• Title, Summary, Keyword: single-node culture

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Influences by position of node and existence of leaf on microtuberization in node culture of potato (감자절간배양에서 마디위치 및 잎의 부착유무에 따른 소괴경형성의 차이)

  • Hwang, Hye-Yeon;Lee, Young-Bok
    • Journal of Plant Biotechnology
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    • v.35 no.1
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    • pp.63-68
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    • 2008
  • Single-node stem pieces ca. 1 cm in length containing a axillary bud were obtained from in vitro plants of potato (Solanum tuberosum L.). The influences by a position of the node and the existence of a leaf at the node were observed in the single-node culture on the 8% sucrose MS medium. The effect of CCC was also investigated for the microtuberization. The apical part node was excellent in the tuberization not to mention shoot length, fresh weight, diameter, the number of node on the in vitro culture of a single-node than the lower part. The differences in the diameter of a tuber formed in the part of the axillary bud on all treatments including the cultivation of the apical part node were not recognized. However, the fresh weight of the tuber showed high value in the tuber formed at the axillary bud of shoot apex part. At 20 days after cultivation, tuberization was promoted in the new stolen that developed from the bud of node with a leaf under SD condition of 8 hours at $20^{\circ}C$. The tuberization from axillary bud of the single-node without leaf was inhibited at high temperature of $28^{\circ}C$ regardless of daylength. Whereas, tuberization at $20^{\circ}C$ and $28^{\circ}C$ was similar without the difference under SD condition but the tuber formation ratio were low. CCC 500 mg/L promoted tuberization and the effect was also showed even under LD condition at $28^{\circ}C$. The inhibiton of tuberization under LD and high temperature condition could be solved by treatment with CCC.

Effects of Cultural Conditions on the In Vitro Propagation of Plantlets derived from Apical Meristem in Ipomoea batatas L. (고구마 정단분열조직 유래 식물체의 기내 증식에 미치는 배양조건의 영향)

  • Eun, Jong-Seon;Park, Jong-Suk;Kim, Young-Seon
    • Journal of Plant Biotechnology
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    • v.29 no.1
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    • pp.37-40
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    • 2002
  • Sweet potato is a crop vegetatively propagated by vine cuttings, an ineffective method for maintaining pathogene-free stock plants. As an alternative method, single-node cultures of virus-free plantlets derived from apical meristem in sweet potato (cv. Yulmi) was examined. Effective pH range, sugar concentration and nodal order were investigated to establish an in vitro mass propagation system with high quality virus-free stock plantlets to farmhouse. Although the plantlets grew at wide range of pH, the most effective pH of the medium was 4.8 in single-node cultures. High sugar concentration of 60∼80 g/L resulted in increased growth response in shoot length, root length, number of node, leaf area and fresh and dry weight of shoot and root, whereas reducing sugar contents below 6% was showed reduced growth response. The first node including meristem tip was the best for the rapid growth of plantlets and the other nodes also showed a very similar growth response. Uniform plantlet can be obtained massively at the same time by culture of single node except for the first node including meristem tip. In conclusion, the most effective pH range and sugar concentration of medium for the growth of plantlets via single-node cultures was 4.8, 60∼80 g/L respectively. The first node was the best for the rapid propagation of plantlets in nodal order.

In Vitro Regeneration of Pongamia pinnata Pierre

  • Sujatha, K.;Hazra, Sulekha
    • Journal of Plant Biotechnology
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    • v.33 no.4
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    • pp.263-270
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    • 2006
  • Pongamia pinnata Pierre is a tree legume, having potential in production of raw material for biodiesel. A protocol for in wk propagation of this plant was standardized using seedling explants. Growth regulators (GR) including gibberellic acid $(GA_3),\;N^6-benzylaminopurine(BA)$, thidiazuron (TDZ), and Adenine sulphate (Ads) were tested for optimum germination of seeds. Removal of seed coat prior to germination, controlled fungal growth partially but enhanced bacterial growth. Antibiotic cefotaxime was ineffective in controlling bacterial contamination. Seedling derived nodal explants and cotyledon nodes with attached cotyledons were excised and cultured for induction of shoots. Optimum sprouting and multiplication of shoot buds were obtained in MS medium supplemented with $8.88{\mu}M$ BA. These buds differentiated and rooted on medium devoid of GR. Optimum growth of Pongamia seedling was obtained in cotton plugged culture vessels. Reculturing of the cotyledon node explants produced more shoots from the same site. This process of removing shoots and reculturing of cotyledon node was followed for eight passages yielding 4 to 8 shoots in each cycle. The shoots (75%) rooted on half strength MS basal medium supplemented with 0.22% charcoal. All plants survived on transfer to soil. This is the first report on in vitro regeneration of Pongamia pinnata. This report demonstrates the possibility of coupling more than one parameter in single experiment to hasten the process of standardization. The process of cycling the nodal explant repeatedly for production of large number of shoots from single meristem may find application in genetic transformation experiments wherein meristems are used for transformation.

An Efficient Routing Protocol for Mobile Sinks in Sensor Networks (센서 네트워크에서 모바일 싱크를 위한 효율적인 라우팅 기법)

  • Wu, Mary
    • Journal of Korea Multimedia Society
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    • v.20 no.4
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    • pp.640-648
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    • 2017
  • Sensors have limited resources in sensor networks, so efficient use of energy is important. In order to use the energy of the sensor node efficiently, researches applying mobile sink to the sensor network have been actively carried out. The sink node moves the sensor network, collects data from each sensor node, which spread the energy concentrated around the sink node, thereby extending the entire life cycle of the network. But, when the sink node moves, it requires a reset of the data transmission path, which causes a lot of control messages and delays. In this paper, we propose a CMS(Cluster-based Mobile Sink) method to support the movement of mobile sink in a cluster sensor environment. The proposed scheme minimizes an amount of control messages without resetting the routing paths of entire sensor networks by supporting the sink mobility path using the neighbor cluster list. And, it simplifies the routing path setup process by setting a single hop path between clusters without a gateway. The experiment results show that the proposed scheme has superior energy efficiency in processing and network structure, compared with existing clustering and mesh routing protocols.

Dual-hop Routing Protocol for Improvement of Energy Consumption in Layered WSN Sensor Field

  • Song, Young-Il;LEE, WooSuk;Kwon, Oh Seok;Jung, KyeDong;Lee, Jong-Yong
    • International Journal of Advanced Culture Technology
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    • v.4 no.2
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    • pp.27-33
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    • 2016
  • This paper proposes to increase the node energy efficiency, which rapidly drops during the transmission of L-TEEN (Layered Threshold sensitive Energy Efficient sensor Network protocol), using the method of DL-TEEN (Dual-hop Layered TEEN). By introducing dual-hop method in the data transmission, the proposed single-hop method for short-range transmission and multi-hop transmission method between the cluster heads for remote transmission was introduce. By introducing a partial multi-hop method in the data transmission, a single-hop method for short range transmission method between the cluster heads for remote transmission was introduces. In the proposed DL-TEEN, the energy consumption of cluster head for remote transmission reduces and increases the energy efficiency of sensor node by reducing the transmission distance and simplifying the transmission routine for short-range transmission. As compared the general L-TEEN, it was adapted to a wider sensor field.

Effect of Mineral Nutrient Control on Nutrient Uptake, Growth and Yield of Single-Node Cutting Rose Grown in a Closed Hydroponic System (순환식 수경재배시 무기이온 조절이 Single-Node Cutting 장미의 양분흡수, 생육 및 품질에 미치는 영향)

  • Yang, Eun-Young;Park, Keum-Soon;Oh, Jeong-Sim;Lee, Hye-Jin;Lee, Yong-Beom
    • Protected Horticulture and Plant Factory
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    • v.17 no.4
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    • pp.252-260
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    • 2008
  • This study was conducted to observe the characteristics of mineral nutrient uptake of single-node cutting rose 'Versilla' and to determine optimal nutrient solution control method for soilless culture of 'Versilla' in a closed hydroponic system. Nutrient solution was managed by five different control methods: macro- and micro-element control in aeroponic system (M&M), macroelement control in aeroponic system (M), nutrient solution supplement in aeroponic system (S); electrical conductivity (EC) control in aeroponic system(EC-A); EC control in deep flow technique system(EC-D). The concentration of $NO_3$-N exceeds optimal range whereas P and Mg decreased at the later stage of plant growth with the EC control method, EC-A and EC-D. The overall mineral nutrient content increased with S. On the other hand the nutrient content at the root environment was maintained optimal with M&M and M. The nutrient solution control methods had significantly effect on the cut-flower quality. In the M&M and M, flower length, fresh weight and root activity were higher than those with the other mineral nutrients control methods. The maximal efficiency of photochemistry (Fv/Fm) was higher for M&M, M and S than that with EC-A and EC-D. Based on the above results, it is highly recommended to control nutrient solution by mineral nutrient control methods (M&M and M) in a closed hydroponic system for single-node cutting rose, 'Versillia'.

Identification of DNA Variations Using AFLP and SSR Markers in Soybean Somaclonal Variants

  • Lee, Suk-Ha;Jung, Hyun-Soo;Kyujung Van;Kim, Moon-Young
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.1
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    • pp.69-72
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    • 2004
  • Somaclonal variation, defined as phenotypic and genetic variations among regenerated plants from a parental plant, could be caused by changes in chromosome structure, single gene mutation, cytoplasm genetic mutation, insertion of transposable elements, and DNA methylation during plant regeneration. The objective of this study was to evaluate DNA variations among somaclonal variants from the cotyledonary node culture in soybean. A total of 61 soybean somaclones including seven $\textrm{R}_1$ lines and seven $\textrm{R}_2$ lines from Iksannamulkong as well as 27 $\textrm{R}_1$ lines and 20 $\textrm{R}_2$ lines from Jinju 1 were regenerated by organogenesis from the soybean cotyledonary node culture system. Field evaluation revealed no phenotypic difference in major agronomic traits between somaclonal variants and their wild types. AFLP and SSR analyses were performed to detect variations at the DNA level among somaclonal variants of two varieties. Based on AFLP analysis using 36 primer sets, 17 of 892 bands were polymorphic between Iksannamulkong and its somaclonal variants and 11 of 887 bands were polymorphic between Jinju 1 and its somaclonal variants, indicating the presence of DNA sequence change during plant regeneration. Using 36 SSR markers, two polymorphic SSR markers were detected between Iksannamulkong and its somaclonal variants. Sequence comparison amplified with the primers flanking Satt545 showed four additional stretches of ATT repeat in the variant. This suggests that variation at the DNA level between somaclonal variants and their wild types could provide basis for inducing mutation via plant regeneration and broadening crop genetic diversity.

Effect of Growth Regulator, Sucrose, and Minimal-growth Conservation on In Vitro Propagation of Virus-free Sweet Potato Plantlets (고구마 무병묘의 기내 증식에 미치는 생장조절물질, Sucrose, 최소생장 보존의 영향)

  • Lee, Na Rha;Lee, Seung Yeob
    • Protected Horticulture and Plant Factory
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    • v.29 no.1
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    • pp.1-8
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    • 2020
  • The influence of growth regulators (NAA and BA) and sucrose concentrations (0, 3, 5, 7, 9%) on in vitro rapid-propagation of virus-free sweet potato [Ipomoea batatas (L.) Lam.] was investigated with single-node or shoot-tip culture of two cultivars ('Matnami' and 'Shinhwangmi'). The survival rate and growth of shoot-tip explant was also investigated under the presence or absence of light (blue and red LED = 7:3, 150±5 μmol·m-2·s-1 PPFD) during minimal-growth in vitro conservation at 15℃. Vine length, vine diameter, fresh weight and dry weight were enhanced without callusing of explant in the MS medium supplemented with 0.2-0.5 mg·L-1 BA. The growth of single-node and shoot-tip explants were significantly enhanced with the increase of vine length, number of leaf, number of root, fresh weight, and dry weight in the solid medium containing 5% sucrose and 0.2 mg·L-1 BA. Vine elongation of shoot-tip explants were highest in the liquid medium containing 3% sucrose than the solid medium. The survival rate of minimal-growth in vitro conservation was 100% in 5 months under the presence of light (LED, 150±5 μmol·m-2·s-1 PPFD) at 15℃, but the explants in dark condition died in 3 months. The light was absolutely necessary for the in vitro conservation under minimal-growth conditions of virus-free sweet potato plantlets at 15℃, and the high density of explants (10 plantlets per Petri Dish) was increased the efficiency of mass conservation.

DL-LEACH: Hierarchical Dual-Hop Routing Protocol for Wireless Sensor Network (DL-LEACH : 무선 센서 네트워크를 위한 계층형 멀티 홉 라우팅 프로토콜)

  • Lee, Chang-Hee;Lee, Jong-Yong
    • The Journal of The Institute of Internet, Broadcasting and Communication
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    • v.15 no.5
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    • pp.139-145
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    • 2015
  • This paper proposes to increase the node energy effienciecy, which rapidly drops during the transmission of LEACH (Low Energy Adaptive Clustering Hierachy), using the method of DL-LEACH (Dual-hop Layered LEACH). By introducing dual-hop method in the data transmission, the proposed single-hop method for short-range transmission and multi-hop transmission method between the cluster heads for remote transmission was introduce. By introducing a partial multi-hop method in the data transmission, a single-hop method for short range transmission method between the cluster heads for remote transmission was introduces. In the proposed DL-LEACH, the energy consumption of cluster head for remote transmission reduces and increases the energy efficiency of sensor node by reducing the transmission distance and simplifying the transmission routine for short-range transmission. As compared the general LEACH, it was adapted to a wider sensor field.

In vitro Conservation of Coleus forskohlii- an Endangered Medicinal Plant

  • Rajasekharan P.E.;Ambika S.R.;Ganeshan S.
    • Journal of Plant Biotechnology
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    • v.7 no.2
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    • pp.135-141
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    • 2005
  • Protocols for in vitro conservation was developed for Coleus forskohlii. Plants maintained both in field served as explant source. Shoot tips and single node cuttings were used to optimize protocols for in vitro multiplication. MS basal medium supplemented with $0.54\;{\mu}M$ naphthalene acetic acid (NAA) and $8.87\;{\mu}M$ benzy-ladenine (BA) induced multiple shoots in shoot tips and nodes. Shoot multiplication was amplified with a gradual decrease of BA concentration, leading to its final omission after 4 months. Concomitant rooting on multiplication media enabled successful establishment extra vitrum. For in vitro conservation studies, experiments were carried out with 2-3 week maintained in vitro plants under standard and reduced culture conditions (SCC, RCC). In vitro plants could be successfully conserved in full strength MS medium (FMS) under SCC for 6 months without subculture with full potential to regenerate, producing viable shoots and nodes. The root production remained unaffected due to conservation, showing high rooting activity in mannitol and low temperature treatments. Preset low temperature (15 and $10^{\circ}C$) and reduction in media constituents does not appear to favour conservation, although the former accomplished conservation levels equal to (FMS) under SCC.