• Title, Summary, Keyword: gastritis

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Effects of the CYP2C19 Genetic Polymorphism on Gastritis, Peptic Ulcer Disease, Peptic Ulcer Bleeding and Gastric Cancer

  • Jainan, Wannapa;Vilaichone, Ratha-Korn
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.24
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    • pp.10957-10960
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    • 2015
  • Background: The CYP2C19 genotype has been found to be an important factor for peptic ulcer healing and H. pylori eradication, influencing the efficacy of proton pump inhibitors (PPIs) and the pathogenesis of gastric cancer. The aim of this study was to investigate clinical correlations of the CYP2C19 genotype in patients with gastritis, peptic ulcer disease (PUD), peptic ulcer bleeding (PUB) and gastric cancer in Thailand. Materials and Methods: Clinical information, endoscopic findings and H. pylori infection status of patients were assessed between May 2012 and November 2014 in Thammasat University Hospital, Thailand. Upper GI endoscopy was performed for all patients. Five milliliters of blood were collected for H. pylori serological diagnosis and CYP2C19 study. CYP2C19 genotypes were determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (RFLP) and classified as rapid metabolizer (RM), intermediate metabolizer (IM) or poor metabolizer (PM). Results: A total of 202 patients were enrolled including 114 with gastritis, 36 with PUD, 50 with PUB and 2 with gastric cancer. Prevalence of CYP2C19 genotype was 82/202 (40.6%) in RM, 99/202 (49%) in IM and 21/202 (10.4%) in PM. Overall H. pylori infection was 138/202 patients (68.3%). H. pylori infection was demonstrated in 72% in RM genotype, 69.7% in IM genotype and 47.6% in PM genotype. Both gastric cancer patients had the IM genotype. In PUB patients, the prevalence of genotype RM (56%) was highest followed by IM (32%) and PM(12%). Furthermore, the prevalence of genotype RM in PUB was significantly greater than gastritis patients (56% vs 36%: p=0.016; OR=2.3, 95%CI=1.1-4.7). Conclusions: CYP2C19 genotype IM was the most common genotype whereas genotype RM was the most common in PUB patients. All gastric cancer patients had genotype IM. The CYP2C19 genotype RM might be play role in development of PUD and PUB. Further study in different population is necessary to verify clinical usefulness of CYP2C19 genotyping in development of these upper GI diseases.

Virulence Genes of Helicobacter pylori in Gastritis, Peptic Ulcer and Gastric Cancer in Laos

  • Vannarath, Sengdao;Vilaichone, Ratha-Korn;Rasachak, Bouachanh;Mairiang, Pisaln;Yamaoka, Yoshio;Shiota, Seiji;Binh, Tran Thanh;Mahachai, Varocha
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.20
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    • pp.9027-9031
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    • 2014
  • Background: Helicobacter pylori (H. pylori) infection is an established cause of peptic ulcers and gastric cancer. The aim of this study was to identify H. pylori genotypes and to examine their associations with geographical regions and gastritis, peptic ulcers and gastric cancer in Laos. Materials and Methods: A total of 329 Lao dyspeptic patients who underwent gastroscopy at Mahosot Hospital, Vientiane, Laos during December 2010 - March 2012 were enrolled. Two biopsy specimens (one each from the antrum and corpus) were obtained for CLO testing and only CLO test-positive gastric tissue were used to extract DNA. PCR and sequencing were identified for variants of the cagA and vacA genotypes. Results: Some 119 Laos patients (36.2%) were found to be infected with H. pylori including 83 with gastritis, 13 with gastric ulcers (GU), 20 with duodenal ulcers (DU) and 3 with gastric cancer. cagA was detected in 99.2%. East-Asian-type cagA (62%) and vacA s1c (64.7%) were predominant genotypes in Laos. vacA s1c-m1b was significantly higher in GU than gastritis (53.8% vs. 24.1%; P-value=0.04) whereas vacA s1a-m2 was significantly higher in DU than gastritis (40.0% vs. 16.9%; P-value=0.03). East-Asian-type cagA and vacA s1c were significantly higher in highland than lowland Lao (100% vs. 55.8%; P-value=0.001 and 88.2% vs. 61.5%, P-value=0.03 respectively). Conclusions: H. pylori is a common infection in Laos, as in other countries in Southeast Asia. The cagA gene was demonstrated in nearly all Laos patients, cagA and vacA genotypes being possible important factors in explaining H. pylori infection and disease outcomes in Laos.

Protective Effect of Liriodendrin Isolated from Kalopanax pictus against Gastric Injury

  • Sohn, Yoon Ah;Hwang, Seon A;Lee, Sun Yi;Hwang, In Young;Kim, Sun Whoe;Kim, So Yeon;Moon, Aree;Lee, Yong Soo;Kim, Young Ho;Kang, Keum Jee;Jeong, Choon Sik
    • Biomolecules & Therapeutics
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    • v.23 no.1
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    • pp.53-59
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    • 2015
  • In this study, we investigated the inhibitory activities on gastritis and gastric ulcer using liriodendrin which is a constituent isolated from Kalopanax pictus. To elucidate its abilities to prevent gastric injury, we measured the quantity of prostaglandin $E_2$ ($PGE_2$) as the protective factor, and we assessed inhibition of activities related to excessive gastric acid be notorious for aggressive factor and inhibition of Helicobacter pylori (H. pylori) colonization known as a cause of chronic gastritis, gastric ulcer, and gastric cancer. Liriodendrin exhibited higher $PGE_2$ level than rebamipide used as a positive control group at the dose of $500{\mu}M$. It was also exhibited acid-neutralizing capacity (10.3%) and $H^+/K^+$-ATPase inhibition of 42.6% ($500{\mu}M$). In pylorus-ligated rats, liriodendrin showed lower volume of gastric juice ($4.38{\pm}2.14ml$), slightly higher pH ($1.53{\pm}0.41$), and smaller total acid output ($0.47{\pm}0.3mEq/4hrs$) than the control group. Furthermore liriodendrin inhibited colonization of H. pylori effectively. In vivo test, liriodendrin significantly inhibited both of HCl/EtOH-induced gastritis (46.9 %) and indomethacin-induced gastric ulcer (46.1%). From these results, we suggest that liriodendrin could be utilized for the treatment and/or protection of gastritis and gastric ulcer.

Improving Effects with Upper Gastrointestinal Diseases Treated with Brown Rice and Germinated Brown Rice (현미와 발아현미의 상부 위장관 보호 효능)

  • Lee, AhReum;Kim, SungHyun;Kwon, OJun;Roh, Seong-Soo
    • The Korea Journal of Herbology
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    • v.31 no.5
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    • pp.85-92
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    • 2016
  • Objectives: This study is experimental comparison of brown rice (BR) and germinated brown rice (GBR) on upper gastrointestinal diseases animal models.Methods: The ICR mice were divided randomly into four groups of six animals each (Normal mice, gastritis mice, gastritis mice treated with BR, gastritis mice treated with 48h GBR). Gastritis was induced by administration of 0.5 mL 150 mM HCl-60% ethanol. Six-week-old male Sprague-Dawley (SD) rats were randomly divided into 7 groups after 1 week adaptation. (Normal rat, reflux esophagitis (RE) rat, RE rat treated with BR, RE rat treated with 24,30,36,48h GBR). Reflux esophagitis was induced by ligation with a 2-0 silk thread both the pylorus and the transitional junction between the forestomach and the corpus in SD rats.Results: HCl/ethanol-induced gastric mucosal injury mice were ameliorated mucosal damage upon histological evaluation by treatment of 48h GBR than BR. Optical changes such as hyperemia and multiple erosions were observed in the rats with RE and damage to the normal rats was not apparent. The oral administration of GBR significantly diminished against gross mucosal damage in a germination time-dependent manner. Also, the administration of GBR suppressed the biomarker of oxidative stress, reactive oxygen species (ROS) and produces peroxynitrite (ONOO-) in serum. However, the administration of GBR could not affect to the pH level secreted from stomach when compared with Control group.Conclusions: These findings suggest that GBR could have improving effects on upper gastrointestinal diseases in a germination time-dependent manner.

$Luteolin7-O-{\beta}D-glucuronopyranoside$ has the protective effect on gastritis and esophagitis in rats

  • Bae, Ki-Lyong;Yim, Sung-Hyuk;Min, Young-Sil;Park, Joon-Hong;Choi, Hee-Jung;Ham, In-Hye;Hwang, Wan-Kyunn;Sohn, Uy-Dong
    • Proceedings of the PSK Conference
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    • pp.265.2-265
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    • 2002
  • It is well known that flavonoids are the inhibitory effects on inflammations. This study was designed to determine the anti-inflammatory effects of luteolin-7-O-${\beta}$D-glucuronopyranoside (LGC). newly synthesized flavonoids. which was extracted from Salix gilgiana leaves. We investigated the protective action of LGC on reflux esophagitis and gastritis in rats. Esophagitis and gastritis were induced by surgical procedures and the exposure to indomethacin (50 mg/kg), respectively. LGC was injected intraduodenally immediately after the surgical procedures and the exposure to indomethacin (omitted)

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Gastritis in a Dog Infected with Helicobacter heilmannii (Helicobacter heilmannii 균 감염이 확인된 개에서의 위장염 발생 증례)

  • 황철용;윤화영;한홍율
    • Journal of Veterinary Clinics
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    • v.19 no.3
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    • pp.375-378
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    • 2002
  • A 3-year-old intact female korean pungsan dog showing chronic intermittent vomiting and anorexia was admitted to The Veterinary Medical leaching Hospital of Seoul National University. The patient had been received antiemetics, antacids or proton pump inhibitor for these symptoms but had not been recovered yet. All results of physical examination, complete blood count, serum chemical analysis, fecal test and radiography were normal. Gastric endoscopic examination was also performed and gastritis with hemorrhage on near antrum region was detected. In PCR assay of bioptic gastric tissues for detecting Helicobacter species infection, Helicobacter heilmannii infection was confirmed. After receiving triple therapy with amoxicillin, metronidazole and omeprazole for 21 days, all symptoms were recovered and had not been occurred again. Helicobacter heilmannii also were not detected.

The Effects of Hyang-Sa-Yuk-Gun-Ja-Tang and Oh-Pe-San on The Gastric Mucosal Repair (위장관(胃腸管) 점막층(粘膜層)의 회복(恢復)에 향사육군자탕(香砂六君子湯)과 오패산(烏貝散)이 미치는 영향(影響))

  • Chang, Hen-Yel;Kang, Yoon-Ho
    • The Journal of Dong Guk Oriental Medicine
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    • v.2 no.1
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    • pp.127-137
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    • 1993
  • In order to investigate the clinical efficiency based on Oriental Medical References of Hyang-Sa-Yuk-Gun-Ja-Tang and Oh-Pe-San, histological studies have been done by using the experimental animal. The following results were obtained. 1. The Hyang-Sa-Yuk-Gun-Ja-Tang showed a significant promoting effect of repair on the epithelial layer from the basement membrane of duodenal ulcer in rats. 2. The Oh-Pe-San showed efficiencies about repair of the necrosis in epithelial layer on the acute gastritis in rats. According to above results, it is concluded that Oh-Pe-San and Hyang-Sa-Yuk-Gun-Ja-Tang were recognized to be effective on the acute gastritis and the duodenal ulcer in rats, and this study may suggest a clinical guidance to the prescriptions for the treatment of gastritis end gastric ulcer.

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Anti-oxidant Effects of Portulaca oleracea L. on HCl-ethanol Induced Gastritis in Rats (염산-에탄올에 의해 유발된 흰쥐 위염에 대한 마치현의 항산화 작용)

  • Kim, Chae-Hyun
    • The Korea Journal of Herbology
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    • v.24 no.1
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    • pp.35-40
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    • 2009
  • Objectives : The purpose of this study is to evaluate whether or not a pretreatment with Portulaca oleracea has an antioxidant effect in HCl-ethanol induced gastric mucosal damage. Methods : We elucidated the level of reactive oxygen species (ROS), lipid peroxidation, and two important constituents of antioxidant defense such as superoxide dismutase (SOD), glutathione (GSH) in these effects. Results : The oral administration of crude extract from P. oleracea attenuated the gastritic lesion area, submucosal edema and hemorrhage, and mucosal necrosis induced by HCl-ethanol. The MDA levels of control group were higher than those in the rats given the P. oleracea pretreatment. While the GSH levels of control were decreased, the GSH activity on the gastric mucosal layer maintain normal level in rats given the Portulaca oleracea pretreatment before HCl-ethanol induced gastritis significantly increased. However, the SOD activites were not altered by P. oleracea. Conclusions : The administration of Portulaca oleracea have a protective antioxidant effect against the gastric lesion induced by HCl-ethanol and may therefore be a promising drug for gastritis and gastric ulcer.

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Suppressive Actions of Astragali Radix (AR) Ethanol Extract and Isolated Astragaloside I on HCl/ethanol-Induced Gastric Lesions

  • Jeong, Jeong-Suk;Lee, Je-Hyuk;Lee, Sang-Hyup;Kang, Sam-Sik;Jeong, Choon-Sik
    • Biomolecules & Therapeutics
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    • v.17 no.1
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    • pp.62-69
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    • 2009
  • Roots of Astragalus membranaceus (A. membranaceus) (Astragali Radix, AR) has been used as a herbal medicine for gastrointestinal disorders in China, Korea, Japan, and other Asia countries. In this study we investigated the effects of the AR 70% ethanol extract and compounds isolated from AR on gastritis in rats and growth of human gastric cancer cells. AR 70% ethanol extract showed the potent acid-neutralizing capacities and partly cytotoxicity of Helicobacter pylori and human AGS gastric cancers cell. Astragaloside I and daucosterol, which were isolated from AR, significantly inhibited HCl/ethanol-induced gastric lesions. In addition, daucosterol increased the mucus content to almost the same as the positive control. The results of this study suggest that astragaloside I and daucosterol may be good candidates for the development of new drugs or neutraceuticals which can be used for the treatment or prevention of gastritis.

Protective Effect of Litsea japonica Fruit Flesh Extract on Stress-induced Gastritis in Rats (흰쥐에서 스트레스로 유발된 위염에 대한 까마귀쪽나무열매 추출물의 보호 효과)

  • Park, In-Jae;Park, Sung-Hwan;Yun, Ji-Hyun;Choi, Goo-Hee;Kim, Hyun-Jung;Seo, Yun-Hee;Cho, Ju-Hyun
    • Journal of Food Hygiene and Safety
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    • v.32 no.6
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    • pp.536-541
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    • 2017
  • The object of this study was to investigate the inhibitory effects of Litsea japonica fruit flesh extract (LJF-HE) on gastritis of an stress-induced SD rat model. Rats were randomly divided into six groups: Normal (normal group), Control (stress-induced gastritis), Ranitidine (stress-induced gastritis and ranitidine 50 mg/kg), LJF-HE-L (stress-induced gastritis pretreated with L. japonica fruit flesh extract at 30 mg/kg), LJF-HE-M (stress-induced gastritis pretreated with L. japonica fruit flesh extract at 60 mg/kg), LJF-HE-H (stress-induced gastritis pretreated with L. japonica fruit flesh extract at 120 mg/kg). In groups treated with LJF-HE, gastric mucosal damage and pepsin activity were reduced. Additionally, there were decreases in the expression of cholecystokinin 2 receptor (CCK-2r) in the gastric lesions. The plasma levels of IL-$1{\beta}$ slightly but significantly decreased in LJF-HE treated groups compared to control. The plasma level of PGE2 was also significantly increased in LJF-HE treated groups. These results suggest that LJF-HE has the ability to reduce of the severity stress-induced gastritis.