• Title, Summary, Keyword: immobilization stress

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Effects of Chungganhaewooltang on Serum Levels of Histamine and Corticosterone and Immune Response after Immobilization-Stress or Cold-Stress in Mice (청간해울탕(淸肝解鬱湯)이 생취에 Immobilization-Stress 및 Cold-Stress 부하후(負荷後) 혈중(血中)Histamine과 Corticosterone 함량(含量) 및 면역능(免疫能)에 미치는 영향(影響))

  • Kang, Bok-Hwan;Jung, Woo-Suk;Kim, Song-Baeg;Yoo, Sim-Keun
    • The Journal of Korean Obstetrics and Gynecology
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    • v.18 no.4
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    • pp.85-105
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    • 2005
  • Purpose : Investigate the effects of Chungganhaewooltang(CHT) on immobilization-stress or cold-stress in C576BL/6J mice. Methods : Male C57BL/6J 30 mice of weighting 18${\pm}$2g, were divided into sixs groups including the immobilization-stress group(5heads), after immobilization-stress CHT oral administration(500mg/kg) groups(5heads), cold-stress group(5heads) and after cold-stress CHT oral administration(500mg/kg) groups(5heads). then we observed changes in the serum histamine and corticosterone level and changes immune system Results : Immobilization-stress or cold-stress increased the serum level of histamine and corticosterone. CHT decreased the serum level of histamine and corticosterone increased by cold-stress. CHT inhibited the release of histamine from mast cells at the concentration of 0.1 mg/ml. In addition, immobilization-stress or cold-stress decreased the cell viability of murine thymocytes and splenocytes. CHT increased the cell viability of thymocytes decreased by immobilization-stress or cold-stress, but did not affect the cell viability of splenocytes decreased by immobilization-stress or cold-stress. Also immobilization-stress or cold-stress increased DNA fragmentation of thymocytes and splenocytes. CHT decreased DNA fragmentation of thymocytes increased by immobilization-stress or cold-stress, but did not affect DNA fragmentation of splenocytes increased by immobilization-stress or cold-stress. Immobilization-stress increased the population of thymic $CD4^+$ cells. CHT decreased the population of thymic $CD4^+$ cells increased by immobolization-stress. Immobilization-stress or cold-stress decreased the population of $B220^+$ cells and increased the population of $thy1^+$ cells. CHT decreased the population of $thy1^+$ cells increased by immobilization-stress or cold-stress. Immobilization-stress or cold-stress increased the population of splenic $CD4^+$ cells and $CD8^+$ cells. CHT decreased the population of splenic $CD4^+$ cells increased by immobolization-stress or cold-stress. Immobilization-stress or cold-stress decreased the production of ${\gamma}-interferon$(IFN) interleukin(IL)-2 and IL-4. CHT enhanced the production of ${\gamma}-IFN$ decreased by immobilization-stress or cold-stress but did not affect the production of IL-2 and IL-4 decreased by immobilization-stress or cold-stress. Furthermore, Immobilization- stress or cold-stress decreased the phagocytic activity of peritoneal macrophages and the production of nitric oxide. CHT enhanced the phagocytic activity and nitric oxide production decreased by cold-stress. Conclusion : CHT may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of immune response.

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Effects of Haeganjeon on Immobilization-Stress or Cold-Stress in Mice (해간전(解肝煎)이 생쥐의 Immobilization-Stress 및 Cold-Stress에 미치는 영향(影響))

  • Hwang, Tae-Won;Sim, Ho-Cherl;Kim, Song-Baeg;Yoo, Sim-Keun;Cho, Han-Back
    • The Journal of Korean Obstetrics and Gynecology
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    • v.18 no.3
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    • pp.51-66
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    • 2005
  • Purpose : Investigate the effects of Haeganjeon water extract (HGJ) on immobilization-stress or cold-stress in C57BL/6J mice. Methods : Male C57BL/6J 30 mice of weighting 18${\pm}$2g, were divided into sixs groups including the immobilization-stress group(5heads), after immobilization-stress HGJ oral administration(500mg/kg) groups(5heads), cold-stress group(5heads) and after cold-stress HGJ oral administration(500mg/kg) groups(5heads). then we observed changes in the serum histamine and corticosterone level and changes immune system. Results : HGJ decreased the serum level of histamine and corticosterone increased by immobilization-stress or cold-stress. HGJ inhibited the release of histamine from mast cells. In addition, HGJ enhanced the cell viability of thymocytes decreased by immobilization-stress or cold-stress and decreased DNA fragmentation of thymocytes increased by immobilization- stress or cold-stress. Also, HGJ increased the cell viability of splenocytes decreased by cold-stress and decreased DNA fragmentation of splenocytes increased by cold-stress. HGJ decreased the population of thymic CD4+ cells increased by immobolization-stress. HGJ increased the population of B220+ cells decreased by immobilization-stress and decreased the population of Thy1+ cells increased by immobilization-stress. Also, HGJ decreased the population of splenic CD4+ cells increased by immobolization-stress. HGJ enhanced the production of ${\gamma}-interferon$ decreased by immobilization-stress or cold-stress and increased the production of interleukin-4 decreased by immobilization-stress. Furthermore, HGJ enhanced the phagocytic activity decreased by immobilization-stress or cold-stress and enhanced the level of nitric oxide decreased by immobilization-stress or cold-stress. Conclusion : HGJ may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of immune response.

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Effects of Kamikwibitang on Serum Levels of Corticosterone and Histamine and Immune Response after Immobilization Stress in Mice (가미귀비탕(加味歸脾湯)이 생쥐에 Immobilization Stress 부하후(負荷後) 혈중(血中) Corticosterone과 Histamine 함량(含量) 및 면역능(免疫能) 변화(變化)에 미치는 영향(影響))

  • Wi, Seok;Lee, Seung-Jun;Yoo, Sim-Keun
    • The Journal of Korean Obstetrics and Gynecology
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    • v.18 no.1
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    • pp.94-110
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    • 2005
  • The purpose of this research was to investigate the effects of Kamikwibitang water extract (KKT) on immobilization stress in C57BL/6J mice. KKT decreased the serum level of histamine and corticosterone increased by immobilization stress. In addition, KKT decreased the cell viability of thymocytes and enhanced the cell viability of splenocytes decreased by immobilization stress. Also, KKT decreased the viability of thymocytes and splenocytes in vitro. KKT decreased DNA fragmentation of splenocytes increased by immobilization stress. KKT decreased the population of thymic $CD4^+CD8^-$ cells increased by immobilization stress, and did not affect the population of $B220^+$ cells and the population of $Thy1^+$ cells changed by immobilization stress and enhanced the population of splenic $CD4^-CD8^+$ cells increased by immobilization stress. KKT enhanced the production of ${\gamma}-interferon$ and did not affect the production of interleukin-2 and interleukin-4 decreased by immobilization stress. Also, KKT decreased the phagocytic activity and the level of nitric oxide decreased by immobilization stress. These results indicate that KKT may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of specific-immune response.

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Effects of Hwaganjeon on Immobilization-Stress or Cold-Stress in Mice (화간전(化肝煎)이 생쥐의 Immobilization-Stress 및 Cold-Stress에 미치는 영향(影響))

  • Park, Jong-Moon;Ko, Jeong-Min;Ahn, Kyu-Hwan;Choe, Chang-Min;Yoo, Sim-Keun
    • The Journal of Korean Obstetrics and Gynecology
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    • v.19 no.4
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    • pp.93-116
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    • 2006
  • Purpose : This study is to examine the effects of Hwaganjeon water extract(HGJ) on immobilization-stress or cold-stress in BALB/C mice. Methods : We have Hwaganjeon water extract(HGJ) by freeze-dryer & melt it by a saline solution. We feed HGJ 500mg/Kg to 5mice, and add immobilization-stress by putting mice in plastic cylinder 10 hours, and add cold-stress by putting mice in $4^{\circ}C$ cold room 6 hours. Results : 1. HGJ decreased the serum level of histamine and corticosterone increased by immobilization-stress or cold-stress. HGJ inhibited the release of histamine from mast cells at the concentration of 1 mg/ml. 2. HGJ did not affect the cell viability of thymocytes decreased by immobilization-stress or cold-stress, but increased the cell viability of splenocytes decreased by immobilization-stress or cold-stress. 3. HGJ decreased the population of splenic $CD4^{+}$ and $CD8^{+}$cells increased by immobolization-stress or cold-stress. 4. HGJ enhanced the production of ${\gamma}$-interferon(IFN) and interleukin(IL)-2 decreased by immobilization-stress or cold-stress. Conclusion : These results indicate that HGJ may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of immune response.

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Anti-Stress Effects of Ginseng in Immobilization-Stressed Rats

  • Choi, Eun-Ha;Lee, Hyun-Jung;Kim, Cheol-Jin;Kim, Jong-Tae;Kwun, In-Sook;Kim, Yang-Ha
    • Preventive Nutrition and Food Science
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    • v.9 no.3
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    • pp.253-258
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    • 2004
  • Stress is a global menace exacerbated by the advancement of industrialization. Failure of stress management is to a breakdown of the psychological and physiological protection mechanisms against stress. The aim of present study was to investigate the anti-stress potential of ginseng against immobilization stress. Male Sprague-Dawley rats (n=24) were divided into three groups; (i) control, (ii) immobilization stress (2hr daily, for 2 weeks), and (iii) immobilization stress (2 hr daily, for 2 weeks) plus oral administration of ginseng (200 mg/kg BW Id). Immobilization stress resulted in a significant inhibition of body weight gain by 45 % and a significant decrease in the tissue weights of thymus and spleen (p < 0.05). The concentrations of blood GOT and GPT were significantly increased in the immobilization-stressed group compared to the control group (p < 0.05). There were no differences in the blood cholesterol levels among groups. Ginseng administration in the immobilization-stressed group tended to reverse the lack of body weight gain and food intake, though not significantly. The ginseng-administered group showed a significant reversal in the stress-induced effect on spleen and thymus weight, increasing the tissue weights by 16% and 20%, respectively, compared to immobilization-stressed group (p<0.05). The plasma corticosterone level was significantly increased in the stressed group by 39 % compared to the control group (p<0.05), but ginseng administration significantly reversed the stress-induced increase in plasma corticosterone by 15 % compared to the immobilization-stressed group. The present study suggests that the anti-stress effect of ginseng is mediated by normalization of stress-induced changes in the circulating hormones and a reversal of tissue weight loss, thereby returning the body to normal homeostasis.

Effects of Kwibitang on the Specific Immune Response after Immobilization Stress in C57BL/6 Mice (귀비탕이 C57BL/6 Mouse에 Stress 부하 후 특이적 면역반응에 미치는 영향)

  • Lee Taek Yul;Han Mi Sook;Oh Chan Ho;Eun Jae Soon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.5
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    • pp.1208-1216
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    • 2003
  • Stress is known to influence the immune function via an effect on the central nervous system. To investigated the effects of Kwibitang water extract (KBT) on the specific immune response in C57BL/6 mice stressed by immobilization, we evaluated the changes in the cell viability, DNA fragmentation and subpopulation of thymocytes and splenocytes. KBT enhanced the cell viability of thymocytes and splenocytes decreased by immobilization stress. Also, KBT decreased DNA fragmentation of thymocytes and splenocytes increased by immobilization stress. KBT decreased the population of CD4/sup +/ cells and CD8/sup +/ cells in thymocytes and Thy1/sup +/ cells in splenocytes increased by immobolization stress, but increased the population of B220/sup +/ cells decreased by immobilization stress. In addition, KBT enhanced the production of ν-interferon and IL-2 decreased by immobilization stress. These results indicate that KBT may be useful for the prevention and treatment of stress via enhancement of the specific immune response

Regulation of Endoplasmic Reticulum Stress Response by the Immobilization Stress (부동스트레스에 의한 소포체스트레스반응 조절)

  • Kwon, Ki-Sang;Kwon, Young-Sook;Kim, Seung-Whan;Kim, Dong-Woon;Kwon, O-Yu
    • Journal of Life Science
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    • v.22 no.8
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    • pp.1132-1136
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    • 2012
  • Many kind of cell stresses induce gene expression of unfolded protein response (UPR)-associated factors. This study demonstrated that up- and down-regulation of gene expression of endoplasmic reticulum (ER) stress chaperones and ER stress sensors was induced by immobilization stress in the rat organs (adrenal gland, liver, lung, muscle). However, no statistically significant regulation was detected in the others (heart, spleen, thymus, kidney, testis). The results are the first to show that immobilization stress induces UPR associated gene expression, will help to explain immobilization stress-associated ER stress.

Effects of Glycyrrhizae Radix on Serum Corticosterone and Blood Histamine Content by Immobilization Stress in Mice (감초(甘草) 엑기스가 Immobilization Stress 부하(負荷)후 혈중(血中) Corticosterone 및 Histamine 함량변화(含量變化)에 미치는 영향)

  • Eun, Jae-Soon;Oh, Chan-Ho;Han, Jong-Hyun
    • Korean Journal of Pharmacognosy
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    • v.20 no.1
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    • pp.37-42
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    • 1989
  • This study was carried out to investigate the effect of Glycyrrhizae Radix on serum corticosterone and blood histamine content by immobilization stress in mice. Corticosterone secretion and blood histamine level was significantly increased in mice by subjecting the animals to immobilization stress 1 hr. after intraperitoneal injection of Glycyrrhizae Radix extract (150 mg/kg) and glycyrrhizinic and (15 mg/kg). whereas, administration of cortisol $(7.5\;{\mu}g/kg)$ provoked a decrease in corticosterone secretion and histamine levels. These results suggested that glycyrrhizinic acid was effective on cortiosterone release provoked by immobilization stress and this release was mediated in part by histamine.

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Effect of Sulfonylureas Administered Centrally on the Blood Glucose Level in Immobilization Stress Model

  • Sharma, Naveen;Sim, Yun-Beom;Park, Soo-Hyun;Lim, Su-Min;Kim, Sung-Su;Jung, Jun-Sub;Hong, Jae-Seung;Suh, Hong-Won
    • The Korean Journal of Physiology and Pharmacology
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    • v.19 no.3
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    • pp.197-202
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    • 2015
  • Sulfonylureas are widely used as an antidiabetic drug. In the present study, the effects of sulfonylurea administered supraspinally on immobilization stress-induced blood glucose level were studied in ICR mice. Mice were once enforced into immobilization stress for 30 min and returned to the cage. The blood glucose level was measured 30, 60, and 120 min after immobilization stress initiation. We found that intracerebroventricular (i.c.v.) injection with $30{\mu}g$ of glyburide, glipizide, glimepiride or tolazamide attenuated the increased blood glucose level induced by immobilization stress. Immobilization stress causes an elevation of the blood corticosterone and insulin levels. Sulfonylureas pretreated i.c.v. caused a further elevation of the blood corticosterone level when mice were forced into the stress. In addition, sulfonylureas pretreated i.c.v. alone caused an elevation of the plasma insulin level. Furthermore, immobilization stress-induced insulin level was reduced by i.c.v. pretreated sulfonylureas. Our results suggest that lowering effect of sulfonylureas administered supraspinally against immobilization stress-induced increase of the blood glucose level appears to be primarily mediated via elevation of the plasma insulin level.

Anti-stress and Anti-nociceptive Effects of the Aqueous Extracts of Chungsimyeonja-eum against Immobilization Stress and Incision Pain in Rats (청심포자음(淸心蒲子飮)의 구속 스트레스 및 절개 통증에 대한 항스트레스 작용과 진통효과)

  • Yoon, Jeong-Hwan;Ko, Il-Gyu;Shin, Mal-Soon;Kim, Chang-Ju;Lee, Chung-Yeol
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.4
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    • pp.874-883
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    • 2007
  • The aqueous extract of Chungsimyeonja-eum has been used to treat palpitation, anemia, chronic fatigue, hypertension, and stroke. It also possesses various pharmacological effects including hypotensive, blood circulating, sedative, anti-inflammatory, diuretic, and anti-stress activities. In the present results, the latency time of plantar test was decreased in the immobilization stress and incusion pain-induction group. However the paw withdrawal latency values were increased in the immobilization stress and incision pain-induction groups after treatment with the aqueous extract of Chungsimyeonja-eum. The c-Fos expression in the paraventricular nucleus (PVN), and 5-hydroxytryptamine (5-HT) and tryptophan hydroxylase (TPH) in the dorsal raphe were enhanced in the immobilization stress and incision pain-induction groups. However, the treatment with the aqueous extract of Chungsimyeonja-eum suppressed the immobilization stress and incision pain-induced increase of c-Fos, 5-HT, and TPH expressions. Here in this study, we have demonstrated the protective effects of Chungsimyeonja-eum on immobilization and incision pain-induced stress. The present study revealed that Chungsimyeonja-eum treatment diminishes immobilization and pain stress.