• 제목, 요약, 키워드: isoflavone

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Isoflavone 섭취가 흰쥐의 지방대사 및 면역능력에 미치는 영향 (The Effects of Isoflavone on Lipid Metabolism and Immune Responses in SD Rats)

  • 정미경;방명희;설소미;김우경
    • Journal of Nutrition and Health
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    • v.35 no.6
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    • pp.635-642
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    • 2002
  • This study was performed to investigate the effects of isoflavone consumption on plasma and liver lipid profiles and immune responses in Sprague-Dawley male rats. Experimental animals fed isoflavone at various doses for 4 weeks (0, 1095, 2190, 4380 isoflavone mg/kg diet). Exposure to isoflavone decreased the food consumption and final body weights of rats without decreasing the relative weights of organs, hemoglobin and hematocrit. And the plasma cholesterol and LDL-cholesterol, liver total lipid, cholesterol and triglyceride concentrations were significantly decreased by isoflavone intakes. The absolute and relative weights of thymus were significantly decreased in groups fed isoflavone than in control. Also splenocyte proliferations with Con A or PHA were decreased according to isoflavone consumption in rats, although there was not significant. These results demonstrate that isoflavone intakes significantly improve lipid profiles in plasma and liver. But the effects of isoflavone intakes on immune responses are needed further experiments.

산가수분해시 산의 농도가 탈지대두박의 isoflavone에 미치는 영향 (Effects of Acid Hydrolysis on Isoflavone of Defatted Soybean Flour)

  • 여경은;김우정
    • 한국식품과학회지
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    • v.34 no.5
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    • pp.916-918
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    • 2002
  • 산가수분해시 산의 농도가 탈지대두박의 isoflavone 양에 미치는 영향을 조사하였다. 측정한 isoflavone은 daidzin, genistin 등 glucosides와 daidzein, genistein 등 aglycons이었다. 산가수분해는 탈지대두박 분말에 0.25 N-3.00 N HCl을 가하고 $95^{\circ}C$에서 4시간 가열하였다. 그 결과 총 isoflavone은 0.25 N-1.0 N HCl의 범위에서 가열이 진행되면서 증가하여 2시간 후에는 약 40% 증가함을 보였다. 그러나 그 이상의 농도에서는 현저한 감소가 있어 4시간 후에는 52%의 감소가 있었다. 이 결과는 낮은 HCl 농도에서의 증가는 isoflavone isomer가 isoflavone으로 전환되었음을 보여주고 높은 농도에서의 감소는 isoflavone이 분해됨을 보여주고 있다. 가열 중 각각의 isoflavone의 변화는 전체적으로 glucosides는 감소하고 aglycons는 증가하는 경향이었다. 특히 2 N HCl에서의 60분 반응시 daidzin과 genistin, glycitin 등 glucosides는 검출되지 않았다.

Isoflavone Composition within Each Structural Part of Soybean Seeds and Sprouts

  • Phommalth, Siviengkhek;Jeong, Yeon-Shin;Kim, Yong-Hoon;Hwang, Young-Hyun
    • Journal of Crop Science and Biotechnology
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    • v.11 no.1
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    • pp.57-62
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    • 2008
  • Isoflavone content in various parts of six soybean cultivars and soybean sprout during germination was analyzed by high performance liquid chromatography. The parts analyzed were seed coat, cotyledon, and axis for seeds and whole sprout, root, hypocotyl, and cotyledon for sprout. Two cultivars, Aga3 which is known to have the smallest seed size and the highest isoflavone content among the Korean soybean cultivars and Pungsannamulkong which is the most widely being used as soy-sprout, were selected for sampling from 1 to 10 days after germination. At the same weight, the order of isoflavone content increased from seed coat to cotyledon to axis. The highest total isoflavone(isoflavone$\times$dry weight) content was observed in the cotyledon and the lowest in the seed coat. The cotyledon of the Aga3 variety had the highest total isoflavone content and the lowest was measured in the Pungsannamulkong variety. The highest total isoflavone content, $10,788{\mu}g/g$, was observed in whole sprouts(cotyledon+hypocotyl+root) on day 7 for Aga3. After day 7, there was a decreasing trend in isoflavone content as the germination period increased. Total isoflavone content in the cotyledon of Aga3 significantly increased after seed germination, whereas the isoflavone content in the cotyledon of Pungsannamulkong decreased. However, total isoflavone content in the root of both varieties increased while isoflavone content in the hypocotyls decreased after seed germination.

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Estimated dietary isoflavone intake among Korean adults

  • Lee, Min-June;Kim, Jung-Hyun
    • Nutrition Research and Practice
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    • v.1 no.3
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    • pp.206-211
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    • 2007
  • This study estimated the isoflavone intake level in Koreans using Food Frequency Questionnaire and analyzed related variables. The results showed that the average daily intake of isoflavone in adults was shown as 23.1 mg. The isoflavone intake level at 50 percentile was 16.9 mg ($0{\sim}90\;mg$), and 10% of adults took almost 50 mg of isoflavone a day and 10% took about 5 mg a day. The major food sources for isoflavone in Koreans were in the order of soybean, soybean paste, soy milk, soybean curd (tofu), and bean sprouts; the intake was different depending on age, educational background, occupation, economic standard, and family type. The result showed higher isoflavone intake levels in the group over 30 years old and the highest isoflavone intake in subjects working in farming/fishery, followed by housemakers. According to the differences by families the families with elderly members showed 50% higher isoflavone intake than young families with friends or siblings. Depending on related ecological variables, therefore, various nutrition education programs should be developed for a variety of intakes of soybean foods, along with easy and simple cooking methods as parts of continuous research.

파종기 이동에 따른 콩의 Isoflavone 함량 변화 (Isoflavone Contents of Soybean according to Different Planting Dates)

  • 윤홍태;김욱한;이영호;서세정;김시주
    • 한국작물학회지
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    • v.51 no.spc1
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    • pp.174-178
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    • 2006
  • 본실험은 조생종인 하대두형 및 중국수집종 콩과 중숙${\sim}$만숙종의 국내 육성 종과의 isoflavone 함량변이를 비교하며, 파종기 이동 및 콩의 생육단계별 생육기간에 따른 isoflavone함량의 차이를 분석하고자 수행하였으며, 결과를 요약하면 다음과 같다. 1. 전체 isoflavone함량은 파종기 이동에 관련 없이 국내 수집종이 하대두형 및 중국수집 종에 비하여 놓았으며, 하대 두형콩과 중국수집 종과 비슷한 함량 분포를 나타냈다. 2. 파종기이동에 따른 총 isoflavone 함량은 파종기를 늦게 할수록 하대두형, 중국수집종 및 국내육성종 모두에서 증가하는 경향 이었다. 3. 콩의 개화기${\sim}$성숙기의 기간 및 생육일수가 길어질수록 isoflavone 함량도 높아지는 경향을 나타냈으며, 통계적으로도 부합되었다. 4. 콩의 isoflavone 함량은 입중이 무거울수록 함량이 낮아 졌으며, 상관계수는 4월${\sim}$5월 파종구에서 각각 -0.57, -0.51 및 -0.38로 분석되었다.

재배 콩나물 저장 중 온도처리가 isoflavone 함량에 미치는 영향 (Effects of storage duration and temperature on the isoflavone content of full grown soy-sprouts)

  • 정연신;이향미;;김용훈;이정동;신동현;황영현
    • Current Research on Agriculture and Life Sciences
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    • v.26
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    • pp.55-62
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    • 2008
  • 콩나물로부터 고 isoflavone 함량의 콩 식품원료를 얻고자 콩나물 재배 시 빛을 쪼여주고, 재배한 콩나물의 저장온도를 달리한 조건에서 isoflavone 함량변이를 조사하였다. 공시 품종들의 7일 재배 후 저장하기 전 isoflavone 함량은 아가 3호>아가 1호>아가 2호>아가 4호>장기콩>보석콩>은하콩>풍산나물콩 순이었는데 아가 3호의 isoflavone 함량은 $4,619{\mu}g/g$이었다. 재배한 콩나물의 isoflavone 함량은 저장온도에 따라 품종별로 상이한 차이를 보였는데, 저장온도 $6^{\circ}C$$20^{\circ}C$에서 isoflavone 함량이 높은 반면 저장온도 $9^{\circ}C$$30^{\circ}C$에서는 대부분 콩 품종들의 isoflavone 함량은 낮았다. 저장일수에 따른 isoflavone의 함량은 품종들 간에 일정한 경향이 없었으나 대부분의 품종들은 저장기간 3일 이내에 가장 높은 isoflavone 함량을 보였고, 아가 4호와 장기콩은 저장기간 5일에서도 높은 isoflavone의 함량을 보였다. 저장기간과 온도처리를 하여 재배한 콩나물의 isoflavone 함량을 높이고자 한 결과 아가 3호를 포함하여 공시한 8품종 중 4품종에서 $20^{\circ}C$에서 하루 동안 저장했을 때 가장 높은 isoflavone의 함량을 나타내었다. 재배한 콩나물들 중 저장을 통해 가장 높은 isoflavone의 함량을 보인 품종은 아가 3호로써 저장후의 isoflavone 함량은 $11,705{\mu}g/g$이었다. 본 연구에서는 콩나물 저장기간 동안 수분공급이 지속적으로 이루어졌기 때문에 콩나물이 계속 성장한 것으로 생각된다. 따라서 저장기간 동안 온도처리를 달리하여 isoflavone의 함량을 증가시키는 보다 확실한 방법을 확립하기 위해서는 더 많은 연구가 이루어져야 할 것으로 생각되어진다.

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Microencapsulation of Water-Soluble Isoflavone and Physico-Chemical Property in Milk

  • Seok, Jin-Seok;Kim, Jae-Soon;Kwak, Hae-Soo
    • Archives of Pharmacal Research
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    • v.26 no.5
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    • pp.426-431
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    • 2003
  • This study was carried out to investigate the addition of water-soluble isoflavone into milk by means of microencapsulation technique. The yield of microencapsulation, sensory attributes, and capsule stability of water-soluble isoflavone microcapsules in milk were measured. Coating materials used was polyglycerol monostearate (PGMS), and core material was water-soluble isoflavone. The encapsulation yield of water-soluble isoflavone with PGMS was 67.2% when the ratio of coating material to core material was 15 : 1. The rate of water-soluble isoflavone release from capsules was 18, 19, and 25% when stored at 4,20, and $30^{\circ}C$ for 12 days in milk, respectively. In sensory evaluation, beany flavor and color of microencapsuled water-soluble isoflavone added milk were significantly different from uncapsuled water-soluble isoflavone added milk, however, bitterness was not significantly different. In vitro study, micro-capsules of water-soluble isoflavone in simulated gastric fluid with the range of 3 to 6 pHs were released 3.0∼15.0%, however, the capsules in simulated intestinal fluid with pH 7 were released 95.7% for 40 min incubation time. In conclusion, this study provided that PGMS as coating materials was suitable for the microencapsulation of water-soluble isoflavone, and the capsule containing milk was almost not affected with sensory attribute.

Application of Microencapsulated Isoflavone into Milk

  • Jeon, Byung-Ju;Kim, Nam-Chul;Han, Eun-Mi;Kwak, Hae-Soo
    • Archives of Pharmacal Research
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    • v.28 no.7
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    • pp.859-865
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    • 2005
  • This study was designed to develop a microencapsulated, water-soluble isoflavone for application into milk and to examine the hypocholesterolemic effect of such a milk product in a rat diet. The coating material was medium-chain triglyceride (MCT) and the core material was watersoluble isoflavone. The microencapsulation efficiency was 70.2% when the ratio (w/w) of coating material to core material was 15:1. The isoflavone release from the microcapsules was 8% after 3-day storage at $4^{\circ}C$. In in vitro study, 4.0-9.3% of water-soluble isoflavone in simulated gastric fluid was released in the pH range of 2 to 5 after 60 min incubation; however, in simulated intestinal fluid at pH 8, 87.6% of isoflavone was released from the capsules after 40 min incubation time. In sensory analysis, the scores of bitterness, astringency, and off-taste in the encapsulated isoflavone-added milk were slightly, but not significantly, different from those in uncapsulated, isoflavone-added milk. In blood analysis, total cholesterol was significantly decreased in the isoflavone-added group compared with that in the control after 6-week feeding. Therefore, this study confirmed the acceptability of MCT as a coating material in the microencapsulation of water-soluble isoflavone for application into milk, although a slight adverse effect was found in terms of sensory attributes. In addition, blood total cholesterol was lowered in rats which had been fed a cholesterol-reduced and microencapsulated, isoflavoneadded milk for 6 weeks.

Identification of Quantitative Trait Loci Associated with Isoflavone Contents in Soybean Seed

  • Kim Myung Sik;Park Min Jung;Hwang Jung Gyu;Jo Soo Ho;Ko Mi Suk;Chung Ill Min;Chung Jong Il
    • 한국작물학회지
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    • v.49 no.5
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    • pp.423-428
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    • 2004
  • Soybean seeds contain high amounts of isoflavones that display biological effects and isoflavone content of soybean seed can vary by year, environment, and genotype. Objective of this study was to identify quantitative trait loci that underlie isoflavone content in soybean seeds. The study involved 85 $F_2$ populations derived from Korean soybean cultivar 'Kwangkyo' and wild type soybean 'IT182305' for QTL analysis associated with isoflavone content. Isoflavone content of seeds was determined by HPLC. The genetic map of 33 linkage groups with 207 markers was constructed. The linkage map spanned 2,607.5 cM across all 33 linkage groups. The average linkage distance between pair of markers among all linkage groups was 12.6 cM in Kosambi map units. Isoflavone content in $F_2$ generations varied in a fashion that suggested a continuous, polygenic inheritance. Eleven markers (4 RAPD, 3 SSR, 4 AFLP) were significantly associated with isoflavone content. Only two markers, Satt419 and CTCGAG3 had F-tests that were significant at P<0.01 in $F_2$ generation for isoflavone content. Interval mapping using the $F_2$ data revealed only two putative QTLs for isoflavone content. The peak QTL region on linkage group 3, which was near OPAG03c, explained $14\%$ variation for isoflavone content. The peak QTL region on linkage group 5, which was located near OPN14 accounted for $35.3\%$ variation for isoflavone content. Using both Map-Maker-QTL $(LOD{\geq}2.0)$ and single-factor analysis $(P{\leq}0.05)$, one marker, CTCGAG3 in linkage group 3 was associated with QTLs for isoflavone content. This information would then be used in identification of QTLs for isoflavone content with precision

이소플라본 보충이 난소절제 흰쥐의 골대사에 미치는 영향 (The Effect of Isoflavone Supplementation on Bone Metabolism in Ovariectomized SD Rats)

  • 박영희;윤선;정수연;양승오;유태무;양지선;권대중
    • 한국식품영양과학회지
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    • v.30 no.4
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    • pp.657-661
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    • 2001
  • 이소플라본 보충이 골다공증의 예방 및 치료에 미치는 영향을 관찰하고자 Sprague-Dawley 암컷을 수술없이 일반식이를 제공한 군(CON), sham-operation 후 일반식이를 제공한 군(SH), 난소절제한 뒤 일반식이를 제공한 군(OVX), 난소절제 후 isoflavone을 공급한 군(OL, OM, OH)으로 나눈 뒤, 난소절제 4일 및 8주 후부터 8주간 이소플라본을 공급하여 혈액의 ALP, sy 중의 hydroxyproline의 변화, 골밀도, 골강도 및 골무게 등을 연구하였다. 골다공증의 예방에 대하여 isoflavone의 효과를 관찰한 결과, ALP는 난소를 절제한 OVX군에서는 CONrns과 SH군에 비해 유의적으로 높은 값을 보였다. 그러나, 난소절제 후 4일부터 8주간 isoflavone을 보충시킨 결과 ALP 활성과 urinary hydroxyproline 함량은 SH군과 비슷하게 낮아졌다. 특히 OM(0.8mg/kg diet)군의 경우 ALP활성과 urnary hydroxyproline 함량이 가장 낮아서 OVX군과 유의적인 차이를 보였다.(p<0.05). 난소적출 후 8주 후에 골밀도를 측정한 결과 난소절제군의 골밀도가 CONrns과 SH군에 비해 유의적으로 낮은 것으로 나타났다. Isoflavone을 보충한 결과 골밀도가 증가하였으나 유의적인 차이는 없어다. 골다공증의 치료효과에 대한 연구결과 alkaline phosphatase activity의 감소, 골밀도, 골강도 및 골무게가 증가하였으나 유의적인 차이는 나타나지 않았다. 이상의 결과는 난소절제 후에 일어나는 골격 대사의 이상은 isoflavone을 보충시킴으로써 어느 정도 정상화시킬 수 있음을 시사한다. 따라서 적절한 양의 isoflavone 보충은 폐경 후 여성의 골대사에 유익한 효과를 줄 것으로 풀이된다.

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