• Title, Summary, Keyword: total SOD

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Antioxidant Activity and Total Phenolic Compounds off P. eryngii spp. Extracts (버섯(P. eryngii spp.) 추출물의 항산화 활성과 총 페놀함량)

  • Chi Hee-Youn;Kim Kyu-Hyun;Kong Won-Sik;Kim Sun-Lim;Kim Jin-Ae;Chung Ill-Min;Kim Jung-Tae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.spc1
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    • pp.216-219
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    • 2005
  • This objective of this study was to determined the activity of antioxidants of DPPH, SOD and total phenolic compounds in mushroom(P. eryngii spp.) extract. The antioxidant acitivity using DPPH showed that strain No. 2007$(31.78\%)$ and strain No.2014$(27.45\%)$ were the highest DPPH inhibition. Among the thirteen mushroom examined, strain No. 2014$(58.30\%)$ and strain No. 2010$(57.19\%)$ had the highest SOD activity. The highest total phenol content were in strain No.2013$(1465.03{\mu}g/g)$ and strain No.2005$(1401.09{\mu}g/g)$. Our study suggest that it may be possible to improve mushroom with high natural antioxidant.

Superoxide Dismutase Activity in Suspension Cultured Cells of Tomato (Lycopersicon esculentum Mill) (토마토(Lycopersicon esculentum Mill) 현탁배양세포에서 Superoxide Dismutase 활성)

  • 유순희;허경혜;권석윤;이행순;방재욱;곽상수
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.1
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    • pp.57-61
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    • 1997
  • We investigated changes in the superoxide dismutase (SOD) activity and SOD isoenzyme pattern in suspension cultures of tomato (Lycopersicon esculentum), which were compared with those of intact tomato plants. two grams (fr wt) of cells subcultured at 15-day intervals were inoculated into 50 mL MS medium containing l mg/L 2,4-D and 30 g/L sucrose in a 300 mL flask and maintained at $25^{\circ}C$ in the dark (100 rpm). The cell growth reached a maximum at 20 days after subculture (DAS), followed by a rapid decrease with further cultures. The cell colour changed from white to black from 23 DAS. The intracellular SOD activity (units/g cell dry wt) was significantly increased from 23 DAS and reached a maximum at 28 DAS (52,400 units), followed by a decrease with further cultures, whereas the extracellular SOD activity showed a maximum at 25 DAS (27,800 units/50 mL medium). The total SOD activity per flask showed a maximum at 25 DAS (35,700 units), in which the extracellular SOD activity occupied about 75%. The tomato cultured cells had four SOD isoenzymes and their patterns were well correlated with SOD activity without a qualitative change during the cell cultures. The intact tomato plants had an additional CuZnSOD isoenzyme, showing the different isoenzyme patterns from cultured cells.

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Effect of Oxygen Concentrations with Superoxide Dismutase on In Vitro Maturation of Porcine Follicular Oocytes and In Vitro Development of Porcine IVM/IVF Embryos (산소농도 및 Superoxide Dismutase가 돼지 난포란의 체외성숙과 배발달에 미치는 영향)

  • 한만희;이규승
    • Journal of Embryo Transfer
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    • v.16 no.3
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    • pp.173-182
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    • 2001
  • The present study was carried out to examine the effect of superoxide dismutase (SOD) on in vitro maturation (IVM) of porcine follicular oocytes and oxygen concentration with SOD on in vitro development (IVD) of porcine IVM/IVF embryos. The results were summarized as follows : 1. The rates of nuclear maturation, penetrated oocytes, polyspermic oocytes and mean numbers of the penetrated sperm were not different in the NCSU-23 maturation media with 0, 100, 500 and 1,000 units/ml SOD. However. the pronucleus formation rates were significantly lower in oocytes matured with addition groups than those of no addition groups of SOD (P>0.05). 2. The rates of blastocyst formation and total cell numbers of blastocyst at day 7 after in vitro fertilization were significantly lower in addition groups than those of the no addition groups of SOD (P>0.05). 3. The rates of blastocyst formation at day 7 after in vitro fertilization were higher in the NCSU-23 culture medium with 100 units/ml SOD than in those cultured with 0, 500 and 1,000 units/ml SOD under the 5% and 20% $O_2$concentrations. However, no differences was found in the total cell numbers of blastocyst among the treatments. In conclusion, these results suggested that the addition of SOD was not adequate for porcine oocyte maturation and further development, also the rates of blastocyst formation and total cell numbers of blastocyst at day 7 of porcine IVM/IVF embryos were not different in the NCSU-23 culture medium under the 5% and 20% $O_2$concentrations.

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Effects of Gal geun (Puerariae Radix) on lowering lipid and antioxidant

  • Lee, Eun;Kang, Jin-Ho
    • Korean Journal of Plant Resources
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    • v.21 no.6
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    • pp.457-461
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    • 2008
  • Effects of Gal geun (Puerariae Radix) EtOH ext. on lipid lowering and antioxidant were investigated in hyperlipidemic rat. Concentration of FFA and triglyceride in plasma showed a tendency to decrease in Gal geun ext. groups. Concentration of plasma total cholesterol and LDL-cholesterol decreased in Gal geun ext. groups. However the concentration of HDL-cholesterol showed no significantly different in all treatment groups. Concentration of liver total cholesterol and triglyceride showed a tendence to decrease in Gal geun ext. groups. Concentration of plasma and liver TBARS showed a low values in Gal geun ext. groups. The values of GSH-Px and SOD activity showed no significantly different among all the treatment groups. However the values of SOD and CAT activity showed a high value in the Gal geun ext. groups.

Effects of Cadmium on Superoxide Radical Superoxide Dismutase, Catalase and ATPase Activit in liver, Kidney and Testicle of Rats in Vitro and in Vivo (시험관내 및 생체내로 투여한 카드뮴이 랏트의 간, 신 및 고환조직 내의 Superoxide Radical, Superoxide Dismutase, Catalase 및 ATPase 활성도에 미치는 영향)

  • Kim, Sung-Moo;Chung, Kyou-Chull
    • Journal of Preventive Medicine and Public Health
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    • v.23 no.4
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    • pp.371-390
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    • 1990
  • Production of free radicals of superoxide anion in tissues by cadmium, activities of superoxide dismutase and catalase to protect tissue damages caused by the free radicals and ATPase that plays an important role in energy metabolism at cellular level were investigated. Experiments in vivo were conducted with liver, kidney and testicle tissue homogenates of rats adding $0.05{\sim}0.50mM$ cadmium chloride, and in vivo experiments administering single dose of 5 mg of cadmium/kg of body weight in 0.1% cadmium chloride solution intraperitoneally 48 hours prior to evisceration. Production of superoxide radicals in liver and testicle increased with addition of cadmium in vitro, but not in kidney. In vivo experiments, however, superoxide radicals slightly increased in liver and kidney but not in testicle. Superoxide dismutase (Cu, Zn-SOD and Mn-SOD), catalase and ATPase (total, $Mg^{++}-\;&\;Na^+,\;K^+-$) activity decreased in the presence of cadimium in dose dependent manner. Reduction of these enzyme activities varied not only with dosage of cadmium but also with type of tissue and between in vitro and in vivo experiment.

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Activity and Isozyme Profile of Antioxidative Enzymes at Booting Stage of Rice Treated with Cold Water

  • Kim Ki-Young;Kim Bo-Kyeong;Shin Mun-Sik;Choung Jin-Il;Ko Jae-Kweon;Kim Jung-Kon;Lim Jung-Hyun;Yun Song-Joon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.4
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    • pp.289-294
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    • 2004
  • This study was carried out to investigate the antioxidative enzymes and isozymes between chilling-tolerant and -susceptible varieties at the booting stage under cold water stress $(13^{\circ}C)$ in japonica rice. Total SOD, CAT, POX, and GR activities on the basis of protein were found to be important factors to defend cold water stress. Especially, SOD and CAT activities showed distinctive differences between chilling-tolerant and -susceptible varieties. Chilling-tolerant varieties were higher than chilling-susceptible varieties for SOD and CAT activities. One of eight isozyme bands for SOD was a inducible isoform. Three isozymes for CAT and one isozyme for POX were closely correlated with defense to cold water stress. Total GR activities except Stejaree 45 on the basis fresh weight and POX were increased by cold water stress, but there was no difference between chilling-tolerant and -sus­ceptible varieties.

Antihyperlipidemic and Antioxidant Effects of Poncirus trifoliata (탱자 열매 추출액의 항산화 및 지질저하효과)

  • Lee Eun
    • Korean Journal of Plant Resources
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    • v.19 no.2
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    • pp.273-276
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    • 2006
  • Lipid-lowering and antioxidant effects of Poncirus trifoliata extract were investigated in hyperlipidemic rat. Concentrations of FFA and triglyceride in plasma were decreased in Poncirus trifoliata extract treatment groups. Plasma total cholesterol and LDL-cholesterol were lower in Poncirus trifoliata extract treatment groups. However HDL-cholesterol showed no significant difference in all treatment groups. Concentrations of liver total cholesterol and triglyceride were decreased in Poncirus trifoliata extract treatment groups. Concentrations of plasma and liver TBARS showed a low values in Poncirus trifoliata extract treatment groups. The values of GSH-Px and CAT activities were increased in the Poncirus trifoliata extract treatment groups. However the values of SOD activity showed no significant difference in all treatment groups.

오미자(Schizandra Chinesis Baillon)추출물의 분획별 항상화활성 효과

  • Kim, Ji-Eun;Hwang, Hyeon-Ik;Lee, In-Sun;Mun, Hye-Yeon
    • 한국생물공학회:학술대회논문집
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    • pp.317-320
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    • 2002
  • To search for antioxidant compounds from plant resources, ethanol extracts of 10 plants species were investigated using DPPH method and SOD activity by NBT method. The highest activity was shown in the ethanol extracts of Schizandra Chinesis Baillon. And than, antioxidant effects and total polyphenol concentration were investigated in solvent fraction of ethanol extracts from Schizandra Chinesis Baillon. The DPPH inhibitory activity and SOD activity of ethyl acetate fraction (DPPH 51.6%, SOD 66.2%) and butanol fraction(DPPH 60.7%, SOD 67.4%) showed strong biological activities.

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Effects of geagibokrounghwan on superoxide dismutase gene expression in experimental mouse fed cholesterol (계지복령환이 Cholesterol 식이 생쥐의 SOD 유전자발현능에 미치는 영향)

  • Hong Yun-Pyo;Ro Sung-Hyun
    • Herbal Formula Science
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    • v.6 no.1
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    • pp.215-226
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    • 1998
  • Geagibokrounghwan (桂技茯笭湯) has long been used to cure human diseases such as vascular and blood disorders. However, it is still unkown on its action mechanism, physiolosical and biochemical meaning. Thus, many attempts were tried to show the scientific background covering the above mentioned mechanism. The effect of Geagibokrounghwan, which was known to date, as follow; effective circulation of body blood system, proliferation of leucocytes and antioxidative action. In this study, we have applied the Geagibokrounghwan administration and feed to mouse, to see effects on the expression of superoxide dismutase(SOD) mRNA as antioxidative agent and oxygen radical scavenger. Total RNAs includingmRNA have been isolated from liver and white blood cells after mice were fed with cholesterol in high dose. Also, in a separate group, the cholesterol-administrated mice were fed with Geagibokrounghwan to see the effects on SOD transcription. and then reverse transcriptase-polymerase chain reaction (RT-PCR) usion each primer set (SOD-F;GATGAAAGCGGTGT-3'; SOD-R; 5'-CCTGTGGAGTGATT-3') were performed to trace theamounts of mRNA. SOD mRNA was specifically expressed in Geagibokrounghwan-fed mice at 2 weeks after treatment, however, gradually reduced after 4 weeks. These results indicate that Geagibokrounghwan is highly applicable in treatment of the above mentioned human diseases.

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Antioxidatibe Mechanism of Total Saponin of Red Ginseng (홍삼 총 사포닌의 항산화작용 기전)

  • Kim, Jung-Sun;Nam, Kyu;Shim, Kyung-Hee;Kim, Kyu-Won;Im, Kwang-Sik;Chung, Hae-Young
    • Journal of Life Science
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    • v.6 no.1
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    • pp.48-55
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    • 1996
  • Oxygen free radicals are highly reactive molecules with unpaired electrons, which are produced with in aerobic cells in the course of normal metabolic events. Normally, aerobic cells are protected from the damage of free radicals by antioxidative enzymes such as superoxide dismutase (SOD), catalase, glutathione (GSH) peroxidase, GSH S-transferase and GSH reductase which scabvenge free radicals as well as nonenzymatic antioxidants such as ceruloplasmin, albumin and nontioxidants in order to elucidate antioxidative mechanisms of red ginseng. The treatment with total saponin of red ginseng significantly devreased the contents of malondialdehyde and total free radicals in the liver. On the other hand, total saponin of red ginseng significantly increased the activities of SOD, catalase and GSH reductase and nonprotein-SH level. These results suggest that total saponin of red ginseng exerts an antioxidative effect by increasing endogenous antioxidants.

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