• Title/Summary/Keyword: residue analysis

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Understanding Ancient Human Subsistence through the Application of Organic Residue Analysis on Prehistoric Pottery Vessels from the Korean Peninsula

  • Kwak, Seungki;Kim, Gyeongtaek
    • Journal of Conservation Science
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    • v.36 no.4
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    • pp.244-254
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    • 2020
  • This study investigates prehistoric human subsistence and pottery use on the Korean peninsula through the organic residue analysis of archaeological potsherds. Ancient human subsistence is one of the core topics in Korean archaeology. However, due to the high acidity of sediments, which prevents the long-term preservation of organic remains, archaeologists have been short of critical information on how these early prehistoric dwellers lived. Ceramic vessels can contain well-preserved lipids originating from past culinary practices. For a better understanding of human subsistence on the prehistoric Korean peninsula, food-processing behaviors were reconstructed by analyzing ancient lipids extracted from a pottery matrix. The potsherd samples used in the analysis in this paper were collected from major prehistoric habitation sites. The results show that subsistence strategies differed according to both location and time period and reveal how organic residue analysis can contribute to a better understanding of prehistoric human subsistence strategies.

Development of Analytical Reference Material for Proficiency Test of Pesticide Multi-residue Analysis in Tomato (토마토 농약다성분분석 정도관리용 분석표준물질 개발)

  • Kim, Jong-Hwan;Oh, Young-Gon;Choi, Sung-Gil;Hong, Su-Myeong;Kim, Sun-bae;Woo, In-Duk;Kim, Jun-Young;Seo, Jong-Su
    • Korean Journal of Environmental Agriculture
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    • v.35 no.3
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    • pp.223-233
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    • 2016
  • BACKGROUND: This study was to develop a analytical reference material including twenty pesticides in tomato. To use it for proficiency test, the suitability of homogeneity, storage stability, assigned values and uncertainty for analytical reference material were important.METHODS AND RESULTS: In order to develop a analytical reference material of tomato for multi-residue analysis of pesticides, twenty-pesticides were treated in tomato and the samples were frozen and homogenized. The homogeneity, stability, assigned value and uncertainty were calculated according to the requirements of the KS A ISO Guide 35, KS Q ISO 13528 and EURL-PT protocol. The values of the within-bottle standard variation(swb) and the between-bottle standard variation(sbb) were 0.9~6.5% of assigned value and the uncertainty(u*bb) due to inhomogeneity was also calculated as 0.6~1.9% for all pesticides. This indicated that it was satisfactory to be used as a analytical reference material. The storage stabilities of twenty-pesticides at room temperature and freezing conditions were assessed according to the requirement of the KS Q ISO Guide 35. All pesticides were stable at room temperature (20~30℃) for 8 days and freezing (-20℃) for 23 days.CONCLUSION: The feasibility of analytical reference material for pesticide multi-residue analysis in a tomato matrix was investigated. Homogeneity of within/between-bottle, uncertainty and stabilities at room temperature and freezing condition were satisfactory for a use of proficiency test and quality control. From these results, a analytical reference material would be applicable to monitor the proficiency test of pesticide analysis organizations to improve the reliability and consistency.

Simultaneous detection method for pesticide residues in meat by gas chromatograph-mass selective detector (Gas chromatograph-mass selective detector를 이용한 식육 중 잔류농약의 동시분석)

  • Hong In-Suk;Choi Yoon-Hwa;Kweon Taek-Boo;Lee Jung-Hark
    • Korean Journal of Veterinary Service
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    • v.28 no.3
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    • pp.285-294
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    • 2005
  • This study was conducted to investigate the extraction method for the determination of organochlorine, organophosphorus and synthetic pyrethroid pesticide residues in beef fat by gas chromatography-mass selective detector(GC-MSD). Twenty one pesticide residues in fat were determined using a simple and rapid procedure based on solid- phase extraction(SPE) clean-up cartridges with octadecyl $(C_{18})-bonded$ porous silica, florisil, $10\%$ deactivated florisil. a tandem $C_{18}$ and florisil and a tandem $C_{18}$ and $10\%$ deactivated florisil. Solvent-solvent extraction using acetonitrile was not satisfied to eliminate fat interference for pesticide residue analysis by GC-MSD, and the recoveries of the method in fat ranged from 16.2 to $57.3\%$ except DDT$(83.2\%)$. The recoveries of SPE methods using a tandem $C_{18}$ and Florisil was $59.6\~123.8\%$ except fenitrothion $(135.2\%)$. the SPE method was verified the satisfactory performance of pre-treatment for pesticide residues analysis in fat by GC-MSD. The efficiency of florisil deactivated with $10\%$ water has been not proved significantly on recoveries of pesticide residues in fat.

Studies on Pesticide Residues in Ostrici Radix (한국산 강활(羌活)의 잔류농약에 대한 연구)

  • Na, Seung-Young;Kim, Hong-Jun;Choi, Go-Ya;Jeong, Seung-Il;Ju, Young-Sung;Oh, Seo-Jin
    • The Korea Journal of Herbology
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    • v.21 no.4
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    • pp.37-41
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    • 2006
  • Objectives : This study has been conducted to investigate the amount of residual pesticide in herbal medicine of Osterici Radix which are purchased on Kwangju(Hanyaksarang). Methods : In order to analyze many pesticides in Osterici Radix, simultaneous multi-residue analysis of pesticides by GC-ECD/NFD was used. Results : But the amount of residual pesticide in Osterici Radix, simultaneous multi-residue analysis of pesticides by GC-ECD/NFD was used. Conclusion : These results indicate that Osterici Radix in current has safety. In future, it is considered the results of this study will be furnished the basis to succeeding studies and it is needed to extensive comparative study for the same genus-degree of relatedness.

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Development and Application of Enzyme Immunoassay for Endosulfan Residue Analysis (Endosulfan 과 그 분해산물의 Enzyme Immunoassay에 의한 분석법의 개발과 응용)

  • Suh, Yong-Tack;Shim, Jae-Han;Lee, Kang-Bong
    • Korean Journal of Environmental Agriculture
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    • v.11 no.1
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    • pp.59-66
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    • 1992
  • An enzyme immunoassay(EIA) was developed for the analysis of insecticide endosulfan and its degradation products. The sensitivity and specificity of the antibody produced were examined. Optimal conditions in the ELISA system for residue analysis were also discussed. A mixed suspension of endosulfan-hemocyanin conjugate(ES-KLH) 1.1 mg/ml and Freund's adjuvant was injected subcutaneously to white rabbits and then collected antisera were tested for titers by indirect ELISA(1/24,000). Because of difficulties in the synthesis of endosulfan peroxidase conjugate, amine derivative of endosulfan-diol was synthesized and it showed 40% of conjugate yields(2mg/ml of conjugate). the highest sensitivity obtained enzyme-conjugate was a concentration of 200ng/ml. The detection limit of endosulfan in ELISA system was 5 ppb on the standard curve. In application of ELISA for residue analysis, the recoveries were really 100% both in the spiked soil and apple sample regardless of endosulfan concentration treated. On the other hand, chlorinated hydrocarbons of similar structure with endosulfan showed low cross-reactivity$(2.2%{\sim}29.2%).$

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Use of ELISA for the Residue Analysis of Pesticides (ELISA 기법을 이용한 농약(農藥)의 잔류분석(殘留分析))

  • Lee, Kang-Bong;Suh, Yong-Tack
    • Korean Journal of Environmental Agriculture
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    • v.12 no.3
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    • pp.298-308
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    • 1993
  • Immunochemical assay, ELISA for small molecules such as pesticides are rapid, sensitive, cost effective and can easily analyze with large samples. ELISA is one of several powerful biotechnologies immediately applicable to pesticide analysis. This review lists the advantages and disadvantages of the ELISA and elucidate the steps in assay development using examples from this laboratory. The focus is primarily on hapten synthesis strategies, protein conjugation, Immunization, assay format, and assay validation.

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Probabilistic Analysis of Fatigue Behavior of Induction Hardened Steel (고주파 열처리된 SAE1055 강의 피로거동 및 이의 확률론적 평가)

  • Lee, Seon-Ho;Lee, Seung-Pyo;Kang, Ki Weon
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.37 no.3
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    • pp.429-436
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    • 2013
  • This study considers how the fatigue behavior and probabilistic properties of SAE1055 steel are related to its hardness level. SAE1055 steel was heat-treated using induction hardening. Five types of specimens were prepared (A: base material, B: through hardened material with HV390, C: through hardened material with HV510, D: through hardened material with HV700, and E: surface hardened material with HV700). Fatigue tests were performed under a stress ratio of R = -1 using a 4-point rotary bending fatigue tester. The fatigue behaviors were greatly influenced by the hardness, but the fatigue limit did not increase over a hardness of HV510. In addition, the effect of the hardness level on the failure mechanism was evaluated using a scanning electron microscope. The probabilistic properties of the fatigue life were investigated using a probabilistic S-N approach, and the effect of the hardness level on these properties was evaluated using a residue analysis.

Residue analysis of spinetoram and spinosad on paprika leaf using the modified QuEChERS pre-treatment methods

  • Kim, Young-Shin;Yang, Jun-Young;Jin, Na-Young;Yu, Yong-Man;Youn, Young-Nam;Lim, Chi-Hwan
    • Korean Journal of Agricultural Science
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    • v.44 no.4
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    • pp.487-494
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    • 2017
  • Spinosad and spinetoram are widely used insecticides for the control of lepidopteran larvae, leaf miners, and thrips; however, they might also have low toxicity toward beneficial insects like bees. Because these pesticides are easily photolyzed by ultraviolet radiation, the QuEChERS method, with its simple pretreatment procedure, is often used for analyzing residues of spinosad and spinetoram. The present study performed a residue analysis using a modified QuEChERS method by pretreating with ammonium salt. The limit of detection (LOD) of the modified method was 0.05 mg/kg and the limit of quantification (LOQ) was 0.25 mg/kg. The coefficient of determination ($R^2$) for the calibration curve was 0.999. Also, we examined any change in the adhesion of spinosad and spinetoram on the plants depending on a spray volume. The adhesion was approximately 70% when the spray volume was increased from 60 L to 120 L per 10 a whereas the adhesion was approximately 37% when the spray volume was increased from 125 L to 250 L. This showed that the amount of adhesion decreased with the higher spray volume. The efficacy result of spinetoram was that over 90% of Frankliniella occidentalis was controlled with the application volume of 125 L per 10 a. Therefore, the result of this study indicates that control of insects is effective and sufficient with a spray volume of 125 L per 10 a in paprika cultivation facilities.

Study for Residue Analysis of Fluxametamid in Agricultural Commodities

  • Kim, Ji Young;Choi, Yoon Ju;Kim, Jong Soo;Kim, Do Hoon;Do, Jung Ah;Jung, Yong Hyun;Lee, Kang Bong;Kim, Hyochin
    • Korean Journal of Environmental Agriculture
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    • v.38 no.1
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    • pp.1-9
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    • 2019
  • BACKGROUND: Accurate and simple analytical method determining Fluxametamid residue was necessary in various food matrices. Additionally, fulfilment of the international guideline of Codex (Codex Alimentarius Commission CAC/GL 40) was required for the analytical method. In this study, we developed Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) method to determine the Fluxametamid residue in foods. METHODS AND RESULTS: Fluxametamid was extracted with acetonitrile, partitioned and concentrated with dichloromethane. To remove the interferences, silica SPE cartridge was used before LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry) analysis with $C_{18}$ column. Five agricultural commodities (mandarin, potato, soybean, hulled rice, and red pepper) were used as a group representative to verify the method. The liner matrix-matched calibration curves were confirmed with coefficient of determination ($r^2$) greater than 0.99 at calibration range of 0.001-0.25 mg/kg. The limits of detection and quantification were 0.001 and 0.005 mg/kg, respectively. Mean average accuracies were shown to be 82.24-115.27%. The precision was also shown to be less than 10% for all five samples. CONCLUSION: The method investigated in this study was suitable to the Codex guideline for the residue analysis. Thus, this method can be useful for determining the residue in various food matrices as routine analysis.