• Title, Summary, Keyword: miR-181a-5p

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Hsa-miR-181a-5p Expression and Effects on Cell Proliferation in Gastric Cancer

  • Chen, Gang;Shen, Zhi-Li;Wang, Ling;Lv, Chun-Ye;Huang, Xin-En;Zhou, Rong-Ping
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.6
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    • pp.3871-3875
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    • 2013
  • Purpose: MicroRNAs (miRNAs) are small endogenous, non-coding, single-stranded RNAs (approximately 22 nt). Accumulating evidence has shown that aberrant miRNA expression is pronounced and correlated with gastric cancer genesis and progression. Materials and Methods: Expression levels of miR-181a-5p in GC tissues and cell lines were assessed by qRT-PCR and tested for correlation with clinical features. In addition, effects of miR-181a-5p on GC cell growth were investigated. Results: Our findings indicate that miR-181a-5p is upregulated in GC, in correlation with lymph node invasion, nerve invasion and vascular invasion (P<0.05). Enforced expression of miR-181a -5p promoted cell proliferation ability. Conclusions: This study suggested that increased miR-181a-5p is related to GC progression. MiR-181a-5p may represent a potential therapeutic target for GC.

Upregulation of MicroRNA 181c Expression in Gastric Cancer Tissues and Plasma

  • Cui, Mei-Hua;Hou, Xiao-Lin;Lei, Xiao-Yan;Mu, Fang-Hong;Yang, Gui-Bin;Yue, Lin;Fu, Yi;Yi, Guo-Xing
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.5
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    • pp.3063-3066
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    • 2013
  • Objective: To test the microRNA-181c (miR-181c) expression in tissues and plasma of gastric cancer (GC) cases, analyze any correlations, and explore the possibility of miR-181c as a potential molecular marker for GC diagnosis. Materials and Methods: Relative miR-181c expression levels in cancers and plasma from 30 GC patients was tested using reverse transcription-real-time fluorescent quantitation PCR and compared to that in samples from 30 gastric ulcer and 30 chronic gastritis patients. Results: The miR-181c expression level in the GC tissues was significantly higher than that in the gastric ulcer and chronic gastritis tissues (P = 0.000), as was the miR-181c expression level in the GC plasma (P = 0.000). We determined that miR-181c expression in GC plasma was positively correlated to its expression in the GC tissues (P = 0.000). Conclusions: The expression of miR-181c is upregulated in GC tissues and plasma, and the miR-181c expression level in GC plasma is positively correlated to that in the corresponding cancer tissues. Plasma miR-181c is possibly a new serological marker for GC diagnosis.

Exosomal miR-181b-5p Downregulation in Ascites Serves as a Potential Diagnostic Biomarker for Gastric Cancer-associated Malignant Ascites

  • Yun, Jieun;Han, Sang-Bae;Kim, Hong Jun;Go, Se-il;Lee, Won Sup;Bae, Woo Kyun;Cho, Sang-Hee;Song, Eun-Kee;Lee, Ok-Jun;Kim, Hee Kyung;Yang, Yaewon;Kwon, Jihyun;Chae, Hee Bok;Lee, Ki Hyeong;Han, Hye Sook
    • Journal of Gastric Cancer
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    • v.19 no.3
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    • pp.301-314
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    • 2019
  • Purpose: Peritoneal carcinomatosis in gastric cancer (GC) patients results in extremely poor prognosis. Malignant ascites samples are the most appropriate biological material to use to evaluate biomarkers for peritoneal carcinomatosis. This study identified exosomal MicroRNAs (miRNAs) differently expressed between benign liver cirrhosis-associated ascites (LC-ascites) and malignant gastric cancer-associated ascites (GC-ascites), and validated their role as diagnostic biomarkers for GC-ascites. Materials and Methods: Total RNA was extracted from exosomes isolated from 165 ascites samples (73 LC-ascites and 92 GC-ascites). Initially, microarrays were used to screen the expression levels of 2,006 miRNAs in the discovery cohort (n=22). Subsequently, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analyses were performed to validate the expression levels of selected exosomal miRNAs in the training (n=70) and validation (n=73) cohorts. Furthermore, carcinoembryonic antigen (CEA) levels were determined in ascites samples. Results: The miR-574-3p, miR-181b-5p, miR-4481, and miR-181d were significantly downregulated in the GC-ascites samples compared to the LC-ascites samples, and miR-181b-5p showed the best diagnostic performance for GC-ascites (area under the curve [AUC]=0.798 and 0.846 for the training and validation cohorts, respectively). The diagnostic performance of CEA for GC-ascites was improved by the combined analysis of miR-181b-5p and CEA (AUC=0.981 and 0.946 for the training and validation cohorts, respectively). Conclusions: We identified exosomal miRNAs capable of distinguishing between non-malignant and GC-ascites, showing that the combined use of miR-181b-5p and CEA could improve diagnosis.

MicroRNA Expression in Leukemia Cell Line(K562 cell) Using Rhus Verniciflua Stokes (건칠(乾漆)을 이용한 K562 만성 골수성 백혈병 세포주에서의 MicroRNA 발현 규명)

  • Choi, Hyun Sook
    • The Korea Journal of Herbology
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    • v.34 no.6
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    • pp.71-78
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    • 2019
  • Objective : The purpose of the study was to identify expression profiling of miRNAs associated with cancers after treating allergen-removed Rhus Verniciflua Stokes and allergen-removed Rhus Verniciflua Stokes fumigaed Angelica gigas on leukemia cell lines. Methods : miRNA expression has been analyzed using miRNA array method through denaturation and hybridization after isolating the total RNA from leukemic cell line treated with 100 ㎍/㎖ of aRVS and aRVS-A each. Microarray expressions were interpreted as 'significant' on miRNAs when decreased less than 0.5 fold or increased more than 1.5 fold compared with the control group. Results : Among 158 miRNAs in total, 32 miRNAs were significantly presented in miRNAs expression. miRNA has been activated with a variety of genes for predicted targets, and the overexpressed miRNAs were categorized according to proliferation and metastasis of cancer in this study. The findings were reported that seven miRNAs (let-7b, miR-193a-5p, 296-3p, 26a, 22, 124a, 92b) showed significant expressions on proliferation and growth, seven miRNAs (miR-193a-5p, 26a, 200c, 183, 124a, 198, 210) presented meaningful expressions on invasion and metastasis, two miRNAs (let-7b, miR-210) were highly expressed on angiogenesis, five miRNAs (let-7b, miR-26a, 181d, 181c, 296-5p) related with apoptosis, and six miRNAs (let-7b, miR-200c, 183, 370, 124a, 191) were associated with prognosis of cancer and early diagnostic factors for cancer. Conclusion : The mechanism of miRNA takes a role in diagnosis, treatment, and prognotic factors for cancer as well. This study suggested that further detailed research on overexpression of specific miRNA should be carried out continuously in the future.

Effects of Motor Imagery Practice in Conjunction with Repetitive Transcranial Magnetic Stimulation on Stroke Patients

  • Ji, Sang-Goo;Cha, Hyun-Gyu;Kim, Ki-Jong;Kim, Myoung-Kwon
    • Journal of Magnetics
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    • v.19 no.2
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    • pp.181-184
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    • 2014
  • The aim of the present study was to examine whether motor imagery (MI) practice in conjunction with repetitive transcranial magnetic stimulation (rTMS) applied to stroke patients could improve theirgait ability. This study was conducted with 29 subjects diagnosed with hemiparesis due to stroke.The experimental group consisted of 15 members who were performed MI practice in conjunction with repetitive transcranial magnetic stimulation, while the control group consisted of 14 members who were performed MI practice and sham therapy. Both groups received traditional physical therapy for 30 minutes a day, 5 days a week, for 6 weeks; additionally, they received mental practice for 15 minutes. The experimental group was instructed to perform rTMS and the control group was instructed to apply sham stimulation for 15 minutes. Gait analysis was performed using a three-dimensional motion capture system, which is a real-time tracking device that delivers data via infrared reflective markers using six cameras. Results showed that the velocity, step length, and cadence of both groups were significantly improved after the practice (p<0.05). Significant differences were found between the groups in velocity and cadence (p<0.05) as well as with respect to the change rate (p<0.05) after practice. The results showed that MI practice in conjunction with rTMS is more effective in improving gait ability than MI practice alone.

Expression of MicroRNA-221 in Korean Patients with Multiple Myeloma (한국인의 다발성골수종 환자에서 MicroRNA-221의 발현)

  • Choi, Woo-Soon
    • Korean Journal of Clinical Laboratory Science
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    • v.50 no.2
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    • pp.197-204
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    • 2018
  • Multiple myeloma (MM) is the leading cause of death among hematologic neoplasms. Recently, microRNA has been reported to be useful in the diagnosis of multiple myeloma. This study examined whether miR-221 could be used as a diagnostic marker for multiple myeloma. The study was performed on 20 patients with multiple myeloma without any other hematological diseases. MicroRNA extraction was performed using formalin-fixed paraffin-embedded (FFPE) tissues obtained from the bone marrow of patients with multiple myeloma. miR-15a, miR-16, miR-21, miR-181a, and miR-221 were selected as the microRNA target genes for multiple myeloma. The significance of microRNA was based on a fold change of <1.5. To quantify the fold changes, data normalized to the human gene, SNORD43, were used as the values of the patient group. Fold change values greater than 1.5 were defined as "overexpression", whereas values less than -1.5 were defined as "underexpression". Of note, 65.0% (13/20) of samples showed significant "overexpression" in the levels of miR-221 expression and plasma cells with a group of more and less than 30% in MM patients did not show any significance of plasma cell (P<0.05). The results of other studies showing a correlation between the expression of miR-221 and MM in Caucasians were confirmed. These results suggest that miR-221 may be a useful indicator for diagnosing patients with MM. In conclusion, miR-221 is useful in the diagnosis and determining the prognosis of multiple myeloma in Koreans.

Hepatic microRNAome reveals potential microRNA-mRNA pairs association with lipid metabolism in pigs

  • Liu, Jingge;Ning, Caibo;Li, Bojiang;Li, Rongyang;Wu, Wangjun;Liu, Honglin
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.9
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    • pp.1458-1468
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    • 2019
  • Objective: As one of the most important metabolic organs, the liver plays vital roles in modulating the lipid metabolism. This study was to compare miRNA expression profiles of the Large White liver between two different developmental periods and to identify candidate miRNAs for lipid metabolism. Methods: Eight liver samples were collected from White Large of 70-day fetus (P70) and of 70-day piglets (D70) (with 4 biological repeats at each development period) to construct sRNA libraries. Then the eight prepared sRNA libraries were sequenced using Illumina next-generation sequencing technology on HiSeq 2500 platform. Results: As a result, we obtained 346 known and 187 novel miRNAs. Compared with the D70, 55 down- and 61 up-regulated miRNAs were shown to be significantly differentially expressed (DE). Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analysis indicated that these DE miRNAs were mainly involved in growth, development and diverse metabolic processes. They were predicted to regulate lipid metabolism through adipocytokine signaling pathway, mitogen-activated protein kinase, AMP-activated protein kinase, cyclic adenosine monophosphate, phosphatidylinositol 3 kinase/protein kinase B, and Notch signaling pathway. The four most abundantly expressed miRNAs were miR-122, miR-26a and miR-30a-5p (miR-122 only in P70), which play important roles in lipid metabolism. Integration analysis (details of mRNAs sequencing data were shown in another unpublished paper) revealed that many target genes of the DE miRNAs (miR-181b, miR-145-5p, miR-199a-5p, and miR-98) might be critical regulators in lipid metabolic process, including acyl-CoA synthetase long chain family member 4, ATP-binding casette A4, and stearyl-CoA desaturase. Thus, these miRNAs were the promising candidates for lipid metabolism. Conclusion: Our study provides the main differences in the Large White at miRNA level between two different developmental stages. It supplies a valuable database for the further function and mechanism elucidation of miRNAs in porcine liver development and lipid metabolism.

The Influence of Maturity Fears and Appearance Interest on Self-concept among Lower-grade Elementary School Girls with Precocious Puberty (초등학교 저학년 성조숙증 여아의 성숙불안과 신체외모 관심이 자아개념에 미치는 영향)

  • Nam, Hyun-Jeong;Choi, Mi-Young
    • Child Health Nursing Research
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    • v.26 no.2
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    • pp.181-189
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    • 2020
  • Purpose: This study explored the relationships of maturity fears and appearance interest(interest in appearance, interest in weight) with self-concept among girls with precocious puberty. Methods: The participants for this study were 120 elementary school students (8 to 10 years old) and their parents from two hospitals located in Chungcheong Province. Data were collected from January to February 2019 using self-reported questionnaires. The collected data were analyzed using descriptive statistics, the independent t-test, the Mann-Whitney U test, one-way analysis of variance, Pearson correlation coefficients, and multiple regression analysis in SPSS for Windows version 23.0. Results: Statistically significant negative correlations were found between self-concept and maturity fears (r=-.34, p<.001) and interest in weight (r=-.29, p=.001). Maturity fears were identified as a factor affecting the self-concept of girls with precocious puberty (β=-.34, p<.001). This variable explained 11.5% of the variance in the self-concept of girls who experienced precocious puberty. Conclusion: This study confirmed that maturity fears had a major influence on self-concept in girls with precocious puberty. The results of this study can be used as basic data for the development of a psychosocial nursing intervention program for girls who experience precocious puberty.

Relationship of Knowledge and Practical Will to Practice of Cancer Prevention Lifestyle by Late School-aged Children (학령기 후기 아동의 암 예방 생활습관 지식, 실천의지, 실천)

  • Cho, Mi-Hye;Kwon, In-Soo
    • Child Health Nursing Research
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    • v.16 no.4
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    • pp.259-267
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    • 2010
  • Purpose: This study was conducted to identify late school-aged children‘s knowledge, and practical will in the practice of cancer prevention lifestyle, and to examine the relations between these variables and factors that influence practice. Methods: The participants included 525 fifth and sixth grade students from 8 elementary schools in J and S cities. Data were collected in December 2008 using self-report questionnaires developed by the authors and were analyzed using the SPSS program. Results: Mean scores were, for knowledge of cancer prevention lifestyle, $0.76{\pm}0.11$ out of a possible 1, practical will, $4.05{\pm}0.55$ out of a possible 5, and practice, $3.66{\pm}0.41$ also out of 5. There were differences in knowledge (F=4.39, p=.013), practical will (F=18.35, p<.001), and practice (F=29.13, p<.001) according to academic achievement and in practical will (F=3.68, p<.001) and practice (F=3.01, p=.003) according to grade. There were positive correlations between knowledge and practical will (r=.205, p<.001), knowledge and practice (r=.181, p<.001), practical will and practice (r=.628, p<.001). Practice will and knowledge of cancer prevention lifestyle showed a significant influence on practice. Conclusion: Results of this study indicate that effective strategies should be developed to motivate the practical will for the practice of cancer prevention lifestyle during childhood.

Evaluation of marginal fidelity of copy-milled and CAD/CAM all ceramic crowns

  • Jeong Seung-Mi;Kang Dong-Wan;Wolf Christoph
    • The Journal of Korean Academy of Prosthodontics
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    • v.39 no.3
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    • pp.243-249
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    • 2001
  • Statement of the problem. The interest in all-ceramic restorations has increased as more techniques have become available. With the introduction of machinable dental ceramics and CAD/CAM systems or Copy-milling systems there is a need for evaluating the quality levels of these new fabrication techniques. Purpose. This study was to evaluate the fitting accuracy of machined all-ceramic crowns made out of an industrially prefabricated feldspathic porcelain. Material and Methods. Three master models with different cutting depth (0.8mm/1.0mm/1.2mm)were produced using a palladium-silver alloy. A total of 36 working dies, 12 of each form, was used for the modellation of prototype resin copings and 36 additional crowns, 12 of each cutting depth, were produced by using the $CEREC^{(R)}2$ system for all crowns. The maginal fit of all 72 crowns was then evaluated on their respective master die at 54 circularly staggered points of measurement per crown under a fixation pressure of 30 N by using a computerized video image system. Results. The medians of the copy-milled $CELAY^{(R)}$ crowns ranged from 29 to $36{\mu}m$. The highest value for the marginal gap was found in group B (cutting depth 1.0mm) at $107{\mu}m$. The median for the $CEREC^{(R)}2$ crowns was found between 43.5 and $70{\mu}m$. The maximum values for all three groups ranged from $181{\mu}m$ to $286{\mu}m$. With $286{\mu}m$ the highest value for marginal gap was found in group C. the Kruskal-Wallis test and multiple comparisons analysis procedure revealed a significant influence of the production technique on the marginal fit in all three groups (p<0,02). Conclusion. 1. The $CELAY^{(R)}$ system is capable to produce all-ceramic crowns with a significantly better marginal fit than the $CEREC^{(R)}2$ system. 2. As far as premolar crowns produced with the $CEREC^{(R)}2$ system are concerned, the cutting depth has a significant influence on fitting accuracy. 3. The production of crowns with an acceptable marginal fit is possible with both systems. However, adhesive luting is recommended for milled feldspathic porcelain crowns.

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