• Title, Summary, Keyword: total SOD

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Optimization of Extraction Conditions of Pleurotus cornucopiae by Response Surface Methodology (반응표면분석에 의한 노랑느타리버섯 추출물의 추출조건 최적화)

  • Lee, Hye-Jin;Do, Jeong-Ryong;Chung, Min-Yu;Kim, Hyun-Ku
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.10
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    • pp.1565-1570
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    • 2014
  • Response surface methodology (RSM) was used to optimize extraction conditions of Pleurotus cornocopiae. Coefficients of determination (R2) for dependent variables ranged from 0.86 at 0.91. Maximum extraction yield was 40.81% under the following conditions: microwave power of 60.08 watts, ethanolic concentration of 12.33%, and extraction time of 6.86 min. Maximum extraction electron donating ability was 35.72% at 44.13 watts, 19.30%, and 4.21 min. Maximum extraction superoxide dismutase (SOD)-like activity was 34.87% at 114.01 watts, 65.88%, and 1.56 min. Maximum extraction total polyphenol content was 31.77 mg/g at 50.52 watts, 23.00% and 2.90 min. Based on the superimposition of four dimensional RSM with respect to extraction yield, electron donating ability, SOD-like activity, and total polyphenol content obtained under various extraction conditions, the optimum ranges of extraction conditions were as follows: microwave power of 71.48~92.84 watts, ethanol concentration of 55.01~71.66%, and extraction time of 3~9 min.

An anti-Dxidative Effect of Cheongeumyeonsudan of Rats Induced Aging by D-Galactose (천금연수단(千金延壽丹)이 노화 유발된 철쥐의 항산화효능에 미치는 영향)

  • Song, Kyoung-Hye;Seo, Jung-Min;Beak, Jung-Han
    • The Journal of Pediatrics of Korean Medicine
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    • v.20 no.1
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    • pp.31-47
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    • 2006
  • Objectives : The purpose of this study is to find out the effect of antioxidation related to aging of Cheongeumyeonsudan which is written on Dongui-bogam experimentally. Method : 14 weeks aged SD albino rats were separated into uncontrolled group, controlled group and CGY group. As controlled and CGY groups were induced aging by subcutaneous injection of D-galactose, at the same time we administered the extract of Cheongeumyeonsudan to CGY group for 6 weeks. After then we drew blood from each group, and took measurements; the activity of SOD, GSH-px, catalase in erythrocytes, TBARS value, concentration of total lipid, tryglycende, total cholesterol, HDL-cholesterol in blood plasma. Results : The activities of SOD, GSH-px in erythrocytes were significantly increased in the CGY group compared with control group. The activity of catalase showed a tendency to increase, but it was nor remarkable. The concentration of total lipid, the values of TBARS and total cholesterol was significantly decreased in the CGY group compared with control group, and the concentration of plasma HDL-cholesterol was not remarkable. The concentration of tryglycende in plasma showed a tendency to decreased. Conclusions : it is suggested that Cheongeumyeonsudan decreased the activities of free radical, the concentration of lipid in plasma and generate enzyme which form lipid peroxide.

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Changes of Photosynthetic Pigment Contents and SOD Activity in the Leaves of Four Tree Species Exposed to SO2 (SO2 노출된 4개 수종의 엽내 광색소 함량 및 SOD 활성 변화)

  • 이재천;한심희;권기원;우수영;최정호
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.5 no.1
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    • pp.18-23
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    • 2003
  • This study was conducted to compare physiological responses of Pinus densiflora, Populus ${\times}$ tomentiglandulosa, Quercus acutissima and Eleutherococcus sessiliflorus exposed to SO$_2$, by measuring photosynthetic pigment contents and SOD activity. Four woody plants were exposed to relatively high SO$_2$ concentration (500 ppb, 800 ppb) for 8h day$^{-1}$ for 7 days in a chamber. Photosynthetic pigment contents in the leaves of four species decreased with increase of SO$_2$ concentration; also chlorophyll a, chlorophyll b and total carotenoid content were significantly different among tree species and treatments. The ratio of chlorophyll b to chlorophyll a of E. sessiliflorus and Q. acutissima increased for 500 ppb treatment but decreased at 800 ppb. This result showed that chlorophyll a was destroyed by 500 ppb SO$_2$ and chlorophyll b by 800 ppb SO$_2$. Therefore, the sensitivity of chlorophyll a to SO$_2$ may be higher than that of chlorophyll b. SOD activity differed significantly between species and treatments. SOD activity of E. sessiliflorus and Q. acutissima increased at 500 ppb but decreased at 800 ppb, but P. densiflora and P ${\times}$tomentiglandulosa maintained high SOD activity at both 500 ppb and 800 ppb. Based on the photosynthetic pigment contents and SOD activity in the leaves of four tree species, the tolerance of P. ${\times}$ tomentiglandulosa to SO$_2$ was the highest of four tree species.

A Study on SOD Activity and Serum Antioxidant Mineral Concentrations in Obese Adolescents (비만 청소년의 SOD 활성도 및 혈청 항산화무기질 농도에 관한 연구)

  • Lee, Da-Hong
    • Journal of Nutrition and Health
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    • v.40 no.1
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    • pp.41-48
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    • 2007
  • The purpose of this study was to investigate the relationship between obesity and, erythrocyte SOD (superoxide dismutase) activity and serum antioxidant mineral (Fe, Zn, Cu, Mn and Se) concentrations of adolescents. Subjects were assigned to one of two groups such as obese ($BMI{\geq}25$, 32 boys, 24 girls) and normal group (18.5 < BMI < 23, 27 boys, 30 girls) Subjects were evaluated based on anthropometric measurements, 24-hr dietary recalls and blood analysis. The mean age of the total subjects was 13.8 years. The mean weight (p < 0.001), BMI (p < 0.001) and body fat (p < 0.001) of obese were higher than those of normal group. There was no significant difference in nutrient intake between obese and normal groups. SOD activity of obese group was not significantly different from normal groups, in both males and females. However, in the males, serum Cu concentration of obese were significantly lower than those of normal group. In the females, Serum Mn concentration of obese were significantly lower then those of normal group. In the correlation analysis, BMI of the subjects had significantly negative correlations with serum Cu, Zn and Mn. To summarize the results, increase of obesity may lead to decrease of serum antioxidant minerals such as Cu, Zn and Mn.

Biological Activities of Maca (Lepidium meyenii) Extracts (마카 추출액의 생리활성 효과)

  • Kwon, Yun-Suk;Jeon, In-Sook;Hwang, Jin-Hyeon;Lim, Dong-Min;Kang, Yong-Soo;Chung, Hai-Jung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.7
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    • pp.817-823
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    • 2009
  • This study was conducted to determine the optimal extraction conditions for maca by comparing the yields, total polyphenol contents, superoxide dismutase (SOD)-like activity and the nitrite scavenging ability. The proximate composition analysis showed 6.57% moisture, 12.83% crude protein, 1.05% crude fat, 4.80% ash and 74.75% carbohydrate. Maca was extracted with 7 different solvents (water, methanol, ethanol, acetone, ethyl acetate, chloroform and hexane) and the extracts were tested for biological activities. The extraction yields of water, methanol and ethanol extracts were 46.2%, 21.4% and 16.8%, respectively. Acetone, ethyl acetate, chloroform and hexane exhibited very low extraction yield, ranging from 0.2 to 1.0%. Total polyphenol contents and the nitrite scavenging ability were the highest in water extract. Electron donating ability and the SOD-like activity were the highest in methanol extract. When water extract was drawn out at different extraction temperatures (30, 70, $100^{\circ}C$) and time (1, 3, 5 hr), the improved biological activities (total polyphenol contents, electron donating ability, SOD-like activity and nitrite scavenging ability) were found in extracts treated at $100^{\circ}C$ for 3 or 5 hrs.

Molecular Cloning and High-Level Expression of Human Cytoplasmic Superoxide Dismutase Gene in Escherichia coli (사람의 세포질 Superoxide Dismutase 유전자의 클로닝과 대장균내에서의 대량발현에 관한 연구)

  • 이우길;김영호;양중익;노현모
    • Korean Journal of Microbiology
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    • v.28 no.2
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    • pp.91-97
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    • 1990
  • Complementary DNA (cDNA) coding for human cytoplasmic superoxide dismutase (SOD1) (superoxide: superoxide oxidoreductase E.C.1.15.1.1) was isolated from human liver cDNA library of $\lambda$gt11 by in situ plaque hybridization. The insery cDNA gas the 5' untranslational region (UTR) and 3'UTR of SOD1 gene. Polymerase Chain Reaction (PCR) method was used fro subcloning of SOD1 structural gene. Using synthetic sense strand primer (24mer) containing a start codon and antisense strand primer (24mer), SOD1 structural gene was selectively amplified. Amplified DNA was directly cloned into the HincII site of pUC19 plasmid. Insery cDNA was subcloned into M13 mp19 and sequenced by dideowy chain termination method with Sequenase. The nucleotide sequence of insert cDNA had an open reading frame (ORF) coding for 153 amino acid residues. The structural gene of cytoplasmic SOD was placed under the control of bacteriophage $\lambda P_{L}$ regulatory sequences, generating a highly efficient expression plasmid. The production of human SOD1 in E. coli cells was about 7% of total cellular proteins and recombinant human SOD1 possessed its own enzymatic acitivity.

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Effects of Iksugangjiryung on Hyperlipidemia in Rats (익수강지령(益壽降脂靈)이 흰쥐의 고지혈증 병태에 미치는 영향)

  • No, Hyun-Suk;Kim, Jeung-Beum
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.5
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    • pp.1283-1292
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    • 2008
  • This study was designed to investigate the hyperlipidemia-healing effect of water extract from Iksukangjiryung, it was performed on the hyperlipidemia of rats induced by Triton WR-1339 and high cholesterol diet. We prepared two types of hyperlipidemia model in rats induced by Triton WR-1339 and high cholesterol diet. The amount of total cholesterol, HDL-cholesterol, LDL-cholesterol, triglyceride, lipid peroxide, glutathione and the activity of superoxide dismutase(SOD) were measured. The water extract from Iksukangjiryung showed significant decreasing effects on total cholesterol, LDL- cholesterol, triglyceride, lipid peroxide in the both model. And it showed significant increasing effects on the activity of SOD in both model and amount of glutathione in Triton WR-1339 model. These results suggest that water extract from Iksukangjiryung has healing efficacy on hyperlipidemia induced by Triton WR-1339 and high cholesterol diet.

Effect of Korean Native Plant Diet on Lipid Metabolism, Antioxidative Capacity and Cadmium Detoxification in Rats (한국 고유의 산채류 첨가 식이가 흰쥐의 지방 대사 및 항산화능과 Cadmium 제독에 미치는 영향)

  • 박진아
    • Journal of Nutrition and Health
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    • v.32 no.4
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    • pp.353-368
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    • 1999
  • This study was performed to investigate the effects of Korean native plant diets on lipid and cadmium (Cd) metabolisms and also antioxidative enzyme activities in rats. Seventy male Sprague-Dawley rats weighing 210.3$\pm$2.7g were blocked into ten groups according to body weight. Rats were raised for four weeks with diets containing 0 or 0.04%(w/w) cadmium chloride and 5%(w/w) plant powder-Ssook(Artemisia princeps var. orientalis HARA), Chamchwi(Aster scaber THUNB), Gomchwi(Lingularia fischeri TUBCZ). or Solbeerum (Portulaca oleracea LINNE). Food intake, weight gain, food efficiency ratio, and weights of liver, kidney, spleen and epididymal fat were significantly lower in Cd-exposed groups, food efficiency ratio, and weights of liver, kidney, spleen and epididymal fat were significanlty lower in Cd-exposed groups. Plasma total lipid level, liver total lipid, cholesterol and triglycerde concentrations, and fecal total lipid, cholesterol and triglyceride excretions were decreased by Cd administration. Total lipid and triglyceride concentration in plasma and liver were lower in Chamchwi groups together with increased fecal total lipid and triglyceride excretions. Cholesterol concentration in plasma and liver were lower in Gomchwi groups with increased fecal cholesterol excretions. Activities of red-blood-cell superoxide dismutase(SOD), and liver catalase, SOD and glutathione peroxidase (GSH-px) were decreased significantly by Cd administration. Thiobarbituric acid reactive substance(TBARS) concentrations of plasma and liver were increased by Cd administration. Thiobarbituric acid reactive substance(TBARS) concentration of plasma and liver were increased by Cd administration. Administration of plant diets decreased plasma and liver catalase, SOD and GSH-px activities in Cd-free diet groups and increased them in Cd-administered groups. Plasma and liver TBARS concentrations were decreased in animals fed plant diets, with Chamchwi showing the most effective antioxidative capacity. The concentration of Cd accumulated in blood and tissue decreased in Cd-exposed groups fed plant diets. Liver Cd concentration were lower in the Chamchwi and Gomchwi groups, and kidney Cd levels were lower in the Ssook and Chamchwi groups. Among them, Chamchwi was the most effective in Cd detoxification. In conclusion, Chamchwi and Gomchwi were effective in lowering tissue lipid levels, with Chamchwi having the greatest antioxidative and detoxifying effects.

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Effect of Tota1 Saponin from Red Ginseng on Acvtivities of Antioxidant Enzymes in Pregnant Rats (홍삼 사포닌이 수태중인 흰쥐의 항산화 효소활성에 미치는 영향)

  • Song, Yong-Bum;Kwak, Yi-Seong;Park, Ki-Hyun;Chang, Sung-Keun
    • Journal of Ginseng Research
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    • v.26 no.3
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    • pp.139-144
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    • 2002
  • Pregnancy is a physiological state accompained by a high energy demand of many bodily functions and an increased oxygen requirement. Because of the increased intake and utilization of oxygen, increased levels of oxidative stress would be expected. So we observed the activities of the hepatic antioxidant enzymes from rat treated with total saponin from the red ginseng against free raicals produced in pregnant rats. The activity of superoxide dismutase (SOD) in the control group was slightly decreased during pregnancy, and SOD activity in total saponin treated group was not observed any siginificant change compared with the control group. The activities of glutathione peroxidase (GPX), glutathione reductase (GRD) and catalase in the control group have shown the decreasing tendency during pregnancy, whereas the activities of GRD and catalase in total saponin treated group showed significant increased tendency compared with the control group. GPX activity in total saponin treated group was slightly decreased tendnency compared with the control group. The activity of glutathione-S-transferase (GST) in the control group was increased to keep the state of homaeostasis tendency in pregnant rats. On the other hand, the activity of GST after total saponin treatment was increased than control group. Activity of all enzymes in the control group and total saponin treated group recovered the normal level after delivery of rats. In spite of the physiological changes in vivo, the inflaunce of total saponin on activaties of hepatic antioxidant enzyme in pregnant rats seems to be regulated the biological homeostatic adaptation mechanism which protects the maternal liver aganist oxygen induced toxicity

Effects of Red Ginseng Component on the Antioxidative Enzymes Activities and Lipid Peroxidation in the Liver of Mice (홍삼 추출물 투여가 생쥐간에서 항산화 효소 활성과 지질과산화에 미치는 효과)

  • Sung, Kum-Soo;Chun, Chul;Kwon, Young-Hun;Kim, Kyon-Hyun;Chang, Che-Chul
    • Journal of Ginseng Research
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    • v.24 no.1
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    • pp.29-34
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    • 2000
  • The effects of each component (water extracts, alcohol extracts, lipophillic extracts, total saponin, panaxadiol, panaxatriol) of red ginseng on the antioxidative enzyme activities were investigated in the liver in order to screen antioxidative components of red ginseng. 20∼25g ICR mouse which were pretreated with 50 mg/kg body weight of red ginseng component for 15 days. The ability of red ginseng component to protect against oxidative damage to the mouse liver was examined by determining the level of lipid peroxidation (MDA), hydroperoxide (H$_2$O$_2$) and the activities of superoxide dismutase (SOD), catalase. The hepatic total-SOD activity was highest in lipophilic extracts group and panaxadiol group next (p<0.01). The content of hepatic hydroperoxide was lowest in the order of panaxatriol group and alcohol extracts group (p < 0.01). The hepatic catalase activity in the liver was highest in order of lipophillic extracts group (p <0.01) and total saponin group (p<0.05). Finally the lipid peroxidation (MDA) level was lowest in lipophillic extracts group, alcohol extracts group and panaxadiol next (p <0.01). In conclusion, the order of effectiveness of antioxidants was to be lipophillic extracts>panaxadiol >total saponins.

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