• Title, Summary, Keyword: mass propagation

Search Result 418, Processing Time 0.046 seconds

Study on the Various Conditions of In Vitro Culture for Mass-propagation of Prunus yedoensis Matsumura (제주(濟州) 자생(自生) 왕벚나무(Prunus yedoensis Matsumura)의 기내(器內) 줄기 증식(增殖)을 위한 배양조건(培養條件) 구명(究明))

  • Cheong, Eun Ju;Kim, Chan Soo
    • Journal of Korean Society of Forest Science
    • /
    • v.90 no.2
    • /
    • pp.184-189
    • /
    • 2001
  • Multiple shoots were induced from in vitro shoot originated from winter bud of P. yedoensis from Jeju. Most explants grow in similar type among the five different media but affected by supplement of sucrose regardless of media. For mass-propagation various concentrations of BAP or $GA_3$ were treated in the medium respectively. BAP was very effective to produce multiple shoots and 3.5~9.5 shoots were formed on the explant. The shoots induced on the high levels of BAP have short internodes. No shoots were induced on the treatment of $GA_3$ but roots were induced on it. When $GA_3$ was supplemented with the medium containing BAP, multiple shoots were produced from the explants. The medium(WPM) containing with $0.5mg/{\ell}$ BAP and $4.0mg/{\ell}$ $GA_3$ was most effective to produce multiple shoots. When the explants were cultured for 8 weeks, 39.5 shoots were developed in average.

  • PDF

Developing a mass propagation technique for Aralia elata via somatic embryogenesis

  • Moon, H.K.;Lee, J.S.;Kim, T.S.
    • Proceedings of the Plant Resources Society of Korea Conference
    • /
    • /
    • pp.114-115
    • /
    • 2000
  • Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will bel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field frill be presented. And some problems arising for the somatic embryogenesis system will be also discussed.

  • PDF

Developing a mass propagation technique for Aralia elata via somatic embryogenesis

  • Moon, H.K.;Lee, J.S.;Kim, T.S.
    • Proceedings of the Plant Resources Society of Korea Conference
    • /
    • /
    • pp.16-17
    • /
    • 2000
  • Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will hel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/ or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field will be presented. And some problems arising for the somatic embryogenesis system will be also discussed.lso discussed.

  • PDF

In Vitro Mass Propagation and Soil Adjastment of Zanthoxylum piperitum var. inerme Makino through Apical Meristem Culture (生長點 培養에 依한 민초피나무(Zanthoxylum piperitum var. inerme Makino)의 器內 大量 增殖 및 土壤 活着)

  • Jeong, Woo-Gyu;Lee, Sang-Rae
    • Korean Journal of Plant Resources
    • /
    • v.6 no.2
    • /
    • pp.171-179
    • /
    • 1993
  • This study was conducted to investigate the effect of growth regulators and medium composition on the growth of each stage in apical meristem culture for mass propagation of Zanthoxylum piperitum var. inerme Makino. The source material, shoot tip segments were taken from three-years old graft trees. Apical meristems were cultured in vitro on basal MS, GD, WS, half strength MS(1/2MS) and half strength GD(1/2GD) media supplemented with various concentrations for growth regulators(BA, IBA) and inorganic nutrients. The results summarized are as follows: 1. In culture establishment stage, ratio of culture establishment was 96.7% and the best resuit was obtained using MS medium supplemented with 1.0mg/l BA and 0.2mg/l IBA. 2. In shoot multitication stage, both shoot multiplication and growth were achieved in average 5.6cm. These results were obtained on in MS medium supplemented with 1.0mg/l BA and 0.2mg/l IBA. 3. In roothing stage, phloroglucinol(PG) acted as IBA synergist in root initiation. The most faverable combinations for root development was half-strength MS medium supplemented with 162mg/l PG and 0.2mg/l IBA, and ratio of rooting was 58.0%. 4. In Vitro formed plantlets were transplanted to paper pots in greenhouse with 85% of relative humidity. 96% of survival rate was obtained from artificial soil mix having same volume of sand, vermiculite, peat, and soil.

  • PDF

Dynamic and wave propagation investigation of FGM plates with porosities using a four variable plate theory

  • Bennai, Riadh;Fourn, Hocine;Atmane, Hassen Ait;Tounsi, Abdelouahed;Bessaim, Aicha
    • Wind and Structures
    • /
    • v.28 no.1
    • /
    • pp.49-62
    • /
    • 2019
  • In this paper, an analytical analysis for the study of vibratory behavior and wave propagation of functionally graded plates (FGM) is presented based on a high order shear deformation theory. The manufacture of these plates' defects can appear in the form of porosity. This latter can question and modify the global behavior of such plates. A new shape of the distribution of porosity according to the thickness of the plate was used. The field of displacement of this theory is present of indeterminate integral variables. The modulus of elasticity and the mass density of these plates are assumed to vary according to the thickness of the plate. Equations of motion are derived by the principle of minimization of energies. Analytical solutions of free vibration and wave propagation are obtained for FGM plates simply supported by integrating the analytic dispersion relation. Illustrative examples are given also to show the effects of variation of various parameters such as(porosity parameter, material graduation, thickness-length ratio, porosity distribution) on vibration and wave propagation of FGM plates.

In Vitro Mass Propagation and Economic Effects of Bioreactor Culture in Ever-bearing Strawberry 'Goha' (Bioreactor를 이용한 사계성 딸기 기내대량증식과 경제성)

  • Lee, Jong-Nam;Kim, Hye-Jin;Kim, Ki-Deog;Kwon, Young-Seok;Im, Ju-Sung;Lim, Hak-Tae;Yeoung, Young-Rok
    • Horticultural Science & Technology
    • /
    • v.28 no.5
    • /
    • pp.845-849
    • /
    • 2010
  • This study was conducted to compare various culture methods and evaluate economic feasibility of each method for mass propagation of new ever-bearing strawberry 'Goha'. Four different methods such as semi-solid culture, solid culture, liquid suspension culture and bioreactor culture were compared. The solid culture and bioreactor culture showed the shortest and longest root length, such as 3.6 cm and 8.3 cm, respectively. Fresh weights of plants cultured in bioreactor were 2,261 mg, which were heavier than those of cultures. Dry weights of plants cultured in bioreactor were the heavier compared to those in other cultures. The number of axillary bud developed in bioreactor was seven, but axillary bud was not developed in other cultures. Production cost through bioreactor culture was calculated to be 303 won per plant which was 542 won less than that of solid culture. As a result, we found that the bioreactor culture was the most cost effective culture method for in vitro mass propagation in new ever-bearing strawberry 'Goha'.

Effect of P Levels in Nutrient solution on the Propagation of Arvuscular Mycorrhizal Funfi in Aeroponics (분무경재배에 의한 arbuscular 균근균 증식에서 양액내 인산농도의 영향)

  • Kim, Young-Ju;Jin, Seo-Yeong;Cho, Ja-Yong;Kim, Kil-Yong;Cha, Gyu-Seok;Soh, Bo-Kyoon
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.37 no.5
    • /
    • pp.350-355
    • /
    • 2004
  • This experiment was carried out to screen the aeroponically grown host plants suitable for the mass propagation of arbuscular mycorrhizal fungi (AMF) inoculum and clarify the effect of P levels in nutrient solution on the growth of aeroponically grown sweet potato (Ipomoea batatas L.), AMF infection, and mass propagation of mycorrhizal spores, etc. Amount of biomass of host plant became higher, as the P levels in nutrient solutions increased from 5 to 20 M. AMF infection rates in mycorrhizal roots increased in higher P levels in nutrient solution, and decreased in lower parts of mycorrhizal roots by about 18.6-26.0%. About 586 mycorrhizal spores per 1 g fresh root were formed at 16 weeks after inoculation of mycorrhizal inoculum. Total of 830,479 mycorrhizal spores were propagated in each plot.

Modelling the coupled fracture propagation and fluid flow in jointed rock mass using FRACOD

  • Zhang, Shichuan;Shen, Baotang;Zhang, Xinguo;Li, Yangyang;Sun, Wenbin;Zhao, Jinhai
    • Geomechanics and Engineering
    • /
    • v.22 no.6
    • /
    • pp.529-540
    • /
    • 2020
  • Water inrush is a major hazard for mining and excavation in deep coal seams or rock masses. It can be attributed to the coalescence of rock fractures in rock mass due to the interaction of fractures, hydraulic flow and stress field. One of the key technical challenges is to understand the course and mechanism of fluid flows in rock joint networks and fracture propagation and hence to take measures to prevent the formation of water inrush channels caused by possible rock fracturing. Several case observations of fluid flowing in rock joint networks and coupled fracture propagation in underground coal roadways are shown in this paper. A number of numerical simulations were done using the recently developed flow coupling function in FRACOD which simulates explicitly the fracture initiation and propagation process. The study has demonstrated that the shortest path between the inlet and outlet in joint networks will become a larger fluid flow channel and those fractures nearest to the water source and the working faces become the main channel of water inrush. The fractures deeper into the rib are mostly caused by shearing, and slipping fractures coalesce with the joint, which connects the water source and eventually forming a water inrush channel.