• Title/Summary/Keyword: tyrosinase gene

Search Result 17, Processing Time 0.122 seconds

Effect of Chestnut Bark Extracts on Tyrosinase Gene Expression (율피 추출물이 티로시나아제 유전자 발현에 미치는 효과)

  • Chin Jong-Eon;Kim Kwan-Chun
    • Journal of environmental and Sanitary engineering
    • /
    • v.20 no.3
    • /
    • pp.10-16
    • /
    • 2005
  • Chestnut bark extract by methanol repressed the expression of tyrosinase gene of B16 mouse melanoma cell containing tyrosinase promoter. $10{\mu}g/ml{\ell}\;100{\mu}g/m{\ell}$, $1mg/m{\ell}$ of the extract repressed expression of tyrosinase gene about $38\%,\;47\%,\;and\;78\%$, respectively, compared with control. In the MTT assay, the same extract exhibited very low cytotoxicity at $1{\mu}g/m{\ell},\;10{\mu}g/m{\ell},\;100{\mu}g/m{\ell},\;and\;1mg/m{\ell}$, respectively. The fractions of Methylene chloride and ethyl acetate did not showed the repressive effect on the expression of tyrosinase gene, but the fraction of butyl alcohol repressed highly at $10{\mu}g/m{\ell}\;and\;100{\mu}g/m{\ell}$.

The Effects of Areca catechu Extracts on Tyrosinase Gene Expression in B16 Mouse Melanoma Cells (B16 마우스 흑색 종양 세포에서 빈랑(Areca catechu) 추출물이 티로시나아제 유전자에 미치는 효과)

  • Chin, Jong-Eon;Cho, Nam-Chul
    • The Korean Journal of Food And Nutrition
    • /
    • v.20 no.2
    • /
    • pp.240-244
    • /
    • 2007
  • In this study, we found that the methanolic extract of Areca catechu repressed expression of the tyrosinase gene in B16 mouse melanoma cells containing a tyrosinase promoter. Extract concentrations of 100 ${\mu}$g/ml and 500 ${\mu}$g/ml exhibited tyrosinase gene expression rates of aproximately 62% and 48%, respectively, compared to the control. The fraction layers consisting of ethyl acetate, butyl alcohol, and water showed repressive effects on the tyrosinase gene. In particular, the butyl alcohol fraction highly repressed at 100 ${\mu}$g/ml and 500 ${\mu}$g/ml. In the MTT assay, the methanolic extracts exhibited very low cytotoxicities at 1 ${\mu}$g/ml, 10 ${\mu}$g/ml, and 100 ${\mu}$g/ml.

Effect of Clove Extracts on Tyrosinase Gene Expression (정향(Clove) 추출물이 티로시나아제 유전자의 발현에 미치는 효과)

  • Chin, Jong-Eon;Lee, Jeong-Yong;Kim, Kwan-Chun
    • Journal of environmental and Sanitary engineering
    • /
    • v.22 no.1
    • /
    • pp.75-83
    • /
    • 2007
  • Clove extract by methanol increased expression of the tyrosinase gene on B16 mouse melanoma cells containing tyrosinase promoter. $10{\mu}g/mL$ and $100{\mu}g/mL$ of the extract showed expression rate of the tyrosinase gene about 138% and 245%, respectively, compared with control. At $500{\mu}g/mL$, expression rate of the extract was impossible to measurement by high cytotoxicity. The solvent fraction of methylene chloride also exhibited highly expression rate as methanol extract. However, the solvent fractions of butyl alcohol and water showed repressive effect on expression of tyrosinase gene at $500{\mu}g/mL$. In MTT assay, cell survival rate of the extract exhibited similar to expression rate of tyrosinase gene. That is, $10{\mu}g/mL$ and $100{\mu}g/mL$ of the extract showed the cell survival rate about 128% and 187%, respectively.

Detection of 881A→881G Mutation in Tyrosinase Gene and Associations with the Black Ear Coat Color in Rabbits

  • Jiang, Y.L.;Fan, X.Z.;Lu, Z.X.;Tang, H.;Xu, J.-Q.;Du, L.-X.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.15 no.10
    • /
    • pp.1395-1397
    • /
    • 2002
  • The tyrosinase gene was selected as a candidate for uncovering genetic mechanism causing 'black ear' coat color in rabbits. A PCR-SSCP detection method was established for the $881^A{\rightarrow}881^G$ mutation located in the central region of the tyrosinase gene between the CuA and CuB binding region signatures, and this was confirmed by sequencing and alignment. Fully consistent associations between the SNP and 'black ear' coat color were observed by analysis in a "black ear" pedigree and on 61 unrelated individuals. This SNP can serve as a molecular marker for use in "back ear" wool rabbit breeding.

Effects of Scutellaria baicalensis Extracts on Tyrosinase Gene Expression in B16 Melanoma Cells (B16 Melanoma Cell에서 티로시나아제 유전자 발현에 황금(Scutellaria baicalensis) 추출물이 미치는 효과)

  • Cho, Nam-Chul;Bai, Suk;Chin, Jong-Eon
    • The Korean Journal of Food And Nutrition
    • /
    • v.23 no.1
    • /
    • pp.118-123
    • /
    • 2010
  • To estimate the regulatory effects of Scutellaria baicalensis extracts on melanin biosynthesis, we evaluated the regulatory effects of the tyrosinase gene on B16 melanoma cells. The results revealed that methanolic extracts of Scutellaria baicalensis resulted in a high increase in the expression of the tyrosinase gene. Specifically, treatment with extracts at concentrations of $10\;{\mu}g/m{\ell}$ and $100\;{\mu}g/m{\ell}$ resulted in increases in tyrosinase gene expression rates of approximately 231% and 256%, respectively, when compared to the control. Moreover, the solvent fraction layers(methylene chloride, ethyl acetate, butyl alcohol, water) improved the expression of the tyrosinase gene, but to a lesser degree than the methanolic extracts. An MTT assay revealed, that the methanolic extract exhibited very low cytotoxicities at $10\;{\mu}g/m{\ell}$ and $100\;{\mu}g/m{\ell}$. Taken together, the results of this study indicated that the methanolic extracts of Scutellaria baicalensis was a very effective positive regulator of tyrosinase gene expression.

Effect of Houttuynia cordata Extracts on Tyrosinase Gene Expression (어성초(Houttuynia cordata) 추출물이 Tyrosinase유전자 발현에 미치는 효과 -연구노트-)

  • Chin, Jong-Eon;Cho, Nam-Chul
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.34 no.8
    • /
    • pp.1284-1288
    • /
    • 2005
  • The methanol extract of Houttuynia cordata repressed the expression of tyrosinase gene of Bl6 mouse melanoma cell containing tyrosinase promoter. The extract repressed expression of tyrosinase gene by about 13$\%$ and 45$\%$ at 10$\mu$g/mL and 100 $\mu$g/mL, respectively. In the MTT assay, the same extract exhibited very low cytotoxicity at 1$\mu$g/mL, 10$\mu$g/mL, and 100 $\mu$g/mL, respectively. The fractions of ethyl acetate, butyl alcohol, and water did not showed the repressive effect on the expression of tyrosinase gone, but methylene chloride fraction layer repressed highly at 10$\mu$g/mL and 100$\mu$g/mL.

Involvement of Nitric Oxide in UVB-induced pigmentation

  • Horikoshi, Toshio;Sasaki, Minoru;Nakahara, Michio;Uchiwa, Hideyo;Miyachi, Yoshiki
    • Journal of Photoscience
    • /
    • v.9 no.2
    • /
    • pp.205-208
    • /
    • 2002
  • Nitric oxide (NO) is a newly described transmitter involved with cell to cell communication that is generated in biologic tissues by specific types of nitric oxide synthase (NOS), which metabolize L-arginine and molecular oxygen to citrulline and nitric oxide. In the skin. NO has been reported to play an important role in such diseases as psoriasis, atopic dermatitis, and contact dermatitis, as well as act as an important modulator in UVB-induced erythema. Ultraviolet B irradiation to the skin evokes an increase in NO production in the epidermis through two pathways; induction of inducible NOS, mediated by inflammatory cytokines, and elevation of constitutive neuronal NOS activity. In a cell culture system, it has been demonstrated that NO functions as a melanogen after being produced in keratinocytes in response to UVB-irradiation. NO-stimulated melanogenesis in melanocytes is mediated by the cGMP/PKG pathway. In this study, up-regulation of tyrosinase gene expression by NO-stimulation and the involvement of NO in UVB-induced pigmentation were examined. In NO-induced melanogenesis, protein synthesis and tyrosinase activity increased along with an up-regulation of tyrosinase gene expression. In an animal model, UVB-induced pigmentation in skin was suppressed by sequential daily treatments with a specific inhibitor of NOS. Thus, NO plays an important role in UVB-induced pigmentation, where its function as a melanogen is considered to be one of the mechanisms. Together with its role in the development of erythema, NO contributes to the total protective response of skin against UVB-irradiation.

  • PDF

Inhibitory effects of some medicinal plant extracts on the tyrosinase promoter activity on B16 mouse melanoma cells

  • Chin, Jong-Eon;Sun, Heung-Suk;Lee, Kwang-Jae;Choi, Tae-Jin;Ko, Yoo-Seung;Sohn, Hyun-Jung;Kim, Jeong-Joong;Jeon, Byung-Hoon;Blaise Lee, Hwang-Hee
    • Advances in Traditional Medicine
    • /
    • v.1 no.2
    • /
    • pp.6-13
    • /
    • 2000
  • Melanin is specifically produced in melanocytes. The pathway for melanin biosynthesis is mainly controlled by tyrosinase. To estimate the inhibitory effect of melanin biosynthesis from 31 medicinal plants extracts, we tested the inhibitory effects of the tyrosinase promoter on B16 mouse melanoma cells. The result of this study demonstrated that Mori Radicis Cortex and Castena Fractus extracts only in tested medicinal plant extracts have high inhibitory effects on tyrosinase promoters with very low cytotoxicity on B16 mouse melanoma cells. Therefore, extracts of Mori Radicis Cortex and Castena Fractus were evaluated as very effective negative regulators of tyrosinase gene expression.

  • PDF

Characterization of Recombinant Derivatives of pJY711 of Multicopy Streptomyces Plasmid (Multicopy Streptomyces 플라스미드 pJY711의 재조합 유도체의 특성)

  • 염도영;공인수;유주현
    • Korean Journal of Microbiology
    • /
    • v.28 no.1
    • /
    • pp.35-40
    • /
    • 1990
  • The restriction clevage map of multi-copy recombinant plasmid, pJY712(8.1kb), carrying the thiostrepton resistance gene(tsr) was determined. pJY712 had a broad host range in Streptomyces and contained single BglII site for cloning purpose. The plasmid showed the phenomenon of lethal zygosis ($Ltz^{+}$). Transformation frequency of pJY712 was $5.0\times 10^{4}$ transformants per ug plasmid DNA (TFU) in S. lividans. Plasmid pJY713 was constructed by inserting the tyrosinase gene(mel) into the BclI site of pJY712. Recombinant plasmid pJY714 carrying the mel gene was constructed by in vitro deletion of a segment (1.9kb BglII-BclI fragment) from pJY713.

  • PDF

Effect or Onion(Allium cepa L.) Ectract on Tyrosinase Gene Expression (양파(Allium cepa. L)추출물이 Tyosinase 유전자 발현에 미치는 효과)

  • 조남철;윤연희;이혜진;손현정;김양경;최근희;나명석;이황희;진종언
    • The Korean Journal of Food And Nutrition
    • /
    • v.14 no.3
    • /
    • pp.228-232
    • /
    • 2001
  • Melanin 생선에 관여하는 tyrosinase 유전자의 발현을 억제하는 물질을 탐색하고자 tyrosinase promoter를 지닌 B16 mouse melanoma cell.에 양파 methanol 추출물을 처리한 바 양파 methanol추출물은 10.0$\mu\textrm{g}$/$m\ell$, 100.0 $\mu\textrm{g}$/$m\ell$, 1.0$\mu\textrm{g}$/$m\ell$ 농도에서 대조군에 비해서 약 15%, 23%, 57%의 억제효과를 나타냈으며, 세포생존율은 1.0$\mu\textrm{g}$/$m\ell$, 10.0$\mu\textrm{g}$/$m\ell$, 100.0$\mu\textrm{g}$/$m\ell$ 1.0$\mu\textrm{g}$/$m\ell$의 농동에서 약 126%, 92%, 85%, 64%로서 세포독성이 낮게 나타났다. Ethyl acetate butyl alcohol. 그리고 물 용매 분획물은 tyrosinase 유전자의 발현을 억제하는 효과가 없었지만 methylene chloride용해 분획물은 10.0$\mu\textrm{g}$/$m\ell$과 100.0$\mu\textrm{g}$/$m\ell$의 농동에서 약 87%와35%의 발현율을 나타냄으로써 대조군에 비해 크게 억제하였다.

  • PDF