• Title, Summary, Keyword: lichen

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Leiorreuma exaltatum and Trapelia coarctata, New to Korean Lichen Flora

  • Joshi, Santosh;Jayalal, Udeni;Lokos, Laszlo;Park, Jung Shin;Oh, Soon-Ok;Koh, Young Jin;Hur, Jae-Seoun
    • Mycobiology
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    • v.41 no.1
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    • pp.56-58
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    • 2013
  • The present account briefly describes two crustose lichen species new to South Korea. Detailed taxonomic descriptions of Leiorreuma exaltatum and Trapelia coarctata, supported by distribution, ecology and illustrations, are provided. Both species were collected from warm-temperate, humid forests of Jeju Island.

First Report of the Lichen Species, Heterodermia flabellata (F$\acute{e}$e) D. D. Awasthi, and Updated Taxonomic Key of Heterodermia in South Korea

  • Jayalal, Udeni;Joshi, Santosh;Oh, Soon-Ok;Park, Jung-Shin;Hur, Jae-Seoun
    • Mycobiology
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    • v.40 no.3
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    • pp.202-204
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    • 2012
  • Heterodermia flabellata (F$\acute{e}$e) D. D. Awasthi was found as a new lichen record in Jeju Island of South Korea in 2012. A detailed taxonomic description and comments are provided for the taxa. An updated key for all recorded species of Heterodermia from South Korea is given.

An Easy, Rapid, and Cost-Effective Method for DNA Extraction from Various Lichen Taxa and Specimens Suitable for Analysis of Fungal and Algal Strains

  • Park, Sook-Young;Jang, Seol-Hwa;Oh, Soon-Ok;Kim, Jung A;Hur, Jae-Seoun
    • Mycobiology
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    • v.42 no.4
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    • pp.311-316
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    • 2014
  • Lichen studies, including biodiversity, phylogenetic relationships, and conservation concerns require definitive species identification, however many lichens can be challenging to identify at the species level. Molecular techniques have shown efficacy in discriminating among lichen taxa, however, obtaining genomic DNA from herbarium and fresh lichen thalli by conventional methods has been difficult, because lichens contain high proteins, polysaccharides, and other complex compounds in their cell walls. Here we report a rapid, easy, and inexpensive protocol for extracting PCR-quality DNA from various lichen species. This method involves the following two steps: first, cell breakage using a beadbeater; and second, extraction, isolation, and precipitation of genomic DNA. The procedure requires approximately 10 mg of lichen thalli and can be completed within 20 min. The obtained DNAs were of sufficient quality and quantity to amplify the internal transcribed spacer region from the fungal and algal lichen components, as well as to sequence the amplified products. In addition, 26 different lichen taxa were tested, resulting in successful PCR products. The results of this study validated the experimental protocols, and clearly demonstrated the efficacy and value of our KCl extraction method applied in the fungal and algal samples.

Plant Hormones Promote Growth in Lichen-Forming Fungi

  • Wang, Xin Yu;Wei, Xin Li;Luo, Heng;Kim, Jung-A;Jeon, Hae-Sook;Koh, Young-Jin;Hur, Jae-Seoun
    • Mycobiology
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    • v.38 no.3
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    • pp.176-179
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    • 2010
  • The effect of plant hormones on the growth of lichen-forming fungi (LFF) was evaluated. The use of 2,3,5-triiodobenzoic acid and indole-3-butyric acid resulted in a 99% and 57% increase in dry weight of the lichen-forming fungus Nephromopsis ornata. The results suggest that some plant hormones can be used as inducers or stimulators of LFF growth for large-scale culture.

The Lichen Dirinaria picta New to South Korea

  • Jayalal, Udeni;Oh, Sang-Sil;Joshi, Santosh;Oh, Soon-Ok;Hur, Jae-Seoun
    • Mycobiology
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    • v.41 no.3
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    • pp.155-158
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    • 2013
  • Two species of the lichen genus Dirinaria (Tuck.) Clem. are recognized in the South Korean lichen mycota, viz: D. applanata and D. picta. Descriptions of each species with their morphological, anatomical, and chemical characteristics together with molecular analysis and a key to known Dirinaria species are presented. D. picta is recorded from South Korea for the first time.

New Additions to Lichen Mycota of the Republic of Korea

  • Joshi, Santosh;Kondratyuk, Sergey Y.;Crisan, Florin;Jayalal, Udeni;Oh, Soon-Ok;Hur, Jae-Seoun
    • Mycobiology
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    • v.41 no.4
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    • pp.177-182
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    • 2013
  • The current study describes seven species that are new to the lichen mycota of South Korea. A taxonomic description of Arthonia excipienda, A. radiata, Arthothelium ruanum, Enterographa leucolyta, Fissurina elaiocarpa, Rinodina oleae, and Thelotrema porinaceum was given and supported by distribution, ecology, and illustrations. Each species was compared with a species showing close resemblance.

New Records and an Annotated Key for the Identification of Graphis Adans. in South Korea

  • Joshi, Santosh;Jayalal, Udeni;Oh, Soon-Ok;Park, Jung Shin;Hur, Jae-Seoun
    • Mycobiology
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    • v.41 no.2
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    • pp.73-76
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    • 2013
  • The following new species for the lichen genus Graphis in Korea are reported: G. chlorotica, G. nanodes and G. tenuirima. A brief description of these species, together with their distribution, ecology, and illustrations are provided. A key to all known species of this genus from Korea is also presented.

Lichen Mycota in South Korea: The Genus Usnea

  • Jayalal, Udeni;Joshi, Santosh;Oh, Soon-Ok;Koh, Young Jin;Crisan, Florin;Hur, Jae-Seoun
    • Mycobiology
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    • v.41 no.3
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    • pp.126-130
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    • 2013
  • Usnea Adans. is a somewhat rare lichen in South Korea, and, in nearly two decades, no detailed taxonomic or revisionary study has been conducted. This study was based on the specimens deposited in the lichen herbarium at the Korean Lichen Research Institute, and the samples were identified using information obtained from recent literature. In this study, a total of eight species of Usnea, including one new record, Usnea hakonensis Asahina, are documented. Detailed descriptions of each species with their morphological, anatomical, and chemical characteristics are provided. A key to all known Usnea species in South Korea is also presented.

The Lichen Genus Parmotrema in South Korea

  • Jayalal, Udeni;Divakar, Pradeep K.;Joshi, Santosh;Oh, Soon-Ok;Koh, Young Jin;Hur, Jae-Seoun
    • Mycobiology
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    • v.41 no.1
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    • pp.25-36
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    • 2013
  • Parmotrema A. Massal. is a common lichen genus scattered throughout the Korean Peninsula; however, no detailed taxonomic or revisionary study of this genus has been conducted for nearly two decades. Therefore, this study revised the taxonomy of this genus based on specimens deposited in the lichen herbarium at the Korean Lichen Research Institute and samples wereidentified using recent literature. In this revisionary study, a total of eighteen species of Parmotrema including eight new records [Parmotrema cetratum (Ach.) Hale, Parmotrema cristiferum (Taylor) Hale, Parmotrema grayanum (Hue) Hale, Parmotrema defectum (Hale) Hale, Parmotrema dilatatum (Vain.) Hale, Parmotrema margaritatum (Hue) Hale, Parmotrema pseudocrinitum (Abbayes) Hale, and Parmotrema subsumptum (Nyl.) Hale] are documented. Detailed descriptions of each species with their morphological, anatomical and chemical characteristics are also given and a key to the known Parmotrema species of the Korean Peninsula is presented.

Notes on the Lichen Genus Hypotrachyna (Parmeliaceae) from South Korea

  • Jayalal, Udeni;Joshi, Santosh;Oh, Soon-Ok;Park, Jung-Shin;Koh, Young Jin;Hur, Jae-Seoun
    • Mycobiology
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    • v.41 no.1
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    • pp.13-17
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    • 2013
  • Hypotrachyna (Vainio) Hale is a somewhat rare lichen genus found on the Korean Peninsula. Since it was first recorded more than two decades ago, no detailed taxonomic or revisionary study of the genus has been conducted. Thus, the present study was conducted to carry out a detailed taxonomic and revisionary study of Hypotrachyna in South Korea. This study was based on specimens deposited in the Korean Lichen Research Institute (KoLRI). Detailed taxonomic studies and a literature review confirmed the presence of seven species of Hypotrachyna from South Korea, including one new record, Hypotrachyna nodakensis (Asahina) Hale. Descriptions of each species with their morphological, anatomical and chemical characters together with a key to all known Hypotrachyna species are presented.