• Title, Summary, Keyword: rice(Oryza sativa L.)

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Gene flow from herbicide resistant genetically modified rice to conventional rice (Oryza sativa L.) cultivars

  • Han, Sung Min;Lee, Bumkyu;Won, Ok Jae;Hwang, Ki Seon;Suh, Su Jeoung;Kim, Chang-Gi;Park, Kee Woong
    • Journal of Ecology and Environment
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    • v.38 no.4
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    • pp.397-403
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    • 2015
  • Rice (Oryza sativa L.) is an important feeding crop in Asia, and utilization of genetically modified (GM) rice is highly demanding. For co-existence of GM rice and non-GM rice, the proper confinement measures should be provided. Thus, we surveyed gene flow from herbicide resistant GM rice to the conventional rice cultivars in the field tests. Gene flow frequency decreased with increasing distance between the pollen donor and recipients and did not exceed more than 1% even at the nearest distance. In single recipient model plot, a maximum gene flow frequency was observed at the shortest distance and hybrid was detected up to 12 m from the pollen donor. The direction of gene was coincided with the dominant wind direction. Gene flow assessment to multiple recipient plots was conducted under the high raining season by chance, and abrupt decline of gene flow frequency and maximum distance were resulted. According to the survey results, current regulation for isolation distance is reasonable for environmental safety or for general crop production. However, we suggest an alternative measure for GM rice cultivation that should be supplemented to overcome the out of estimation and in the environment asking higher security levels.

Morphological Characters of Panicle and Seed Mutants of Rice

  • Kang Sang-Gu;Pandeya Devendra;Kim Sung-Soo;Suh Hak-Soo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.4
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    • pp.348-355
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    • 2006
  • Phenotypes of panicle, hull and seed of mutant rice (Oryza sativa L.) were characterized. Panicle mutants were classified in 4 groups with their internode length of main rachis, primary rachis, secondary rachis and pedicel. Hull and seed mutants were grouped into 12 based on their mutant characters in shape, size and color of seeds. These natural and spontaneous mutant collections showed distinct phenotypes to wild type rice. This might be useful for the identification of the functions of genetic factors in the Mendelian inheritance.

The Effect of Ginseng Saponin Fraction on the Germination and Early Growth of Rice Seed (Oryza sativa L.) (인삼사포닌이 벼의 발아 및 초기 생장에 미치는 영향)

  • 이용우;주충노
    • Journal of Ginseng Research
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    • v.11 no.1
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    • pp.77-83
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    • 1987
  • The effects of ginseng saponin on the germination and early growth of rice seeds (Oryza sativa L.) were investigated. The early growth (length) of test rice seeds which were rinsed for 60 hrs in $10^{-4}$% saponin solution prior to transplantation to water agar bed was about 20% faster than that of control seeds, It was also found that the amylase activity of the seeds was most active when the seeds were rinsed in 10-4% saponin solution for 48 hrs. In vitro investigation showed that the amylase activities were stimulated about 30% by the treatment of $10^{-5}$% saponin solution compared with control group. From the above results, it seems that the ginseng saponin might activate amylase of rice seed during germination, resulting in rapid growth of rice.

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Telomere association of Oryza sativa telomere repeat-binding factor like 1 and its roles in telomere maintenance and development in rice, Oryza sativa L.

  • Byun, Mi Young;Cui, Li Hua;Lee, Hyoungseok;Kim, Woo Taek
    • BMB Reports
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    • v.51 no.11
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    • pp.578-583
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    • 2018
  • Telomeres are specialized nucleoprotein complexes that function to protect eukaryotic chromosomes from recombination and erosion. Several telomere binding proteins (TBPs) have been characterized in higher plants, but their detailed in vivo functions at the plant level are largely unknown. In this study, we identified and characterized OsTRFL1 (Oryza sativa Telomere Repeat-binding Factor Like 1) in rice, a monocot model crop. Although OsTRFL1 did not directly bind to telomere repeats $(TTTAGGG){_4}$ in vitro, it was associated with telomeric sequences in planta. OsTRFL1 interacted with rice TBPs, such as OsTRBF1 and RTBP1, in yeast and plant cells as well as in vitro. Thus, it seems likely that the association of OsTRFL1 with other TBPs enables OsTRFL1 to bind to telomeres indirectly. T-DNA inserted OsTRFL1 knock-out mutant rice plants displayed significantly longer telomeres (6-25 kb) than those (5-12 kb) in wild-type plants, indicating that OsTRFL1 is a negative factor for telomere lengthening. The reduced levels of OsTRFL1 caused serious developmental defects in both vegetative and reproductive organs of rice plants. These results suggest that OsTRFL1 is an essential factor for the proper maintenance of telomeres and normal development of rice.

Black Rice (Oryza sativa L. var. japonica) Hydrolyzed Peptides Induce Expression of Hyaluronan Synthase 2 Gene in HaCaT Keratinocytes

  • Sim, Gwan-Sub;Lee, Dong-Hwan;Kim, Jin-Hwa;An, Sung-Kwan;Choe, Tae-Boo;Kwon, Tae-Jong;Pyo, Hyeong-Bae;Lee, Bum-Chun
    • Journal of Microbiology and Biotechnology
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    • v.17 no.2
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    • pp.271-279
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    • 2007
  • Black rice (Oryza sativa L. var. japonica) has been used in folk medicine in Asia. To understand the effects of black rice hydrolyzed peptides (BRP) from germinated black rice, we assessed the expression levels of about 20,000 transcripts in BRP-treated HaCaT keratinocytes using human 1A oligo microarray analysis. As a result, the BRP treatment showed a differential expression ratio of more than 2-fold: 745 were activated and 1,011 were repressed. One of the most interesting findings was a 2-fold increase in hyaluronan synthase 2 (HAS2) gene expression by BRP. Semiquantitative RT-PCR showed that BRP increased HAS2 mRNA in dose-dependent manners. ELISA showed that BRP effectively increased hyaluronan (HA) production in HaCaT keratinocytes.

Phytosterols from the Rice (Oryza sativa) Bran

  • Jung, Ye-Jin;Park, Ji-Hae;Shrestha, Sabina;Song, Myoung-Chong;Cho, Suengmok;Lee, Chang-Ho;Han, Daeseok;Baek, Nam-In
    • Journal of Applied Biological Chemistry
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    • v.57 no.2
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    • pp.175-178
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    • 2014
  • Three phytosterols of rare occurrence, schleicheol 2 (1), $7{\beta}$-hydroxysitosterol (2), and $7{\alpha}$-hydroxysitosterol (3), were isolated from the n-hexane fraction of rice (Oryza sativa) bran, for the first time. Some nuclear magnetic resonance (NMR) assignments in the literatures are inaccurate. This study employed two-dimensional NMR experiments to identify exact peak assignments.

Development of rice(Oryza sativa L.) transformation system to improve callus utilization (캘러스 활용도를 향상시키기 위한 벼(Oryza sativa L.) 형질전환 시스템 구축)

  • Park, Ji-Sun;Moon, Ki-Beom;Ha, Jang-Ho;Jang, Ji-Young;Kim, Mi-Jin;Jeon, Jae-Heung;Park, Sang-Un;Kim, Hyun-Soon
    • Korean Journal of Breeding Science
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    • v.49 no.3
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    • pp.170-179
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    • 2017
  • Plant molecular farming has attracted a lot of attention lately in the field of mass production of industrially valuable materials by extending application of the plant as a kind of factory concept. Among them, protein expression system using rice(Oryza sativa L.) callus is a technology capable of mass culture and industrialization because of a high expression rate of a target protein. This study was carried out to develop an Agrobacterium-mediated transformation system to increase the utilization of rice callus. The transformation efficiency was improved by using the hand when seeds were de-husked for callus induction. Furthermore, we were possible induction of callus from 6 years old seed smoothly. Selection of the callus contained the target gene was required a cultivation period of at least 3 weeks, and the most efficient selection period was after 6 weeks of culture including one passage. This selection was confirmed that the gene was stably inserted into the genomic DNA of the plant cell by the southern blot analysis and progeny test. Such an efficient selection system of rice callus that can be cultured in the long term will be contribute to the industrialization of useful recombinant proteins using rice.

RGISS: Rice (Oryza sativa L. ssp. japonica) Genome Information Service System

  • Lee, Dae-Sang;Seo, Hwa-Jung;Hahn, Jang-Ho;Kong, Eun-Bae;Park, Kie-Jung
    • Genomics & Informatics
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    • v.5 no.4
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    • pp.194-195
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    • 2007
  • We have constructed the Rice Genome Information Service System (RGISS), which is an information service system of the Oryza sativa L. ssp. japonica (rice) genome, using the released version of rice Build 3.0 pseudomolecules based on the Ensembl architecture. The nonredundant library, composed of 3,360 clones of BACs, PACs, and fosmids, was used to construct supercontigs. RGISS contains 50,717 annotated genes from GenBank, 56,161 predicted genes from FgeneSH, and information on 9,587 markers, which includes STS, SSR, and EST-based RFLP. The 20,180 ESTs sequenced by the Korea National Institute of Agricultural Biotechnology (NIAB) were aligned and mapped into 168,792 exons. By gene ontology analysis, the classified protein numbers in the rice genome were 6158, 4531, and 12,364 proteins, which were mapped to molecular function, cellular component, and biological process, respectively.

Development of Protein Biomarkers for the Authentication of Organic Rice

  • Lee, Ju-Young;Lim, Jinkyu
    • Journal of Applied Biological Chemistry
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    • v.58 no.4
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    • pp.355-361
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    • 2015
  • The rice protein profiles of Oryza sativa L (Koshihikari) grown under organic and conventional cultivation regimes were compared on 2-D gels to develop diagnostic marker proteins for organic rice. The selected proteins, differentially expressed between organic and conventional rice, were compared with the differentially expressed proteins of another organic and conventional rice pairing, produced at a different location. In the first comparison among conventional, no-chemical, and organic rice grown in the same region, Korea, 13 proteins exhibiting differential expression in organic and conventionally grown plants were selected. Eight of the 13 proteins were down-regulated, and the 5 remaining proteins were up-regulated from conventional to organic rice. The second comparison pairing from Kyungju, revealed 12 differentially expressed proteins, with 8 down-regulated and 4 up-regulated proteins. Ten of the differentially expressed proteins that overlapped between the two comparison sets could not be clustered into any functional group using a functional annotation clustering tool. Further comparisons using another set of conventional and organic rice, belonging to a different variety of Oryza sativa L and produced in Sanchung, revealed 8 differentially expressed proteins, 5 of which were down-regulated and 3 of which were upregulated in the organic rice. Overall, 3 differentially expressed proteins were commonly found in all three organic rice crops. These 3 proteins, along with other overlapping differentially expressed proteins, can provide a good starting point for the development of signature proteins that can be used for the authentication of organic rice with a follow-up studies with more comparison sets.