• Title, Summary, Keyword: hemagglutination

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Antigenicity Studies of the Aqueous Extract of Red Ginseng in Guinea Pigs

  • Lee, Jong-Wha;Rhee, Man-Hee;Park, Ki-Hyun
    • Archives of Pharmacal Research
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    • v.17 no.3
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    • pp.154-160
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    • 1994
  • The antigenicity of the aqueous extract of red ginseng (ARG) was evaluated using the following assay procedures : 1. active systemic anaphylaxis (ASA) in guinea pigs, 2 active cutaneous anaphylaxis (ACA) in guinea pigs, 3 passive cutaneous anaphylaxis (PCA) in guinea pigs, 2.active cutaneous anaphylaxis (ACA) in guinea pigs, 3. passive cutanepous anaphylaxis (PCA) in guina pigs with serum for guina pigs sensitized with ARG and 4. passive hemagglutination (PHA) with serum from guinea pigs sensitized with ARG. 1. ASA : No anaphylaxis reaction was observed in any of the sensitized guinea pigs by elictitation with ARG. 2. ACA : No skin reaction was observed in sensitized guinea pigs after intrademal injection of ARG. 3. PCA in guinea pigs : PCA titer of sera from all the sensitized animals was less than 10 in eliciation with ARG. 4. PHA reaction : When eythrocytes coated with challenge antigen were added to sensitized sera, the hemagglutination titer was less than 1. These results suggest that ARG has no antigenicity under the conditions used. And the dose levels of ARG employed in the present experiment were confirmed not to suppress immune reactions.

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Evaluation for Serological Patterns and Fecal Viral Shedding by Hemagglutination Inhibition Test and Real-time PCR in Korean CPV-2 isolates

  • Moon, Hyeong-Sun;Lee, Joon-Seok;Nam, So-Jeong;Yoon, Soon-Seek;Kang, Moon-Il;Jeoung, Seok-Yong;Kim, Doo;Hyun, Chang-Baig
    • Journal of Veterinary Clinics
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    • v.25 no.6
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    • pp.435-439
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    • 2008
  • We evaluated the patterns of serology and fecal viral shedding for any differences by hemagglutination inhibition (HI) and real-time PCR on Korean CPV-2 isolates (CPV-2a-I, CPV-2a-V and CPV-2b). We successfully detected fecal viral shedding from samples extracted 2-3 d.p.i., regardless of the onset of clinical signs. In addition, the pattern of viral shedding differed depending on the CPV-2 isolates used for inoculation. We also observed differences in the serological pattern that was also depended on the CPV-2 isolates inoculated. The onset and amount of fecal viral shedding were not correlated with the level of antibody titers in this study. Our study is a valuable resource for understanding the different pathobiology of the CPV-2 isolates and the correlation between the patterns of serum antibody titer and fecal viral shedding.

Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency

  • Park, Mi-Ja;Joh, Seong-Joon;Choi, Kang-Seuk;Kim, Aeran;Seo, Min-Goo;Song, Jae-Young;Yun, Seon-Jong
    • Korean Journal of Veterinary Research
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    • v.56 no.3
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    • pp.189-192
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    • 2016
  • The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The $r^2$ values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 $log_{10}$ and HI titer of 5 $log_2$ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.

STUDIES ON SALMONELLA PULLORUM ANTIGENS BY MEANS OF INDIRECT HEMAGGLUTINATION TEST (혈구응집반응(血球凝集反應)에 의(依)한 Salmonella pullorum 항원(抗原)에 대(對)한 연구(硏究))

  • Kang, Byong Kyu
    • Korean Journal of Veterinary Research
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    • v.6 no.1
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    • pp.10-17
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    • 1966
  • The antigenecity of somatic substances of S. pullorum standard strain and variant strain extracted byheat treatment, acid treatment and their modification, ammonium sulfate saturation (60 per cent), trypsin digestion was tested by indirest hemagglutination test and precipitation test and following results were optained. 1. Teatment at $100^{\circ}C$ for an hour of the bacteria could extract the antigen of S. pullorum standard strain and variant strain which was demonstrable by hemagglutination reaction with the human a group and chicken red blood cell. 2. Trypsin digestion was more enhanced its antigenecity in acid extracted antigen of S. pullorum variant strain compare with the S. pullorum standard strain. 3. The extracted antigenic substances of S. pullorum standard strain existed chiefly in the elicited fraction of precipitate at the treatment of ammonium sulfate saturation and after trypsin digestion, its antigenecity was demonstrated by hemagglutination. 4. At the treatment of ammonium sulfate treatment, did not occur the precipicate in acid extracted antigens of S. pullorum variant strain, however, the heal extracted antigen, positive reactions were obtained in both of the precipitate and supernatant fraction of the S. pullorum variant strain by hemagglutination reaction. After trypsin digestion, these fraction also exhibited positive reactions. 5. Precipitation test also tested dub could not detect in any soft of the antigens.

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Effects of Houttuyniae Herba on Immune Responses and Histological Findings in Mice Bearing Pneumonitis (어성초추출물(魚腥草抽出物)이 폐렴유발(肺炎誘發) 생쥐의 면역반응(免疫反應) 및 조직변화(組織變化)에 미치는 영향(影響))

  • Song, Ho-Joon;Shin, Min-Kyo
    • Korean Journal of Pharmacognosy
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    • v.18 no.4
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    • pp.216-232
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    • 1987
  • In order to investigate the preventive and therapeutic effects of Herba Houttuyniae, experimental studies on the immune response and histological findings were undertaken. The results of these studies were summarized as follows; Delayed type hypersensitivity and rosette formation rate were significantly increased compared with control group. Hemagglutination, hemolysin titer and NK cell activity were variable, not significantly against the control group. When observed on lung tissue recovery; experimental groups were shown to be significantly recovered as compared with the control group. From these results, it is suggested that Houttuyniae Herba promotes immune response and reduces the recovery time of pneumonitis and lung abscess from the histological viewpoint.

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Studies on Histological Changes of Bursa of Fabricius in Chicken Treated with Thyroxine II. Effect of Thyroxine on Antibody Production (갑상선(甲狀腺) 호르몬이 닭의 Fabricius낭(囊)에 미치는 조직학적변화(組織學的變化)에 관(關)한 연구(硏究) II. 갑상선(甲狀腺) 호르몬이 항체산생(抗體産生)에 미치는 영향(影響))

  • Kim, Soon Bok;Lee, Cha Soo
    • Korean Journal of Veterinary Research
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    • v.20 no.2
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    • pp.99-104
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    • 1980
  • The effects of thyroxine (TX) or propylthiouracil (PPT) administration on the antibody forming activity agains t sheep red blood cell (SRBC) and Newcastle disease virus (NDV) were studied by using of hemagglutination and hemagglutination-inhibition techniques. Antibody titers to both SRBC and NDV increased significantly in the TX-treated group, whereas decreased in the PPT-treated group, compared with control. When TX was administered after antigen inoculatioon, antibody forming activity was significantly enhanced, compared with the TX administration before antigen inoculation.

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H.P.L. Value in Abnormal Pregnancy by Hemagglutination-Inhibition Reaction (비정상임산부(非正常妊産婦)의 혈청중(血淸中) H.P.L.의 혈구응집조지반응(球凝集阻止反應)에 의한 측정(測定)에 관(關)한 연구(硏究))

  • Kim, Dong-Jin;Shin, Myon-Woo
    • Clinical and Experimental Reproductive Medicine
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    • v.4 no.1
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    • pp.27-32
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    • 1977
  • Serum levels of human placental lactogen were measured by hemagglutination inhibition reaction in 26 normal pregnant state and in patients with 16 toxemia and 6 F.D.I.U. beyond their thirtieth week of gestation to evaluate their clinical usefulness. It was realized that HPL-HAIR Test Kit was easy to use and produced reliable results. The general conclusion were as follows: 1) HPL value was $6{\sim}8$ug/ml in normal pregnancy. 2) The levels in mild toxemia were similar in the normal state. 3) The levels in severe toxemia were similar or slightly lower than in the normal and mild toxemia. 4) The levels in F.D.I.U. were lower than in the normal state.

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Molecular Cloning and Expression of Rice Lectin in Escherichia coli (벼 렉틴 유전자의 클로닝 및 대장균에서의 발현)

  • 홍성관;전상훈;김하형;공광훈
    • YAKHAK HOEJI
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    • v.46 no.4
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    • pp.270-275
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    • 2002
  • The lectin gene from rice was amplified by the polymerase chain reaction. The amplified DNA was inserted into the expression vector pET26b and expressed it as a fusion protein with polyhistidine sequences in Escherichia coli. The recombinant protein was produced by induction with 0.4 mM isopropyl-$\beta$-D-thiogalactopyranoside at 37$^{\circ}C$ and purified by an immobilized metal affinity chromatography. The recombinant protein was found to have lectin activity by the hemagglutination inhibition assay. The hemagglutination activity of the recombinant protein was optimal at pH 4.0-7.0 and was dependent on $Ca^{2+}$ and Mn$^{2+}$.2+/.

Immunopotentiating Effect of Polysaccharide Produced from a Mutant of Acetobacter pasteurianus (II) (Acetobacter pasteurianus 변이주가 생산하는 다당류의 면역효과(II))

  • 김동석;정연봉조덕제류병호
    • KSBB Journal
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    • v.7 no.1
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    • pp.45-50
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    • 1992
  • Footpad swelling having the relationship with arthus-reaction of antibody-mediated hypersensitivity and delayed type hypersensitivity was recovered to the almost normal level. The nethemoglobin induced by aniline showed no significant deviation. PFC/spleen cell and PFC/106 spleen cell were increased slightly, but not in case of RFC. Hemagglutination value was increased slightly, but hemolytic value was not changed significantly. Mice in the administration of the polysaccharide does not show any significant stress factor in the cage for mice admlrustered plasma corticosterone. Key words: antibody-mediated hypersensitivity, delayed type hypersensitivity, methemoglobin, Hemagglutination value, hemolytic value, plasma corticosterone.

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