• Title, Summary, Keyword: kidney bean seedling

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Biochemical Characterization of Lectin Purified from Kidney Bean Seedling (강낭콩 유식물로부터 분리한 Lectin의 생화학적 특성)

  • Roh, Kwang-Soo
    • KSBB Journal
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    • v.22 no.1
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    • pp.53-57
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    • 2007
  • We have studied biochemical characterization of lectin purified from kidney bean seedling through PBS extraction, $(NH_4)_2SO_4$ precipitation, and Sephadex G-100 affinity chromatography. The lectin was agglutinated by rabbit erythrocytes. This lectin analyzed by SDS-PAGE is a tetramer composed of two subunits with molecular weights of 46 and 44 kDa. The optimal temperature and thermal stability of the lectin was 30$^{\circ}C$ and 40-80$^{\circ}C$, respectively. The maximal pH of this lectin was pH 8.2.

Glutathione Content in Various Seedling Plants, Vegetables, and the Processed Foods (각종 유식물체, 채소 및 가공식품 중의 글루타치온 함량)

  • Kim, Ju-Sung;Shim, Ie-Sung;Kim, Myong-Jo
    • Korean Journal of Food Science and Technology
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    • v.41 no.5
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    • pp.592-596
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    • 2009
  • In this study, we investigated the levels of glutathione (GSH) and its oxidized form (GSSG) in more than 40 kinds of plant materials including seedling plants, grains, vegetables, and processed foods. The glutathione contents in the seedling plants were ranged from 0 to $120{\mu}mol/100g$. In addition, the different levels of glutathione were observed within the same family and between species. In the case of marketed grains and vegetables, azuki and kidney beans of leguminosae contained the high levels of glutathione, whereas glutathione was scarcely detected in the processed bean foods (bean paste, soybean sauce, etc.). Overall, a higher GSH content in food may contribute to a higher added value.

Variation of Sugar Content in Different Parts of Seedling during Germination in Phaseolus vulgaris (강낭콩 발아중 유묘 부위별 당함량의 변화)

  • 김종진
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    • v.41 no.4
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    • pp.482-488
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    • 1996
  • The experiment was carried out to the find variation of the sugar contents in the tip and basal part of the cotyledon and remaining portion of embryos in Phaseolus vulgahs seeds during germination with HPLC analysis method. Water content in cotyledon of kidney bean seed was about 6.4~6.5 of fresh weight and incresed to 45.8~71.2% during germination showing that tip part of cotyledon has more water content by 1.1~3.8% compared to the lower part of it. Higher water onten was observed in the rest parts of the seed except cotyledon such as plumule, radicle and hypocotyl showing that it increased to 72.2~93.3% depending on the different tissue organs. Main important sugars in kidney seeds during germination stages are; raffinose, sucrose, glucose and fructose, and the amount are differed with the kinds of embryo in kidney bean seed organs and stages of germination. Raffinose amount in kidney bean is increasing repeatly when seeds become wet but disappear it soon after seed have germinated especially in growing embryo parts. Raffinose in basal parts of cotyledons were still presented some an amount after germination. Sucrose is synthesized highly in plumule embryo at the beginning stage of germination but disappeared it from 5 days after seeding stages. Amount of sucrose in cotyledon of kidney seeds and seedlings increased continuously after germination. The amount of glucose and fructose in the cotyledons of kidney seeds during germination varied 5~10% or 5~15% but in the germinating and growing organs, plumule, they increased continuously after germination.

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