• Title, Summary, Keyword: rosmarinic acid

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Anti-Atherosclerosis Activity of Rosmarinic Acid in Human Aortic Smooth Muscle Cells (사람 동맥 평활근 세포에 대한 Rosmarinic Acid의 항동맥경화 활성)

  • Ha, Jung-Jae;Yun, Hyun-Jeong;Huh, Joon-Young;Kim, Jai-Eun;Park, Sun-Dong
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.6
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    • pp.1423-1430
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    • 2009
  • Rosmarinic acid frequently found as a secondary metabolite in herbs and medicinal plants, has exhibited antimicrobial, antiviral, antioxidative, and anti-inflammatory activities. The proliferation and migration of human aortic smooth muscle cells (HASMC) in response to activation by various stimuli plays a critical role in the initiation and development of atherosclerosis. This study was conducted to examine the effects of Rosmarinic acid on the proliferation and migration of HASMC. Rosmarinic acid suppressed the proliferation of HASMC via induction of the expression of apoptotic proteins including cleaved poly ADP-ribose polymerase (PARP), and caspase-3. Rosmarinic acid decreased anti-apoptotic Bcl-2 and increased pro-apoptotic Bax. Moreover, treatment of rosmarinic acid decreased the G1/S cycle regulation proteins (cyclin D1, cyclin E, CDK2, CDK4 and CDK6) and increased p21, p27 and p53. Rosmarinic acid also blocked HASMC migration via suppression of MMP-9 and MMP-2. Taken together, these results indicate that rosmarinic acid has the potential for use as an anti-atherosclerosis agent.

Influence of Different Strains of Agrobacterium rhizogenes on Hairy Root Induction and Rosmarinic Acid Production in Agastache rugosa Kuntze (Agrobacterium rhizogenes strains이 배초향 모상근 유도와 Rosmarinic acid 생산에 미치는 영향)

  • Kim, Jong-Se;Oh, Eun-Jeong;Lee, Sook-Young
    • Korean Journal of Plant Resources
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    • v.23 no.1
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    • pp.14-18
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    • 2010
  • Rosmarinic acid, an ester of caffeic acid with 3,4-dihydroxyphenyl lactic acid, is one of the main active constituents of Agastache rugosa Kuntze and has an astringent property, antioxidant capacity, anti-inflammatory activity, antimutagenic ability, antimicrobial capacity, and an antiviral property. Five different strains of Agrobacterium rhizogenes differed in their ability to induce Korean mint (Agastache rugosa Kuntze) hairy roots and also showed varying effects on the growth and rosmarinic acid production in hairy root cultures. A. rhizogenes R1601 is the most effective strain for the induction (72.90%), growth (13.50 g/l) and rosmarinic acid production (22.60 mg/g) in hairy root of Korean mint. Our results demonstrate that use of suitable strains of A. rhizogenes may allow study of the regulation of rosmarinic acid biosynthesis in hairy root cultures of Agastache rugosa.

The Protective Effect of Rosmarinic Acid on the Aluminum of Dementia Inducer (치매유발제인 알루미늄에 대한 Rosmarinic Acid의 보호 효과)

  • Jung, In-Ju;Seo, Young-Mi;Jekal, Seung-Joo
    • Korean Journal of Clinical Laboratory Science
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    • v.49 no.1
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    • pp.8-14
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    • 2017
  • To examine the protective effect of rosmarinic acid on the aluminum of dementia inducer, cultured C6 glioma cells were treated with various concentrations of aluminum chloride ($AlCl_3$) or rosmarinic acid. The cell viability, electron donating ability (EDA), superoxide dismutase (SOD)-like activity, and inhibitory activity of lipid peroxidation were evaluated for the antioxidant effect of rosmarinic acid. In these cultures, $AlCl_3$ sowed a cytotoxic effect by decreasing the cell viability in a dose-dependent manner; then, the $XTT_{50}$ value was measured at $142.2{\mu}M$ of $AlCl_3$ after treating the cultured C6 glioma cells with media containing $120{\sim}160{\mu}M\;AlCl_3$. Therefore, its toxicity was determined as mid-cytotoxic by Borenfreund and Puerner's toxic criteria; while, vitamin E of antioxidant markedly increased the cell viability on $AlCl_3$-induced cytotoxicity in these cultures. This study showed the antioxidant effect of rosmarinic acid via several assays, such as electron donating activity (EDA), superoxide dismutase (SOD)-like activity, and inhibitory activity of lipid peroxidation. From these findings, it is suggested that the oxidative stress is involved in $AlCl_3$-induced cytotoxicity, and rosmarinic acid was effective in the protection of $AlCl_3$-induced cytotoxicity by antioxidant activity. In conclusion, natural resources, like rosmarinic acid, may be a putative antioxidant agent for the treatment of reactive oxygen species (ROS)-mediated disease, such as dementia.

The Neuro-Protective Effect of the Methanolic Extract of Perilla frutescens var. japonica and Rosmarinic Acid against H2O2-Induced Oxidative Stress in C6 Glial Cells

  • Lee, Ah Young;Wu, Ting Ting;Hwang, Bo Ra;Lee, Jaemin;Lee, Myoung-Hee;Lee, Sanghyun;Cho, Eun Ju
    • Biomolecules & Therapeutics
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    • v.24 no.3
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    • pp.338-345
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    • 2016
  • Neurodegenerative diseases are often associated with oxidative damage in neuronal cells. This study was conducted to investigate the neuro-protective effect of methanolic (MeOH) extract of Perilla frutescens var. japonica and its one of the major compounds, rosmarinic acid, under oxidative stress induced by hydrogen peroxide ($H_2O_2$) in C6 glial cells. Exposure of C6 glial cells to $H_2O_2$ enhanced oxidative damage as measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and thiobarbituric acid-reactive substance assays. The MeOH extract and rosmarinic acid prevented oxidative stress by increasing cell viability and inhibiting cellular lipid peroxidation. In addition, the MeOH extract and rosmarinic acid reduced $H_2O_2-indcued$ expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the transcriptional level. Moreover, iNOS and COX-2 protein expression was down-regulated in $H_2O_2-indcued$ C6 glial cells treated with the MeOH extract and rosmarinic acid. These findings suggest that P. frutescens var. japonica and rosmarinic acid could prevent the progression of neurodegenerative diseases through attenuation of neuronal oxidative stress.

In Vitro Anticomplementary Activity of Phenylpropanoids from Agastache rugosa

  • Oh, Sei-Ryang;Jung, Keun Young;Lee, Hyeong-Kyu
    • Korean Journal of Pharmacognosy
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    • v.27 no.1
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    • pp.20-25
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    • 1996
  • In searching for anticomplementary compounds, three phenylpropanoids were isolated from the roots of Agastache rugosa and identified as rosmarinic acid (RA), rosmarinic acid methyl ester (RAM) and caffeic acid methyl ester (CAM) by NMR analyses. RA and RAM exhibited strong inhibitory activity on both the classical pathway (CP) and the alternative pathway (AP) of the complement system, in vitro, but CAM did far less than RA and RAM. $RAM-M1{\sim}-M5$, the methylated derivatives from the RAM, showed that the inhibitory activity was decreased in inverse proportion to the number of methylated groups and $RAM-M 2{\sim}-M4$, the isomers of two methylated hydroxyl groups, exhibited different inhibition activity.

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Variation of Caffeic acid, Rosmarinic acid, Luteolin and Apigenin Contents in Perilla Germplasm

  • Lee, Myoung-Hee;Jung, Chan-Sik;Pae, Suk-Bok;Hwang, Chung-Dong;Park, Chang-Hwan;Shim, Kang-Bo;Park, Keum-Yong;Kim, Hyun-Kyung;Park, Soon-Ki;Ha, Tae-Joung
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.391-396
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    • 2009
  • Perilla frutescens, which belonging to the Labiatae family, is widely cultivated oil crop and have been used traditional herbal medicine in East Asia such as Korea, China, and Japan. Especially, the leaves and the seeds of this species are important in Korean traditional cooking, as one of the popular garnish and food colorants. Numerous studies have revealed that the beneficial health effects of perilla are due to its several phytochemicals contents, such as rosmarinic acid, caffeic acid, luteolin, and apigenin. For this reason, increasing the content of phytochemicals in perilla hasbecome a major breeding objective. The genetic diversity of the rosmarinic acid, caffeic acid, luteolin, and apigenin content in perilla seed is poorly documented. We analyzed the rosmarinic acid, caffeic acid, luteolin, and apigenin content of 203 accessions of perilla germplasm by high performance liquid chromatography (HPLC). The rosmarinic acid and luteolin contents ofgermplasms were ranged from $15.7{\mu}g/g$ to $2717.1{\mu}g/g$ and from $1.6{\mu}g/g$ to $582.4{\mu}g/g$ respectively.

Rosmarinic Acid as a Tyrosinase Inhibitors from Salvia miltiorrhiza

  • Kang, Hye-Sook;Kim, Hyung-Rak;Byun, Dae-Seok;Park, Hye-Jin;Choi, Jae-Sue
    • Natural Product Sciences
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    • v.10 no.2
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    • pp.80-84
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    • 2004
  • Rosmarinic acid and its methyl ester, isolated from the ethyl acetate soluble fraction of the methanolic extract of Salvia miltiorrhiza Bunge (Labiatae), were found to inhibit the oxidation of L-tyrosine catalyzed by mushroom tyrosinase with the $IC_{50}$ values of 16.8 and $21.5\;{\mu}M$, respectively. It was comparable with kojic acid, a well-known tyrosinase inhibitor, with an $IC_{50}$ of $22.4\;{\mu}M$. The inhibitory kinetics analyzed by the Lineweaver-Burk plots, were found rosmarinic acid and its methyl ester to be competitive inhibitors with $K_i\;of\;2.4{\times}10^{-5}\;and\;1.5{\times}10^{-5}\;M$, respectively.

Active Ingredients and Antioxidant Activities of Salvia plebeia R. Br. According to Pretreatment Conditions (전처리 조건에 따른 배암차즈기(Salvia plebeia R. Br.)의 주요 성분의 함량 및 항산화)

  • Kim, Yong-Joo;Jeong, Ji-Suk;Park, No-Jin;Go, Geun-Bae;Son, Byeong-Gil
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.12
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    • pp.1948-1953
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    • 2014
  • This study examined changes in antioxidant activity as well as contents of rosmarinic acid, homoplantaginin, and luteolin, which is the main substance of Salvia plebeia R. Br. (SPR) known to have anti-inflammatory efficacy, according to drying, blanching, and fermentation conditions. Rosmarinic acid content was 16.42 mg/g upon hot-air drying and 10.19 mg/g upon hot-air drying after blanching, and there was no significant difference in the case of leaf and root freezing or cold-air drying. Rosmarinic acid content was 8.69 and 8.15 mg/g in the case of air-drying in the shade and freeze-drying, respectively, and decreased to 0.05 mg/g or undetected after fermentation. SPR processed by freeze-drying, cold-air drying in the shade, and hot-air drying showed ABTS radical scavenging ability over 98.5% at a concentration over $500{\mu}g/mL$ as well as excellent radical scavenging ability of 87.3% in the case of hot-air drying after blanching. Root showed lower ABTS radical scavenging ability than leaves. SOD-like activity was measured to be 6.1~27.8% at a concentration of $1,000{\mu}g/mL$, which was significantly difference from ABTS radical scavenging ability. As rosmarinic acid and homoplantagine, an anti-inflammatory material contained in SPR, are almost undetectable after oxidation fermentation during processing, hot-air drying after blanching or drying seems to be suitable to develop SPR as a functional substance.

Extraction of Caffeic Acid and Rosmarinic Acid from Zostera marina Based on Ionic Liquids and Deep Eutectic Solvent (이온성액체와 Deep Eutectic Solvent를 사용한 잘피에서 카페인산과 로즈마리산의 추출)

  • Lee, Yu Ri;Lee, Yu Jin;Row, Kyung Ho
    • Korean Chemical Engineering Research
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    • v.52 no.4
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    • pp.481-485
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    • 2014
  • The applicability of the dipping, ultrasonic-assisted, heating methods to the extraction of useful components from Zostera marina was investigated. For the increase of the extraction yield of caffeic acid and rosmarinic acid from Zostera marina, ionic liquids and DES were used as additives in the extraction solvent. The optimum extraction conditions were found: dipping extraction, methanol as extraction solvent, 0.005 g of freeze-dried material powder, 5 mL methanol, 30 min and 0.5 g of DES-3 ($Et_4NCl$ and phenol) as additive. As a result, 0.19 mg/g of caffeic acid and 8.48 mg/g of rosmarinic acid were obtained. This method is simple and sensitive, and has been applied successfully to determine the component of caffeic acid and rosmarinic acid in Zostera marina and these results indicate that DES were used as additives is more suitable than traditional extraction for the extraction of useful components from Zostera marina.

Tyrosinase Inhibiting and DPPH Radical Scavenging Activities of Rosmarinic Acid and Its Methyl ester from Salvia miltiorrhiza

  • Kang, Hye-Sook;Kim, Hyeung-Rak;Chung, Hae-Young;Choi, Jae-Sue
    • Proceedings of the PSK Conference
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    • pp.383.3-384
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    • 2002
  • Rosmarinic acid (1) and methyl rosmarinic acid (2), isolated from the ethyl acetate soluble fraction of the methanolic extract of Salvia miltiorrhiza Bunge (Lamiaceae) were found to be the tyrosinase inhibitors and scavengers of 1, 1-diphenyl-2-picrylhydrzyl (DPPH) radical. Compounds 1 and 2 inhibited the oxidation of L-tyrosine catalyzed by mushroom tyrosinase with $IC_{50}$/ of 16.8 $\mu\textrm{M}$ and 21.5 $\mu\textrm{M}$. respectively. It compared well with kojic acid. a well-known tyrosinase inhibitor. with an $IC_{50}$ of 22.4 $\mu\textrm{M}$. The inhibitory kinetics, analyzed by a Lineweaver-Burk plot, found rosmarinic acid and its methyl ester to be competitive inhibitors with $K_{i}$ of $2.35{\times}10^{-5}M$ and $1.52{\times}10^{-5}M$ respectively. In addition, compounds 1 and 2 showed the scavenging activities on DPPH radical, with $IC_{50}$ of 4.27 $\mu\textrm{M}$ and 3.05 $\mu\textrm{M}$. respectively. These scavenging effects were more potent than that of L-ascorbic acid ($IC_{50}$ = 11.75$\mu\textrm{M}$).

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