• Title, Summary, Keyword: soil actinomycetes

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Distribution of Oligotrophic Actinomycetes in Forest Soil. (산림 토양 중 저 영양성 방선균의 분포)

  • Jeong, Eun-Ye;Lee, Sang-Hwa;Park, Dong-Jin;Lee, Hyang-Burm;Piao, Zhe;Kim, Chang-Jin
    • Microbiology and Biotechnology Letters
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    • v.28 no.4
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    • pp.239-241
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    • 2000
  • From 5 forest soil samples, the isolation frequency of oligotrophic Actinomycetes was investigated with nutrient and minimal media. The frequency of soil Actinomycetes by the minimal media. The frequency of soil Actinomycetes by the minimal media of DHV, WA, BA and NA was similar to the value by HV nutrient medium. In addition, different Actinomycetes were isolated from all the media used in this experiment and then their growth were tested on nutrient (Bennett's agar) and minimal media(WA). In consequent, the number of different Actinomycetes from minimal media was 26.1 strains, whereas the number of from nutrient medium (HV) was 26.6 strains. Furthermore, the percentage of facultative and obligate strains among the oligotrophic Actinomycetes was 90% and 10%, respectively.

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Genus Diversity of Soil Actinomycetes Isolated from Natural Lime Cave. (자연 석회동굴에서 분리한 방선균의 속 다양성)

  • 박동진;이상화;박해룡;권오성;박상호;마사카즈우라모토;김창진
    • Microbiology and Biotechnology Letters
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    • v.28 no.3
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    • pp.129-133
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    • 2000
  • Different actinomycete strains were isolated from natural lime caves of Ondal Chemongok Hwanseon and Yongyeon which are located at Kangwon or chungcheongbook province in Korea and were identified to the genus level. Soil sam-ples were collected at 6 sites inside and 2 sites outside of each natural lime cave, As the result the strains belonging to genus Streptomyces and rare actinomycetes were isolated at the average of 2.1 and 3.4 strains per g soil on inside cave whereas which were isolated at the 6.0 and 1.8 strains per g soil on outside cave. How-ever the generic distribution of Streptomyces and rare actinomycetes isolated from outside cave was quite dif-ferent from that of inside cave. It was shown that rare actinomycetes at natural lime caves is generally highly abundant than Streptomyces.

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Degradation of Chlorinated Phenolic Compounds by Soil Actinomycetes Isolated from the Contami-nated Soil Nearby the Kyung-An River (경안천 유역 오염토양에서 분리한 방선균의 염화 페놀계 화합물 분해)

  • 김성민;김창영;김응수
    • Microbiology and Biotechnology Letters
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    • v.30 no.3
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    • pp.287-292
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    • 2002
  • Lignin-peroxidase (LiP) has been considered as one of the most important industrial enzymes for biodegradation of various recalcitrant toxic compounds such as chlorinated aromatic hydrocarbons and azo-dyes. Recently, several soil actinomycetes have been reported to secrete a functionally-similar lignin-peroxidase called actinomycetes lig-nin-peroxidase (ALiP). In this manuscript, we isolated over 100 morphologically distinct actinomycetes from the contaminated soils around 10 different gas stations located nearby the Kyung-An river. Among these actinomycetes screened based on the congo-red dye-decolorization activities, one newly-isolated actinomycetes named SMA-2 showed the most significant dye-decoloring activity on the congo-red plate as well as a significant ALiP activity in a yeast-extract-malt-extract liquid media supplemented with starch. The optimum SMA-2 culture condition fur ALiP production was determined and the kinetic parameters fur the SMA-2 AkIP activity were characterized. The optimally-cultured SMA-2 also exhibited the oxidation activities toward various recalcitrant aromatic compounds including phenol, 2- chlorophenol, 4- chlorophenol, 2,4- dichlorophenol ,2,6- dichlorophenol, and 2,4, f-trichlorophe - not, suggesting a potential application of SMA-2 for contaminated soil bioremediation.

Effect of Chemical Fertilizers and Organic Materials on Soil Actinomycetes Flora (화학비료(化學肥料) 및 유기물(有機物) 시용시(施用時) 방선균(放線菌) Flora의 구성변화(構成變化))

  • Hong, Sa-Hyun;Yang, Chang-Sool
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.4
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    • pp.420-426
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    • 1998
  • This investigation was undertaken to clarify the effects of application of chemical fertilizers (Compound fertilizer and commercial compost) and organic matters (straw and clover) on changes of the composition of soil actinomycetes flora. Actinomycetes were isolated from chemical fertilizer and organic materials treated soils, and then grown on HV agar plate. The isolated strains were classified by Bergey's manual based on the morphological characteristics and color of substrate mycelium of actinomycetes. The number of actinomycetes increased 4 times in control, 36 times in clover, 20 times in straw, 5 times in chemical fertilizer and 4 times in commercial compost treated soil after 14th day of incubation. This result suggests that the application of straw or clover is more effective to increase the number of actinomycetes than compound fertilizer or commercial compost. It also showed that the application of chemical fertilizer or organic materials considerably changed the composition of soil actinomycetes flora. The proportion of streptomyces strains to the isolated total actinomycetes was 62% in control, 60% in clover, 68% in straw, 67% in chemical fertilizer and 64% in commercial compost treated soils at the 14th day.

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Studies on Some Physiological and Morphological Characteristics of Actinomycetes in Paddy Soil applied Organic Materials (유기물(有機物) 시용시(施用時) 논 토양(土壤)에서 방선균(放線菌)의 형태(形態) 및 생리학적(生理學的) 특성(特性))

  • Park, Kyung-Soo;Park, Youl;Ryu, Jin-Chang
    • Korean Journal of Soil Science and Fertilizer
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    • v.20 no.1
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    • pp.77-84
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    • 1987
  • The population of actinomycetes in paddy soils, with hay compost and inorganic fertilizer had been applied respectively was investigated. Actinomycetes were isolated by using selective medium and the population densities of actinomycetes in paddy soil was examined. The population of actinomycetes were reached at a range from $2.1{\times}10^6$ to $7.4{\times}10^7$ per gram of the soil. The composition of actinomycetes flora changed considerably after hay compost applied. The significant positive correlations between the organic matter content in paddy soil and the actinomycetes populations were given at 1.38 to 2.69 level. According to the result of several morphological observation, similar strains isolated were classified into 21 groups. More detailed taxonomic characterization were carried out on the isolated strains. Therefore, 15 groups of Streptomyces and 6 groups of non-Streptomyces were classified into actinomycetes isolates, percentage of streptomyces and non-Streptomyces strains were 87.2% and 12.8% in the isolated 250 actinomycetes strains respectively. Streptomyces with sporophore of the spiral chain form accounted for 80% of all the Streptomyces isolates. Surface morphology of spores were determined with the electron microscope, three species have a spiny surface, and 13 strains have a smooth spore surface.

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Isolation and Identification of Methylotrophic Actinomycetes capable of Producing Anti-oral Cancer Activity (구강암에 대해 항암효과를 나타내는 methanol 자화 방선균의 분리 및 동정)

  • Kim, Jung;Kim, Sun-Sook
    • Journal of Korean society of Dental Hygiene
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    • v.1 no.2
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    • pp.193-200
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    • 2001
  • An appropriate amount of samples, collected from three each paddy forest, field and riverside soil near Taegu city, was suspended in sterile water and then diluted in order to isolation of antagonistic to oral cancer. The diluted samples were inoculated on separating medium in the routing spreading method. So, seven hundred and eighteen strains were isolated on HV agar and 220 strains were on methanol medium from soil samples. So, during the screening of anti-oral cancer activity from soil, we isolated microorganisms showing powerful antagonistic activity. Among them, No. 78 strain exhibited the most strongly anti-oral cancer activity. Microbiological properties were investigated by the methods described in the Bergey's Manual of Systematic Bacteriology and experimental methods of identification of actinomycetes by Hamada et al. As a result, a methylotrophic actinomycetes strain No. 79 was estimated as Amycolatopsis sp. based on taxonomic studies.

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An Improved Selective Isolation of Rare Actinomycetes from Forest Soil

  • Seong, Chi-Nam;Park, Ji-Heok;Baik, Keun-Shik
    • Journal of Microbiology
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    • v.39 no.1
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    • pp.17-23
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    • 2001
  • Various pretreatment procedures and selective media were applied to assess the optimal conditions for the isolation of rare actinomycetes from soil. Pretreatment of wet-heating for 15 min at 70$^{\circ}C$ and phenol treatment of soil suspension were the most effective methods for the isolation of these microorganisms. Hair hydrolysate vitamin agar (HHVA) was the most suitable medium for the recovery of rare actinomycetes. Thirty-five rare actinomycete strains were chosen using selective isolation approaches, then morphological and chemical properties of the isolates were determined. The isolates belonged to one of the following genus, Micromonospora, Microbispora, Actinoplanes and Streptosporangium.

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Distribution Pattern of Soil Actinomycetes on the Seasonal Change (계절에 따른 토양 방선균의 속 다양성 분포)

  • Park, Dong-Jin;Lee, Sang-Hwa;Park, Sang Ho;Kim, Chang-Jin
    • Korean Journal of Microbiology
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    • v.34 no.3
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    • pp.149-153
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    • 1998
  • From soils seasonally collected at two depths (0~2 cm, $50{\pm}1cm$) of forest, field, grass land, or paddy field, distinct strains of actinomycetes were isolated and identified to the genus level. The genus-diversity of soil actinomycetes was revealed to be considerably different by seasonal change. It was also affected by soil depths, soil types, or actinomycete groups. At the soil depth of 0~2 cm, the seasonal distribution fluctuation (%) of streptomycete strains was higher in grass land (41%), field (39%) soil than paddy field (18%), or forest (18%), whereas that of streptomycete strains at the soil depth of $50{\pm}1cm$ was high in order of paddy field (36%), field (28%), grass land (26%), and forest (16%). On the other hand, the seasonal distribution fluctuation ratio of rare actinomycete strains at the soil depth of 0~2 cm was above 45% except for paddy field (26%). At the soil depth of $50{\pm}1cm$, the seasonal distribution of rare actinomycete strains exhibited high fluctuation (%) in order of forest (79%), paddy field (36%), field (24%), and grass land (10%).

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Octimization of Score Production via Sonication of Antifungal Polyene-producing Actinomycetes (초음파 파쇄에 의한 항진균 폴리엔 생성 방선균의 포자형성 최적화)

  • Kim, Byung-Kyun;Han, Kyu-Beom;Kim, Eung-Soo
    • KSBB Journal
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    • v.22 no.4
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    • pp.218-221
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    • 2007
  • The polyene antifungal antibiotics, mostly produced by Gram-positive soil actinomycetes, are a family of type I polyketide macrolide ring compounds with 20$\sim$40 carbon backbone contain 3$\sim$8 conjugated double bonds. Using polyene-specific genomic screening strategy, we previously isolated three novel polyene-producing actinomycetes strains from soil, implying the potential application of these strains' spores as microbial pesticides. Here, we report that the sonication is a very efficient method for actinomycetes spore generation with a sonicator power-dependent manner. In addition, these sonication-driven actinomycetes spores retained significant portion of their cell viabilities as well as antifungal activities after freeze-drying procedure, implying the potential application of these strains' spores as microbial pesticides.

Isolation of $\alpha$-glucosiadase Inhibitor Producing Actinomycetes from Soil Sample (토양시료로부터 $\alpha$-glucosidase 저해제 생성 방선균의 분리)

  • 하남주;최성숙;정남용;김경제
    • Korean Journal of Microbiology
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    • v.38 no.2
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    • pp.139-143
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    • 2002
  • To find $\alpha$-glucosidase inhibitors produced by Actinomycetes, bacteria belonging to Actinomycetes were isolated from soil sample using Bennett's medium. The inhibitory activity induced by these bacteria on $\alpha$-glucosidase, which is the key enzymes far carbohydrates digestion and the prevention of diabetic complications, was investigated. A strain of these bacteria, PM718 potently inhibited $\alpha$-glucosidase activity in vitro.