• Title, Summary, Keyword: histopathological examination

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TMA-OM(Tissue Microarray Object Model)과 주요 유전체 정보 통합

  • Kim Ju-Han
    • Proceedings of the Korean Society for Bioinformatics Conference
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    • pp.30-36
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    • 2006
  • Tissue microarray (TMA) is an array-based technology allowing the examination of hundreds of tissue samples on a single slide. To handle, exchange, and disseminate TMA data, we need standard representations of the methods used, of the data generated, and of the clinical and histopathological information related to TMA data analysis. This study aims to create a comprehensive data model with flexibility that supports diverse experimental designs and with expressivity and extensibility that enables an adequate and comprehensive description of new clinical and histopathological data elements. We designed a Tissue Microarray Object Model (TMA-OM). Both the Array Information and the Experimental Procedure models are created by referring to Microarray Gene Expression Object Model, Minimum Information Specification For In Situ Hybridization and Immunohistochemistry Experiments (MISFISHIE), and the TMA Data Exchange Specifications (TMA DES). The Clinical and Histopathological Information model is created by using CAP Cancer Protocols and National Cancer Institute Common Data Elements (NCI CDEs). MGED Ontology, UMLS and the terms extracted from CAP Cancer Protocols and NCI CDEs are used to create a controlled vocabulary for unambiguous annotation. We implemented a web-based application for TMA-OM, supporting data export in XML format conforming to the TMA DES or the DTD derived from TMA-OM. TMA-OM provides a comprehensive data model for storage, analysis and exchange of TMA data and facilitates model-level integration of other biological models.

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Molecular and Histopathological Evidence of Mycobacteriosis in Paradise Fish Macropodus opercularis Imported into Korea

  • Han, Hyun-Ja;Kim, Jeong-Ho;Jeon, Chan-Hyeok;Kim, Wi-Sik;Kim, Do-Hyung;Jung, Sung-Ju;Oh, Myung-Joo
    • Fisheries and aquatic sciences
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    • v.16 no.3
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    • pp.165-169
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    • 2013
  • We report on mycobacteriosis in an imported tropical ornamental fish Macropodus opercularis commonly known as the paradise fish. Mass mortality occurred in paradise fish imported to Korea from Southeast Asia in 2008. The affected fish did not show any outward clinical signs, but enlargement of the spleen, kidneys, and liver was observed on dissection. Histopathological examination revealed numerous granulomas in the spleen, and acid-fast bacilli were observed in the centers of the granulomas. About 65% of spleen DNA samples were PCR positive using mycobacteria-specific primers targeting the 16S rRNA and hsp65 genes. The nucleotide identities of the 16S rRNA and hsp65 genes with those of Mycobacterium marinum were 99.5% and 99.4%, respectively. Although the bacterium was not cultured, the molecular diagnosis and histopathological findings were consistent with mycobacteriosis in paradise fish.

Multiple fibromyxomas of the jaws: A case report

  • Zayet, Mohamed Khalifa;Eiid, Salma Belal
    • Imaging Science in Dentistry
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    • v.44 no.3
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    • pp.237-241
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    • 2014
  • Fibromyxoma of the jaw is a rare benign mesenchymal odontogenic tumor with locally aggressive behavior. In the present report, a 13-year-old female patient presented to our university hospital with delayed eruption of some of her teeth. A panoramic radiograph taken at the initial examination revealed four pericoronal radiolucencies related to the four third molars. Thereafter, a magnetic resonance imaging (MRI) examination was performed. After the surgical removal of these molars, the microscopic examination diagnosed the four lesions as fibromyxomas. Here, we have discussed the clinical, panoramic radiography, MRI, and histopathological findings of the case.

A CASE REPORT OF BRANCHIAL CLEFT CYST IN THE NECK (경부에 발생한 새열낭 (branchial cleft cyst)의 증례보고)

  • Lee Young-Mi;Choi Soon-Chul;Park Tae-Won;You Dong-Soo
    • The Journal of Korean Academy of Maxillofacial Radiology
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    • v.23 no.2
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    • pp.365-372
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    • 1993
  • Branchial cleft cyst is the most common lateral neck cyst; the vast majority are of the second branchial cleft origin. This presumably reflects the greater depth and longer persistence of the second cleft, compared with the first, third, and fourth clefts. We experienced a 49-year-old male whose chief complaint was a abnormal mass of the left parotid gland area and neck. As a result of careful analysis of clinical, radiological, and histopathological findings, we diagnosed it as a second branchial cleft cyst in the neck and obtained results as follows: 1. In clinical examination, there was a 10×15㎝ sized, fluctuant painful mass in the left neck and parotid area. 2. In radiographic examination, a low echogenic mass with internal cystic change in the inferior parotid gland area was noted sonographically. Computed tomograph showed a 3×4㎝ sized, well-defined cystic mass with heterogenous solid component in the anterior border of sternocleidomastoid muscle. MRI revealed 5×6㎝ sized, well-marginated multi separated mass in the same area. 3. In histopathological examination, lining of cyst was stratified squamous epithelium with typical lymph node pattern and inflammatory cell infiltration.

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CEMENTIFYING FIBROMA IN BOTH SIDES OF THE MANDIBLE (하악골 양측에 발생된 백악질섬유종)

  • Park Mi-Kyung;Choi Karp-Shik
    • The Journal of Korean Academy of Maxillofacial Radiology
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    • v.22 no.2
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    • pp.367-373
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    • 1992
  • The author observed a 35-year-old male patient who came to the Dental Infirmary of Kyungpook National University Hospital who had complained of gradual swelling on both side of the mandible for 15 years. As a result of careful analysis of clinical, radiological and histopathological findings, the authors diagnosed it as cementifying fibroma and obtained the results as follows: 1. In clinical examination, main clinical symptom was facial deformity due to gradual swelling on both side of the mandible. 2. In radiographic examination, radiolucent lesions with central radiopaque foci were seen on both mandibular body areas bilaterally, and cortical thinning and expansion of the mandibular body were seen buccolingually. And loss of lamina dura and root resortption of adjacent teeth were also seen. In histopathological examination, this lesion was composed of delicate interlacing collagen fibers interspersed by the fibroblasts and cementoblasts. And round or oval shaped basophilic masses of cementum-like tissue were observed in the connective tissue.

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Single and 4-Week Repeated Dose Toxicity Studies of DA-3585, a Recombinant Human Erythropoietin, in Rabbits (사람 적혈구 조혈인자 DA-3585의 토끼에 대한 단회 및 4주 반복투여 독성시험)

  • Cho, Hyeon;Kim, Dong-Hwan;Kang, Kyung-Koo;Baik, Nam-Gi;Kim, Won-Bae
    • Biomolecules & Therapeutics
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    • v.6 no.2
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    • pp.171-181
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    • 1998
  • DA-3585, a biosynthetic recombinant human erythropoietin has been developed as a treatment for anemia associated with chronic renal failure in Dong-A pharmaceutical Co. Ltd. This study was carried out to assess its acute and subacute toxicities in rabbits. DA-3585 was intravenously administered to rabbits at dose levels of 6250, 12500 or 25000 lU/kg for single dose toxicity study and at dose levels of 100, 500 or 2500 lU/kg/day for 4-week repeated dose toxicity study. In the acute toxicity study, dose up to 25000 lU/kg had no adverse effect on the behavior or body weight gain. Pathological examinations revealed no abnormal gross lesions related to DA-3585. In the subacute toxicity study, all animals survived until termination of treatment. DA-3585 had no influence on clinical signs, food and water intake or on body weight changes. Hematological examination showed increases in the number of RBC, hemoglobin contents and hematocrit values with a dose dependent manner in the animals treated with DA-3585. Histopathological examination revealed erythroid hyperplasia in the bone marrow and extramedullary hematopoiesis in the liver. The changes detected in the hematological and histopathological examination presumably represent exaggerated pharmacological effects of erythropoietin. The NOAEL (no-observed-adverse-effect-level) of DA-3585 was estimated to be 100 lU/kg/ day under this study condition.

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Primary Salivary Gland Adenocarcinoma in a Dog

  • An, Su-Jin;Kim, Dae-Young;Ahn, Soo-Min;Jung, Dong-In;Hwang, Tae-Sung;Lee, Hee-Chun;Lee, Jae-Hoon;Yu, Do-Hyeon
    • Journal of Veterinary Clinics
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    • v.35 no.6
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    • pp.308-310
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    • 2018
  • A 17-year-old neutered male Miniature Pinscher dog presented with a mass on the left side of the submandibular region. Fine needle aspiration revealed malignant epithelial cells from the salivary gland but no evidence of metastasis was found on radiography, ultrasonography, or computed tomography. The cervical mass was surgically resected, and the histopathological examination confirmed adenocarcinoma of the mandibular salivary gland. Seven months after the initial diagnosis, the dog is alive without any clinical signs. This report describes the clinical findings, cytology, diagnostic imaging, and histopathological characteristics of a mandibular salivary gland adenocarcinoma in a Miniature Pinscher dog.

Clinical and Ultrasonographic Studies for the Liver Lesion Induced by Tetrachlorethylene in Dogs (개에서 Tetrachlorethylene에 의해 유발된 간장 병변의 임상 및 초음파학적 연구)

  • 김영범;김명철
    • Journal of Veterinary Clinics
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    • v.16 no.2
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    • pp.321-327
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    • 1999
  • This study was performed to examine the clinical, serological, ultrasonographic and pathological findings in dogs with acute liver disease induced by tetrachlorethylene at 4 times of anthelminthic oral dose. The results obtained through this experiment could be summarized as follows: 1. The dogs administered with tetrachlorethylene, revealed decreased body weight, and showed lethargy and depression. 2. In serological findings, bilirubin values slightly increased, AST and ALT was decreased at 1∼3 days, and after that time increased according to the lapse of days, and revealed the highest at 5 days, and decreased to normal values at 6 days. 3. In ultrasonographic findings, branches of the portal vein were increased, the echodensity of the liver parenchyma was decreased at early stage, and increased at mid stage, and decreased at last stage. 4. In histopathological findings, necrosis of parenchymal cell, and perivascular hemorrhage were observed more severely at 6 days, as compared with 3 days. There results suggest that ultrasonographic examination is considered to be a more simple, rapid, non-invasive and useful diagnostic method for acute liver parenchymal lesion.

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Malignant Anal Sac Melanoma in a Dog

  • Ahn, Soo-Min;Cho, Kyoung-Oh;Kim, Sang-Ki;Jung, Dong-In;Yu, DoHyeon
    • Journal of Veterinary Clinics
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    • v.36 no.6
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    • pp.349-352
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    • 2019
  • A five-year-old female Yorkshire Terrier dog presented with a perianal mass. Fine needle aspiration revealed that the mass comprised two different types of cells: hepatoid epithelial cells and melanin-containing melanocytes. Histopathological examination confirmed perianal gland adenoma with malignant melanoma. Evidence of metastasis was found on thoracic radiography with soft-tissue densities observed within the pulmonary parenchyma. The dog survived for three months after diagnosis of malignant melanoma. This report describes the clinical findings, diagnostics used, cytological and histopathological findings, and the potential prognosis for a dog diagnosed with malignant anal sac melanoma.

A Comparison Study of Magnetic Resonance Imaging Findings and Neurological Signs in Canine Brain Diseases

  • Kim, Min-Ju;Song, Joong-Hyun;Hwang, Tae-Sung;Lee, Hee-Chun;Yu, Do-Hyeon;Kang, Byeong-Teck;Jung, Dong-In
    • Journal of Veterinary Clinics
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    • v.35 no.5
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    • pp.178-183
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    • 2018
  • The object of this study was to compare magnetic resonance imaging (MRI) findings and neurological signs in canine brain diseases. Brain diseases can cause severe neurological deficits and may be life-threatening. The antemortem diagnosis of the brain diseases is difficult for the clinician, since definitive diagnosis is based upon histopathological confirmation. Brain diseases are often associated with specific clinical signs, signalment, progression, and location. Accurate lesion localization through neurological examination and MRI findings is helpful for developing a differential diagnosis. A retrospective study was performed to compare the neurological examination of dogs with suspected brain disease to the MRI findings. Based on this study, neurological examination is a reliable way to localize most brain lesions. Postural reaction deficits do not provide sufficient information to localize lesions. Additionally, not all brain lesions present clinical signs and inflammatory lesions may cause no detectable abnormalities on MRI. Therefore, in clinical practice, a combination of neurological examination and MRI findings recommended for accurate brain lesion localization.