• Title, Summary, Keyword: praziquantel

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Correlation between Pharmacokinetics of Praziquantel and Extermination of Microcotyle sebastis (Monogenea) in Cultured Rockfish Sebastes schlegeli

  • Kim Chun Soo;Kim Ki Hong
    • Fisheries and aquatic sciences
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    • v.4 no.4
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    • pp.197-200
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    • 2001
  • To investigate the re-treatment time of Microcotyle sebastis by oral administration of praziquantel, the residue levels of praziquantel in plasma of rockfish Sebastes schlegeli administered orally at a dose of 200 mg/kg B.W. were analyzed by reversed-phase HPLC, and the concentrations of praziquantel in the plasma were correlated with the extermination of M. sebastis. The absorption and depletion of praziquantel in the blood of rockfish were fast and the residual concentrations of praziquantel declined below $4\mu g/mL$ within 24 hr post treatment. Most of worms were exterminated within 3 hr after oral administration of praziquantel, however, a small number of M. sebastis were not killed by the treatment until end of the experiment. Considering fast drop of praziquantel in blood and extermination pattern of M. sebastis in the present results, retreatment at an interval of 9-12 hr would be effective for eradication of M. sebastis.

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A Paragonimiasis Patient with Allergic Reaction to Praziquantel and Resistance to Triclabendazole: Successful Treatment after Desensitization to Praziquantel

  • Kyung, Sun-Young;Cho, Yong-Kyun;Kim, Yu-Jin;Park, Jeong-Woong;Jeong, Sung-Hwan;Lee, Jae-Ik;Sung, Yon-Mi;Lee, Sang-Pyo
    • The Korean Journal of Parasitology
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    • v.49 no.1
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    • pp.73-77
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    • 2011
  • Paragonimiasis is an infectious disease caused by trematodes of the genus Paragonimus. This trematode can be treated successfully with praziquantel in more than 90% of the cases. Although praziquantel is generally well tolerated, anaphylactic reactions to this drug have been reported in a few cases. We report here a 46-year-old Korean female with paragonimiasis, presumed to be due to Paragonimus westermani, who displayed an allergic reaction to praziquantel and resistance to triclabendazole treatment. The patient was successfully treated with praziquantel following a rapid desensitization procedure. Desensitization to praziquantel could be considered when no alternative drugs are available.

Treatment of Microcotyle sebastis (Monogenea: Polyopisthocotylea) Infestation with Praziquantel under Commercial Rockfish (Sebastes schlegeli) Culture Conditions

  • Kim Chun Soo;Cho Jae Bum;Ahn Kyoung Jin;Lee Jae Il;Kim Ki Hong
    • Fisheries and aquatic sciences
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    • v.5 no.2
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    • pp.141-143
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    • 2002
  • The treatment efficacy of oral administration of praziquantel against the blood-sucking monogenean Microcotyle sebastis under the commercial rockfish-culture conditions was evaluated. The quantity of praziquantel added to the food was calculated so that the amount of praziquantel ingested daily by fish was 200mg or 400mg/kg body weight (bw). The fish were fed a moist-pelleted fish meal supplemented with praziquantel at an rate of 0 (control), 200 or 400 mg of praziquantel per kg bw, respectively, for 3 times at an interval of 24 h. On 1 day to 8 days after the treatment, 10 fish were taken randomly from each net-pen daily (80 fish in each group in total), and the efficacy of each treatment was confirmed. Abundance of worms were significantly reduced in groups fed praziquantel-supplemented diets. The results clearly demonstrated that feeding the praziquantel-supplemented diet was effective for controlling M. sebastis infestation in practical commercial rockfish-culture systems without imposing any handling stress.

Light microscopic observations on the in vitro effects of praziquantel on Heterophyopsis continua (Heterophyopsis continua에 대한 praziquantel의 시험관내 효과의 광학현미경적 관찰)

  • Woo, Ho-choon;Suh, Myung-deuk;Hong, Sung-jong
    • Korean Journal of Veterinary Research
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    • v.30 no.4
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    • pp.479-485
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    • 1990
  • This study was carried out to observe in vitro effect of praziquantel on the viability and internal organ changes of Heterophyopsis continua with light microscopy. Metacercariae were collected from the perch, Lateolabrax japonicus, by artificial digestion technique and fed to 2-week old chickens. Adult worms were recovered from the small intestines of chickens 8 days after infection. For working solutions, praziquantel was diluted with TC199 medium at the concentration of 0.01, 0.1, 1 and $10{\mu}g/ml$. To each petri dish containing 10ml of solution, 5~10 worms were introduced and incubated at $37^{\circ}C$. Motlity of worms was observed at 5, 15, 30, 60 minutes, 1, 2, 4 and 6 hours after incubation. For light microscopy, worms were fixed in 10% formalin under cover glass pressure and stained with Semichon's acetocarmine. The results were as follows: 1. In $0.01{\mu}g/ml$ praziquantel, the worms had their mobility until 6 hours post treatment. However, worms in over $0.1{\mu}g/ml$ of praziquantel contracted within 5 minutes and immobilized. 2. Intestine of the worm incubated in $0.001{\mu}g/ml$ praziquantel for 5 minutes was dilated and intestinal wall was thickened. 3. In incubated over $0.1{\mu}g/ml$ praziquantel, pharynx of the worm protruded out from oral sucker. 4. The lowest effective lethal concentration of praziquantel on H. continua was $0.1{\mu}g/ml$. The worms exposed to the drug were observed to be immobilized immediately after incubation in solutions of over $0.1{\mu}g/ml$ concentration. All of the worms in early period showed severe contraction and those in late period showed severe dilation.

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Pharmacokinetics of Praziquantel in Rabbits (가토에서의 Praziquantel의 체내 동태)

  • Lee, Mi-Jeong;Shim, Chang-Koo;Lee, Min-Hwa;Kim, Shin-Keun
    • YAKHAK HOEJI
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    • v.34 no.1
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    • pp.40-46
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    • 1990
  • Pharmacokinetic studies on praziquantel in rabbits were performed in this paper. The pharmacologically active parent drug was separated from the pharmacologically inactive metabolites by HPLC method. The pharmacokinetic parameters of parent drug were obtained. In vitro partition to blood cells of praziquantel was measured. The mean value (n = 3) of partition to blood cells was 44% at concentrations between $1\;{\mu}g/ml$ and $40\;{\mu}g/ml$. Therefore, the relatively high partition to blood cells should be considered in further pharmacokinetic studies on praziquantel.

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Hypersensitive Reaction to Praziquantel in a Clonorchiasis Patient

  • Lee, Jung-Min;Lim, Hyun-Sul;Hong, Sung-Tae
    • The Korean Journal of Parasitology
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    • v.49 no.3
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    • pp.273-275
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    • 2011
  • Praziquantel is the drug of choice for clonorchiasis. Since clonorchiasis is endemic in most river basins, praziquantel has been widely used for 30 years in Korea. A 54-year-old Korean woman suffered from hypersensitive reactions, such as nausea, dyspnea, rash, and urticaria after taking the first dose of praziquantel to treat clonorchiasis. She ingested one dose again and the same symptoms appeared, and she was treated at a clinic with anti-histamines. She tried one more dose with anti-histamines but found the same symptoms. Later, she was found to pass eggs of Clonorchis sinensis and medicated with flubendazole. The hypersensitive reaction to praziquantel is rare but occurs. This is the 5th case report in the world.

Synthesis of Praziquantel Derivatives and Their In Vitro Activity Against Adult Clonorchis sinensis

  • Kim, Choong-Sup;Min, Duk-Young
    • Archives of Pharmacal Research
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    • v.21 no.6
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    • pp.744-748
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    • 1998
  • Several praziquantel derivatives have been prepared by the acylation of compound 5, and examined on their biological activity in vitro agninst adult clonorchis sinensis collected from rabbits infected with metacercariae which was isolated from Pseudorasbora parva, a second intermediate host, captured in Nakdong river in Korea.

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Control of clonorchiasis by repeated treatments with praziquantel

  • Hong, Sung-Tae;Rim, Han-Jong;Min, Duk-Young;Li, Xueming;Xu, Jingxian;Feng, Zheng;Lee, Soon-Hyung
    • The Korean Journal of Parasitology
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    • v.39 no.4
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    • pp.285-292
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    • 2001
  • The present study aimed to evaluate control efficacy of clonorchiasis by two schemes of repeated treatment with praziquantel at two endemic villages in China. Residents of one village at Guangxi Autonomous Region were treated and examined 6-monthly and of another at Liaoning Province 12-monthly, In residents that took 25 mg/kg x3 (total 75 mg/kg) of Praziquantel every 6 months for one Year the e99 Positive rate showed a significant drop from 69.0% to 17.1%. In contrast, a group of same praziquantel medication once showed a slight marginal decrease in the egg rate from 18.9% to 12.2% after one year Of 39 subjects examined 3 times, 56.4% were cured, 7.7% persistently positive, one (2.6%) reinfected after cure or newly infected, but 25.6% were persistently negative. The present finding suggests that 6-monthly medication with 75 mg/kg of praziquantel should effectively lower the prevalence but incomplete for control of clonorchiasis in heavy endemic areas.

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In vitro dffect of praziquantel on Paragonimus westermani by light and scanning electron microscopic observation (폐흡충에 대한 Praziquantel의 시험관내 작용에 관한 광학 및 연사전자현미경적 관찰)

  • 이순형;박호진
    • The Korean Journal of Parasitology
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    • v.25 no.1
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    • pp.24-36
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    • 1987
  • The effect of praziquantel on P. westermani exposed in vitro was observed by stereomicroscope, light microscope and scanning electron microscope. Following results were found. 1. The worms incubated in $0.01{\;}{\mu}g/ml$ praziquantel were moving after 26-hour incubation. However, all of them were immobilized immediately after incubation in solutions over $0.01{\;}{\mu}g/ml$ concentration. 2. All of the exposed worms showed severe vacuolization not only in tegument but in subtegument, intestine, ovary, testis, Mehlis' gland and excretory bladder. 3. Vacuoles in tegument burst out to form craters. As incubation time went on, tegumental structure was disintegrated severely. The worms exposed to praziquantel were observed to be immobilized and be vacuolized of all tissues. Disintegration of reproductive organs suggests that praziquantel have suppressive effect on egg production when the flukes are not killed. The drug effects were found more related with incubation time than with drug concentration.

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The Effect of CYP Polymorphism on Resistance against Praziquantel in Clonorchis Sinensis-infected Patients

  • Kim, Chung-Hyeon;Choi, Min-Ho;Chae, Jong-Il;Shin, Eun-Hee;Hong, Sung-Tae
    • Molecular & Cellular Toxicology
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    • v.3 no.3
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    • pp.195-197
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    • 2007
  • Currently praziquantel is used for treatment of not only clonorchiasis but also other trematodes and cestodes. But cure rate of praziquantel is just 60-80% for most trematodes. It needs for the treatment-failed patients to have more drugs. The cause of failure of treatment is not studied. We just know that the blood level of praziquantel is severely different among the people. We guess that this factor may influence the results of treatment. In an endemic area of human clonorchiasis in Heilongjiang Providence, China, 78 subjects were selected for the study. Three doses of 25 mg/kg (total 75 mg/kg) of praziquantel were administered to 78 clonorchiasis patients. After three weeks of treatment, stool examination was undertaken once again to confirm the cured and uncured subjects. To analyze SNP (single nucleotide polymorphism) of CYP3A5 PS2-1, CYP3A5 PS2-2, and CYP3A5*6, PCR method was done with specifically designed primers. The mutation rates of all sites were not significant statistically. The number of subjects was too small, so we need more subjects and other delivery proteins of bile ducts (ex. MRP etc.) were also considered for effects of praziquantel. We analyzed, for the first time, the entire CYP3A5 gene in a French population, using a polymerase chain reaction- single strand conformational polymorphism (PCR-SSCP) strategy.