• Title/Summary/Keyword: liver damage

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A Study on the Effect of Injection Frequency on the Liver Damage in Rats (Bromobenzene의 투여 횟수에 따른 간독성의 차이)

  • 이상희;전태원;윤종국
    • Biomedical Science Letters
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    • v.6 no.1
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    • pp.29-36
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    • 2000
  • To investigate the effect of injection frequency of bromobenzene on the liver damage, bromobenzene (400 mg/kg, i.p.) was given daily to rats for six days. All experimental animals were sacrificed at 24 hours after the last injection. Morphological changes of the liver were observed under a light microscopic examination. Functional changes of the liver were evaluated by the measurement of alanine aminotransferase activity. To clarify the cause of discrepancy in liver damage, hepatic glutathione (GSH) content, glutathione S-transferase (GST) and aniline hydroxylase (AH) activities were determined. In the experiments of daily bromobenzene treatments, the sacrificed animals at six day (6 time-injected animals) showed slighter liver damage than those sacrificed at 3 day (3 time-injected ones), based on the liver morphological or functional findings; the decreasing ratio of GSH content and increasing ratio of liver GST and AH activities in the 6 time-injected group were higher than those in the 3 time-injected one.

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Comparison of Liver Damage in Bromobenzene-Daily Treated Rats with Every Other Day Treated Ones (랫드에 있어서 Bromobenzene의 격일 투여 시, 매일 투여한 경우와 간손상 정도의 비교)

  • 이상희;윤종국;조현국
    • Biomedical Science Letters
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    • v.6 no.2
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    • pp.101-107
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    • 2000
  • To evaluate the effect of intervals of bromobenzene treatment on the liver damage, the bromobenzene (400 mg/kg, i.p.) was given to rats at either one day or two days interval at three times. All the experimental animals were sacrificed at 24 hours after the last injection. Liver morphological changes were observed under a light microscopic examination and liver functional changes were determined by the measurement of alaine aminotransferase (ALT) activity and hepatic malondialdehyde (MDA) content. The experimental to examine the cause of liver damage were cytochrome P45O, glutathione (GSH) content and glutathione S-transferase (GST) activities. The results are summarized as follows; Based on the liver morphological and functional findings, the daily bromobenzene-treated rats (ED) showed the more severe liver damage than every other day bromobenzene-treated rats (EOD). The hepatic cytochrome P45O content was higher in EOD group than that in ED group. And the increasing rate of hepatic GST activity and decreasing rate of GSH content to the control were higher in EOD group than that in ED group. In conclusion, the treatment of bromobenzene intermittently to the rats may lead to more reduced liver injury compared with the continuously treated animals when both cases are treated with the same dose and frequency, and it may be caused by the enhancement of bromobenzene metabolism.

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Effect of Liver Protection of Garlic on Acute Liver Damage Caused by Carbon tetrachloride (사염화탄소에 의해 유발된 급성간장해에 대한 마늘의 간장 보호 효과)

  • 박무현
    • Korean Journal of Plant Resources
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    • v.8 no.3
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    • pp.275-280
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    • 1995
  • To determine the effect of garlic on acute liver damage caused by $CCl_4$, the values of GOT, GPT, LDH in blood were measured. GOT, GPT, LDH values from mouse treated 2,000mg/kg garlic substance powder were significantly lower than disitlled mouse(positive treatment) after distilled water ingestion only showed big death, but 2,000mg/kg garlic powder treated mouse rarely showed death of liver tissue.

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Inhibition Effects of Auricularia auricula-judae Methanol Extract on Lipid Peroxidation and Liver Damage in Benzo(a)pyrene-Treated Mice (목이버섯 메탄올 추출물이 벤조피렌(B(a)P) 투여한 마우스의 지질과산화 및 간 손상 억제에 미치는 영향)

  • 이갑랑;장종선;김현정;배준태;박선희;이승언;김옥미
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.4
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    • pp.712-717
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    • 1998
  • This study was undertaken to investigate the inhibition effects of Auricularia auricula-judae methanol extract in edible mushroom on lipid peroxidation and liver damage in benzo(a)pyrene(B(a)P)-treated mice. The activities of serum aminotransferase, cytochrome P-450, superoxide dismutase, catalase, glutathione peroxidase and the hepatic content of lipid peroxide after B(a)P-treatment was markedly increased than control but those levels were significantly decreased by the treatment of Auricularia auricula-judae methanol extract. Glutathione S-transferase activity and the hepatic glutathione content were decreased by B(a)P-treatment than control, but those were also inhibited by the treament of Auricularia auricula-judae methanol extract. These results suggest that Auricularia auricula-judae methanol extract have a protective effect on liver damage by B(a)P.

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Effect of Solanum lyratum Extract on Dimethylniatrosamine-Induced Liver Damage in Rats (Dimethylnitrosamine에 의해 유도된 흰쥐 간독성에 대한 배풍등 추출물의 보호효과)

  • Shin, Mi-Ok;Park, Jong-Hee;Moon, Jeon-Ok
    • Korean Journal of Pharmacognosy
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    • v.34 no.1
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    • pp.60-64
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    • 2003
  • Solanum lyratum(Solanaceae) has been used as a traditional analgesic, antipyretic and hepatoprotective agents in Korea. In this study, we investigated the hepatoprotective effect of ethylacetate extract of Solanum lyratum (SL) on the dimethylnitrosamine (DMN)-induced liver damage in rats. Oral administration of SL (150, 300 mg/kg daily for 4 weeks) into the DMN-treated rats remarkably prevented the elevation of serum alanine transaminase, aspartate transaminase and alkaline phosphatase levels. SL also increased serum protein level and reduced the hepatic level of malondialdehyde in DMN-treated rats. Furthermore, DMN-induced elevation of hydroxyproline content was reduced by the treatment of SL. In conclusion, these results demonstrated that SL exhibited in vivo hepatoprotective effect against DMN-induced liver injury, and suggest that SL may be useful in the prevention of liver damage.

Effects of Sachunghwan Extract on Liver Damage in Thioacetamide Induced Rats (사청환(瀉靑丸)이 thiaocetamide에 의한 백서(白鼠) 간손상(肝損傷)에 미치는 영향)

  • Chai, Jung-Won
    • The Journal of Pediatrics of Korean Medicine
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    • v.21 no.2
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    • pp.89-96
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    • 2007
  • Objectives The purpose of this study is to examine of the effect of Sachunghwan on the liver disease. Methods It was induced liver damage rats by thioacetamide and dosed the extract orally and measured the activity rate of GOT, GPT, ALP and LDH. Results 1. GOT activity was more decreased in the experimental goup than in the control goup. Group I showed continuous significance after 72 hour, Group II was significance after 96 hours. 2. GPT activity was more decreased in the experimental group than in the control group. Group I was effective after 72 hours, but group II was effective after 96 hours. 3. The significance of ALP activity in the Group I and group II was revealed after 72 hours. 4. The significance of LDH activity in the Group I and group II was revealed after 96 hours. Conclusions It was showded that Sachunghwan extract was effective on liver disease.

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Hepatoprotective activity of methanol extracts of Glinus oppositifolius and Trianthema decandra against paracetamol induced liver damage

  • Gupta, M;Mazumder, UK;Haldar, PK;Manikandan, L;Senthilkumar, GP;Kandar, CC
    • Advances in Traditional Medicine
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    • v.7 no.1
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    • pp.74-78
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    • 2007
  • The plants, Trianthema decandra and Glinus oppositifolius are commonly used by tribal people in India for the treatment of liver diseases. Hepatoprotective activity of methanol extracts of Glinus oppositifolius and Trianthema decandra at the dose of 250 and 500 mg/kg body weight administered orally was evaluated against paracetamol induced liver damage in rats. Biochemical parameters such as serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, serum alkaline phosphatase, bilirubin, total serum protein, lipid peroxide and glutathione content of the liver were estimated to determine liver function and metabolism. From the biochemical observations, it was concluded that methanol extracts of Glinus oppositifolius and Trianthema decandra significantly restored the altered biochemical parameters towards normal condition in paracetamol induced liver damage.

Effects of Low Level Laser Treatment at LR2 and LR8 acupoint on the liver damage induced in D-GalN in rats (간경(肝經)의 형화혈(滎火穴)과 합수혈(合水穴)에 시술한 레이저침이 D-GalN 간손상 유발 흰쥐에 미치는 영향)

  • Kim, Wang-In;Youn, Dae-Hwan;Choi, Chan-Hun;Na, Chang-Su
    • Korean Journal of Acupuncture
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    • v.29 no.1
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    • pp.131-141
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    • 2012
  • Objectives : This study was performed to investigate the effect of invasive laser acupuncture treatment at Liver Brook (LR2) acupoint and Liver Sea (LR8) acupoint on liver damage induced by D-galactosamine (D-GalN) in rats Methods : Liver damage was induced by D-GalN. The experimental rats were divided into two groups (control group, Low Level Laser Treatment (LLLT) group). Control groups were classified into small groups. Intact group had no liver damage and no treatment. D-GalN group was induced liver damage induced by D-GalN and not treated. LLLT group were induced liver damage induced by D-GalN and then treated at the LR2 or LR8 acupoint with 532, 658, 904 nm invasive laser acupuncture. The treatment was carried out three days at a time for 15days at both acupoints. To examine mechanism of the effect of invasive laser acupuncture, we measured the contents of ASP, ALT, ALP, TBIL in serum, CBC in blood and SOD in liver tissue. Results : The change of body weight increased in all groups. That change was AST and ALP, the AST activity decreased significantly compared with the control groups and decreased by 532 nm and 904 nm both LLLT groups. But ALP increased at LR8 acupoint by 658 nm. TBIL level significantly decreased in all LLLT groups. The SOD of LLLT groups increased in the liver tissue of rats compared to the control groups. SOD activity indicated that LLLT can help cellular defense mechanism by preventing scavenging hydrogen peroxide. In the change of WBC, it was increased in D-GalN Control group compared to intact group and LLLT groups. Conclusions : These results suggested that invasive laser acupuncture treatment at LR2 or LR8 acupoint reduced activation of hepatic enzyme and damage of liver tissue. Thus, the effect of invasive laser acupuncture was nearly identical to the way of the traditional acupuncture for the treatment of hepatocytotoxicity.

Effects of Doxapram Hydrochloride on the Sedative Action of Xylazine Hydrochloride in Goats with Carbon Tetrachloride Induced Liver Damage (사염화탄소로 저손상을 일으킨 산양에서 Doxapram Hydrochloride가 Xylazine Hydrochloride의 진정작용에 미치는 영향)

  • Cheong Kwang-Eop;Cheong Chang-Kook
    • Journal of the korean veterinary medical association
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    • v.19 no.10
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    • pp.29-36
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    • 1983
  • The sedative action of xylazine hydrochloride and effects of doxapram hydrochloride on the sedative action of xylazine hydrochloride were investigated in goats with carbon tetrachloride induced liver damage. The results obtained were as follows. 1. Sedati

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The Protective Effects of Scutellaria baicalensis Georgi Water Extracts on the Immunomodulatory Effects on Liver Damage Induced by Carbon Tetrachloride in Rats (사염화탄소로 간 손상이 유발된 흰쥐에서 황금(黃芩) 열수 추출물이 면역작용에 미치는 효과)

  • An, Chi-Sun;Jin, Hai-Lan;Jeon, Yun-Hui;Lim, Beong-Ou
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.4
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    • pp.273-279
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    • 2009
  • GATA-binding protein-3 (GATA-3) and T-box expressed in T-cells (T-bet) are now considered as master transcription factors involving Th cell differentiation, but the roles of these factors are still uncertain in vivo. This study was conducted to investigate the expression of these transcription factors in the liver damage induced by carbon tetrachloride ($CCl_4$) in rats. In this study, liver damage were induced with Scutellaria baicalensis Georgi water extracts (SBW) and followed for 4 weeks. The expression of GATA-3 and T-bet protein in liver damage induced by $CCl_4$ and the serum levels of immunoglobulin A (IgA), IgE were studied after 4 weeks of treatment. We found that effect of SBW on IFN-$\gamma$, STAT1, pSTAT1 and T-bet was decreased in vivo. Several genes were demonstrated to be IL-4 inducible prior to the discovery of STAT6. $CCl_4$+SBW group was significantly lower than $CCl_4$ group in IL-4, STAT6, pSTAT6 and GATA-3. Our data indicate that cytokine protein production were increased in $CCl_4$ group and $CCl_4$+SBW group. From these results, water extracts obtained from Scutellaria baicalensis Georgi may have an immunoregulatory effect in the liver induced by $CCl_4$ of rats.