• Title, Summary, Keyword: liver

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Experimental Applications of in situ Liver Perfusion Machinery for the Study of Liver Disease

  • Choi, Won-Mook;Eun, Hyuk Soo;Lee, Young-Sun;Kim, Sun Jun;Kim, Myung-Ho;Lee, Jun-Hee;Shim, Young-Ri;Kim, Hee-Hoon;Kim, Ye Eun;Yi, Hyon-Seung;Jeong, Won-Il
    • Molecules and Cells
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    • v.42 no.1
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    • pp.45-55
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    • 2019
  • The liver is involved in a wide range of activities in vertebrates and some other animals, including metabolism, protein synthesis, detoxification, and the immune system. Until now, various methods have been devised to study liver diseases; however, each method has its own limitations. In situ liver perfusion machinery, originally developed in rats, has been successfully adapted to mice, enabling the study of liver diseases. Here we describe the protocol, which is a simple but widely applicable method for investigating the liver diseases. The liver is perfused in situ by cannulation of the portal vein and suprahepatic inferior vena cava (IVC), with antegrade closed circuit circulation completed by clamping the infrahepatic IVC. In situ liver perfusion can be utilized to evaluate immune cell migration and function, hemodynamics and related cellular reactions in each type of hepatic cells, and the metabolism of toxic or other compounds by changing the composition of the circulating media. In situ liver perfusion method maintains liver function and cell viability for up to 2 h. This study also describes an optional protocol using density-gradient centrifugation for the separation of different types of hepatic cells, allowing the determination of changes in each cell type. In summary, this method of in situ liver perfusion will be useful for studying liver diseases as a complement to other established methods.

Analysis on Usefulness of Non-invasive Liver Fibrosis Evaluation Method according to the Liver Disease : Focused on Hepatitis C patients (간질환 종류에 따른 비침습적 간섬유화 평가법의 유용성 분석 : C형 간염 보균자 중심으로)

  • Nam, Ji-Hee;Kim, Jung-Hoon
    • Journal of radiological science and technology
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    • v.42 no.5
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    • pp.345-350
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    • 2019
  • Liver biopsy is the gold standard for diagnosing liver fibrosis, but it is invasive and has a risk for complications. For this reason, recently, study has been actively conducted on non-invasive liver fibrosis evaluation method. But, there is no established standard for the type of diffuse liver disease. Therefore, this study was suggest the usefulness and cut-off values of Fibroscan, FIB-4, APRI and AAR of patients with hepatitis C in Korea. According to the diagnosis, 240 people in hepatitis C are classified into fatty liver, chronic hepatitis, and liver cirrhosis. The statistical analysis was performed by ANOVA to verify difference between groups. The ROC curve was analyzed to determine the usefulness and practical cut-off value. As a result, for all diseases, the AUC value for Fibroscan was 0.8 over and the APRI was 0.7 over. Cut-off value of serum based liver fibrosis markers was increased in order of fatty liver, chronic hepatitis and liver cirrhosis. If Fibroscan and serological liver fibrosis markers are applied to predict liver fibrosis, it is expected that excessive liver biopsy can be reduced.

An Association between Liver Markers and Physiological Variables: Comparison between Normal and Fatty Liver Subjects

  • Hyun, Kyung-Yae
    • Biomedical Science Letters
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    • v.17 no.2
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    • pp.105-112
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    • 2011
  • We evaluated whether liver markers such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyltranspeptidase (GGT), and bilirubin have a relationship with other physiological factors in the normal (n=115) and fatty liver subjects (n=122) and there are differences between the two populations. Body indices were higher in the fatty liver group than in the normal group. Liver markers and blood pressure (BP) were greater in the fatty liver group than in the normal group. AST and ALT levels were positively correlated with body indices in the fatty liver group, but not in the normal group. AST, ALT and GGT levels in the fatty liver group had positive relationship with cardiovascular indices (CI). ALP and bilirubin levels were negatively associated with some of CI. Liver markers were negatively or positively correlated with inflammatory markers, thyroid hormones, or several biochemical markers levels. These findings suggest that abnormal changes in liver markers may be useful tool for diagnosis or prognosis of development of cardiovascular and/or inflammatory diseases as well as metabolic syndrome.

Evaluation of Stage of Liver Fibrosis by Ultrasonography : Based on Pathologic Results of Biopsy (초음파검사를 통한 간 섬유화 병기단계 평가 : 조직검사결과 기준으로)

  • An, Hyun;Lee, Hyo-Yeong;Im, In Chul
    • Journal of the Korean Society of Radiology
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    • v.13 no.4
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    • pp.547-555
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    • 2019
  • The purpose of this study was to evaluate the usefulness of routine liver ultrasonography on the basis of the scoring system according to the morphological parameters of liver ultrasound images and the histopathological results of liver biopsy. The morphological parameters of the liver through ultrasonography were divided into liver surface, liver edge and liver parenchyma. Pathologic results of liver biopsy were classified as mild fibrosis(F1), significant fibrosis(F2), severe fibrosis(F3), and cirrhosis(F4). In conclusion, routine ultrasound examination showed a sensitive predictive factor for fibrosis with mild fibrosis (F1) to severe fibrosis (F3) were liver edge>liver parenchyma>liver surface. However, the predictive factors for detecting cirrhosis (F4) were liver parenchyma>liver surface>liver edge. The use of three variable combinations rather than individual variables in routine ultrasonography may be useful in evaluating the degree and progress of liver fibrosis.

Effects of zolpidem on sleep parameters in patients with cirrhosis and sleep disturbances: A randomized, placebo-controlled trial

  • Sharma, Manoj Kumar;Kainth, Sumeet;Kumar, Sachin;Bhardwaj, Ankit;Agarwal, Hemant Kumar;Maiwall, Rakhi;Jamwal, Kapil Dev;Shasthry, Saggere Muralikrishna;Jindal, Ankur;Choudhary, Ashok;Anand, Lovkesh;Dhamija, Rajender Mal;Kumar, Guresh;Sharma, Barjesh Chander;Sarin, Shiv Kumar
    • Clinical and Molecular Hepatology
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    • v.25 no.2
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    • pp.199-209
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    • 2019
  • Background/Aims: The aim of this study was to study the efficacy and safety of zolpidem for sleep disturbances in patients with cirrhosis. Methods: Fifty-two Child-Turcotte-Pugh (CTP) class A or B cirrhotics with Pittsburgh Sleep Quality Index >5 were randomized to either zolpidem 5 mg daily (n=26) or placebo (n=26) for 4 weeks. Results: The therapy of 4 weeks was completed by 23 patients receiving zolpidem (3 stopped treatment due to excessive daytime drowsiness) and 24 receiving placebo (2 refused to continue the study). In the zolpidem group, after 4 weeks of therapy, there was significant increase in total sleep time (TST) and sleep efficiency compared to baseline and improvement in polysomnographic parameters of sleep initiation and maintenance (i.e., decrease in sleep latency time, decrease in wake time, and decreases in number of arousals and periodic limbs movements per hour of sleep), without any significant change in sleep architecture. Conclusions: Four weeks of 5 mg daily zolpidem in CTP class A or B cirrhosis patients with insomnia led to significant increases in TST and sleep efficiency and improvement in polysomnographic parameters of sleep initiation and maintenance without any significant change in sleep architecture.

The Jang suk-sun's explanation and clinical application about liver (장석순의 간에 대한 천발과 임상응용)

  • 김진옥;김용진
    • The Journal of Korean Medicine
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    • v.25 no.1
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    • pp.85-95
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    • 2004
  • The theory of liver by Jang suk-sun[張錫純] is that first, although liver exists at right side of body and spleen at left anatomically, function of liver presents at left side of body and function of spleen at right based on principle of 'interdependence between eum and yang' and 'join strength with elasticity', and in the relation between liver and spleen, if gi of spleen ascends, gi of liver also ascends, and if gi of stomach descents, gi of gall bladder also descents. So. care of spleen and stomach becomes main point in treating disease of liver. The meaning of 'the liver being in charge of the evaporation'[肝主氣化] is that first, it raises the primordial gi and forms the 'great g' for it's circulation of whole body. Second., it excretes the functional activity of gi and connects heart with kidney and guides the evaporation of the whole body by connecting innate nature with acquired nature. Third, 'the liver being in charge of the evaporation' is realized by the help of spleen and stomach. And he said that this functional activity of gi is one of distinctive features that distinguish Oriental medicine from Western medicine. He discoursed upon physiology of 'the liver being in charge of the evaporation' and 'the Body belonged to yin and the Use belonged to yang' after seeing an evil of abusing drugs that calm the liver and check hyperfunction of liver by contemporary doctors. And he established a treat of 'warming and recuperating the gi of liver' [溫補肝氣法] and used it for symptoms of 'deficiency of liver-gi'[肝氣虛], 'exhaution of liver-gi'[肝氣脫], and 'the liver-cold'[肝寒證].

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A Study on the Classification of Ultrasonic Liver Images Using Multi Texture Vectors and a Statistical Classifier (다중 거칠기 벡터와 통계적 분류기를 이용한 초음파 간 영상 분류에 관한 연구)

  • 정정원;김동윤
    • Journal of Biomedical Engineering Research
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    • v.17 no.4
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    • pp.433-442
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    • 1996
  • Since one texture property(i.e coarseness, orientation, regularity, granularity) for ultrasound liver ages was not sufficient enough to classify the characteristics of livers, we used multi texture vectors tracted from ultrasound liver images and a statistical classifier. Multi texture vectors are selected among the feature vectors of the normal liver, fat liver and cirrhosis images which have a good separability in those ultrasound liver images. The statistical classifier uses multi texture vectors as input vectors and classifies ultrasound liver images for each multi texture vector by the Bayes decision rule. Then the decision of the liver disease is made by choosing the maximum value from the averages of a posteriori probability for each multi texture vector In our simulation, we obtained higtler correct ratio than that of other methods using single feature vector, for the test set the correct ratio is 94% in the normal liver, 84% in the fat liver and 86% in the cirrhosis liver.

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Diffusion-Weighted Imaging for the Left Hepatic Lobe has Higher Diagnostic Accuracy for Malignant Focal Liver Lesions

  • Han, Xue;Dong, Yin;Xiu, Jian-Jun;Zhang, Jie;Huang, Zhao-Qin;Cai, Shi-Feng;Yuan, Xian-Shun;Liu, Qing-Wei
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.15
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    • pp.6155-6160
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    • 2014
  • Background: This study was conducted to investigate whether apparent diffusion coefficient (ADC) measurements by dividing the liver into left and right hepatic lobes may be utilized to improve the accuracy of differential diagnosis of benign and malignant focal liver lesions. Materials and Methods: A total of 269 consecutive patients with 429 focal liver lesions were examined by 3-T magnetic resonance imaging that included diffusion-weighted imaging. For 58 patients with focal liver lesions of the same etiology in left and right hepatic lobes, ADCs of normal liver parenchyma and focal liver lesions were calculated and compared using the paired t-test. For all 269 patients, ADC cutoffs for focal liver lesions and diagnostic accuracy in the left hepatic lobe, right hepatic lobe and whole liver were evaluated by receiver operating characteristic curve analysis. Results: For the group of 58 patients, mean ADCs of normal liver parenchyma and focal liver lesions in the left hepatic lobe were significantly higher than those in the right hepatic lobe. For differentiating malignant lesions from benign lesions in all patients, the sensitivity and specificity were 92.6% and 92.0% in the left hepatic lobe, 94.4% and 94.4% in the right hepatic lobe, and 90.4% and 94.7% in the whole liver, respectively. The area under the curve of the right hepatic lobe, but not the left hepatic lobe, was higher than that of the whole liver. Conclusions: ADCs of normal liver parenchyma and focal liver lesions in the left hepatic lobe were significantly higher than those in the right hepatic lobe. Optimal ADC cutoff for focal liver lesions in the right hepatic lobe, but not in the left hepatic lobe, had higher diagnostic accuracy compared with that in the whole liver.

The Study on Acceptability of Liver Sausage by Proportions of Liver and Processings (간함량비율과 제조방법에 따른 간소시지의 기호도에 관한 연구)

  • Jo, Jeong-Sun;Lee, Suk-Mi
    • Journal of the Korean Dietetic Association
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    • v.3 no.1
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    • pp.90-100
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    • 1997
  • Objectives of this study were to investigate the acceptability of liver sausages, one of the most favorite product of meats, and to examine the applicability of pork liver in manufacturing sausages. Sausages containing different proportions of pork liver were manufactured with various processes and were tested and analysed by means of sensory evaluation. Based on th basic recipe for manufacturing sausages, fifteen recipies were proposed with different combinations of ingredients, followed by evaluations on their physicochemical and nutritional properties. Sausages manufactured with different methods (sliceable, spreadable, smoked spreadable) and different proportions of liver were also evaluated. The Findings from these experiments are summarized as follows: 1. As the percentage of the liver was increased, outer color of experimental sausages were significantly decreased. 2. The hardness, gumminess, chewiness were significantly decreased as the amount of liver increased. 3. Sausages containing 30% pork liver had best acceptability after swallowing. 4. The flavor and taste of liver in experimental sausages were significantly increased by adding liver. In summary, sausages containing 30% pork liver had best acceptability for consumption in terms of the indices measured in this study.

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