• Title, Summary, Keyword: hemagglutination

Search Result 203, Processing Time 0.031 seconds

Antigenicity Studies of the Aqueous Extract of Fresh Ginseng in Guinea Pigs

  • Lee, Jong-Wha;Rhee, Man-Hee;Park, Jong-Il;Han, Sang-Seop
    • Korean Journal of Pharmacognosy
    • /
    • v.25 no.1
    • /
    • pp.35-40
    • /
    • 1994
  • Aqueous extreact of fresh ginseng (AFG) was examined for the antigenicity in Hartley guinea pigs in comparision with ovalbumin (OVA). When guinea pigs were sensitized with AFG emulsified with complete Freund's adjuvant (CFA), these animals showed negative reactions in active systemic anaphylaxis (ASA), active cutaneous anaphylaxis (ACA) and passive cutaneous anaphylaxis (PCA) tests and passive hemagglutination (PHA) reaction. On the contrary, when guinea pigs were sensitized with OVA emulsified with CFA as positive controls, these animals disclosed positive reactions in ASA, ACA and PCA tests and PHA reaction. From these results, AFG was considered not to possess antigenic properties in guinea pigs. In addition, the dose levels of AFG empolyed in the present experiment were confirmed not to suppress immune reactions.

  • PDF

Effects of Zinc chloride on the Immunobiological Responses of Lipopolysaccharide (리포폴리사카라이드의 면역생물학적 반응에 미치는 염화아연의 영향)

  • 채병숙
    • YAKHAK HOEJI
    • /
    • v.43 no.1
    • /
    • pp.48-54
    • /
    • 1999
  • Effects of zinc chloride (Zn) on the immune responses of lipopolysaccharide (LPS) were studied by using ICR mice. Mice were divided into 4 groups (10 mice/group), and Zn was given to the mice with i.p. injection at 0.3 mg/kg 5 times a week for 14 days, and 1 hr after Zn administration, LPS was given with i.p. injection at 5 mg/kg twice a week. Mice were immunized and challenged with sheep red blood cells (SRBC). Immunobiological responses were evaluated by humoral, cellular and nonspecific immunity. LPS treatment significantly increased the relative weights of spleen and thymus, hemagglutination titer (HA) and proliferation of splenocytes compared with those in controls, but significantly decreased the body weight gain. Zn treatment significantly increased proliferation of splenocytes and circulating leukocytes compared with those in controls. Combination of Zn and LPS significantly decreased the body weight gain and proliferation of splenocytes compared with those in controls. Combination of Zn and LPS significantly decreased HA and proliferation of splenocytes than in LPS alone. These findings indicate that zinc lowered the humoral immune responses of LPS.

  • PDF

A Case of Liver Abscess in A Child (소아에서 발병한 간농양 1예)

  • Oh, Seung-Taek;Choi, Kwang-Hae
    • Yeungnam University Journal of Medicine
    • /
    • v.25 no.1
    • /
    • pp.72-77
    • /
    • 2008
  • Liver abscess in children is rare in developed countries; the incidence is 25 per 100,000 admissions in USA. Common complications are pleural effusion, empyema, pneumonitis, hepatopleural or hepatobronchial fistula, intraperitoneal or intrapericardiac rupture, septic shock, cerebral amebiasis, etc. These complications may lead to death if the management is delayed. However, recent management results in a mortality of less than 15%. We report a case of liver abscess in a child. He manifested with fever and abdominal pain in the right upper quadrant. On computerized tomography scans, multiple cystic lesions were seen in both lobes of the liver and were 5 to 55 mm in size. In laboratory findings, neutrophilic leukocytosis, peripheral eosinophila, elevated values of ESR, C-reactive protein, and elevated serum AST, ALT, ALP and GGT were detected. Furthermore, we determined the organisms in the blood culture and serum. Blood culture was positive for Streptococcus spp., and amebic indirect hemagglutination antibody titer was increased to 1:512.

  • PDF

Lectin Activity and Chemical Characteristics of Escherichia coli, Lactobacillus spp. and Bifidobacterium spp. from Gastrointestinal Mucosa of Growing Pigs

  • Gao, W.;Meng, Q.X.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.17 no.6
    • /
    • pp.863-868
    • /
    • 2004
  • Lectin activities and chemical characteristics of Escherichia coli, Lactobacillus spp. and Bifidobacterium spp. originating from the porcine cecal mucosal layer were studied based on hemagglutination assay (HA) and hemagglutination inhibition assay (HIA). Although all the bacterial strains were able to agglutinate erythrocytes of porcine or rabbit origin, much higher HA titers were consistently observed for Lactobacillus spp. than for E. coli or for Bifidobacterium spp. A remarkable reduction in HA titers occurred by the treatment of E. coli and Lactobacillus spp. with protease or trypsin and of Bifidobacterium spp. with protease, trypsin or periodate. There were no significant effects on the HA titers of the three groups of bacteria after the treatment with lipase. Hemagglutination of E. coli was strongly inhibited by D (+)-mannose and D (+)-galactose; Lactobacillus spp. by $\alpha$-L-rhamnose and methyl-$\beta$-galactopyranoside; Bifidobacterium spp. by D (+)-alactose, $\alpha$-L-rhamnose, $\alpha$-L-fucose, L (+)-arabinose, D (+)-mannose, D (-)-fructose at a relatively low concentration (1.43 to 3.75 mg/ml). These results, combined with the enhanced HA activities of the three bacterial strains by modification of rabbit erythrocytes with neuraminidase and abolished HA activity of E. coli after treatment with $\beta$-galactosidase, indicate that it might be the glycoproteinous substances surrounding the surface of the bacterial cells that are responsible for the adhesions of these microorganisms by recognizing the specific receptors on the red blood cell.

The Distribution of Hemagglutination Inhibition Antibodies for Japanese Encephalitis Virus Against the Koreans 1976 (한국인의 일본뇌염바이러스에 대한 면역체(HI) 보유율)

  • Lee, Yun-Tai;Lee, Chong-Hoon
    • The Journal of the Korean Society for Microbiology
    • /
    • v.12 no.1
    • /
    • pp.51-56
    • /
    • 1977
  • 1. The total 1,204 of human sera from January to December, 1976 of the out-patients in St. Mary's Hospital were tested one by one. The total 57.6% of the 1,204 cases were confirmed as a hemagglutination inhibition antibodies, and 58.18% out of 574 male cases and 57.14% of 630 female cases were confirmed to have antibodies, respectively. 2. The monthly distribution of hemagglutination inhibition antibody was shown to be 91.2% out of 34 cases in October, 1976 but other months it was low. 3. In the group whose age was from 51 to 60, it was 64.6% and this shows that the group was markedly higher than others.

  • PDF

Changes of Serum antibody Levels by Indirect Hemagglutination Test after Treatment in Clonorchiasis (IHA(Indirect hemagglutination test)를 이용한 간흡충증(肝吸虫症)의 면역혈청학적(免疫血淸學的) 진단(診斷)에 관한 연구(硏究) -ELISA법과의 비교(比較) 및 치료(治療) 전후(前後) 의 혈청항체가(血淸抗體價) 변동(變動)에 관하여-)

  • Joo, Kyoung-Hwan;Kim, Jong-Seong;Chung, Myung-Sook;Rim, Han-Jong
    • Journal of agricultural medicine and community health
    • /
    • v.12 no.1
    • /
    • pp.117-123
    • /
    • 1987
  • Detection of IgG antibody in clonorchiasis has been accomplished through various serodiagnostic procedure including complement fixation test, gel diffusion test, indirect fluorescent antibody test, indirect hemagglutination test etc. In this report enzyme immunoassay (ELISA) and indirect hemagglutination test (IHA) were used to determine IgG serum antibody levels before and after therapy with praziquantel. Briefly, sera from 62 cases of confirmed human clonorchiasis were examined before and after treatment with praziquantel. Among 62 cases treated 25 cases were categorized as completely cured groups by formalin-ether and careful examination of 4 cellophane thick smered slides at 18 months after treatment. The sera of 25 cases of cured groups were examined again by ELISA and IHA, and com-pared to the previous data. The results obtained were as follows; 1) Sensitivity of IHA test was 83.6% when cut-off titer of 1:8 was applied. No sera obtained from 10 normal healthy control showed positive reaction. 2) Twenty cases (80.0%) out of 25 cured one showed negative results by IHA at 18 months after treatment. 3) Although 5 cases showed positive titer even 18 months after treatment 3 cases of them showed decreased antibody titer. However 2 cases did not show any response. 4) Even though almost all cases showed de- creased ELISA value, only 11 cases (44.0%) out of 25 patients showed negative results by ELISA at 18 months after treatment. In conclusion, it is suggested that, while IgG ELISA for detecting long persisting antibody was more sensitive than IHA, IHA results more conclusively indicated effective treatment in clonorchiasis by negative conversion than did the results of ELISA.

  • PDF

Comparison of Biochemical Characterization of Korean and Chinese Mung Bean Lectin (한국산 녹두와 중국산 녹두에 있어서 Lectin의 생화학적 특성 비교)

  • Roh, Kwang Soo
    • Journal of Life Science
    • /
    • v.24 no.6
    • /
    • pp.603-611
    • /
    • 2014
  • The lectins were separated from Korean and Chinese mung bean seeds finally via chromatography using Sephadex G-100 and their biochemical features were studied and compared. They showed no hemagglutination with human red blood cells regardless of trypsin treatment and showed hemagglutination with only trypsin treated rabbit red blood cells. The molecular weights of two lectins were identified as 54 kDa and 28 kDa by SDS-PAGE. It was found that while the optimal reaction temperature of the lectin from Korean mung bean was $60^{\circ}C$, that of the lectin from Chinese mung bean seeds was $50^{\circ}C$. It was found also that the most thermal stable temperature of the seed lectin from Korean mung bean seeds was $50^{\circ}C$ and the lectin from Chinese mung bean was $40-50^{\circ}C$. The lectin from Korean mung bean seeds showed the highest activity at pH 3.2 and the lectin from Chinese mung bean showed the highest activity at pH 6.2. It was identified that when treating a denaturant, thiourea and guanidine-HCl resulted in no hemagglutination, so they induced denaturalization. It was identified also that there was no hemagglutination with urea, so it did not induced denaturalization. They showed no septicity to 6 types of carbohydrates including D-glucose. In addition, the lectins from the two mung bean seed had specificity to metal ions.

Effect of Serum Antibody on Infectious Bronchitis Virus and Its Pathogenicity to SPF Chicks (닭 전염성 기관지염 바이러스에 대한 항체 및 병원성에 관한 연구)

  • Kim Soon-Jae
    • Journal of the korean veterinary medical association
    • /
    • v.25 no.5
    • /
    • pp.282-291
    • /
    • 1989
  • Incidence of infectious bronchitis virus(IBV) infection in vaccinated breeder chickens was investigated by hemagglutination inhibition(HI)test for IBV using Mass 41 antigen. In the breeder chickens with the reduced egg production. chalky deposit. wrinkled

  • PDF

Studies on Passive Hemagglutination Test and Skin Test for Toxoplasmosis in Swine (돼지 Toxoplasmosis 의 간접 적혈구응집반응과 피내반응에 관한 연구)

  • Suh, Myung Deuk;Jang, Du Hwan
    • Korean Journal of Veterinary Research
    • /
    • v.12 no.1
    • /
    • pp.51-58
    • /
    • 1972
  • Hemagglutinating antigen of Toxoplasma gondii was prepared and purified by the method of a slight modification of Tsunematsu, and the preparation of the skin test antigen (toxoplasmin) was made by means of acetone-ether treatment described by Nobute et al. With these antigens the passive hemagglutionation and skin tests were performed for the diagnosis of swine toxoplasmosis by using artificially infected pigs. The results obtained were summarized as follows: 1. The hemagglutinating antibody and the skin test antibody were demonstrated one and three weeks after infection, respectively. And these antibodies were maintained over nine weeks after infection. 2. The antigenicity of hemagglutinating antigen was stable when it was kept in frozen state, while was unstable in a liquid state. 3. Freeze-dried skin test antigen (toxoplasmin) was stable for two months or more if it was kept at $5^{\circ}C$ and room temperature, but in the liquid or reconstituted state it was unstable. 4. Freeze-dried skin test antigen could be preserved without loss of antigenicity for more than two months. 5. Passive hemagglutination test could be applied effectively at the early phase of the disease process and skin test at later phase, mainly for epidemiological survey. However, by combiniation of these methods, the more accurate results could be obtained.

  • PDF