• Title, Summary, Keyword: histopathological examination

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Effect of Methotrexate on Collagen-Induced Arthritis Assessed by Micro-Computed Tomography and Histopathological Examination in Female Rats

  • Kim, Young Hee;Kang, Jin Seok
    • Biomolecules & Therapeutics
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    • v.23 no.2
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    • pp.195-200
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    • 2015
  • We tested the hypothesis that micro-computed tomography (micro-CT) analysis provides a better quantitative readout of the therapeutic potential of methotrexate (MTX) for treating collagen-induced arthritis (CIA) in rats and compared to conventional histopathological examination. Rats were divided into three groups: Group 1 (G1) was treated with 0.9% saline, whereas groups 2 (G2) and 3 (G3) were boosted with type II collagen at days 0 and 7. Following the first collagen immunization, rats in G1 and G2 were treated with 0.9% saline and those in G3 were treated with 1.5 mg/kg MTX from day 14 to 28. All rats were sacrificed on day 28, at which point and all hind knee joints were analyzed by micro-CT and histopathological examination. Micro-CT analyses showed that bone volume and trabecular number were significantly decreased in G2 and G3 compared to G1 (p<0.01), as was percent bone volume (p<0.05 and p<0.01, respectively). However, bone surface/bone volume was significantly increased in G2 and G3 compared to G1 (p<0.05 and p<0.01, respectively). Trabecular separation was significantly increased in G3 compared to G1 (p<0.05). Histopathological examination showed that knee joints of rats in G2 and G3 showed severe joint destruction with inflammatory cell infiltration. However, cartilage destruction was slightly reduced in G3 compared to G2. Taken together, these results suggest that MTX treatment reduced cartilage destruction in rats with CIA, and micro-CT analyses made it possible to quantify arthritic bony lesion.

Efficacy of Bacteriophage Treatment in Murine Burn Wound Infection Induced by Klebsiella pneumoniae

  • Kumari, Seema;Harjai, Kusum;Chhibber, Sanjay
    • Journal of Microbiology and Biotechnology
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    • v.19 no.6
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    • pp.622-628
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    • 2009
  • In the present study, the therapeutic potential of purified and well-characterized bacteriophages was evaluated in thermally injured mice infected with Klebsiella pneumoniae B5055. The efficacy of five Klebsiella phages (Kpn5, Kpn12, Kpn13, Kpn17, and Kpn22) was evaluated on the basis of survival rate, decrease in bacterial counts in different organs of phage-treated animals, and regeneration of skin cells as observed by histopathological examination of phage-treated skin. Toxicity studies performed with all the phages showed them to be non-toxic, as no signs of morbidity and mortality were observed in phage-treated mice. The results of the study indicate that a single dose of phages, intraperitoneally (i.p.) at an MOI of 1.0, resulted in significant decrease in mortality, and this dose was found to be sufficient to completely cure K. pneumoniae infection in the burn wound model. Maximum decrease in bacterial counts in different organs was observed at 72 h post infection. Histopathological examination of skin of phage-treated mice showed complete recovery of burn infection. Kpn5 phage was found to be highly effective among all the phages and equally effective when compared with a cocktail of all the phages. From these results, it can be concluded that phage therapy may have the potential to be used as stand-alone therapy for K. pneumoniae induced burn wound infection, especially in situations where multiple antibiotic-resistant organisms are encountered.

Fluorescence Detection of Cell Death in Liver of Mice Treated with Thioacetamide

  • Kang, Jin Seok
    • Toxicological Research
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    • v.34 no.1
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    • pp.1-6
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    • 2018
  • The purpose of this study was to detect cell death in the liver of mice treated with thioacetamide (TAA) using fluorescence bioimaging and compare this outcome with that using conventional histopathological examination. At 6 weeks of age, 24 mice were randomly divided into three groups: group 1 (G1), control group; group 2 (G2), fluorescence probe control group; group 3 (G3), TAA-treated group. G3 mice were treated with TAA. Twenty-two hours after TAA treatment, G2 and G3 mice were treated with Annexin-Vivo 750. Fluorescence in vivo bioimaging was performed by fluorescence molecular tomography at two hours after Annexin-Vivo 750 treatment, and fluorescence ex vivo bioimaging of the liver was performed. Liver damage was validated by histopathological examination. In vivo bioimaging showed that the fluorescence intensity was increased in the right upper part of G3 mice compared with that in G2 mice, whereas G1 mice showed no signal. Additionally ex vivo bioimaging showed that the fluorescence intensity was significantly increased in the livers of G3 mice compared with those in G1 or G2 mice (p < 0.05). Histopathological examination of the liver showed no cell death in G1 and G2 mice. However, in G3 mice, there was destruction of hepatocytes and increased cell death. Terminal deoxynucleotidyl transferase dUTP nick end labeling staining confirmed many cell death features in the liver of G3 mice, whereas no pathological findings were observed in the liver of G1 and G2 mice. Taken together, fluorescence bioimaging in this study showed the detection of cell death and made it possible to quantify the level of cell death in male mice. The outcome was correlated with conventional biomedical examination. As it was difficult to differentiate histological location by fluorescent bioimaging, it is necessary to develop specific fluorescent dyes for monitoring hepatic disease progression and to exploit new bioimaging techniques without dye-labeling.

Squamous Cell Carcinoma in the Base of the Tongue in a Dog (개의 혀 뿌리에서 발생한 편평세포암종)

  • Kim, Se-Eun;Kim, Seung-Hyun;Shim, Kyung-Mi;Bae, Chun-Sik;Kang, Seong-Soo
    • Journal of Veterinary Clinics
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    • v.33 no.1
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    • pp.30-33
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    • 2016
  • It is difficult to detect and diagnose a mass at the tongue base, particularly if the mass is located under normal appearing mucosa. Computed tomography (CT), particularly post-contrast images, is useful to evaluate a tongue mass and adjacent structures including cervical lymph nodes. Nevertheless, a definitive diagnosis of a mass is obtained by histopathological examination. In this case, we describe a mass arising from the tongue body to the root in a dog. The patient was referred with ptyalism and an eating disorder. An oral examination was performed, and decreased tongue motility was observed. CT scanning was performed, and a lingual mass was detected. A lingual mass sample was collected by biopsy at the postmortem 2 months later. A histopathological examination was performed, and the lingual mass was diagnosed as squamous cell carcinoma.

Myocardial degeneration in Russian rat snake (Elaphe schrenckii)

  • Tiwari, Shraddha;Aufa, Sulhi;Park, Hyung-Hun;Cho, Ho-Seong;Park, Byung-Yong;Oh, Yeonsu
    • Korean Journal of Veterinary Service
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    • v.41 no.3
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    • pp.217-220
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    • 2018
  • A female Russian rat snake, Elaphe schrenckii, was presented for loss of movement. Physical examination showed the swelling in the area of heart. Radiographic examination revealed cardiomegaly, pericardial effusion, and a soft opacity in the area of swelling. Although pericardiocentesis to remove fluid out from the heart as well as vigorous treatments were given to the Russian rat snake, it died during treatments. Postmortem examination confirmed pericardial effusion of pale yellow, translucent fluid with mild dilation of the right atrium and ventricle. Formalin -fixed paraffin embedded tissue sections were stained with routine H&E and the classical von Kossa's method for histopathological demonstration. Histopathological examination revealed multifocal calcification in myocardium and consists of the displacement of muscular fiber by limy deposits. Congestive heart failure was suspicious for the snake when it was alive. In wild reptiles, muscle degeneration has been reported with nutrition disorders but the present case is the first report of myocardial degeneration in a Russian rat snake and contributes to the rare reports of cardiac disease in snakes.

Acute Progress of Necrotizing Meningoencephalitis in a Dog; Serial Clinical Observation, Magnetic Resonance Imaging, and Histopathological Findings

  • Song, Joong-Hyun;Moon, Jong-Hyun;Kim, Hyun-Soo;Sur, Jung-Hyang;Park, Chul;So, Kyung-Min;Jung, Dong-In
    • Journal of Veterinary Clinics
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    • v.33 no.5
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    • pp.290-294
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    • 2016
  • A 13-month-old intact female poodle dog presented with an acute history of circling and seizure episodes. On the basis of the results of neurologic examination combined with magnetic resonance imaging and cerebrospinal fluid analysis (CSF), meningoencephalitis of unknown etiology (MUE) was suspected. Therapy with mycophenolate mofetil plus prednisolone was initiated, following which the clinical signs showed improvement for only one month before gradually worsening again. Acute progression of the clinical disease was observed, and the patient was euthanized 91 days after initial presentation. This case was definitively diagnosed as necrotizing meningoencephalitis (NME) according to the results of post-mortem histopathological examination. This report describes the clinical findings, serial magnetic resonance imaging (MRI) characteristics, and histopathological changes in a case of acute NME.

Primary Splenic Myxosarcoma in a Dog (개의 비장원발성 점액육종의 발생례)

  • 권오경;홍성혁
    • Journal of Veterinary Clinics
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    • v.17 no.1
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    • pp.252-255
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    • 2000
  • A 13.5kg, 15-year-old male mongrel dog with anemia, anorexia, vomiting and abdominal distension was referred to the Veterinary Medical Center of the Tokyo University. Radiographic and ultrasonographic findings indicated primary splenic tumor. The tumor was located at body of the spleen, which was surgically removed by splenectomy. Histopathological examination of the mass revealed sarcoma type tumor derived from the mesenchymal origin. The patient was in good health after surgery but suddenly showed abdominal distension on the 3 months after. Radiographic findings indicated abdominal neoplasms. The 2nd operation was performed and removed the recurrent mast but suddenly died on the 4th day. Histopathological examination of the tumor was myxosarcoma.

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Malignant Transformation of Craniopharyngioma without Radiation Therapy: Case Report and Review of the Literature

  • Jeong, Tae Seok;Yee, Gi Taek;Kim, Na Rae
    • Journal of Korean Neurosurgical Society
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    • v.60 no.1
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    • pp.108-113
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    • 2017
  • Craniopharyngiomas exhibiting histologic malignancy are extremely rare. Herein, we report the case of a 26-year-old male patient who underwent suprasellar mass excision via an interhemispheric transcallosal approach. Histopathological examination indicated that the craniopharyngioma was of the adamantinomatous subtype. The patient received postoperative medical treatment for endocrine dysfunction and diabetes mellitus without radiation treatment. Two years after the operation, he presented with progressive visual disturbance and altered mentality. Magnetic resonance imaging revealed a huge mass in the suprasellar cistern and third ventricle. He underwent a second operation via the same approach. The histopathological examination showed an adamantinomatous craniopharyngioma with sheets of solid proliferation in a spindled pattern, indicating malignant transformation. Malignant transformation of craniopharyngioma in the absence of radiation therapy has been reported in only five cases, including this one. We present a case of malignant transformation of craniopharyngioma with a brief review of relevant literature.

Unicystic ameloblastoma with diverse mural proliferation - a hybrid lesion

  • Mahadesh, Jyothi;Rayapati, Dilip Kumar;Maligi, Prathima M.;Ramachandra, Prashanth
    • Imaging Science in Dentistry
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    • v.41 no.1
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    • pp.29-33
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    • 2011
  • A 46-year-old man was referred to our hospital for treatment, complaining of swelling on the right mandibular molar region. Radiographic examination revealed a well defined multilocular radiolucent lesion with root resorption of right lower anteriors and molars. Following biopsy, a diagnosis of unicystic ameloblastoma of mural type was made and hemimandibulectomy was performed under general anesthesia. Histopathological examination of the surgical specimen exhibited a unicystic ameloblastoma of luminal, intraluminal, and mural type. Intraluminal proliferation was of plexiform pattern and mural proliferation showed unusual histopathological findings, which revealed follicular, acanthomatous areas coexisted with desmoplastic areas. This mural picture was similar to the so-called 'hybrid lesion of ameloblastoma', whose biological profile is not elicited due to the lack of adequate published reports. Two years follow up till date has not revealed any signs of recurrence.

Fluorescence bioimaging analysis of collagen antibody-induced arthritis in male mice

  • Kang, Jin Seok
    • Journal of Biomedical and Translational Research
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    • v.18 no.4
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    • pp.125-129
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    • 2017
  • The purpose of this study was to investigate the lesions of a mouse collagen antibody-induced arthritis (CAIA) model using fluorescence bioimaging and micro-computed tomography (micro-CT) and to compare it with histopathological examination. Twelve mice were randomly divided into three groups: group 1 (G1) as control, group 2 (G2) as fluorescence probe control and group 3 (G3) as collagen antibody-induced arthritis. The mice of G3 intravenously received anti-type II collagen 5-clone antibody cocktail (2 mg/mouse) on day 0 and intraperitoneally received lipopolysaccharide ($50{\mu}g/mouse$) on day 3. On the while, the mice of G1 and G2 received 0.9% saline in equal volumes at equivalent times. Fluorescence bioimaging and micro-CT analysis were carried out to assess arthritis. Treatment with the collagen antibody cocktail increased the paw thickness of mice compared to those in both the control and probe-treated groups. Fluorescence bioimaging using a near infrared imaging agent showed high intensity in the joints of collagen anti-body-treated mice, whereas those of control mice showed no signal. Micro-CT analysis of the knee joints of collagen antibody-treated mice showed rough and irregular articular appearance, whereas those of control mice showed normal appearance. Histopathological examination of the knee joints of collagen antibody-treated mice revealed destruction of cartilage and bony structure, synovial hyperplasia and infiltration of inflammatory cells. No cartilage destruction or inflammation was observed in control or probe control mice. Taken together, it is concluded that analyses of fluorescent bioimaging made it possible to evaluate CAIA lesions, comparable with those by micro-CT and histopathological examination in mice.