L). However the percentage of the elderly males with deficiency(< 2mg/L) and marginal level(2-4mg/L) of vitamin C were 27.4% and 16.1%, respectively. Plasma retinaol levels were 0.39mg/L for the elderly males and 0.37mg/L for the elderly females, which were within normal range. But the percentage of subjects with marginal level were 28% ill both males and females. Plasma ${\alpha}$-tocopherol levels of the elderly were lower than normal range(5-12mg/L). Plasma levels of vitamin C, E and ${\beta}$-carotene, except retinol, were significantly higher in the elderly females compared to males and showed significant decrease by aging.

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Effect of Dietary Vitamin A and E Peroxidative Deterioration of Heart in Adriamycin-Treated Rats (식이중의 Vitamin A와 E가 Adriamycin을 투여한 흰쥐의 심근손상의 원인인 과산화작용에 미치는 영향)

  • 정영아
    • Journal of Nutrition and Health
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    • v.25 no.5
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    • pp.379-388
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    • 1992
  • The present study was designed to evaluate the protective effects of dietary vitamin A or E. and of combination of vitamins A and E on peroxidative deterioration of heart in adriamycin-treated rats. Male Sprague-Dawley rats were assigned to 5 groups according to the dietary supplementation of vitamin A or E Except control rats a dose of 2mg ADR/kg of B. W was injected to these animals intraperitoneally on the same day every week. Adriamycin treatment significantly decreased the weight gain of experimental rats compared with that of control rats, But this decrement was not modified by dietary supplementation of vitamin A or E. Lipid peroxide values of plasma were elevated by ADR treatment. The combined use of ADR and dietary vitamin A or E significantly reduced these values, The interaction between vitamins A and E seemed to be present in the lipid peroxide value of plasma. Catalase and superoxide dismutase(SOD) activities in rat heart were decrased by ADR treatment but glutathione peroxidase(GSH-Px) activity was elevated. Dietary supplmen-tation of vitamin A or E enhanced the heart catalase and SOD activities. except only vitamin A-supplemented group. GSH-Px activity of rat heart tended to be decreased by dietary supple-mentation of vitamin A or E. With ADR treatment polyunsaturated fatty acids such as archido-nic acid(20:4) and docosahexaenoic acid(22:6) were decreased in rat heart. However dietary supplementation of vitamins A and E reduced this decrease. The retinol and tocopherol contents of rat plasma were decreased by ADR treatment. Dietary vitamin A or E influence vitamin A or E content of plasma. The interaction between dietary vitamins A and E was observed in vitamin A or E level of rat plasma.

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Effects of pH, Sucrose and Vitamins on the Growth and Tropane Alkaloid Production of Hairy Roots of Datura stramonium var. tatula Torr. (독말풀(Datura stramonium var. tatula Torr.) 모상근의 성장과 tropane alkaloid 생성에 미치는 pH, 서당 및 비타민의 영향)

  • 양덕조;강현미;이강섭;김용해;양덕춘
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.3
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    • pp.143-148
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    • 1997
  • The optimum concentrations of pH, sucrose and vitamins for the growth and tropane alkaloid production of hairy root clone DTLA9 (best growth line) were investigated. The optimum pH in growth and tropane alkaliod production of DTLA9 clone in SH (Schenk and Hildebrandt, 1972) basal medium without growth regulator were pH 6.3 and 6.5, respectively. Also, the optimum sucrose concentration in growth and tropane alkaliod production in the same medium were 3.0 and 2.8%, respectively. The optimum concentrations of ascorbic acid, D-pantothenate, nicotinic acid, pyridoxine, riboflavin, and thiamine on the growth of DTLA9 clone in SH basal medium without vitamins were 0.1 mM, 0.003 mM, 0.07 mM, 0.002 mM, 0.025 mM, and 0.01 mM, respectively. In particular, supplement of 0.1 mM ascorbic acid to SH basal medium without vitamins stimulated the tropane alkaloid production.

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