• Title/Summary/Keyword: soy protein

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Effects of Specific Interaction Altering Reagents on Hardnesses of Succinylated Soy Protein Gel

  • Bae, Dongho;Jung, Hosun;Choi, Yong-Hee
    • Journal of Applied Biological Chemistry
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    • v.42 no.3
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    • pp.125-129
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    • 1999
  • The changes in gel characteristics of soy protein and succinylated soy protein due to various specific interaction-altering reagents which affect the formation and textural properties of gels, were studied. The reagents were added to 15% soy protein solutions prior to heat treatment. Succinylated soy protein formed harder gel without the addition of reagents. Hardly no gels were formed with urea, indicating that hydrogen bonds significantly contributed to the formation and hardness of the gel and the effects of urea on the hardness of succinylated soy protein gel were more significant. Disulfide bonds were important in the formation of hard gels whether they were succinylated or not, but the contributions of hydrophobic interactions to gel hardness were relatively insignificant. The hardness reducing effects of NaCl and NaSCN were more significant in succinylated soy protein gel. As such, electrostatic interactions were important for succinylated soy protein to form hard gel but not for unmodified soy protein.

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Effects of Soy Protein and Isoflavones on Bone Mineral Density in Crowing Female Rats (콩단백질과 이소플라본이 성장기 암컷 쥐의 골밀도에 미치는 영향)

  • 최미자;조현주
    • Journal of Nutrition and Health
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    • v.36 no.4
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    • pp.359-367
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    • 2003
  • The purpose of this study was to examine the impact of soy protein and soy isoflavones on bone and mineral density in young female Sprague-Dawley rats. Fifty eight rats (body Weight 75 $\pm$ 5 g) were randomly assigned to one of four groups, consuming casein, soy protein concentrate, soy protein isolate (57 mg isoflavones/100 g diet) or casein added isoflavones (57 mg isoflavones /100 g diet). All rats were fed on experimental diet and deionized water ad libitum for 9 weeks. Bone mineral density (BMD) and bone mineral content (BMC) were measured using PIXImus (GE Lunar Co, Wisconsin, USA) in spine and femur on 3, 6, 9 weeks after feeding. The serum and urine concentrations of Ca and P were determined. Diet did not affect weight gain and mean food intake. Food efficiency ratio was lower In soy protein groups. The serum concentration of Ca and P were not changed by soy protein and isoflavones. Urinary Ca and P excretion were not significantly different. Spine BMD was significantly increased by soy protein isolate on 3 and 6 weeks after feeding. Femur BMD was significantly increased in the groups of soy protein isolate and isoflavones adding on after 9 weeks. Therefore, soy protein with rich isoflavones may be beneficial on spine and femur BMD increasement in growing female rats.

Effects of Phosphorylation and Acetylation on Functional Properties and Structure of Soy Protein (인산화와 초산화가 대두단백질의 기능특성과 구조에 미치는 영향)

  • Kim, Nam-Soo;Kwon, Dae-Young;Nam, Young-Jung
    • Korean Journal of Food Science and Technology
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    • v.20 no.5
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    • pp.625-630
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    • 1988
  • Phosphorylation of soy protein by sodium trimetaphosphate and acetylation of soy protein by acetic anhydride were performed. Then, the functional properties of modified soy proteins were compared with that of unmodified soy protein. Isolated soy protein prepared from defatted soybean flake had protein content of 92.7% as moisture-free basis. The phosphorylated soy protein showed higher solubility, foaming properties, and water holding capacity than unmodified soy protein. Acetylation of soy protein increased emulsification activity and foaming properties greatly, whereas decreased the solubility at pH 8.0. Isoelectric pHs of phosphorylated and acetylated soy protein were shifted to acidic regions(pH 3.0 and pH 4.0) from pH 5.0, which was the isoelectric pH of unmodified soy protein. Soy protein seems to be aggregated during phosphorylation and acetylation procedure, judging form Sepharose CL-4B gel filtration profiles. The modified soy proteins showed increased mobilities to anode direction in disc-gel electrophoresis.

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The Effect of Meat Protein and Soy Protein on Calcium Metabolism in Young Adult Korean Women (단백질의 종류가 체내 칼슘 대사에 미치는 영향에 관한 연구)

  • 피재은
    • Journal of Nutrition and Health
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    • v.19 no.1
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    • pp.32-40
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    • 1986
  • The present study conducted to examine the effects of proteins from different sources on Ca excretion in 6 healthy young adult Korean women. The subjects were given meat protein diet for 5 days and soy protein diet for subsequent 5 days. the two diets were similar in protein and Ca contents. Urinary and fecal samples were collected for the last 2 days of each diet period and were analyzed for Ca and P. The results were as following ; 1) Mean daily urinary calcium excretion was 126.5$\pm$22.70mg for meat protein diet and 83.7$\pm$17.19mg for soy protein diet and the difference was significant (P<0.025). 2) Fecal Ca excretion did not show significant difference between two experimental preiod ; 466.9$\pm$73.68 mg of meat portein diet 284.4$\pm$54.96mg for soy protein diet. 3) Three out of six subjects showed negative balance on meat protein diet, but only one showed negative balance on soy protein diet. The averageof the balances on meat protein diet was -65.4 $\pm$68.19 and that of soy protein diet was 155.3$\pm$52.28 ; the difference was significant(P<0.025). 4) Urinary P excretion tended to be higher on meat protein diet but was not significant compared to soy protein diet . Fecal P excretion was significantly higher (P<0.001) on soy protein diet. Overall P balances for meat protein diet and soy protein diet were 219.94 mg and 229.46mg respectively (P<0.05). The above results show that urinary Ca excretion was significantly higher on meat protein diet but fecal excretion did not show significant difference between meat protein diet and soy protein diet. The overall Ca balance was significantly higher on Soy protein diet compared to meat protein diet.

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Studies on Busuge Preparation -II. Effect of the Addition of Soy Products on the Quality of BISIGE(SAN-JA) Base- (부수게 제조에 관한 연구 -제 2보 대두 첨가가 부수게(산자) 바탕의 품질에 미치는 영향-)

  • Kim, Joong-Man;Wei, Lun-Shin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.14 no.1
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    • pp.51-56
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    • 1985
  • This study was to compare the effects of soy hot water extract, soy slurry, defatted soy flour, soy protein concentration and soy protein isolate on the quality of Busuge Base. In Busuge Base preparation the addition of soy products, in general, showed the improvable effect in the quality of Busuge Base. Among them the soy slurry was especially effective in volume increasing, hardness and panel score, and the soy protein isolate was effective in the protein fortification of Busuge Base. In addition, the qualify of Busuge Base was best when soy slurry and soy protein isolate were jointly used. In this case, the adequate amount of soy protein isolate was about 10%.

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Effects of Garlic, Soy Protein and Taurine on Blood Lipid Profiles in Postmenopausal Hyperlipidemic Model Rats

  • Cheong, Sun-Hee;Chang, Kyung-Ja
    • Proceedings of the KSCN Conference
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    • 2004.05a
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    • pp.437.2-438
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    • 2004
  • The purpose of this study was to investigate the effects of garlic, soy protein and taurine on blood lipid profiles in postmenopausal hyperlipidemic model rats. Seventy-two female Sprague-Dawley rats weighing 200g were fed a commercial diet for one week. All rats were ovariectomized and then were randomly assigned to eight groups (high fat diet group; HFD group, 2% garlic powder group; GP group, taurine group; TR group, soy protein group; SP group, 2% garlic powder + taurine group; GP+TR group, 2% garlic powder+ soy protein group; GP+SP group, taurine+soy protein group; TR+SP group, 2% garlic powder+taurine+soy protein group; GP+TR+SP group).(omitted)

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Effects of Dietary L-Carnitine Supplementation on Lipid Metabolism in Rats fed with Different Types Protein (단백질의 종류가 다른 식이에 L-카르니틴을 첨가했을 때 흰쥐의 지질대사에 미치는 영향)

  • 원향례
    • The Korean Journal of Community Living Science
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    • v.15 no.3
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    • pp.113-120
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    • 2004
  • This study is to investigate the effect of dietary L-carnitine supplementation on lipid metabolism in rats fed with isolated soy protein and casein for their source of protein. Four experimental groups were organized and each group had eight Sprague-Dawley male rats with the initial weight of around 180g. The four groups were CO (casein only supplemented group); CC (casein and 3% L-carnitine supplemented group); ISO (isolated soy protein only supplemented group); ISC (isolated soy protein and 3% L-carnitine supplemented group). All groups were supplemented with the experimental diet for four weeks and carnitine comprised 3% of. their diet. The results were as follows; 1. There was no significant difference in food intake among the groups. 2. Final weight gain was significantly lower in the groups supplemented with isolated soy protein than in the groups supplemented with casein (P<0.05). The groups with supplemented casein and carnitine showed the effect of weight reduction (p<0.05). 3. Food efficiency ratio was lower in the groups supplemented with isolated soy protein than in the groups supplemented with casein (p<0.01). The groups supplemented with casein and carnitine showed low food efficiency ratio. 4. The serum total lipid was higher in the groups supplemented with casein than in the groups supplemented with isolated soy protein (p<0.05). 5. Serum total cholesterol was higher in the groups supplemented with casein than in the groups supplemented with isolated soy protein. 6. There was no significant difference in triglyceride, HDL-cholesterol, and LDL-cholesterol in serum among the groups. 7. Out of the groups supplemented with isolated soy protein the total cholesterol level in liver was low in the groups to which carnitine was supplemented (p< 0.05). However, there was no significant difference of liver total lipid and triglyceride among the groups. 8. There was no difference in TBARS levels and GSH-Px activities in liver among the groups.

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Effects of Dietary Protein on Growth and Lipid Metabolism in Growing Rats (식이단백질조성이 흰쥐의 성장과 지방대사에 미치는 영향)

  • Kim, Yoo-Sook;Kim, Wha-Young
    • Journal of Nutrition and Health
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    • v.15 no.2
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    • pp.119-128
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    • 1982
  • The effect of dietary protein on growth and lipid levels of plasma and liver was studied in weanling male rats fed diets differing protein sources and amino acid balance. Rats were devided into 9 experimental diets which were grouped into 3 categories ; 1) Simple protein category includes gluten-, soy protein isolate-, and casein-containing diet groups, 2) Supplemented category includes casein supplemented with methionine, soy protein isolate supplemented with methionine, and gluten supplemented with lysine and methionine, 3) Mixed protein category includes diet groups containing gluten (2/3), casein (1/3), soy protein isolate (2/3) and casein (1/3), and casein (1/3), soy protein isolate (1/3) and gluten (1/3). The experimental diets composed of 15% protein, 65.8% carbohydrate, 10% fat and 1% cholesterol. The body wt. gain and P.E.R. were greater in rats of supplemented and mixed protein groups than simple protein groups. No statistical differences were found in plasma cholesterol among gluten, soy protein isolate and casein groups. Consumption of diets supplemented with limiting amino acid to gluten or soy protein isolate reduced the plasma cholesterol level by 23.2% and 34.2% respectively. However there was no difference between casein and the supplemented casein groups. The mixed protein groups shows relatively high plasma cholesterol concentration and low liver cholesterol levels. On the other hand gluten group showed low plasma cholesterol and high liver cholesterol levels, which means body cholesterol pool may not have been changed by the dietary protein. Feeding soy protein meal and the supplemented soy protein isolate resulted in lower plasma cholesterol, plasma triglycerides, liver cholesterol and liver triglycerides levels. This hypolipidemic effect is considered to see unique to soy protein isolate. Rats in gluten and the supplemented gluten groups showed lower plasma protein levels and a tendency of fatty liver.

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Effect of Genistein and Soy Protein on Lipids Metabolism in Ovariectomized Rats (난소절제 랫드에서 제니스테인과 콩단백질이 지질 대사에 미치는 영향)

  • Lee Young Min;Jung Myeong Ho;Lee Yeon Sook;Song Jihyun
    • Journal of Nutrition and Health
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    • v.38 no.4
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    • pp.267-278
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    • 2005
  • Postmenopausal women or ovariectomized rats are associated with increased cholesterol levels, which are risk factors of metabolic syndrome and cardiovascular diseases. Increased prevalence of metabolic syndrome after menopause might be associated with estradiol deficiency. Harmful effect of estradiol hampers the casual usage of hormone to prevent the metabolic syndrome. Soy protein has been reported to show several beneficial effects on health, however it is unclear which components of soy protein is responsible for anti-obesity and hypocholesterolemic effects. Soy isoflavones, gem-stein and daizein, are suggested to have anti-obesity and hypocholesterolemic effects but with inconsistency. The present study investigated the effect of supplementation of genistein (experiment I) and soy protein containing isoflavones (experiment II) to high fat diet on body weight gain, food intake, liver and fat tissue weight and the lipid levels in ovariectomized rats. Plasma and hepatic lipid contents and the mRNA levels of genes encoding lipid metabolism related proteins, such as CPT1 and HMGR were measured. Ovariectomy increased body weight, fat tissue weight and plasma and hepatic lipid levels which increase the risk of metabolic syndrome. Soy protein could improve plasma and hepatic lipids levels. Soy protein also increased hepatic CPT1 and HMGR mRNA levels. Plasma and hepatic lipids levels could not be decreased by dietary genistein alone. In contrast, lipids levels could be decreased by isoflavone-fortified soy protein, suggesting that the ingestion of soy protein enriched with isoflavone gives more benefit for protecting postmenopausal women from metabolic syndrome.

Effect of Soy Protein Hydrolyzate on Lipid Metabolism and Antioxidant Activity in the Rat (대두단백가수분해물이 흰쥐의 지질대사와 항산화에 미치는 영향)

  • Han, Yoon-Hee;Park, Sang-Kyu;Kim, Hye-Young
    • Journal of Nutrition and Health
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    • v.41 no.2
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    • pp.119-126
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    • 2008
  • This study was conducted to investigate the effect of soy protein hydrolyzate on lipid metabolism and antioxidant activity in the rat. Thirty-eight male rats of Sprague-Dawley strain were divided into five groups: casein, isolated soy protein (ISP), seoritae protein hydrolyzate (SH), soluble soy protein hydrolyzate (SS), and insoluble soy protein hydrolyzate (IS). The control diet (casein group) contained 20% casein protein and experimental diet contained 10% casein and 10% isolated soy-protein or soy-protein hydrolyzate. Fecal lipid content was increased and lipid apparent absorption rate was decreased significantly by the ISP group at the first week of experimental period. Blood triglyceride, total cholesterol, LDL-cholesterol and atherogenic index (AI) were decreased by soy protein hydrolyzate groups than casein group. Liver total lipid, triglyceride and cholesterol were not different among groups, but showed decreasing tendencies in soyprotein hydrolyzate groups. The lipid lowering effect was prominent in the IS group among soy protein hydrolyzate groups. Total antioxidant activity showed increasing tendency in the seoritae hydrolyzate group. Liver superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase activities also showed higher tendencies in the seoritae hydrolyzate group than other groups. In conclusion, insoluble soyprotein hydrolyzate was more effective in lowering body lipids and seoritae hydrolyzate had higher antioxidant capacity among soy protein hydrolyzates.