• Title, Summary, Keyword: gallotannin

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A Study on the Effect of Gallotannin Treatment of Ceramide-containing Fibers on Atopic Skin Diseases (세라마이드 함유 섬유의 복합갈로탄닌 처리에 의한 아토피성 피부질환 완화작용에 대한 연구)

  • Kim, Taekyeong;Cho, Nayoung;Ma, Heejung;Yang, Gwang Wung;Rho, Yong Hwan
    • Textile Coloration and Finishing
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    • v.25 no.4
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    • pp.271-278
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    • 2013
  • In order to investigate the effect of gallotannin treatment to ceramide-containing fabrics on atopic skin diseases, the agglomeration of standard protein BSA and the deactivation of model enzyme were examined. The gallotannin treated on ceramide-containing fabrics precipitated the standard protein, BSA, and therefore deactivated the model enzyme by 70% at 6% treatment concentration. Wash durability should be improved after around 5 cycles of washing. Clinical test of the gallotannin-treated fabrics was carried out on mice for two test items, transepidermal water loss assay and severity score of diseased skin of mice. The results showed significant level of improvement of atopic skin diseases compared with the negative controled.

A Gallotannin from Cercidiphyllum japonicum Leaves

  • Lee, Tae-Seong;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.5
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    • pp.558-565
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    • 2015
  • Katsura tree (Cercidiphyllum japonicum Sieb. Et Zucc) leaves were collected, air-dried and extracted with 70% aqueous acetone, then concentrated and sequentially fractionated using n-hexane, methylene chloride ($CH_2Cl_2$), ethylacetate (EtOAc), and $H_2O$. The EtOAc fraction was chromatographed on a Sephadex LH-20 column with various aqueous MeOH eluting solvents and finally treated with acetone-$H_2O$ (7:3, v/v) to isolate a gallotannin. According to the NMR analysis, including HSQC and HMBC, and with the comparison of authentic literature data, the isolate was elucidated as 6-m-digalloyl-1,2,3,4-tetra-O-galloyl ${\beta}$-D-(+)-glucose, one of hydrolyzable tannins and one of gallotannins. The compound was only gallotannin which was firstly isolated from the extracts of Katsura tree leaves, and has not been reported before in domestic tree sources.

A Study on Natural Dyeing ( I ) - Dyeing of Cotton Fabric with Amur Cork Tree - (천연염색에 관한 연구 (1) - 황벽에 의한 면 염색 -)

  • 김혜인;엄성일;박수민
    • Textile Coloration and Finishing
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    • v.13 no.1
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    • pp.1-8
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    • 2001
  • Amur cork tree is natural basic and yellow dye largely used for dyeing on cotton. The most effective solvent for extract of berberine from amur cork tree was methanol. The dyeabilities of the colorant on cotton and the fastness of cotton fabrics dyed with amur cork tree extract were investigated. And according to the concentration, temperature and time of pre-treatment, K/S values, wash fastness and Hand Value of cotton fabrics dyed with amur cork tree extract were investigated. As a results, K/S values of dyed cotton fabrics were increased by Pre-treatment with chinese gallotannin and the optimum condition of chinese gallotannin treatment was 15%owf concentration at $60^\circ{C}$, 30min. The wash fastness was enhanced to 3.5 grades. There was no degradations of Hand Value of cotton fabrics treated with chinese gallotannin.

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A Study on Natural Dyeing (4) - Dyeing of Silk Fabric with Amur cork tree Extract - (천연염색에 관한 연구(4) -황벽에 의한 견 염색-)

  • 김혜인;엄성일;박수민
    • Textile Coloration and Finishing
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    • v.13 no.5
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    • pp.320-328
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    • 2001
  • Amur cork tree contains yellow natural basic dye largely used for dyeing on silk. Dyeabilities and fastnesses of the colorant on silk fabrics were investigated , According to dye concentration, pH, temperature, time of pre- and post- treatment of mordant, K/S values and fastnesses of the silk fabrics were examined. As a results, K/S values of dyed silk fabrics were increased by pre- and post-treatment with chinese gallotannin. The optimum rendition of chinese gallotannin pre-treatment was 0.15% concentration, pH 4, $70^\circ{C}$, and 45min, and that of post-treatment was 0.05% concentration,$60^\circ{C}$, and 30min. The wash fastness and the light fastness were enhanced to 4 grades and 2~3 grades.

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Anti-oxidative Phenolic Compounds from Sophorae Fructus

  • Kim, Hyun-Jung;Kim, Min-Kee;Shim, Jae-Gul;Yeom, Seung-Hwan;Kwon, Suk-Hyung;Lee, Min-Won
    • Natural Product Sciences
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    • v.10 no.6
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    • pp.330-334
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    • 2004
  • Four isoflavonoids and three flavonoids, and a gallotannin were isolated from the fruits of Sophora japonica (Leguminosae). Their structures were identified as genistein (1), sophoricoside (2), genistein-4'-O--L-rhamnopyranoside (3), $genistein-4'-O-{\alpha}-L-rhamnopyranosly-(1{\rightarrow}2)-{\beta}-D-glucopyranoside$ (4), $kaempferol-3-O-{\alpha}-D-sophoroside$ (5), $kaempferol-3-O-{\beta}-D-glucopyranosyl-(1{\rightarrow}2)-{\alpha}-L-rhamnopyranosly-(1{\rightarrow}6)-{\beta}-D-glucopyranoside$ (6), rutin (7) and gallic acid $4-O-{\beta}-D-(6'-O-galloyl)-glucopyranoside$ (8) by chemical and spectroscopic analysis and comparisons with previously reported spectral data. Compounds 3 and 8 were isolated for the first time from this plant. Anti-oxidative activity was evaluated for the isolated compounds. 8 exhibited potent anti-oxidative activity against the radical scavenging ability of DPPH with the $IC_{50}$ value of $17.1\;{\mu}g/ml$.

Gallotannin regulates apoptosis and COX-2 expression via Akt and p38kinase pathway in human lung cancer cell line, A549

  • Yu, Seon-Mi;Gweon, Eun-Jeong;Chung, Ki-Wha;Kim, Kwang-Hoon;Cho, Hong-Sik;Kim, Song-Ja
    • Animal cells and systems
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    • v.16 no.5
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    • pp.366-375
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    • 2012
  • Gallotannin (GT) is derived from plant poly phenol and is associated with biological actions in a wide range of cells. In this study, we evaluated the effect of GTon apoptosis and cyclooxygenase-2 (COX-2) expression and attempted to shed light on the mechanism of action in A549 human lung carcinoma cells. We found that GT dramatically induced apoptosis as demonstrated by expression of p53 and active caspase-3 via western blot analysis and fragmented DNA as detected by DNA fragmentation and DAPI staining. We also observed that GT significantly causes COX-2 expression in a dose-dependent manner determined by western blot analysis. Phosphorylation of Akt and p38 was considerably increased by GT in A549 human lung carcinoma cells. Inhibition of Akt and p38kinase with LY294002 or SB203580 suppressed GT-induced apoptosis and COX-2 expression. Furthermore, we have shown that prevention of COX-2 with NS398 or indomethacin does not any effects on apoptosis induced by GT. Taken together, our present results suggest that GT regulates apoptosis and COX-2 expression through Akt and p38kinase pathway in A549, human lung carcinoma cells.

Anti-Allergic Effect of 1,2,3,4,6-Penta-O-Galloyl-β-D-Glucose on RBL-2H3 Cells (RBL-2H3 세포에서 1,2,3,4,6-Penta-O-Galloyl-β-D-Glucose의 항알레르기 효과)

  • Kim, Yoon Hee;Choi, Ye Rang;Kim, Ji Young;Kwak, Sang Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.4
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    • pp.613-618
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    • 2016
  • 1,2,3,4,6-Penta-O-galloyl-${\beta}$-D-glucose (PGG) is a gallotannin isolated from various plants such as Galla Rhois. In a previous study, it was reported that PGG has anti-allergic effects by inhibiting interleukin (IL)-4 signaling in B cells. However, the effect of PGG on basophilic cells remains unclear. Therefore, the aim of this study was to investigate the inhibitory effect of PGG on mitogen and calcium ionophore-induced allergic responses. PGG had no effect on proliferation and cytotoxicity of RBL-2H3 cells. PGG significantly suppressed cell degranulation (histamine and ${\beta}-hexosaminidase$) as well as inflammatory cytokine production such as IL-4 and tumor necrosis factor-${\alpha}$. The underlying mechanism of PGG on these anti-allergic actions was correlated with inhibition on translocation of nuclear factor-${\kappa}B$ from the cytosol to nucleus. These data suggest that PGG is a potentially effective functional compound for prevention of allergic diseases.

A Study on Natural Dyeing (3) - Change of physical structure and Chinese gallotannin treatment on silk fabric - (천연염색에 관한 연구 (3) -견에 대한 Chinese gallotannin 처리 및 구조변화-)

  • 김혜인;엄성일;박수민
    • Textile Coloration and Finishing
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    • v.13 no.5
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    • pp.289-297
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    • 2001
  • As the basic study to investigate the mechanism of improvement in the dyeing fastness of natural dyes on silk fabrics the optical behavior of tannin solution, the optimal condition of tannin treatment and the changes of the physical structure of silk fabrics were studied in the treatment of tannin. Results obtained were as follows ; 1 The K/S values of tannin-treated silk fabrics were not changed by tannin content on silk fabrics but yellowing of tannin in treatment condition. 2. As the concentration of tannin was increased in its treating condition, the tannin content and K/S vague of tannin-treated silk fabrics were increased. 3. Because tannin gave rise to yellowing by hydrolysis and oxidation at high temperature, the higher tannin-treating temperature was, the lower the tannin content and K/S values of silk fabrics 4. The tannin content of silk fabric was higher in acidic treating condition and the highest K/S value was showed at pH 5. 5. As the tannin content of silk fabric was increased, the value of $\xi$ -potential of it became negative. 6. As the tannin content of silk fabric was increased, the crystallinity and crystallite size of it were decreased. This meant that the fraction of amorphous region in the fibers increased. And the degree of orientation of it was decreased with the tannin treatment.

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Antioxidative Activities and Quantitative Determination of Gallotannins from Barks of Acer ginnala Maxim (신나무 수피로부터 Gallotannin 화합물의 항산화 활성 및 함량분석)

  • Choi, Sun Eun;Park, Kwan-Hee;Oh, Myoeng-Hwan;Jang, Jun-Hye;Jin, Hye-Young;Kim, Sung-Sik;Lee, Min-Won
    • Korean Journal of Pharmacognosy
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    • v.41 no.3
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    • pp.174-179
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    • 2010
  • Activity guided isolation of 80% acetone extract from the barks of Acer ginnala Maxim. yielded five gallotannins [6-galloyl-1,5-anhydroglucitol (ginnalin B) (1), acertannin (3,6-digalloyl-1,5-anhydroglucitol) (2), methyl gallate (3), acertannin (2,6-digalloyl-1,5-anhydroglucitol) (4) and gallic acid (5)]. All of these isolated compounds from Acer ginnala(1-5) were firstly isolated from Acer ginnala Maxim. And contents of compounds from barks of Acer ginnala (Comp. 1: 0.73$\pm$0.002%, Comp. 2: 0.48$\pm$0.001%, Comp. 3: 0.66$\pm$0.002%, Comp. 4: 1.05$\pm$0.002% and Comp. 5: 0.29$\pm$0.001%) were evaluated by HPLC analysis. And, in order to evaluate anti-oxidative activities on Comp. 1-5 isolated from Acer ginnala, DPPH radical scavenging activity was measured in vitro. All of these isolated compounds from Acer ginnala exhibited potent DPPH radical scavenging activities.