• Title, Summary, Keyword: steroidal saponins

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Mutagenic Effect of Steroidal Saponins from Smilax china Rhizomes (토복령(Smilax china)의 Steroid Saponin이 돌연변이원성에 미치는 영향)

  • Kim, Sung-Whan;Son, Kun-Ho;Chung, Kyu-Charn
    • YAKHAK HOEJI
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    • v.33 no.5
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    • pp.285-289
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    • 1989
  • Pontential mutagenic and antimutagenic activities of four steroidal saponins from Smilax china rhizomes were investigated. These saponins did not revealed mutagneicity in the Ames and SOS umu test. For antimutagenic activity by SOS umu test, two spirostanol glycosides, dioscin and gracillin, inhibited the activity of ${\beta}-galactosidase$ induced by AF-2, but their proto-type furostanol glycosides did not show this activity.

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Inhibition of Mouse Ear Edema by Steroidal and Triterpenoid Saponins

  • Kim, Sung-Yong;Son, Kun-Ho;Chang, Hyeun-Wook;Kang, Sam-Sik;Kim, Hyun-Pyo
    • Archives of Pharmacal Research
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    • v.22 no.3
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    • pp.313-316
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    • 1999
  • Certain steroids and triterpenoids isolated from diverse plant families were known to posses anti-inflammatory activity. In the course of finding new anti-inflammatory natural products, some steroidal and triterpenoid saponins were isolated and evaluated for their anti-inflammatory activity using in vivo mouse ear edema test. At the oral dose of 100 mg/kg, several steroidal saponins and triterpenoid saponins such as hederagenin glycosides showed significant inhibition of ear edema (20∼37% inhibition), though less potent than indomethacin and hydrocortisone.

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Identification And Quantification of Steroidal Saponins in Polygonatum Species by HPLC/ESI/MS

  • Ahn, Mi-Jeong;Kim, Jin-Woong
    • Archives of Pharmacal Research
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    • v.28 no.5
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    • pp.592-597
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    • 2005
  • An HPLC/Esl/MS method has been developed to identify and quantify the spirostanol glycosides in the rhizomes of five Polygonatum species. The relative distribution of two steroidal saponins in each extract was established using the selective ion monitoring (SIM) mode via an electrospray ionization (ESI) source. It was found that there were significant differences in the amount of spirostanol glycosides among the Polygonatum Species. The results showed that this method could be used to identify the steroidal saponins in the extracts and differentiate Polygonatum species with high sensitivity and reproducibility in a short time. Fragmentation patterns of the two reference compounds were also discussed with the electrospray ionization multi-stage tandem mass spectroscopy (ESI-MS$^n$).

Steroidal Saponins from Paris polyphylla Induce Apoptotic Cell Death and Autophagy in A549 Human Lung Cancer Cells

  • He, Hao;Sun, Yan-Ping;Zheng, Lei;Yue, Zheng-Gang
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.3
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    • pp.1169-1173
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    • 2015
  • Background: Paris polyphylla (Chinese name: Chonglou) had been traditionally used for a long time and shown anti-cancer action. Based on the previous study that paris polyphylla steroidal saponins (PPSS) induced cytotoxic effect in human lung cancer A549 cells, this study was designed to further illustrate the mechanisms underlying. Materials and Methods: The mechanisms involved in PPSS-induced A549 cell death were investigated by phase contrast microscopy and fluorescence microscopy, flow cytometry and western blot analysis, respectively. Results: PPSS decreased the proportion of viable A549 cells, and exposure of A549 cells to PPSS led to both apoptosis and autophagy. Apoptosis was due to activations of caspase-8, caspase-3, as well as cleavage of PARP, and autophagy was confirmed by up-regulation of Beclin 1 and the conversion from LC3 I to LC3 II. Conclusions: PPSS was able to induce lung cancer A549 cell apoptosis and autophagy in vitro, the results underlining the possibility that PPSS would be a potential candidate for intervention against lung cancer.

Steroidal Saponins from Paris polyphylla Suppress Adhesion, Migration and Invasion of Human Lung Cancer A549 Cells Via Down-Regulating MMP-2 and MMP-9

  • He, Hao;Zheng, Lei;Sun, Yan-Ping;Zhang, Guang-Wei;Yue, Zheng-Gang
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.24
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    • pp.10911-10916
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    • 2015
  • Background: Tumor metastases are the main reasons for oncotherapy failure. Paris polyphylla (Chinese name: Chonglou) has traditionally been used for its anti-cancer actions. In this article, we focus on the regulation of human lung cancer A549 cell metastases and invasion by Paris polyphylla steroidal saponins (PPSS). Materials and Methods: Cell viability was evaluated in A549 cells by MTT assay. Effects of PPSS on invasion and migration were investigated by wound-healing and matrigel invasion chamber assays. Adhesion to type IV collagen and laminin was evaluated by MTT assay. Expression and protease activity of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, were analyzed by Western blotting and gelatin zymography, respectively. Results: PPSS exerted growth inhibitory effects on A549 cells, and effectively inhibited A549 cell adhesion, migration and invasion in a concentration-dependent manner. Western blotting and gelatin zymography analysis revealed that PPSS inhibited the expression and secretion of MMP-2 and MMP-9 in A549 cells. Conclusions: PPSS has the potential to suppress the migration, adhesion and invasion of A549 cells. PPSS could be a potential candidate for interventions against lung cancer metastases.

Two New Steroidal Saponins from the Tuberss of Liriope spicata

  • Lee, Do-Yong;Son, Son-Ho;Do, Jae-Chul;Kang, Sam-Sik
    • Archives of Pharmacal Research
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    • v.12 no.4
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    • pp.295-299
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    • 1989
  • Two novel steroidal saponins designated as spicatosides A(1) and B(2) were isolated from the tubers of Liriope spicata and their structures were elucidated as 25(S)-rus-cogenin -1-0- fJ - D-glucopyranosyl (1$\rightarrow$2)- [$\beta$ - D-xylopyranosyl (1$\rightarrow$3)] -$\beta$- D- fucopyranoside (1) and 26-0-$\beta$-D-glucopyranosyI25(S)-22-0-methyl-furost-5-en-l$\beta$, 3$\beta$, 26-trioll-0-fJ -D¬glucopyranosyl (1$\rightarrow$2)- [$\beta$- D-xylopyranosyl (1$\rightarrow$3)] - $\beta$- D- fucopyranoside (2), respectively.

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In vitro Culture of Adventitious Roots from Dioscorea nipponica Makino for the Production of Steroidal Saponins (부채마 (Dioscorea nipponica Makino)의 부정근 배양조건과 Steroidal Saponin의 기내생산)

  • An Jung-Hee;Son Kun-Ho;Sohn Ho-Yong;Kwon Soon-Tae
    • Journal of Plant Biotechnology
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    • v.32 no.3
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    • pp.217-223
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    • 2005
  • Effects of growth regulators on growth of adventitious roots and accumulation of steroidal saponins, such as dioscin, prosapogenin A and prosapogenin C, in cultures of Dioscorea nipponica were determined. The maximum growth of adventitious roots was observed in MS medium supplemented with 30 g/L sucrose and 1.0 mg/L NAA. Addition of BA in combination with NAA appeared to be no effective in the growth of adventitious roots. Among the twenty different adventitious roots formed from different seeds, strain No. 10 was selected based on production ability of dioscin, and its stability through the successive liquid culture. During the first 4 weeks of incubation, contents of steroidal saponins in adventitious roots were negligible but the contents were markedly increased at 5 weeks of incubation. Dioscin and prosapogenin C content in IBA-treated adventitious roots were significantly higher than those in NAA-treated roots. However, content of prosapogenin A was not significantly different among NAA or IBA level. Results provide that liquid culture of adventitious roots of D. nipponica have a potential for mass production of dioscin including prosapogenin A and prosapogenin C.

Steroidal Saponins from the Rhizomes of Smilax china

  • Kim, Sung-Whan;Chung, Kyu-Charn;Son, Kun-Ho;Kang, Sam-Sik
    • Korean Journal of Pharmacognosy
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    • v.20 no.2
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    • pp.76-82
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    • 1989
  • Five known diosgenin glycosides have been isolated from the MeOH extract of Smilax china rhizomes and characterized as prosapogenin A of dioscin, dioscin, gracillin, methyl protogracillin, methyl protodioscin and its corresponding 22-hydroxy analog. This is the first isolation of the former four compounds from this plant. ${\beta}-Sitosterol\;glucoside$ was also isolated and identified.

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Further Spirostanol Glycosides from the Tuber of Liriope spicata

  • Do, Jae-Chul;Sung, Yong-Kyung;Son, Kun-Ho
    • Korean Journal of Pharmacognosy
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    • v.22 no.2
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    • pp.73-77
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    • 1991
  • Further studies have been done on the constituents of the tubers of Liriope spicata $L_{OUR}$ (Liliaceae). Four steroidal glycosides, tentatively designated as compounds I. II, III and IV, were isolated from the n-BuOH soluble fraction of this plant. The structures of these glycosides were established as ${\beta}-sitosterol$ glucoside, prosapogenin II of spicatoside A, ophiopogonin B, and prosapogenin III of spicatoside A.

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