• Title, Summary, Keyword: viability

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Studies on the viability and infectivity of Fasciola hepatica metacercariae (간질(Fasciola hepatica) 피낭유충의 생존성 및 감염성에 관한 연구)

  • Kim, Ji-ho;Kim, Jong-taek;Cho, Shin-hyeong;Lee, Chung-gil
    • Korean Journal of Veterinary Research
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    • v.38 no.1
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    • pp.161-166
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    • 1998
  • Fasciola hepatica eggs were obtained from the bile of infected cattle at a local abattoir. Metacercariae(MC) were produced using Lymnaea viridis, the intermediate host of the parasite. They were stored in distilled water at refrigerator($3{\sim}5^{\circ}C$) and at room temperature($22{\sim}27^{\circ}C$). The viability and infectivity of the MC were determined at monthly intervals for 12 months. The viability was determined by both microscope and excystation, and the infectivity by infecting mice. The MC stored at room temperature had a high viability up until 60 days, and thereafter the viability declined rapidly ; at day 120, only 2.5% of the MC were excysted. Most of the MC stored at refrigerator retained the viability up until 90 days, and thereafter the viability declined slowly ; about half of them were viable at day 210 and 5% of them retained the viability until day 270. The survival rates of the MC determined by microscope were always higher than those determined by excystation(p<0.05). The infectivity of the MC wisely followed the viability at the two different storage temperatures. Most of the mice infected orally with the MC died within 3-9 weeks of acute fasciolosis.

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Phosphate-Induced Rat Vascular Smooth Muscle Cell Calcification and the Implication of Zinc Deficiency in A7r5 Cell Viability

  • Shin, Mee-Young;Kwun, In-Sook
    • Preventive Nutrition and Food Science
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    • v.18 no.2
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    • pp.92-97
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    • 2013
  • The calcification of vascular smooth muscle cells (VSMCs) is considered one of the major contributors for vascular disease. Phosphate is known as the inducer for VSMC calcification. In this study, we assessed whether phosphate affected cell viability and fetuin-A, a calcification inhibitor protein, both which are related to VSMC calcification. Also, VSMC viability by zinc level was assessed. The results showed that phosphate increased Ca and P deposition in VSMCs (A7r5 cell line, rat aorta origin). This phosphate-induced Ca and P deposition was consistent with the decreased A7r5 cell viability (P<0.05), which implies phosphate-induced calcification in A7r5 cells might be due to the decreased VSMC cell viability. As phosphate increased, the protein expression of fetuin-A protein was up-regulated. A7r5 cell viability decreased as the addition of cellular zinc level was decreased (P<0.05). The results suggested that zinc deficiency causes the decreased cell viability and it would be the future study to clarify how zinc does act for VSMC cell viability. The results suggest that the decreased VSMC viability by high P or low Zn in VSMCs may be the risk factor for vascular disease.

Comparison of Seed Viability Among 42 Species Stored in a Genebank

  • Lee, Ho-Sun;Jeon, Young-Ah;Lee, Young-Yi;Lee, Sok-Young;Kim, Yeon-Gyu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.4
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    • pp.432-438
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    • 2013
  • This study was conducted to compare seed viability among 42 species after ten years of storage in the midterm storage complex ($4^{\circ}C$, 30-40% RH) at the National Agrobiodiversity Center (NAC) Korean genebank maintained by the Rural Development Administration (RDA), Republic of Korea and to suggest the relative seed longevity and suitable monitoring intervals. The germination data from initial tests and after ten years of storage were compared to measure changes in viability during storage. The decline in seed viability varied greatly among seeds from -11.5% for Triticum sp. to 80% for melon. Coriander, crowndaisy, safflower, cosmos, Chinesebellflower, waxgourd, melon, castorbean, Welch-onion, hollyhock, wild barley, and tallfescue showed significant decreases in viability of 34.2%, 73.4%, 36.5%, 30.0%, 40.2%, 71.3%, 80.0%, 65.9%, 45.5%, 51.4%, 53.0%, and 33.5%, respectively. Gardenpea, soybean, perilla, onion, wild rice, Italian-ryegrass, and pepper showed a 15-30% decline in viability, while the viability of morningglory, adzukibean, maize, and Capsicum sp. decreased by 15% to 5%. Chicory, radish, Chinese-cabbage, bottlegourd, watermelon, cucumber, pumpkin, Cucurbita sp., groundnut, kidneybean, clubwheat, sesame, wheat, Triticum sp., rice, barley, orchardgrass, buckwheat, and wild tomato showed changes in viability of <5%. The changes in storage viability also varied within families. The wild types of rice and barley showed rapid viability loss and presented different aspects from cultivars. Since seed viability of species, classified as index 1 or 2, showed germination losses >15% after ten years of storage, a viability test should be conducted with five year intervals, while species with germination loss of <15% (in index 3 or 4) can be retested at ten year intervals.

Inhibitory Effect of TREK Channel Blockers on Sperm Viability and Motility of Korean Native Bull (TREK통로 차단제의 한우 정자 생존성 및 운동성 억제 효과)

  • Kang, Dawon;Kim, Eun-Jin;Han, Jaehee
    • Journal of Embryo Transfer
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    • v.32 no.3
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    • pp.243-248
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    • 2017
  • Antioxidants have been added to cryoprotectant or in vitro culture medium for sperm to reduce the detrimental damage, such as reactive oxygen species. However, curcumin, an antioxidant, shows dual effect on the viability and progressive motility of bovine sperm exposed to hydrogen peroxide. Low concentration of curcumin increases sperm viability and progressive motility, whereas high concentration of curcumin reduces them. This study was performed to identify whether TREK-1 channel is related to low sperm viability and motility induced by high concentration of curcumin. Curcumin reduced TREK-1 channel activity in a dose-dependent manner. TREK-1 channel was expressed in sperm obtained from Korean native bull. Treatment with TREK-1 channel blockers, such as curcumin, fluoxetine, $GdCl_3$, and spadin, significantly reduced sperm viability and motility (p < 0.05). However, TREK-1 channel activators showed different effect; linoleic acid showed an increase in sperm viability and motility, and wogonin did not affect them. These results show that TREK-1 channel is involved in the regulation of sperm viability and motility. In particular, high concentration of curcumin might reduce sperm viability and progressive motility of Korean native bull through blockage of TREK-1 channel.

Effects of Cryoprotectants and Equilibration Time on the Viability of Frozen-thawed Porcine Oocytes (동결-융해된 돼지난포란의 생존성에 대한 항동해제와 평형시간의 영향)

  • 이장희;김창근;박충생
    • Journal of Embryo Transfer
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    • v.12 no.3
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    • pp.315-324
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    • 1997
  • This study was undertaken in an effort to develop a cryopreservation system of immature and mature porcine oocytes. For this aim, the experiments were designed to examine the effect of cryoprotectants and equdibration time on the viability of frozen-thawed oocytes by using trypan blue(TB) and fluorescene diacetate(FDA) test. The viability of frozen immature oocytes evaluated by TB test was slightly higher than that of frozen mature oocytes. The viability(25.O%) after IVM of frozen-thawed immature oocytes greatly decreased that(42.9%) of oocytes just after thawing, but it was higher than frozen-thawed mature oocytes(15.8%). When immature oocytes were equilibrated for 10, 20 and 30 minutes before freezing the oocyte viability was 20.0, 31.3 and 42.9%, respectively. There was a tendency for long equilibration before oocyte freezing to be more effective for the immature oocytes and a short equilibration time for mature oocytes. Although there was no difference in viability index of frozen oocytes hetween the viability test methods, the index of TB test was slightly higher than that of FDA test. The viability(FDA test) of frozen-immature oocytes with 3 different crtoprotectants was 22.2% for propylene glycol(PG), 9.3% for polyehtylene glycol(PEG) and 65.6% for PG+PEG, in which PG+PEG was more protective against freezing effect.

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Effect of Seaweed Extracts on the Viability of the Crustose Coralline Lithophyllum yessoense

  • Kang Se-Eun;Park Sun-Mee;Choi Jae-Suk;Ahn Dong-Hyun;Kim Young-Dae;Hong Yong-Ki
    • Fisheries and aquatic sciences
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    • v.8 no.4
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    • pp.243-246
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    • 2005
  • The addition of seaweed extracts was found to regulate the viability of cultures of the crustose coralline alga Lithophyllum yessoense. The viability was quantitated using a triphenyltetrazolium chloride assay, and the methanol-soluble extracts from 18 prevalent seaweed species were tested. Extracts from Codium fragile and Enteromorpha linza inhibited viability, and a Hizikia fusiform is extract slightly increased viability. The methanol extract of C. fragile, which had the strongest inhibitory activity, decreased viability to 72 or $52\%$ that of the control following addition of 0.2 or 2 mg/mL of extract to the culture, respectively. The main active compound in the C. fragile was lipid. This information is a preliminary result related to the exploration of seaweed restoration in the algal whitening area.

Effects of Antioxidants on Cell Viability and hGM-CSF Production by Transgenic Nicotiana tabacum Suspension Cultures (형질전환된 Nucotiana tabacum 현탁세포배양에서 항산화제가 세포생존도 및 hGM-CSF 생산에 미치는 영향)

  • Kim Yong Hoon;Lee Sang Yoon;Kim Dong Il
    • KSBB Journal
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    • v.19 no.5
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    • pp.374-380
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    • 2004
  • Production of therapeutic proteins by transgenic plant cell suspension cultures is an attractive system alternative to the other expression system. However, plant cell cultures have shown low expression level of foreign proteins and decreased cell viability by the changes of culture conditions. Therefore, it is necessary to enhance cell viability during the culture period. In this study, a quantitative analysis technique was designed to measure relative cell viability for plant suspension cells which have cell wall and aggregates. It was found that the programmed cell death of plant cells by apoptosis was essentially linked with the apoptotic pathway of animal cells. Therefore, effects of nicotinamide, 3-aminobenzamide and antioxidants on cell viability and apoptosis were examined in transgenic Nicotiana tabacum cells producing hGM-CSF. With those additives, cell viability could be maintained and apoptosis could be redued. In the result, the extracellular production of hGM-CSF could be enhanced 2.5 fold. It was also found that the supplementation of glutathione and ascorbic acid suppressed both the cold stress-induced decrease in cell viability and the increase of total genomic DNA fragmentation.

최근문헌 초록

  • Niino, Niino;Shirata, Kazuto;Oka, Seibi
    • Journal of Sericultural and Entomological Science
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    • v.37 no.2
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    • pp.244-246
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    • 1995
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Effects of Thawing Conditions on the Viability and Acrosomal Morphology of Cryopreserved Boar Semen (동결보존한 돼지정액의 융해조건이 정자의 생존율과 첨체변화에 미치는 효과)

  • 정영호;서경덕;김광식;심금섭;이장희
    • Journal of Embryo Transfer
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    • v.14 no.2
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    • pp.131-137
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    • 1999
  • This experiment was carried out to investigate the effects of osmolarity of thawing diluents, seminal plasma added in thawing diluents on the sperm viability and the effects of thawing temperature, the temparature of the thawing diluents on the sperm viability and acrosomal morphology of boar spermatozoa by the straw method. The result obtained were summarized as follows: 1. The sperm viablilty after thawing of the frozen semen was shown greater in the high osmolarity(392~492mOsm) than low osmolarity(300mOsm) in thawing diluent. The added levels of seminal plasma in thawing diluent did not affect the viability of frozen-thawed boar semen. 2. In terms of thawing temperature, the sperm viability was shown higher in the frozen semen thawed at 5$0^{\circ}C$ for one min. (p<0.01) than those thawed at 2$0^{\circ}C$ or 37$^{\circ}C$ for one min. The sperm viability was not significant at the diluent temparature of 2$0^{\circ}C$or 37$^{\circ}C$ after thawing: but the sperm viability was higher in thawing diluent at 2$0^{\circ}C$ than in that at 37$^{\circ}C$. However, the effects of thawing temperature and diluent solution on normal acrosomal rate were not significant. 3. Cleavage rates of oocytes fertilized with frozen semen were 46.4% and 43.3%, respectively, which were thawed at 5$0^{\circ}C$ for one min. and then diluted in mBTS medium at 2$0^{\circ}C$or 37$^{\circ}C$. To sum up, the sperm viability was shown greater at the high of thawing diluents of frozen boar semen. In terms of thawing conditions, the sperm viability was shown greater, when semen was thawed at a high temperature for a short time and then diluted at the same temperature as that in the straw.

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