• Title, Summary, Keyword: total plate counts

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Applying the Disinfecting Effects of Vinegar to Raw Vegetables in Foodservice Operations: A Focused Microbiological Quality Evaluation (급식소에서 이용되는 채소류의 식초수 소독의 적용을 위한 연구 - 미생물적 품질 평가를 중심으로 -)

  • Kim, Hyun-Jung;Kim, Heh-Young;Ko, Sung-Hee
    • Korean journal of food and cookery science
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    • v.23 no.4
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    • pp.567-578
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    • 2007
  • This study was designed to provide basic data for the application and practical use of vinegar disinfection for raw vegetable preparation in foodservice operations. The test materials were washed and disinfected by three different methods(tap water washing, chlorine water disinfection, vinegar disinfection) and stored at $3^{\circ}C$ and $10^{\circ}C$ for 7 days. Then, their microbiological qualities were compared. Total plate counts increased over the course of the storage period, and all samples, except in the case of tap water washing, remained under the permitted limit until the 7th day of storage. In particular, vinegar disinfection showed the lowest increase in total plate counts(2.35${\sim}$4.03 log CFU/g). While the total plate counts of chlorine and vinegar disinfection were within the 6.00 limit of microbiological safety, the tap water washing treatment passed the permitted limit. The psychrotrophic counts increased steadily over the course of the storage period, and increased least at $3^{\circ}C$, however, the tap water washing showed the most drastic increase, while vinegar disinfection yielded the smallest increase.

The Conductance Determination of Total, Coliform and Psychrotrophic bacteria Counts in Raw Milk by Using Malthus (Malthus를 이용한 원유(原乳)내의 총균수, 대장균군수, 저온성균수 측정)

  • Nam, Eun-Sook;Chung, Choong-Il;Kang, Kook-Hee;Jeong, Dong-Kwan
    • Korean Journal of Food Science and Technology
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    • v.26 no.6
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    • pp.764-769
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    • 1994
  • This study was performed to obtain fast, consistant and reliable estimation system of bacterial counts of raw milk, which effectively related to the quality of sanitaion and the condition of production at the farm. This study compared regression equation and correlation coefficient relationship between standard plate counts and data of Malthus conductance method for the detection time of total, psychrotrophs, coliform bacterial counts in raw milk. Regression equation (RE) between conductance detection time (Y) and total bacterial log counts (X) was Y=18.27651 - 2.07550X, with correlation coefficient -0.95(n=201). In coliform, RE was Y=9.320848 - 1.15598X with correlation coefficient -0.90 (n=207). Psychrotrophs had the RE of Y=29.96008-3.02487 with correlation coeffecient -0.9 (n=201). This conductance method gave results more quickly and was less labor-intensive than traditional standard plate count method.

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The New Storage Technology: Effect of Far Infrared Ray (FIR) Ceramic Sheet Package on Storage Quality of Pork Loin

  • Lin, Liang-Chuan
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.11
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    • pp.1695-1700
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    • 2003
  • A total of 30 pork loin sections were utilized to evaluate the effects of FIR ceramic sheet in PE and vacuum package on preserving the quality of chilled pork stored at 4 and $0^{\circ}C$. Based on meat color, results indicated that pork loin packaged in ceramic sheet and control treatment showed that the samples of the control treatment tended to darken gradually in comparison with the samples at 0 day, but FIR treatment had few changes. Among the total plate counts of sliced loin in PE and loin in vacuum package under different storage times at 4 and $0^{\circ}C$, results showed that FIR ceramic sheet package treatment had lower total plate counts and significant differences (p<0.05). In VBN value, both treatments tended to rise high with the increasing of storage time, but the FIR treatment was significantly lower (p<0.05) than the control treatment. Its results had a corresponding relationship with the total plate counts. Regarding the drip loss of sliced loin in PE and loin in vacuum package, it showed that FIR ceramic sheet package treatment had lower drip loss and significant differences (p<0.01). These results showed that the use of FIR ceramic sheet package, including PE and vacuum package, is an effective method of maintaining the quality of meat.

Study on Pursuit of Contamination Sources and Establishment of Sanitary Standard from Raw Pork Meat (원료돈육의 오염원 추적 및 위생기준 설정에 관한 연구)

  • 임대석;강희곤;김용곤;김창한
    • Food Science of Animal Resources
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    • v.21 no.2
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    • pp.149-155
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    • 2001
  • The aims of this study were to examine contamination sources and provide the basic data in establishment of sanitary standard for raw pork meat. From \"Random sampling(I)\", initial total plate counts of post-slaughter samples for the group A, B and C were 1.5${\times}10^4$cfu/$textrm{cm}^2$, 5.5${\times}10^5$cfu/$textrm{cm}^2$ and 1.8${\times}10^4$cfu/$textrm{cm}^2$, respectively, and of post-prechilling samples for the group A, B and C were 1.0${\times}10^4$cfu/$textrm{cm}^2$, 4.6${\times}10^5$cfu/$textrm{cm}^2$ and 2.5${\times}10^4$cfu/$textrm{cm}^2$, respectively. Initial total plate counts of post-transportation samples for the group D, E and F did not increased, as did the group A, B and C. From \"Normal sampling(II)\", initial total plate counts of post-slaughter, post-prechilling, post-transportation and post-2 days preservation samples were 7.3${\times}10^4$cfu/$textrm{cm}^2$, 9.6${\times}10^4$cfu/$textrm{cm}^2$, 2.0${\times}10^5$cfu/$textrm{cm}^2$ and 2.5${\times}10^5$cfu/$textrm{cm}^2$, respectively. From \"Clean sampling(III)\", initial total plate counts of post-slaughter, post-prechilling, post-transportation and post-2 days preservation samples were decreased to 7.0${\times}10^2$cfu/$textrm{cm}^2$, 7.5${\times}10^2$cfu/$textrm{cm}^2$, 8.5${\times}10^2$cfu/$textrm{cm}^2$ and 5.5${\times}10^3$cfu/$textrm{cm}^2$, respectively, compared with "Normal sampling(II)". No E. coli O157:H7, Staphylococcus aureus and Salmonella were detected at each sampling step. Consequently, a slaughter method like "Clean sampling(III)" showed a better sanitary effect to low total plate counts of 10$^2$∼10$^3$times, compared with "Normal sampling(II)". The one of contamination sources for raw pork meat was at a slaughtering step, and "Clean sampling" method may be considered as the one of sanitary standards.

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Enumeration and Comparison of Fecal Indicator Bacteria in a Sewage Treatment Plant Using Activated Sludge Process (활성슬러지공정 하수종말처리장의 분원성 지표세균의 농도 및 비교)

  • Lee, Dong-Geun;Sung, Gi-Moon;Jung, Mi-Ra;Park, Seong-Joo
    • Journal of Environmental Health Sciences
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    • v.36 no.2
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    • pp.141-147
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    • 2010
  • Indicator bacteria of fecal pollution were enumerated and compared by various detection methods for influent and final effluent of a sewage treatment plant. Total coliforms were enumerated by four methods including most probable numbers, chromogenic enzyme substrate test, membrane filtration, and plate counts and were about $10^4$ for influent and $10^2{\sim}10^3\;CFU/ml$ for final effluent. Fecal coliforms ranged between $10^3$ and $10^4$ for influent and $10^2\;CFU/ml$ for effluent by chromogenic enzyme substrate test and membrane filtration. Fecal streptococci counts were 1-log less than fecal coliforms counts, $10^2{\sim}10^3$ for influent and $10^1\;CFU/ml$ for effluent. Total coliforms numbers by plate count both in influent and in effluent showed 1-log higher than by the other three methods. Statistical analysis revealed that numbers of total coliforms by plate count in final effluent had the highest average of correlation (r=0.778, p<0.01) compared with those by the other three methods. In addition, total coliforms numbers by plate count showed most significant correlation (r=0.835, p<0.01) with those by chromogenic test which is well-known as its highest recovery efficiency. These results suggest that the plate count would be the optimum detection method for total coliforms in wastewater treatment plants which are the only microbiological standard of final effluent from wastewater treatment plants in the Republic of Korea, considering economic aspects and difficulties in laboratories.

Effect of Gamma Irradiation on the Microflora of Commercial Ready-To-Use (RTU) Salads during Cold Storage

  • Kim, Jang-Ho;Lee, Ju-Woon;Lee, You-Seok;Oh, Sang-Hee;Byun, Myung-Woo
    • Preventive Nutrition and Food Science
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    • v.9 no.3
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    • pp.232-235
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    • 2004
  • Since ready-to-use (RTU) products are not fully cooked, the shelf-life of the product is comparably short and the products are easily spoiled when contaminated with food-borne pathogens. Low-dose gamma irradiation of 0.5, 1, or 2 kGy effectively reduced the total aerobic bacterial counts in 2 Korean manufactured RTU products by 1.63 to 2.95 log CFU/g during cold storage. Irradiation at 2 kGy reduced the psychrotrophic bacterial counts in most of the samples to below the limit of detection (< log CFU/g). Irradiation at 0.5 kGy completely eliminated Escherichia coli from the commercial RTU samples.

Survey of bacterial contamination of chicken meat (도계육에 대한 미생물 오염실태 조사)

  • Kim, Sun-Hee;Na, Ki-Book;Yang, Seung-Min;You, Jae-Yong;Bae, Young-Jae;Choi, Young-Te
    • Korean Journal of Veterinary Service
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    • v.26 no.3
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    • pp.221-225
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    • 2003
  • In order to monitoring the chicken meat hygiene, 165 chicken samples were tested for contamination agents such as the total bacterial counts, coliform bacterial counts and falling bacterial counts. The prevalence of level on number of standard plate count, total bacterial counts in less than $10^3\;cfu/cm^2$, $10^3{\sim}10^4\;cfu/cm^2$ and $10^3-10^5\;cfu/cm^2$ was 30 samples(18.2%), 98 samples(59.4%) and 37 samples(22.4%), respectively. The coliform bacterial counts in less than $10^2\;cfu/cm^2$, $10^2{\sima}10^$\;cfu/cm^2$ and $10^3{\sim}10^4\;cfu/cm^2$ was 7 samples(42.2%), 66 samples(40.0%), 92 samples(58.8%), respectively. The falling bacterial counts in plants was higher 9.1 in summer, 8.4 in fall, 7.2 in spring and 6.7 in winter, in order.

Survey of bacterial contamination of chicken meat (도계육에 대한 미생물 오염실태 조사)

  • 김선희;나기복;양승민;유제용;배영재;최영태
    • Korean Journal of Veterinary Service
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    • v.26 no.1
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    • pp.27-31
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    • 2003
  • In order to monitoring the chicken meat hygiene, 165 chicken samples were tested for contamination agents such as the total bacterial counts, coliform bacterial counts and falling bacterial counts. The prevalence of level on number of standard plate count(SPC), total bacterial counts in less than 10$^3$cfu/$\textrm{cm}^2$, 10$^3$∼10$^4$cfu/$\textrm{cm}^2$ and 10$^4$∼10$\^$5/cfu/$\textrm{cm}^2$ were 30 samples (18.2%), 98 samples(59.4%) and 37 samples(22.4%), respectively. The coliform bacterial counts in less than 10$^2$cfu/$\textrm{cm}^2$, 10$^2$∼10$^3$cfu/$\textrm{cm}^2$ and 10$^3$∼10$^4$cfu/$\textrm{cm}^2$ were 7 samples(4.2%), 66 samples (40.0%), 92 samples(55.8%), respectively. The falling bacterial counts in plants were higher 9.1, in summer 8.4, in fall 7.2 in spring and 6.7 in winter decreased.

Hazard Analysis and Critical Control Points of One-Dish Meal prepared at Korean Restaurants: Naeng-myeun (Cold noodles) and Pi-bim bab (mixed rice) (시판 음식의 조리 단계별 HACCP 설정을 위한 연구(II): 일품요리(냉면, 비빔밥)의 위해요인 분석)

  • Kye, Seung-Hee
    • Journal of the Korean Society of Food Culture
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    • v.10 no.3
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    • pp.167-174
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    • 1995
  • A hazard analysis which included watching operations, measuring temperatures of foods throughout preparation and display, and sampling and testing for microorganisms of total plate counts and coliform bacteria was conducted in various phases of product flow of Naeng-myeun (Cold noodles) and Pi-bim bab (mixed rice) prepared at Korean restaurants. Large numbers of total plate counts were counted from the cooked foods after handling and holding. Ingestion of these foods must be considered high risk. Critical control points identified were, pre-preparation, food handling after cooking, and holding on display in product flow of Nang-Meon and pre-preparation, preparation, and holding on display in product flow of Pi-bim bab. It need for effective quality control of Nang-Meon and Pi-bim bab that training program consist of surveillance, education of the staff, standard operation procedures, forbidding dangerous processes and control of critical points.

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Evaluation of Microbial Quality of the Vegetable Salad Used Dressing Added with Prunus mume Extracts (매실 엑기스 첨가 드레싱을 이용한 채소 샐러드의 미생물적 품질평가)

  • Kim, Heh-Young;Jo, Hyun-A
    • The Korean Journal of Food And Nutrition
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    • v.23 no.2
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    • pp.240-246
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    • 2010
  • This study aimed to determine microbiological quality on vegetable salad used dressing added with Prunus mume extracts. For this study, Dressing were blended with Prunus mume extracts to different concentrations of 0, 10, and 20%. Microbiological effects of vegetable salad used dressing added with Prunus mume extracts were assessed during production process by measuring process time, temperature, pH and Aw and determining total plate counts and coliforms. Effects of vegetable salad used dressing added with Prunus mume extracts on total plate counts and coliforms were observed during holding at 3, $25{\pm}1^{\circ}C$ for 12 hours. Dressing added with Prunus mume extracts improved the microbiological quality and showed antibacterial properties when they are added to vegetable salad.