• Title, Summary, Keyword: tomato fruit

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The use of SlAdh2 promoter as a novel fruit-specific promoter in transgenic tomato

  • Chung, Mi-Young;Naing, Aung Htay;Vrebalov, Julia;Shanmugam, Ashokraj;Lee, Do-Jin;Park, In Hwan;Kim, Chang Kil;Giovannon, James
    • Journal of Plant Biotechnology
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    • v.47 no.2
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    • pp.172-178
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    • 2020
  • Fruit-specific promoters play an important role in the improvement of traits, such as fruit quality through genetic engineering. In tomato, the development of fruit-specific promoters was previously reported, but less attention has been paid to the promoters involved in the fruit development stage. In this study, we characterized the gene expression patterns of tomato alcohol dehydrogenase 2 (SlAdh2) in various tissues of wild-type tomato (cv. Ailsa Craig). Our findings revealed that SlAdh2 expression levels were higher in the developing fruit than in the leaves, stems, and flowers. The ProSlAdh2 region, which is expressed at different stages of fruit development, was isolated from tomato genomic DNA. Following this, it was fused with a β-glucuronidase reporter gene (GUS) and introduced into wild-type tomato using Agrobacterium-mediated transformation to evaluate promoter activity in the various tissues of transgenic tomato. The ProSlAdh2:GUS promoter exhibited strong activity in the fruit and weak activity in the stems, but displayed undetectable activity in the leaves and flowers. Interestingly, the promoter was active from the appearance of the green fruit (1 cm in size) to the well-ripened stage in transgenic tomatoes, indicating its suitability for transgene expression during fruit development and ripening. Thus, our findings suggest that ProSlAdh2 may serve as a potential fruit-specific promoter for genetic-based improvement of tomato fruit quality.

Changes in flavor-relevant compounds during vine ripening of tomato fruit and their relationship with ethylene production

  • Wang, Libin;Luo, Weiqi;Sun, Xiuxiu;Qian, Chunlu
    • Horticulture, Environment, and Biotechnology : HEB
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    • v.59 no.6
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    • pp.787-804
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    • 2018
  • Flavor quality is import for determining consumer perception and acceptance of tomato products. In this study, 'Fendou' tomato fruit were harvested at six ripening stages and sampled to investigate the development of flavor-relevant compounds during vine ripening. Results showed that upon the initiation of ripening there was an increase in respiration rate and concomitant ethylene evolution that was associated with increased membrane permeability. In accordance with these physiological changes, flavor-relevant compounds demonstrated different expression patterns as fruit ripened, which contributed to 'red-ripe' flavor characteristics of red-ripe fruit. Based on correlation analysis between ethylene evolution and the flavor-relevant compounds during 'Fendou' tomato ripening and the other researchers' reports, the activation of System 2-dependent autocatalytic ethylene production plays an important role in the development of most flavor-relevant compounds during tomato vine ripening. Overall, our results suggested that most flavor-relevant compounds that accumulated the most during tomato fruit ripening at red stage could be under ethylene regulation and were among the most important contributors to the 'red-ripe' flavor. Due to the development of these compounds, the flavor quality at late ripening stages is different from that of fruit at early ripening stages.

Phenolic Components and Antioxidant Capacity of Some Selected Fruit Juices and Fermented Grape Juices (과일즙 및 발효 포도즙의 페놀성 화합물 함량과 항산화 활성)

  • Nam Jin-Hee;Joo Kwang-Jee
    • Journal of the East Asian Society of Dietary Life
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    • v.14 no.5
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    • pp.501-507
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    • 2004
  • Some selected fruit juices and fermented red grape juices were investigated to determine the phenolic components by the Folin-Ciocalteu method and antioxidant capacity using α, α-diphenyl-β-picrylhydrazyl(DPPH) method. Commercial red grape beverages and red wines were also analyzed as control groups. In the juice, kiwi fruit had the highest phenolic components followed by orange(summer), red grape(Cambell Early:Yonng-Chun), mandarin orange(autumn), apple(Hong-Ok), cherry tomato, tomato and water melon. Whereas, on the antioxidant efficiency, tomato showed the highest free radical scavenging effect followed by orange(summer), cherry tomato, mandarin orange(autumn), apple(Aori) red grape(Cambell Early:Sung-Ju), kiwi fruit and water melon. The amount of pheonlic components of red wine was 2 times of that of fermented sugar added grape juice, however, the antioxidant efficiency of fermented sugar added grape juice was almost the same as that of red wine. It was found that no clear relationship could be shown between the content of phenolic component and antioxidant capacity of fruit juices and fermented red grape juices. The fruit juices from tomato, orange, cherry tomato, mandarin orange, red grape and fermented sugar added red grape juice showed high free radical scavenging effect and should be regarded as a valuable source of antioxidant.

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Multi-Operation Robot For Fruit Production

  • Kondo, Naoshi;Monta, Mitsuji;Shibano, Yasunori
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • pp.621-631
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    • 1996
  • It is said that robot can be used for multi-purpose use by changing end effector or/and visual sensor with its software. In this study, it was investigated what multi-purpose robot for fruit-production was using a tomato harvesting robot and a robot to work in vineyard. Tomato harvesting robot consisted of manipulator, end-effector, visual sensor and traveling device. Plant training system of larger size tomato is similar with that of cherry-tomato. Two end-effectors were prepared for larger size tomato and cherry-tomato fruit harvesting operations, while the res components were not changed for the different work objects. A color TV camera could be used for the both work objects, however fruit detecting algorithm and extracted features from image should be changed. As for the grape-robot , several end-effector for harvesting , berry thinning , bagging and spraying were developed and experimented after attaching each end-effector to manipulator end. The manipulator was a polar coordinate type and had five degrees of freedom so that it could have enough working space for the operations. It was observed that visual sensor was necessary for harvesting, bagging and berry-thinning operations and that spraying operation requires another sensor for keeping certain distance between trellis and end-effector. From the experimental results, it was considered that multi-operations by the same robot could be appropriately done on the same or similar plant training system changing some robot components . One of the important results on having function of multi-operation was to be able to make working period of the robot longer.

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Effect of 1-Methylcyclopropene (1-MCP) Treatment on the Quality Characteristics and Pigmentation of Tomato Fruit (Lycopersicon Esculentum Mill.) (1-Methylcyclopropane 처리가 토마토 선도유지 효과 및 색소 변화에 미치는 영향)

  • Lee, Youn-Suk;Chung, Dae-Sung;Harte, Bruce R.;Shin, Joong-Min
    • Horticultural Science & Technology
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    • v.28 no.4
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    • pp.600-608
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    • 2010
  • The quality attributes of tomato fruit ($Lycopersicon$ $Esculentum$ Mill.) to treatments with 1-methylcyclopropene (1-MCP) were studied. Harvested tomato fruit was treated one time at the initiation of storage or once-a-day during storage with $1{\mu}L/L$ 1-MCP at different storage temperatures, 12, 17, and $23^{\circ}C$. The results showed that both lower temperature and duration of 1-MCP treatment played an important role in ripening of tomato fruit. The once-a-day 1-MCP treatment was presented to be very effective in delaying quality changes of tomato fruit. The amount of chlorophyll and lycopene were measured to assess the impact of 1-MCP and temperature treatments on ripening, using a specific extinction coefficient absorbance technique. Storing tomato fruit at $12^{\circ}C$ resulted in a longer ripening period (color change) than tomato fruit stored at $17^{\circ}C$ and $23^{\circ}C$. 1-MCP treatment was very effective in retarding chlorophyll degradation and lycopene formation in the pericarp tissue of the tomato fruit at the different storage temperatures. The 1-MCP treatments affected the total chlorophyll content in different fruit tissues of the pericarp and placenta. Exposure of tomato fruit to 1-MCP gas at $12^{\circ}C$, using the once-a-day treatment, was highly effective in delaying pigment and color change.

Evaluation of Parthenocarpic Fruit Set in Lycopersicon esculentum Mill (토마토의 단위결과성 재료 탐색)

  • Kim, Ji-Kwang;Kim, Jin-Han;Yoon, Wha-Mo;Park, Kwon-Seo;Park, In-Hee;Im, Um-Ryang
    • The Journal of Natural Sciences
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    • v.11 no.1
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    • pp.99-103
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    • 1999
  • This study was carried out to use as the basic data for breeding by evaluating parthenocarpy with 12 tomato lines and 17 allied species. Fruit set of open pollination plots was over 90% in 11 lines(CLN430-85-13-5 etc), 9.1-50% in 10 lines(LA1306 etc.), and the rest no fruit set. Fruit set after emasculation resulted highly 51.4% in CLN431-85-13-12, 53.9% in CLN425-130-8-1, 66.7% in CLN435-185-4-9, and 72.2% in Ventura, respectively. But fruit set in other tomato lines resulted under 50% and 17 allied species(LA1306 etc) resulted no effect of fruit set. Fruits other emasculation had no seeds and fruits after open pollination had 10-70 seeds per fruit. This result of this work showed that 3 lines, CLN435-185-4-9, CLN425-130-8-1, and CLN431-85-13-12, resulted in good effectiveness on the evaluation of parthenocarpy in tomatoes.

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Effect of Bacillus mesonae H20-5 on Fruit Yields and Quality in Protected Cultivation

  • Yoo, Sung-Je;Kim, Jeong Woong;Kim, Sang Tae;Weon, Hang-Yeon;Song, Jaekyeong;Sang, Mee Kyung
    • Research in Plant Disease
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    • v.25 no.2
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    • pp.84-88
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    • 2019
  • A variety of microorganisms in rhizosphere affect plant health by plant growth promotion, mitigation of abiotic stresses as well as protection from pathogen attacks. In our previous study, we selected a bacterium, Bacillus mesonae H20-5, for alleviation of salinity stress in tomato plants. In this study, we verified the effect of a liquid formulation of B. mesonae H20-5 (TP-H20-5) on fruit production and phytochemical accumulation including lycopene and polyphenol in cherry tomato and strawberry fruits in on-farm tests of protected cultivation under salinity stress. When vegetables including tomato, cherry tomato, strawberry, and cucumber were treated with TP-H20-5 by irrigated systems, final marketable yields were increased by 21.4% (cherry tomato), 9.3% (ripen tomato), 120.6% (strawberry), and 14.5% (cucumber) compared to untreated control. Moreover, treatment of TP-H20-5 was showed increase of phytochemicals such as lycopene and total polyphenol compared to untreated control in cherry tomato and strawberry. Therefore, these results indicated that a formulant of B. mesonae H20-5 can be used as a potential biofertilizer for increasing fruit production and quality.

Single Nucleotide Polymorphisms linked to the SlMYB12 Gene that Controls Fruit Peel Color in Domesticated Tomatoes (Solanum lycopersicum L.)

  • Kim, Bichsaem;Kim, Nahui;Kang, Jumsoon;Choi, Youngwhan;Sim, Sung-Chur;Min, Sung Ran;Park, Younghoon
    • Horticultural Science & Technology
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    • v.33 no.4
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    • pp.566-574
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    • 2015
  • Yellow or transparent fruit peel color is caused by the accumulation or lack of naringenin chalcone (NG, C) in fruit peel and determines the red or pink appearance of tomato fruit, respectively. NGC biosynthesis is regulated by the SlMYB12 gene of the Y locus on chromosome 1, and DNA markers derived from SlMYB12 would be useful for marker-assisted selection (MAS) of tomato fruit color. To develop a gene-based marker, 4.9 kb of the SlMYB12 gene including a potential promoter region was sequenced from the red-fruited (YY) line 'FCR' and pink-fruited (yy) line 'FCP'. Sequence alignment of these SlMYB12 alleles revealed no sequence variations between 'FCR' and 'FCP'. To identify SlMYB12-linked single nucleotide polymorphisms (SNPs), 'FCR' and 'FCP' were genotyped using a SolCAP Tomato SNP array and CAPS markers (CAPS-456, 531, 13762, and 38123) were developed from the four SNPs (solcap_snp_sl_456, 531, 13762, and 38123) most closely flanking the SlMYB12. These CAPS markers were mapped using $F_2$ plants derived from 'FCR' ${\times}$ 'FCP'. The map positions of the fruit peel color locus (Y) were CAPS-13762 (0 cM) - 456 (11.09 cM) - Y (15.71 cM) - 38123 (17.82 cM) - 531 (30.86 cM), and the DNA sequence of SlMYB12 was physically anchored in the middle of CAPS-456 and CAPS-38123, indicating that fruit peel color in domesticated tomato is controlled by SlMYB12. A total of 64 SolCAP tomato germplasms were evaluated for their fruit peel color and SNPs located between solcap_snp_sl_456 and 38123. Seven SNPs that were detected in this interval were highly conserved for pink-fruited accessions and specific to transparent fruit peel traits, as depicted by a phenetic tree of 64 accessions based on the seven SNPs.

Effect of Elevated Carbon Dioxide Concentration and Temperature on Yield and Fruit Characteristics of Tomato (Lycopersicon esculentum Mill.) (이산화탄소 및 온도 상승이 토마토 수량 및 과실특성에 미치는 영향)

  • Lee, In-Bog;Kang, Seok-Beom;Park, Jin-Myeon
    • Korean Journal of Environmental Agriculture
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    • v.27 no.4
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    • pp.428-434
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    • 2008
  • The objective of this study is to investigate the effect of the level of $CO_2$ (370 and $650{\mu}mol\;mol^{-1}$) and temperature (ambient and ambient+$5^{\circ}C$) on tomato growth and fruit characteristics as affected by the application rate of N-fertilizer (68 and $204\;N\;kg\;ha^{-1}$), for the purpose of evaluating the influence of elevated $CO_2$ and temperature on tomato crop. The elevated atmospheric $CO_2$ and temperature increased the plant height and stem diameter for tomato crop, while the differences among the nitrogen(N) application rates were not significantly different. Under the elevated $CO_2$, temperature, and a higher N application rate, the biomass of aerial part increased. The fruit yield showed the same result as the biomass except for the elevated temperature. The elevated temperature made the size of fruit move toward the small, but the elevated $CO_2$ and the application of N-fertilizer were vice versa. The sugar content and pH of fruit juice were affected by nitrogen application rate, but not by the elevated $CO_2$ and temperature. These results showed that both the elevated $CO_2$ and temperature stimulated the vegetative growth of aerial parts for tomato, but each effects on the yield of fruit showed an opposite result between the elevated temperature and $CO_2$. In conclusion, the elevated $CO_2$ increased tomato yield and the ratio of large size of fruit, but the elevated temperature did not. Therefore, to secure the productivity of tomato as nowadays in future environment, it will need to develop new breeder as high temperature-tolerable tomato species or new type of cropping systems.

A study to Establish on Over-all system for Nationalized way of Agriculture (자연농법을 위한 종합적 체계수립에 관한 연구(I)BLCS(생균제) 미생물비료의 TOMATO 시비에 관한 연구)

  • 김경제
    • Korean Journal of Organic Agriculture
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    • v.6 no.1
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    • pp.77-84
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    • 1997
  • The results of application of the organic manure obtained from livestocks fed with BLCS in the Tomato under structure were as follow. The No. of Fruit weight, Fruit width, Fruit length and Sugar content of Tomato in the Dung of fowl(BLCS) were high in the other treatment, second was cattle dropping(BLCS), third was Pig dropping(BLCS). The contents of Total N, P2O5, K2O, CaO, and MgO in the Tomato fruit of the cattle dropping(BLCS), the Pig dropping(BLCS), and the Dung of fowl(BLCS) were high than animal dropping plots.

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