• Title, Summary, Keyword: fucoxanthin

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Concentration of Fucoxanthin from Ecklonia cava Using Supercritical Carbon Dioxide (초임계 이산화탄소를 이용하여 감태로부터 Fucoxanthin 농축)

  • Lee, Bo-Mi;Kim, Chul-Jin;Kim, Chong-Tai;Seo, Jung-Ju;Kim, In-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.10
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    • pp.1452-1456
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    • 2009
  • Fucoxanthin has been concentrated efficiently using supercritical carbon dioxide. First, fucoxanthin was extracted from Ecklonia cava using solvents. Three solvents, such as medium chain fatty acid (MCFA), medium chain fatty acid ethyl ester, and fatty acid from coconut oil were tested, and MCFA was selected as a suitable solvent to extract fucoxanthin from Ecklonia cava. The concentration was conducted at various pressures and temperatures. In order to concentrate fucoxanthin from extracts, MCFA was eliminated from the extract using supercritical carbon dioxide, and fucoxanthin was concentrated successfully in residue. Optimal conditions for concentrating the fucoxanthin from fucoxanthin extract were $40^{\circ}C$ at 9.7 MPa, $45^{\circ}C$ at 11.0 MPa, and $50^{\circ}C$ at 12.4 MPa, respectively. The density of carbon dioxide affected the concentration of fucoxanthin from fucoxanthin extract. In this optimal conditions, the density for concentrating the fucoxanthin was 600 g/L.

Characteristic and Extraction of Fucoxanthin Pigment in Undaria pinnatifida (미역에 함유된 Fucoxanthin 색소의 추출 및 특성)

  • 김선재;김현주;문지숙;김정목;강성국;정순택
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.5
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    • pp.847-851
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    • 2004
  • The contents of fucoxanthin in Undaria pinnatifida blade, stem and sporophyll were 87.6 mg/100 g, 62.4 mg/100 g and 127.7 mg/100 g, respectively. The fucoxanthin was analysed by using solvent fractionation, silica gel adsorption chromatography and HPLC techniques. Absorption spectrum of carotenoids extracted from Undaria pinnatifida was similar to the fucoxanthin carotenoids in sea mustard. The cleavage products formed by autoxidation of fucoxanthin were evaluated in order to elucidate possible oxidation products of fucoxanthin in liposomal suspension. Fucoxanthin solubilized at 50 $\mu$M in liposomal suspension formed five oxidized products. These results suggest that fucoxanthin might be cleaved to a series of cleavage products under the oxidative condition in liposomal suspension.

Extraction of Fucoxanthin from Undaria pinnatifida and Stability of Fucoxanthin (미역으로부터 후코산틴 추출 및 후코산틴 안정성)

  • Shin, Soo Cheol;Ahn, Myeong Won;Lee, Jung Shik;Kim, Young Suk;Park, Kwon Pil
    • Korean Chemical Engineering Research
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    • v.51 no.1
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    • pp.42-46
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    • 2013
  • Extraction process of fucoxanthin from Undaria pinnatifida and stability of fucoxanthin was studied. In this study, to utilized extracted components as a functional food material, ethanol was used as a solvent. The maximum concentration of fucoxanthin was obtained when 80% ethanol solvent was used. The extraction yield of fucoxanthin increased as extraction temperature raised to $50^{\circ}C$. Water washing of raw material for 5 minutes reduced the salt content about 94%. From the experiment that fucoxanthin content reduced by 30% for 1 day storage at $70^{\circ}C$, it was demonstrated that fucoxanthin was thermal-unstable above $70^{\circ}C$. And experimental result of light- stability showed that fucoxanthin was very unstable with light.

Fucoxanthin Protects Cultured Human Keratinocytes against Oxidative Stress by Blocking Free Radicals and Inhibiting Apoptosis

  • Zheng, Jian;Piao, Mei Jing;Keum, Young Sam;Kim, Hye Sun;Hyun, Jin Won
    • Biomolecules & Therapeutics
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    • v.21 no.4
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    • pp.270-276
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    • 2013
  • Fucoxanthin is an important carotenoid derived from edible brown seaweeds and is used in indigenous herbal medicines. The aim of the present study was to examine the cytoprotective effects of fucoxanthin against hydrogen peroxide-induced cell damage. Fucoxanthin decreased the level of intracellular reactive oxygen species, as assessed by fluorescence spectrometry performed after staining cultured human HaCaT keratinocytes with 2',7'-dichlorodihydrofluorescein diacetate. In addition, electron spin resonance spectrometry showed that fucoxanthin scavenged hydroxyl radical generated by the Fenton reaction in a cell-free system. Fucoxanthin also inhibited comet tail formation and phospho-histone H2A.X expression, suggesting that it prevents hydrogen peroxide-induced cellular DNA damage. Furthermore, the compound reduced the number of apoptotic bodies stained with Hoechst 33342, indicating that it protected keratinocytes against hydrogen peroxide-induced apoptotic cell death. Finally, fucoxanthin prevented the loss of mitochondrial membrane potential. These protective actions were accompanied by the down-regulation of apoptosis-promoting mediators (i.e., B-cell lymphoma-2-associated ${\times}$ protein, caspase-9, and caspase-3) and the up-regulation of an apoptosis inhibitor (B-cell lymphoma-2). Taken together, the results of this study suggest that fucoxanthin defends keratinocytes against oxidative damage by scavenging ROS and inhibiting apoptosis.

Isolation and Characterization of Indigenous Diatom, Odontella sp. BS-003 as Potential Fucoxanthin and Omega-3 Fatty Acid Producer (잠재적 푸코잔틴 및 오메가-3 지방산 생산자로서 토착 규조류 오돈텔라의 분리 및 배양 특성)

  • Heo, Jina;Cho, Dae-Hyun;Kim, Urim;Kim, Hee-Sik
    • Journal of Marine Bioscience and Biotechnology
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    • v.10 no.1
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    • pp.26-33
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    • 2018
  • Fucoxanthin has been reported as bioactive compounds exhibiting strong antioxidant, anticancer and anti-inflammatory activities. Owing to its a wide range of applications and potentials, commercial production of fucoxanthin from algae has been attracted many attentions. Although, most of seaweeds and diatoms contain fucoxanthin as major carotenoid contents, low productivity of fucoxanthin still hinder the industrial application. Here, we newly isolated and identified indigenous marine diatom Odontella sp. BS-003 as a resource of fucoxanthin production. The characterization, optimization and production of the fucoxanthin, along with other bioactive compound omega-3 fatty acid from odontella sp. BS-003 were analyzed in this study, and the results represented optimal culture condition (two-fold silicate containing F/2 medium) significantly enhanced the algal biomass productivity. The maximum biomass (1.83 g/L), fucoxanthin (3.88 mg/g), along with omega-3 fatty acid (10 %, w/w) were obtained from the 10 L of photobioreactor. Based on the results, it is speculated that the microalga Odontella sp. BS-003 can be a promising natural resource for the production of bioactive compounds.

Isolation and Characterization of Indigenous Marine Diatom Achnanthidium sp. BS-001 Producing a high Content of Omega-3 Fatty Acid and Fucoxanthin Production (오메가-3 지방산 및 푸코잔틴 고함량 토착 규조류 아크난티디움 균주의 분리 및 특성)

  • Kim, Urim;Cho, Dae-Hyun;Heo, Jina;Kim, Hee-Sik
    • Journal of Marine Bioscience and Biotechnology
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    • v.11 no.1
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    • pp.14-22
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    • 2019
  • Omega-3 fatty acids and carotenoids, which are known as representative high-value substances derived from microalgae, are being studied from various diatoms. Most of the diatoms contain fucoxanthin and omega-3 fatty acid. Fucoxanthin produced by diatom has been reported as bioactive compounds exhibiting strong antioxidant, anticancer and anti-inflammatory activities. However, the low growth rate and fucoxanthin content of diatoms are one of the big obstacles to the industrial application. In this study, indigenous marine diatom Achnanthidium sp. BS-001 was isolated for a candidate of fucoxanthin producer. Light intensity and temperature for the culture of Achnanthidium sp. BS-001 were optimized on PhotoBiobox. Optimization of silicate concentration for increasing BS-001 biomass productivity was confirmed in F/2 medium with various concentration of sodium silicate. As a result, condition of light intensity, temperature, and silicate concentration for optimal cultivation were $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, $18^{\circ}C$ and 0.106 mM, respectively. Maximum biomass productivity reaches to $154.3mg{\cdot}L^{-1}{\cdot}day^{-1}$, and then the content of omega-3 fatty acids and fucoxanthin were $19.4mg{\cdot}g^{-1}$, $9.05mg{\cdot}g^{-1}$, respectively. These results indicate that Achnanthidium sp. BS-001 has the potential to be used as a source of omega-3 fatty acids and fucoxanthin.

Bioactive Carotenoid, Fucoxanthin as Chemotaxonomic Marker and Antioxidative Agent from the Marine Bacillariophycean Microalga Hantzschia marina

  • Choi, Jin-Seok;Lee, Won-Kap;Cho, Yong-Jin;Kim, Dong-Soo;Kim, Ae-Ra;Chung, Hae-Young;Jung, Jee-H.;Im, Kwang-Sik;Choi, Won-Chul;Choi, Hong-Dae;Son, Byeng-Wha
    • Natural Product Sciences
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    • v.6 no.3
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    • pp.122-125
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    • 2000
  • Allenic and epoxy carotenoid, fucoxanthin (1) was isolated from the marine bacillariophycean microalga Hantzschia marina and the structure was assigned on the basis of comprehensive spectroscopic analyses. Fucoxanthin was detected only from diatom among three families (green algae, diatom and blue-green algae) of the marine microalgae tested. Fucoxanthin showed free radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and peroxynitrite $(ONOO^-)$ with $IC_{50}$ values of $32\;{\mu}M\;and\;60\;{\mu}M$, respectively.

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Suppressive Effects of Fucoxanthin on Degranulation in IgE-antigen complex-stimulated RBL-2H3 Cells

  • NamKoong, Seung;Joo, Hae-Mi;Jang, Seon-A;Kim, Ye-Jin;Kim, Tae-Seong;Sohn, Eun-Hwa
    • Korean Journal of Plant Resources
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    • v.25 no.3
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    • pp.339-345
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    • 2012
  • The marine carotenoid fucoxanthin can be found in marine brown seaweeds, macroalgae, diatoms, and microalgae, and has remarkable biological properties. Numerous studies have shown that fucoxanthin has considerable potential and promising applications in human health, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. We here investigated the mechanisms by anti-allergic activity of fucoxanthin fraction from Eisenia bicyclis in immunoglobulin E-antigen complex (IgE/DNP-BSA)-stimulated RBL-2H3 mast cells. This study we found that the fucoxanthin inhibits the release of ${\beta}$-hexosaminidase and suppressed not only transcriptional activation of NF-${\kappa}B$, but also phosphorylation of ERK and JNK in IgE/DNP-BSA-treated RBL-2H3 cells. Fucoxanthin may be useful for preventing allergic diseases, including asthma and atopic dermatitis.

Antioxidant effects of fucoxanthin rich powder in rats fed with high fat diet

  • Ha, Ae Wha;Na, Se Jung;Kim, Woo Kyoung
    • Nutrition Research and Practice
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    • v.7 no.6
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    • pp.475-480
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    • 2013
  • The purpose of this study was to determine the antioxidant effect of fucoxanthin. After rats were fed a normal fat diet (NF), high fat diet (HF), and high fat with 0.2% fucoxanthin diet (HF + Fxn) for 4 weeks, the markers of oxidative stress and antioxidant capacity like lipid peroxidation, plasma total antioxidant capacity (TAC), and activities of antioxidant enzymes (catalase, superoxide dismutase (SOD), and gluthathione peroxidase (GSH-Px)) were determined. mRNA expression of transcription factor, nuclear erythroid factor like 2 (Nrf2), and its target genes such as NAD(P)H quinone oxidoreductase1 (NQO1) and heme oxygenase-1 (HO-1) were also determined. Mean weight gain in the HF + Fxn group was lower, without statistical significance, and the total food intake in the HF + Fxn group was lower than that in the HF group (P < 0.05). The activity of GSH-Px (P < 0.05) in plasma was significantly higher in the HF + Fxn group than those in the HF group (P < 0.05). In the liver, the activities of catalase (P < 0.05) and GSH-Px (P < 0.05) in the HF + Fxn group were significantly higher than those in the HF group. Plasma TAC level was significantly higher in the HF + Fxn group than that in the HF group (P < 0.05). Lipid peroxidation in plasma tended to be lower without statistical significance. Fucoxanthin supplements were shown to have higher mRNA expression of Nrf2 and NQO1 than those in the high fat diet only group (P < 0.05). In conclusion, supplementation of fucoxanthin improved the antioxidant capacity, depleted by high fat diet, by activating the Nrf2 pathway and its downstream target gene NQO1. Therefore, supplementation of fucoxanthin, especially for those who consume high fat in their diet, may benefit from reduced risk of oxidative stress.

Change of fucoxanthin and total antioxidant capacities of Saccharina japonica during the drying process (다시마의 건조 과정 중에 발생하는 후코잔틴(fucoxanthin)과 총항산화능의 변화)

  • Baek, Su Hyeon;Lee, Hye Ju;Lee, Chae Hyeon;Nam, Taek-Jeong;Lee, Sang Gil
    • Korean Journal of Food Science and Technology
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    • v.51 no.6
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    • pp.524-530
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    • 2019
  • Kelp (Saccharina japonica) contains various bioactive compounds, including vitamins, minerals (especially iodine and potassium), alginic acid, fucoxanthin, and various antioxidants. Kelp is mainly used as a dried product. The purpose of this study was to investigate the stabilities of antioxidant capacities and fucoxanthin of kelp by different conventional drying conditions including hot air drying at 70℃ and natural drying methods. Fucoxanthin, total phenolic contents, and total antioxidant capacity by ABTS, DPPH, and FRAP assays were significantly decreased after 5 to 10 h of hot air drying at 70℃. The assay values were also significantly decreased by 50% after 2 days of drying due to ultraviolet exposure. The findings demonstrate that heat and ultraviolet exposure during drying of kelp could affect the degradation of bioactive compounds, especially fucoxanthin and polyphenols. Optimal conditions should be considered during kelp drying and storage.