• Title, Summary, Keyword: prosapogenin

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In vitro Culture of Adventitious Roots from Dioscorea nipponica Makino for the Production of Steroidal Saponins (부채마 (Dioscorea nipponica Makino)의 부정근 배양조건과 Steroidal Saponin의 기내생산)

  • An Jung-Hee;Son Kun-Ho;Sohn Ho-Yong;Kwon Soon-Tae
    • Journal of Plant Biotechnology
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    • v.32 no.3
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    • pp.217-223
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    • 2005
  • Effects of growth regulators on growth of adventitious roots and accumulation of steroidal saponins, such as dioscin, prosapogenin A and prosapogenin C, in cultures of Dioscorea nipponica were determined. The maximum growth of adventitious roots was observed in MS medium supplemented with 30 g/L sucrose and 1.0 mg/L NAA. Addition of BA in combination with NAA appeared to be no effective in the growth of adventitious roots. Among the twenty different adventitious roots formed from different seeds, strain No. 10 was selected based on production ability of dioscin, and its stability through the successive liquid culture. During the first 4 weeks of incubation, contents of steroidal saponins in adventitious roots were negligible but the contents were markedly increased at 5 weeks of incubation. Dioscin and prosapogenin C content in IBA-treated adventitious roots were significantly higher than those in NAA-treated roots. However, content of prosapogenin A was not significantly different among NAA or IBA level. Results provide that liquid culture of adventitious roots of D. nipponica have a potential for mass production of dioscin including prosapogenin A and prosapogenin C.

Study on 'Chu-Suk'(VI) -Prosapogenin in Pods of Gleditschia officinalis- (추석(秋石)에 관(關)한 연구(硏究)(VI) -조협 꼬투리의 Prosapogenin에 관하여-)

  • Lee, Eun-Ock;Yu, Chae-Seun
    • Korean Journal of Pharmacognosy
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    • v.9 no.2
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    • pp.93-97
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    • 1978
  • From the crude saponin obtained from the pods of Gleditschia officinalis seven spots were identified by TLC and quantity of gleditschia B was the highest among them. Ten kinds of prosapogenins were identified from the partial hydrolysates of crude saponin and found that prosapogenin E contain oleanolic acid as a sapogenin and prosapogenin F does echinocystic acid as the sapogenin. Hydrolysis of crude saponin yield glucose and rhamnose and the same sugar were also identified from the mixture of prosapogenin E and F.

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Changes in the Contents of Prosapogenin in the Skin White ginseng(Panax ginseng) Depending on Extracting Batches (피부백삼의 추출 조건에 따른 프로사포게닌 함량 변화)

  • Im, Byung Ok;Cho, Soon Hyun;Ko, Sung Kwon
    • Korean Journal of Pharmacognosy
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    • v.45 no.4
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    • pp.315-319
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    • 2014
  • This study compared the contents of ginseng prosapogenin depending on the extracting conditions of Skin White ginseng(Panax ginseng) to provide basic information for developing Skin White ginseng-based functional foods. Our findings show that the content of crude saponin peaked at 4 hours of extraction(SWG-4) and when extracted at $100^{\circ}C$. However, the content of total saponin reached its height at 8 hours of extraction at $100^{\circ}C$(SWG-8). On the other hand, the content of prosapogenin reached their heights at 60 hours of extraction(SWG-60), followed by 60 hours of extraction at $100^{\circ}C$. And at $100^{\circ}C$ the main prosapogenin of the content of Red and Black ginseng ginsenoside $Rg_3$, $Rg_5$ and $Rk_1$ reached their heights at 60 hours of extraction(SWG-60), followed by 60 hours of extraction.

Effect of Ginseng Saponin Fraction on Absorption of Cholesterol and Serum Lipid Components (인삼 사포닌 분획이 Cholesterol의 흡수 및 혈청지질 성분에 미치는 영향)

  • 정인실;조영동
    • Journal of Ginseng Research
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    • v.9 no.2
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    • pp.232-239
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    • 1985
  • The effects of total saponin, triol and diol saponin, and prosapogenin of ginseng on the absorption of cholesterol and the component of serum lipids was observed as a function of time using isotopic method. The following results were obtained. 1) Labelled cholesterol absorption was observed to be enhanced after adminstrations of total saponin, triol saponin, and. prosapogenin of ginseng to rat (Sprague Dawley). 2) Decreases in the amount of serum lipid were observed after administration of total saponin and prosapogenin of ginseng.

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Changes in the contents of prosapogenin in Red ginseng (Panax ginseng) depending on the extracting conditions

  • Kim, Shin Jung;Shin, Jin Young;Ko, Sung Kwon
    • Journal of Ginseng Research
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    • v.40 no.1
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    • pp.86-89
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    • 2016
  • This study compared the contents of prosapogenin depending on the extracting conditions of Red ginseng to provide basic information for developing Red ginseng-based functional foods. The content of ginsenoside Rg3 reached their maximum value at 24 h of extraction, followed by 36 h and 72 h of extraction at $100^{\circ}C$.

The Comparison of Ginseng Prosapogenin Composition and Contents in Red and Black Ginseng (흑삼과 홍삼의 인삼 프로사포게닌 성분 비교)

  • Jo, Hee-Kyung;Sung, Min-Chang;Ko, Sung-Kwon
    • Korean Journal of Pharmacognosy
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    • v.42 no.4
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    • pp.361-365
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    • 2011
  • The objective of this study is to provide basic information for developing a high-value ginseng product using ginseng saponin and prosapogenin. In order to achieve the proposed objective ginsenoside compositions of Black (BG) and Red (RG) ginseng extract with 95% ethyl alcohol were examined by means of HPLC. The crude saponin and ginsenoside composition of processed ginseng products were analyzed and compared, with BG topping the list with a crude saponin content of 7.53%, followed by RG (5.29%). Ginseng prosapogenin (ginsenosides $Rg_2$, $Rg_3$, $Rg_5$, $Rg_6$, $Rh_1$, $Rh_4$, $Rk_1$, $Rk_3$, $F_1$ and $F_4$) in BG was found to be contained almost 2.6 times as much as that in RG. Ginsenosides $Rg_3$, $Rg_5$, $Rk_1$, $Rh_4$ and $F_4$ in BG in particular were found to be almost 3 times as much as those in RG. $Rg_6$ and $Rk_3$ in BG were also found to be almost 4 times as much as those in RG.

Analysis of Ginsenosides of Black Ginseng (흑삼의 인삼 사포닌 분석)

  • Han Sung Tai;Whang Wan Kyun;Kim Il Hyuk;Yang Byung Wook;Cho Soon Hyun;Ko Sung Kwon
    • YAKHAK HOEJI
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    • v.49 no.6
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    • pp.490-494
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    • 2005
  • The objective of this study is to provide the basic information for developing a high-value ginseng product using ginseng saponin and prosapogenin. In order to achieve such aim, Ginsenoside compositions of black ginseng (BG) extracts with various solvent conditions were examined by HPLC. The total saponin and the prosapogenin content of 95$\%$ ethyl alcohol extract were higher than that of the either 50$\%$ ethyl alcohol extract or distilled water extract. As a result, the order of the total saponin and the prosapogenin content was 1) 95$\%$ ethyl alcohol,2) 50$\%$ ethyl alcohol,3) the first and second mixture of 95$\%$ ethyl alcohol, distilled water, and 4) distilled water extract. In the case of fine black ginseng (FBG), the first and second mixture extracts of 95$\%$ ethyl alcohol and distilled water were the highest. In addition, the ratio of the protopanaxadiol group and the protopanaxatriol group (PD/PT) showed that the ratio of BG ranged from 0.304 to 0.601, while the ratio of FBG ranged from 1.166 to 1.657.

The transformation of ginsenosides by acid catalysis in gastric pH

  • Han, Byung-Hoon;Park, Myung-Hwan;Han, Yong-Nam;Woo, Lin-Keun;Ushio-Sankawa;Shoji-Yahara;Osamu-Tanaka
    • Archives of Pharmacal Research
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    • v.4 no.1
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    • pp.25-31
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    • 1981
  • The ginsenosides of Korean ginseng decomposed profoundly to produce artifact products of prosapogenin $A_{1}$, $A_{2}$ and $A_{3}$ from ginsenoside Rg$_{1}$, prosapogenin $C_{1}$, $C_{2}$ and $C_{3}$ from ginsenoside Re, and prosapogenin E$_{1}$, E$_{2}$ and E$_{3}$ from ginsenoside Rb$_{1}$ by the acid treatment under physiological condition such as 37.deg.C incubation in 0.1 N HCI. 2. The chemical structures of the artifact substances were determined by the analysis CMR and mass spectra of TMS derivatives as following; table omitted.

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Steroidal Saponins from the Rhizomes of Smilax china

  • Kim, Sung-Whan;Chung, Kyu-Charn;Son, Kun-Ho;Kang, Sam-Sik
    • Korean Journal of Pharmacognosy
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    • v.20 no.2
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    • pp.76-82
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    • 1989
  • Five known diosgenin glycosides have been isolated from the MeOH extract of Smilax china rhizomes and characterized as prosapogenin A of dioscin, dioscin, gracillin, methyl protogracillin, methyl protodioscin and its corresponding 22-hydroxy analog. This is the first isolation of the former four compounds from this plant. ${\beta}-Sitosterol\;glucoside$ was also isolated and identified.

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Further Spirostanol Glycosides from the Tuber of Liriope spicata

  • Do, Jae-Chul;Sung, Yong-Kyung;Son, Kun-Ho
    • Korean Journal of Pharmacognosy
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    • v.22 no.2
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    • pp.73-77
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    • 1991
  • Further studies have been done on the constituents of the tubers of Liriope spicata $L_{OUR}$ (Liliaceae). Four steroidal glycosides, tentatively designated as compounds I. II, III and IV, were isolated from the n-BuOH soluble fraction of this plant. The structures of these glycosides were established as ${\beta}-sitosterol$ glucoside, prosapogenin II of spicatoside A, ophiopogonin B, and prosapogenin III of spicatoside A.

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