• Title, Summary, Keyword: tannins

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Inhibitory Effects of Tannins on Tyrosinase Activity (탄닌화합물의 Tyrosinase 억제 활성)

  • Cho, Su-Min;Kim, Jee-Hun;Lee, Min-Won
    • Korean Journal of Pharmacognosy
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    • v.32 no.1
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    • pp.68-71
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    • 2001
  • For the use of tannins in the whitening-effect cosmetics, inhibitory effect against tyrosinase activity was determined. Three condensed tannins including gallocatechin, gallocatechin 3',4'-di-O-gallate and epicatechin 3-O-gallate and three hydrolyzable tannins, 1,2,6-tri-O-galloyl-${\beta}$-D-glucose, 2,3-(S)-HHDP-D-glucose and pedunculagin showed 15-29% mild inhibitory effects against tyrosinase activity.

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Physiological Characteristics of Tannins isolated from Astringent Persimmon Fruits (떫은감에서 분리한 탄닌성분의 기능적 특성)

  • Seo, Ji-Hyung;Jeong, Yong-Jin;Kim, Kwang-Soo
    • Korean Journal of Food Science and Technology
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    • v.32 no.1
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    • pp.212-217
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    • 2000
  • This study was determined protein reaction, antioxidative activity, nitrite scavening ability and antimicrobial activity of tannins isolated from astringent persimmon fruits. Tannins extracted from green persimmon fruits reacted highly with BSA(bovine serum albumin). Reactions between tannins and BSA were more active when contents of tannin were higher than that of BSA. Antioxidative abilities of green persimmon tannin were comparable to that of BHT(butylated hydroxytoluene). Green persimmon tannins exhibited remarkable nitrite-scavenging activity. Different antimicrobial activities of persimmon tannins were observed depending on the maturity. The growth of V. parahaemolyticus and E coil were highly inhibited by the addition of persimmon tannins. Tannins from soft persimmon did not have antimicrobial activities against B. subtilis and S. typhimurium.

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Changes in Pattern of Tannin Isolated from Astringent Persimmon Fruits (떫은감에서 분리한 탄닌성분의 패턴 변화)

  • 서지형;정용진;신승렬;김주남;김광수
    • Korean Journal of Food Preservation
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    • v.6 no.3
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    • pp.328-332
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    • 1999
  • This study was investigated pattern changes of tannin isolated from astringent persimmon fruits. The contents of total phenolics and soluble tannins decreased as the maturing and softening of persimmon fruits proceeded. Green and mature persimmon tannins reacted with acetaldehyde. The more contents of tannin increased, the more reactions became. And the reaction of green Persimmon tannin was more active than mature persimmon tannins. But tannin from soft persimmon fruits did not react with acetaldehyde. Tannins were more polymerized during maturing and softening of fruits. So there was a little difference in chromatography of persimmon tannins. Also green and mature persimmon tannins obtained 4 bands respectively after thin layer chromatography analysis. But there was only 2 bands in soft persimmon tannin. As softening of persimmon fruits proceeded, most of tannins reacted with acetaldehyde, so coagulated. Also the component of soluble tannins was changed during softening of persimmon fruits.

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Inhibitory Effect of Condensed Tannins Isolated from Korean Green Tea against Xanthine Oxidase (한국산 녹차로부터 분리한 축합형 탄닌의 Xanthine Oxidase 저해효과)

  • Cho, Young-Je;Chun, Sung-Sook;Choi, Cheong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.4
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    • pp.418-422
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    • 1993
  • For the purpose of utilizing tannins in the functional foods and crude drugs the xanthine oxidase inhibition of tannins isolated from Korean green tea was determined. Acetone extract from Korean green tea showed inhibitory effect against the xanthine oxidase. The galloyl tannins showed higher inhibitory activity against xanthine oxidase than the nongalloyl tannins. In terms of stereo isomers, (-)-epicatechins had higher inhibitory activity than the (+)-catechins. The synergistic activity was also observed. Tannins isolated from Korean green tea appeared to be incompetitive inhibitor against the xanthine oxidase.

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Determination of Flavonoids, Tannins and Ellagic Acid in Leaves from Rubus L. Species

  • Gudej, Jan;Tomczyk, Michal
    • Archives of Pharmacal Research
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    • v.27 no.11
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    • pp.1114-1119
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    • 2004
  • This paper describes the quantitative determination of flavonoids, tannins and ellagic acid in the leaves from wild and cultivated variations of Rubus L. species (Rosaceae): raspberry (2 wild and 13 cultivars) and blackberry (3 wild and 3 cultivars). The content of flavonoids was analyzed using spectrophotometric (the Christ-M llers method) and HPLC analysis after acid hydrolysis. The content of tannins was determined by the weight method, with hide powder, described by German Pharmacopoeia 10 (DAB 10). Ellagic acid content was examined using the HPLC method after acid hydrolysis. Flavonoid content, determined using the Christ-Muller's method was higher for the blackberry leaves than for the raspberry leaves and varied between 0.46% and 1.05%. Quercetin and kaempferol were predominant in all samples analyzed using the HPLC method. The highest flavonoid content was found in the leaves of R. nessensis (1.06%); with results in all of the examined samples varying between 0.27% and 1.06%. The concentration of ellagic acid in all species was determined after acid hydrolysis and ranged from 2.06% to 6.89%. The leaves of raspberries are characterized by greater amounts of tannins (varying between 2.62% and 6.87%) than the leaves of other species. The results from this study indicate that the analyzed species are a rich source of flavonoids, ellagic acid and tannins, which may be used for the quality assessment of Rubus L. species leaves.

Inhibition Effect of Against Angiotensin Converting Enzyme of Flavan-3-ols isolated Korean Green Tea (한국산 녹차로부터 분리한 Flavan-3-ol 화합물의 Angiotensin Converting Enzyme 저해 효과)

  • Cho, Young-Je;An, Bong-Jeun;Choi, Cheong
    • Korean Journal of Food Science and Technology
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    • v.25 no.3
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    • pp.238-242
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    • 1993
  • For the Purpose of utilizing tannins in the functional foods and crude drugs, the enzyme inhibition of tannins isolated from Korean green tea were determined. Acetone extract from Korean green tea showed inhibition effect against the angiotensin converting enzyme. The galloyl tannins showed higher inhibition activity against angioteosin converting enzyme than the nongalloyl tannins. In terms of stereo isomers, (-)-epicatechins had higher inhibition activity than the (+ )-catechins. The synergistic activity was also observed. Tannins isolated from Korean green tea appeared to be incompetitive inhibitor against the angiotensin converting enzyme.

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Effect of Wastewater Treatment with Tannins from Peel of Astringent Persimmon Fruits (떫은감 껍질로부터 분리한 탄닌을 이용한 폐수처리 효과)

  • Cho Young-Je;Chun Sung-Sook
    • Korean Journal of Food Preservation
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    • v.12 no.3
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    • pp.299-304
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    • 2005
  • The two major tannins were separated by Sephadex LH-20 and MCI-gel CHP-20 from peel of astringent persimmon fruits. Purified tannins were identified to (+)-catechin and (+)-gallocatechin by NMR, IR spectrum and FAB-mass spectrum. The removal rate of turbidity, T-N, T-P and CODcr in wastewater with lime and (+)-gallocatechin was higher than those of (+)-catechin because (+)-gallocatechin has more hydroxyl groups. As increasing concentration of tannins from peel of astringent persimmon fruits, the removal rate of turbidity, T-N, T-P and CODcr were increased. Synergistic activity by mixed tannins(catechin+gallocatechin) was also observed.

Variation of the Contents of Triterpenoids and Tannins Depending on Growth and Infection in the Leaves of Rubus crataegifolius and Rubus parvifolius

  • Kim, Min-Young;Rhim, Tae-Jin;Lim, Sang-Cheol;Park, Hee-Juhn
    • Natural Product Sciences
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    • v.21 no.1
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    • pp.14-19
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    • 2015
  • Several $19{\alpha}$-hydroxyursane-type triterpenoids and hydrolysable tannins have beneficial effects on human health. Rubus crataegifolius (Rosaceae) has the cleft simple leaf whereas R. parvifolius has pinnate compound leaves. This research was aimed to find the variation in the contents of the triterpenoids and tannins between the infected versus uninfected leaves of R. coreanus and R. parvifolius and between young versus mature leaves. Triterpenoids and tannins were quantitatively analyzed by HPLC. Six triterpenoids including tormentic acid, euscaphic acid, 23-hydroxytormentic acid, coreanoside $F_1$, kaji-ichigoside $F_1$ and niga-ichigoside $F_1$ were used for standard compounds. Gallotannins and ellagitannins were quantitatively evaluated using the indicatives of methyl gallate and ellagic acid. The infected leaves of R. crataegifolius contained higher levels of triterpenoids and tannin than the uninfected leaves; however, lower quantity of total tannin was observed in the mature leaves than in the young leaves. Although the pinnate compound leaves of R. parvifolius exhibited similar tendency of those compositional variation with R. crataegifolius each other, its contents of triterpenoids do not considerably vary. Variation of the contents of triterpenoids and tannins were particularly distinct in R. crataegifolius by growth and infection.

Antioxidative Activity of Tannins from Rubus coreanum (복분자딸기에서 분리한 탄닌화합물의 항산화작용)

  • 김광호;이연아;김준식;이도익;최영욱;김하형;이민원
    • YAKHAK HOEJI
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    • v.44 no.4
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    • pp.354-357
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    • 2000
  • Two ellagitannins, pedunculagin and 2,3-(S)-Hexahydroxydiphenoyl (HHDP)-D -glucose and three condensed tannins, (+)-catechin, (-)-epicatechin and procyanidin B-4 which were isolated from Rubus coreanum were evaluated for their antioxidative effects with 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and lipid peroxidation generation system mediated by addition of $H_2O$$_2$to rat liver homogenate (TBARS). The ellagitannins, 2,3-(S)-HHDP-D-glucose and pedunculagin, showed more potent antioxidative activities by DPPH and TBARS than condensed tannins.

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Tannins from Rubus coreanum

  • Lee, Yeon-Ah;Lee, Min-Won
    • Korean Journal of Pharmacognosy
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    • v.26 no.1
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    • pp.27-30
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    • 1995
  • Tannins were isolated from the stems of Rubus coreanum and identified as (-)-epicatechin, (+)-catechin, procyanidin B-4 and sanguiin H-4 by spectral analysis.

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