• Title/Summary/Keyword: skin irritation

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Correlation Between Skin Irritation and Cytotoxicity of Anti-wrinkle Agents (화장품 원료의 피부자극성과 세포독성의 관련성)

  • 이은희;이종권;김용규;박기숙;안광수
    • YAKHAK HOEJI
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    • v.45 no.3
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    • pp.310-319
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    • 2001
  • To compare skin irritation and cytotoxicity of anti-wrinkle agents, we examined skin irritation of six anti-wrinkle agents (ascorbic acid, glycolic acid, all trans-retinoic acid, ginseng extract, retinol, EB) in New Zealand white rabbit. Cytotoxicity of these agents was determined by MTT [tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] at multi-time points in cultured HaCaT cell, a human immortalized keratinocyte cell. We then analyzed correlation between skin irritation and cytotoxicity by spearman's rank correlation analysis. All trans-retinoic acid showed the highest primary irritation index (0.92) in skin irritation test. Being all the six agents not irritant, retinal showed the most cytotoxic agents. The correlation between skin irritation and cytotoxicity ($IC_{50}$/ at different time point was 0.814, 0.757, 0.814 and 0.7 at 3, 24, 48 and 72 h, respectively. We also fecund that IC$_{20}$ and IC$_{80}$ of these agents showed similar correlation with skin irritation. These results therefore demonstrated that there is close correlation between skin irritation and cytotoxicity $IC_{50}$/ value by MTT in HaCaT cell at early time points by anti-wrinkle agents or IC$_{20}$ value. $IC_{50}$/ at earily time point or IC$_{20}$ values may be reliable alternative determinant of skin irritation.n.

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Skin Irriation Effect of Glycolic Acid and UVB in Guinea Pig (Guinea pid를 이용한 Glycolic acid 및 UVB의 피부 자극성 평가)

  • 조대현;홍진태
    • Toxicological Research
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    • v.16 no.1
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    • pp.89-94
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    • 2000
  • Alpha-hydroxy acid(AHA) are used in cosmetic products as a pH adjuster, mild exfoliant and humectant-skin conditioner. Cosmetics containing higher concentration (30%) and lower pH (3.0) of AHA can cause side effects if it is applied without the prescription. For providing information on the safety of AHA and on human risk assessments we studied skin irritation effect of glycolic acid, one of the most commonly used AHA in guinea pigs. The skin irritation by glycolic acid was increased in a dose(10% to 70%), acidity (pH 2.5 to 5.5.) and length of exposure dependent manner (for up to 14 days), respectively. The combination treatment with UVB (0.4 or 3.0 J/$cm^2$) increased glycolic acid-induced skin irritation. Histological examination showed that hyperplasia of non-inflammatory cells in the epidermis of skin treated with high dose of glycolic acid (pH 3.0). There results show that glycolic acid increased skin irritation in a dose, length of exposure and pH dependent manner, respectively, in guinea pig, and the combination with UVB increased glycolic acid-induced skin irritation. The cell proliferation of non-inflammatory cell may be involved in high doses of glycolic acid-induced skin irritation. Long-term application of more than 30% of glycolic acid (pH 3.0) may cause skin irritation.

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Studies on the Correlation between SPF index and Primary Irritation Index of Octylmethoxycinnamte (Octylmethoxycinnamate의 SPF 지수와 피부자극지수와의 상관성 연구)

  • 서수경;김종원;최주영;서경원;박창원;김규봉;김광진;이선희
    • Environmental Mutagens and Carcinogens
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    • v.22 no.4
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    • pp.296-302
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    • 2002
  • In recent years, the safety of sunscreens have been challenged based on the reports of its adverse effects on users; dermatitis, allergic contact dermatitis, photoallergic contact dermatitis. In the toxicological aspect, the need to investigate the safety of sunscreens is steadily increasing. However, there were few studies on the correlation between sun protection factor (SPF) and the safety of sunscreens. The objective of this study was to assess whether there was a correlation between SPF and the skin irritation index of sunscreens. Octyl methoxycinnamate (OMC) was selected, which was the most frequently used (about 92% in korea and about 90% in the world) in sunscreens. In vitro SPF test was performed using the standard samples recommended by KFDA guideline. We examined primary and cumulative skin irritation and eye irritation of OMC standard sample as well as homosalate. As in vitro SPF was increased, skin irritation index was increased in primary skin irritation test ($r^2$=0.986) and cumulative skin irritation test ($r^2$=0.939). In addition, OMC was non-irritating and non-sensitizing. In this results, there was a good correlation between SPF index and skin irritation index.

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Primary Skin Irritation tests for Sangmosu in Rabbits (토끼에 대한 Sangmosu의 국소독성 (일차 피부자극) 시험)

  • 최설민;김대영;박요안;김주영;김형식;안미영;곽승준;최이선;이병무
    • YAKHAK HOEJI
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    • v.43 no.4
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    • pp.542-545
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    • 1999
  • Primary skin irritation tests for Sangmosu were performed in male New Zealand White rabbits. In primary skin irritation test, body weights were not significantly changed and Primary Irritation Index(P.I.I) was 0.25, indicating Sangmosu as barely irritating material. These results indicate that Sangmosu was not considered to be irritant in test animals.

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A Study on Ocular and Skin Irritation Test of EPO(Erythropoietin) (토끼에서 EPO(Erythropoietin)의 안점막자극성 및 피부자극성시험)

  • 강병철;남정석;제정환;이석만;양재만;이학모;박재학;송동호;유선희
    • Toxicological Research
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    • v.13 no.1_2
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    • pp.149-152
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    • 1997
  • This test was performed to evaluate the ocular and skin irritation of EPO (Erythropoietin). The results as follows: 1. Ocular irritation test There were no observed clinical signs, body weght changes by EPO during experimental period. The acute ocular irritation index(A.O.I.), mean ocular irritation index(M.O.I.) and Day-7 individual ocular irritation index(I.O.I.) of EPO at dose of 1000U and 10, 000U were 0, respectively. Therefore we evaluated that EPO was non-toxic to eyes. 2. Skin irritation test There were no observed clinical signs, body weght changes and gross pathologic findings by EPO during experimental period. There were no observed erythema, eschar formation and edema formation on intact and abraded skin treated by EPO. The primary irritation index(P.I.I.) of EPO at dose of 1000U and 10, 000U were 0, respectively and were evaluated none irritating product about skin irritation.

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Comparison of Eye Irritation Potency with Skin Irritation and Cytotoxicity Potency of Anti-wrinkle Agents (주름방지용 화장품원료의 안점막 자극성과 피부자극성 및 세포독성과의 비교)

  • 이은희;이종권;김주환;정경미;정해관;이선희;정수연;홍진태
    • Toxicological Research
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    • v.17 no.2
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    • pp.143-149
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    • 2001
  • In the present study, we examined eye irritation oj six anti-wrinkle agents (ascorbic acid, glycolic acid, all trans-retinoic acid, ginseng extract, retinol, EB). We also compared eye irritation with skin irritation and cytotoxicity in HaCaT cells by these agents. The highest eye irritation was found in glycolic acid, but all trans-retinoic acid showed the highest skin irritation. The rank of eye irritation was not correlated with the cytotoxicity of agents. This result shows that eye irritation potency by these agents were not correlated with skin irritation potency, and cytotoxicity in HaCaT cells.

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Comparison of Skin Response Between New Zealand White Rabbit and Hartley Guinea Pig to Glycolic Acid (Glycolic Acid에 대한 토끼와 기니픽간의 피부자극성 비교)

  • 안광수;정경미;김용규;이종권;박기숙;김은정;이선희;홍진태
    • Toxicological Research
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    • v.17 no.1
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    • pp.59-63
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    • 2001
  • In this study, we compared skin response between Hartley guinea pig and New Zealand white rabbit. New Zealand white rabbit was treated by a glycolic acid (0, 8, 24, 40, 56 mg/$cm^2$) and UVB (0, 0.4, 3.0 J/$cm^2$) for 14 days. Skin irritation by glycolic acid and UVB were increased in dose and time-dependent manners, and the combination treatment of UVB increased glycolic acid-induced skin irritation. Comparison the skin irritation index between guinea pig and rabbit showed that guinea pig was much more sensitive to glycolic acid and UVB. This study indicated that selection of reliable species of animal could be considered in chemical-induced skin irritation study.

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Anti-inflammatory and Analgesic Activities, and Skin Irritation Test of Piroxicam Patch (피록시캄 패취제의 소염, 진통 효능 및 피부자극시험)

  • 고광호;조미정;이장훈;노민수;류재련;이진화;안재석;태주호;민동선
    • Biomolecules & Therapeutics
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    • v.2 no.1
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    • pp.71-76
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    • 1994
  • Anti-inflammatory and analgesic activities and skin irritation of piroxicam patch were investigated. Piroxicam patch increased the pain threshold in rat hind paw inflamed by carrageenan and inhibited writhing induced by acetic acid in mice. Piroxicam patch also inhibited the carrageenan-induced edema in rat hind paw as well as the increased vascular permeability induced by histamine in rats. In adjuvant arthritis of rats, piroxicam patch showed anti-inflammatory effects. Skin irritation of piroxicam patch was tested in Newzealand White rabbits and evaluated by Primary Irritation Index of Draize. The results from skin irritation test showed that piroxicam patch seemed practically non-irritating. The result from the present study indicates that piroxicam may be useful without serious side effects as anti-inflammatory analgesics in this patch form.

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Studies on the Correlation between SPF Index and Skin Irritation Index of Sunscreens (자외선 차단제의 SPF 지수와 피부자극지수와의 상관성 연구)

  • 김종원;서수경;최주영;박창원;서경원;김규봉;김광진;김재희;이선희
    • Biomolecules & Therapeutics
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    • v.10 no.4
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    • pp.246-252
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    • 2002
  • In recent years, the safety of sunscreens has been challenged based on the reports of their adverse effect on users; dermatitis, allergic contact dermatitis and photo allergic contact dermatitis. The unscientific idea that higher SPF sunsreen is good for health mealeads many users to tend to prefer higher SPF sunscreen. In the toxicological aspect, the need to investigate the safety of sunscreens is steadily increasing. However, there were few studies on the correlation between sun protection factor (SPF) and the safety of sunscreens. The objective of this study was to assess whether there was a correlation between SPF and the safety of sunscreens. We measured in vitro SPF index using homosalate as a standard and examined the toxicity tests such as primary skin irritation tests, ocular irritation test and skin sensitization test. Homosalate (HS), octyl methoxycinnamate (OMC), octyl salicylate (OS), octocrylene (OC) as UVB organic filter and benzophenone-3 (BP3), butyl methoxy dibenzoil methane (BMDM) as UVA organic filter, and titanium dioxide (TD), zinc oxide (ZO) as inorganic filters were used. The skin irritation indexes in rabbits treated with HS, OMC, OS, BP3, and BMDM were significantly increased as SPF indices were increased. Neither ocular irritation in rabbits nor skin sensitization in guinea pigs were increased. It suggests that there might be a good correlation between SPF and the skin irritation indices of organic UV filters and skin irritation might be one of most sensitive index to assess the safety of sunscreens.

Mixture Toxicity of Methylisothiazolinone and Propylene Glycol at a Maximum Concentration for Personal Care Products

  • Park, Juyoung;Lee, Handule;Park, Kwangsik
    • Toxicological Research
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    • v.34 no.4
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    • pp.355-361
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    • 2018
  • Methylisothiazolinone (MIT) has been used in combination with methylchloroisothiazolinone (CMIT) for cosmetic products such as shampoo, body lotion, and skin care products. The mixture of CMIT/MIT has been found to cause allergic contact dermatitis and is thus no longer permitted for use as a preservative in leave-on cosmetics. However, MIT itself was approved as a stand-alone preservative at a maximum concentration of 100 ppm as the toxicity was derived from CMIT rather than MIT. However, in many countries, allergic skin irritation caused by MIT remains a social concern. In this study, skin irritation was assessed for the presence of MIT, propylene glycol, and their mixture using a 3D human skin model $EpiDerm^{TM}$. Although non-diluted MIT causes serious skin toxicity, skin irritation was not observed at a concentration of 100 ppm, the maximum permissible level for cosmetics and personal care products according to European regulations. Propylene glycol, the most widely used vehicle for MIT, did not cause skin irritation in the 3D skin model. The results are expected to provide information for regulatory policies and guidelines on the use of biocides in consumer products.