• 제목, 요약, 키워드: prevention

검색결과 22,776건 처리시간 0.077초

Cytosolic prion protein induces apoptosis in human neuronal cell SH-SY5Y via mitochondrial disruption pathway

  • Wang, Xin;Dong, Chen-Fang;Shi, Qi;Shi, Song;Wang, Gui-Rong;Lei, Yan-Jun;Xu, Kun;An, Run;Chen, Jian-Ming;Jiang, Hui-Ying;Tian, Chan;Gao, Chen;Zhao, Yu-Jun;Han, Jun;Dong, Xiao-Ping
    • BMB Reports
    • /
    • v.42 no.7
    • /
    • pp.444-449
    • /
    • 2009
  • Different neurodegenerative disorders like prion disease, is caused by protein misfolding conformers. Reverse-transfected cytosolic prion protein (PrP) and PrP expressed in the cytosol have been shown to be neurotoxic. To investigate the possible mechanism of neurotoxicity due to accumulation of PrP in cytosol, a PrP mutant lacking the signal and GPI (CytoPrP) was introduced into the SH-SY5Y cell. MTT and trypan blue assays indicated that the viability of cells expressing CytoPrP was remarkably reduced after treatment of MG-132. Obvious apoptosis phenomena were detected in the cells accumulated with CytoPrP, including loss of mitochondrial transmembrane potential, increase of caspase-3 activity, more annexin V/PI-double positive-stained cells and reduced Bcl-2 level. Moreover, DNA fragmentation and TUNEL assays also revealed clear evidences of late apoptosis in the cells accumulated CytoPrP. These data suggest that the accumulation of CytoPrP in cytoplasm may trigger cell apoptosis, in which mitochondrial relative apoptosis pathway seems to play critical role.

Laboratory Confirmation of A Suspicious Meningococcal Meningitis Death Case

  • Zhang Tie-Gang;He Xiong;Chen Li-Juan;He Jing-Guo;Luo Ming;Yang Jie;Shao Zhu-Jun;Sun Mei-Ping
    • Journal of Microbiology
    • /
    • v.44 no.4
    • /
    • pp.457-460
    • /
    • 2006
  • A suspicious meningococcal meningitis death case was reported to the Beijing CDC. The blood specimen was analyzed via multi-PCR and MLST. 6 isolates from close contacts were analyzed via PFGE and MLST. According to the results of the above analyses, the cause of this case was identified as a serogroup A Neisseria meningitidis, which, in terms of sequence typing, belonged the ST7 group.

병원간호사의 낙상예방간호 수행 영향요인 (Factors influencing fall prevention nursing performance of hospital nurses)

  • 장경숙;김혜숙
    • 한국응급구조학회지
    • /
    • v.20 no.3
    • /
    • pp.69-83
    • /
    • 2016
  • Purpose: The purpose of this study was to explore the factors influencing evidence-based fall prevention nursing performance of hospital nurses. Methods: A self-reported questionnaire was completed by 344 nurses from three general hospitals from January 20 to March 10, 2013. The study instruments included general characteristics of the subjects, and awareness and performance of fall prevention. Data were analyzed by t test, ANOVA, Pearson's correlation, and multiple regression using SPSS v. 20.0. Results: There were statistically significant differences in awareness and performance according to age, marital status, clinical experiences, workplace, experience of fall prevention education, knowledge of fall prevention, compliance with fall prevention, attention level toward prevention, recognition level of potential falls, nurse responsibility for falls, importance of fall prevention, efforts level for fall prevention, and awareness score of falls prevention. There was a positive correlation among awareness and performance of fall prevention. Based on the multiple regression analysis, compliance with fall prevention, efforts level for fall prevention, and awareness score of falls prevention were significant predictors for performance of fall prevention. The explanation power of the model was 64.1%. Conclusion: The findings revealed the need to develop an effective nursing intervention to improve hospital nurses' performance for fall prevention.

Decision-Making Model Research for the Calculation of the National Disaster Management System's Standard Disaster Prevention Workforce Quota : Based on Local Authorities

  • Lee, Sung-Su;Lee, Young-Jai
    • Journal of Information Technology Applications and Management
    • /
    • v.17 no.3
    • /
    • pp.163-189
    • /
    • 2010
  • The purpose of this research is to develop a decision-making model for the calculation of the National Disaster Management System's standard prevention workforce quota. The final purpose of such model is to support in arranging a rationally sized prevention workforce for local authorities by providing information about its calculation in order to support an effective and efficient disaster management administration. In other words, it is to establish and develop a model that calculates the standard disaster prevention workforce quota for basic local governments in order to arrange realistically required prevention workforce. In calculating Korea's prevention workforce, it was found that the prevention investment expenses, number of prevention facilities, frequency of flood damage, number of disaster victims, prevention density, and national disaster recovery costs have positive influence on the dependent variable when the standard prevention workforce was set as the dependent variable. The model based on the regression analysis-which consists of dependent and independent variables-was classified into inland mountainous region, East coast region, Southwest coastal plain region to reflect regional characteristics for the calculation of the prevention workforce. We anticipate that the decision-making model for the standard prevention workforce quota will aid in arranging an objective and essential prevention workforce for Korea's basic local authorities.

  • PDF

Colorectal Cancer Concealment Predicts a Poor Survival: A Retrospective Study

  • Li, Xiao-Pan;Xie, Zhen-Yu;Fu, Yi-Fei;Yang, Chen;Hao, Li-Peng;Yang, Li-Ming;Zhang, Mei-Yu;Li, Xiao-Li;Feng, Li-Li;Yan, Bei;Sun, Qiao
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.14 no.7
    • /
    • pp.4157-4160
    • /
    • 2013
  • Objectives: Understanding the situation of cancer awareness which doctors give to patients might lead to prognostic prediction in cases of of colorectal cancer (CRC). Methods: Subsets of 10,779 CRC patients were used to screen the risk factors from the Cancer Registry in Pudong New Area in cancer awareness, age, TNM stage, and gender. Survival of the patients was calculated by the Kaplan-Meier method and assessed by Cox regression analysis. The views of cancer awareness in doctors and patients were surveyed by telephone or household. Results: After a median observation time of 1,616 days (ranging from 0 to 4,083 days) of 10,779 available patients, 2,596 of the 4,561 patients with cancer awareness survived, whereas 2,258 of the 5,469 patients without cancer awareness and 406 of the 749 patients without information on cancer awareness died of the disease. All-cause and cancer-specific survival were poorer for the patients without cancer awareness than those with (P < 0.001 for each, log-rank test). Cox multivariate regression analysis showed that cancer concealment cases had significantly lower cancer-specific survival (hazard ratio (HR) = 1.299; 95 % confidence interval (CI): 1.200-1.407)and all-cause survival (HR = 1.324; 95 % CI: 1.227-1.428). Furthermore, attitudes of cancer awareness between doctors and patients were significantly different (P < 0.001). Conclusion: Cancer concealment, not only late-stage tumor and age, is associated with a poor survival of CRC patients.

The Molecular Characterization of Serogroup C Neisseria meningitidis Strains Circulating in Beijing

  • Zhang, Tie-Gang;He, Jing-Guo;He, Xiong;Chen, Li-Juan;Shao, Zhu-Jun;Sun, Mei-Ping
    • Journal of Microbiology
    • /
    • v.44 no.6
    • /
    • pp.685-688
    • /
    • 2006
  • The aim of this study was to characterize the molecular features of serogroup C Neisseria meningitidis strains circulating in Beijing, China. Twenty out of 23 strains belonged to ST 4821. The causative serosubtype for meningococcal meningitis was P1.12-1,16-8. All of the strains expressed class 3 PorB protein. Among the five pulsed-field gel electrophoresis patterns observed, pattern III predominated.

대학생의 신체 장애인에 대한 인식과 태도 비교 연구 (Comparative Study on Attitudes toward the Disabled person of University Students)

  • 김미희
    • 한국응급구조학회지
    • /
    • v.10 no.2
    • /
    • pp.5-14
    • /
    • 2006
  • The purpose of this study was supply basic information and positive attitude needed to understand the disabled person through the analysing EMT Dept. student reaction on the disabled person. The subjects for this study were 251 students from G-college in In-Cheon City. The subjects were separated as the EMT dept. students and general dept student. The instrument used for this study were Lazer. Bonett and Granaff(1973). The scale of attitude on the disabled person. Data were analyzed by use of two-sample t-test and correlation coefficient with SAS program. Results are as follows ; 1. Frist, the EMT Dept. students showed more positive and optimistic attitude on the disabled person as safety prevention with their family life and safety prevention with first impression, but the general dept. student showed more positive and optimistic attitude on the disabled person as safety prevention with their self-consciousness, safety prevention with operative capacity, safety prevention with social life and safety prevention with school life. 2. Second, on attitude of 1 or 2 grade, first grade showed more positive and optimistic attitude on the disabled person as safety prevention with self-consciousness and safety prevention with operative capacity. Second grade showed more positive and optimistic attitude on the disabled person as safety prevention with social life, safety prevention with family life and safety prevention with school life. and first impression. But disabled person as safety prevention with self-consciousness, safety prevention with operative capacity, safety prevention with social life, safety prevention with school life and first impression. 3. Third, in interrelation effect analysing each section department and grade. They showed and interrelated more positive and optimistic attitude on the disabled person as safety prevention with their social life and first impression. As a results, it is important to understand disabled person whose physical condition. Especially, this study suggests to need to endeavor safety prevention for disabled person.

  • PDF

Removal of the Glycosylation of Prion Protein Provokes Apoptosis in SF126

  • Chen, Lan;Yang, Yang;Han, Jun;Zhang, Bao-Yun;Zhao, Lin;Nie, Kai;Wang, Xiao-Fan;Li, Feng;Gao, Chen;Dong, Xiao-Ping;Xu, Cai-Min
    • BMB Reports
    • /
    • v.40 no.5
    • /
    • pp.662-669
    • /
    • 2007
  • Although the function of cellular prion protein (PrP$^C$) and the pathogenesis of prion diseases have been widely described, the mechanisms are not fully clarified. In this study, increases of the portion of non-glycosylated prion protein deposited in the hamster brains infected with scrapie strain 263K were described. To elucidate the pathological role of glycosylation profile of PrP, wild type human PrP (HuPrP) and two genetic engineering generated non-glycosylated PrP mutants (N181Q/N197Q and T183A/T199A) were transiently expressed in human astrocytoma cell line SF126. The results revealed that expressions of non-glycosylated PrP induced significantly more apoptosis cells than that of wild type PrP. It illustrated that Bcl-2 proteins might be involved in the apoptosis pathway of non-glycosylated PrPs. Our data highlights that removal of glycosylation of prion protein provokes cells apoptosis.

Internal Motivation, Perceived Health Competency, and Health Literacy in Primary and Secondary Cancer Prevention

  • Jung, Su Mi;Jo, Heui Sug;Oh, Hyung Won
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.17 no.12
    • /
    • pp.5127-5132
    • /
    • 2016
  • Objective: The aim of this study was to identify associations of internal motivation, perceived health competency, and health literacy with primary and secondary cancer prevention. Methods: A telephone survey was conducted with a sample of 2,700, 30-69 year olds, proportionally extracted from Gangwon Province, South Korea. The dependent variables were actions in primary and secondary prevention and the explanatory variables were 13 questions in three areas: internal motivation (4 items), perceived health competency (4 items), and health literacy (5 items). Result: Multiple linear regression analysis showed that internal motivation, perceived health competency, and health literacy positively impacted primary prevention after controlling for gender and age. As internal motivation, perceived health competency, and perceived literacy increased by 1 point, primary prevention scores increased by 0.11, 0.11, and 0.07 points, respectively. In addition, logistic regression results for secondary prevention showed that health literacy had a positive impact on secondary behavior. As health literacy increased by 1 point, the odds ratio of the practice of secondary prevention was 1.4 times higher. Conclusion: This study suggests that primary and secondary prevention of cancer are significantly related to intrinsic motivation factors, perceived health competency, and actual health literacy. Health literacy concepts that cover the capacity of health management in comprehensive areas need to be applied to education and promotion for improvement of primary and secondary prevention of cancer.