• Title, Summary, Keyword: free radical

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Study on the effect of Sagunjatang(SGJT) and Samultang(SMT) extract on the Wave of Human body and Active Oxygen (사군자탕(四君子湯) 및 사물탕(四物湯)이 인체파동(人體波動)과 활성산소(活性酸素)에 미치는 영향(影響)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Cho, Ki-Yong;Yoo, Dong-Youl
    • Journal of Haehwa Medicine
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    • v.9 no.1
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    • pp.305-317
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    • 2000
  • To prove the effect of Sagunjatang(SGJT) and Samultang(SMT) extract on the wave of Human Body and Active Oxygen experimentally, QRS & Free Radical of extract and precipitate of SGJT and SMT was measured. The results were summarized as follows ; 1. SGJT extract and precipitate, as a result of measuring QRS and Free Radical, had a considerable effect on the function of immunity & pituitary gland. 2. BaekBokRung of SGJT, as a result of measuring QRS and Free Radical, had a considerable effect on the function of heart & spleen. 3. BaekChul and Gamcho of SGJT, as a result of measuring QRS and Free Radical, had a considerable effect on the function of stomach & duodenum. 4. YinSam of SGJT, as a result of measuring QRS and Free Radical, had a considerable effect on the function of immunity & spleen. 5. SMT extract and precipitate, as a result of measuring QRS and Free Radical, had a considerable effect on the function of immunity & uterus. 6. SukJiHwang of SMT, as a result of measuring QRS and Free Radical, had a considerable effect on the function of immunity & liver. 7. DangGui of SMT, as a result of measuring QRS and Free Radical, had a considerable effect on the function of heart & spleen. 8. ChunGung of SMT, as a result of measuring QRS and Free Radical, had a considerable effect on the function of spleen & pituitary gland. 9. BaekJakYak of SMT, as a result of measuring QRS and Free Radical, had a considerable effect on the function of stomach & duodenum. 10. SMT extract had an effect on the function of female Urogenital system, SGJT extract had an effect on the function of male Urogenital system. These results suggested that SGJT and SMT extract might be usefully applied for suppresing of Active Oxygen formation, preventing the aging, curing all the disease resulted from Active Oxygen.

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Changes of Free Radical Concentration with Irradiation Dose and Storage Time in Gamma-Irradiated Dried Vegetables (감마선 조사된 건조채소류에서 조사선량과 저장기간에 따른 Free Radical 농도의 변화)

  • 남혜선;양재승
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.5
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    • pp.854-857
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    • 2001
  • Electron spin resonance (ESR) spectroscopy was used to detect free radicals in irradiated dried vegetables. Dried carrot, mushroom and green onion were irradiated with doses of 0, 1, 3, 5 and 7 kGy at room temperature using a Co-60 irradiator. Free radicals were detected in irradiated dried carrot, green onion and mushroom, while those were not detected in non-irradiated samples. Since concentration of free radicals linearly increased with applied doses (1~7 kGy), highly positive correlation coefficients ($R^2$=0.9747~0.9919) were obtained between irradiation doses and free radical concentrations right after irradiation. Although concentration of free radicals slowly decreased with storage time, the characteristic ESR signals of free radicals slowly decreased with storage time, the characteristic ESR signals of free radical in irradiated samples were observed even after 3 months of storage at room temperature.

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An analysis of the measurements of the obesity using the code of Quantum Resonance Imprinting System(QRIS) with infusion and using the Free Radical Measurement(Ultra-weak Chemiluminescence) with soak in water regarding medicines which would be mostly used for the treatment of the obesity (비만 범용 처방(處方)에 대한 탕전후(湯煎後) 경락 기능 진단기(QRIS)의 비만 연관 Code측정 및 수침후(水浸後) Free Radical 측정기를 이용한 측정에 대한 고찰)

  • Kim, Jae-Joong;Lim, Hyung-Ho;Sun, Yu-Jin;Lee, Dong-Hoon
    • The Journal of Korea CHUNA Manual Medicine
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    • v.1 no.1
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    • pp.83-90
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    • 2000
  • Oryeong-San, Pangpungtongseong-San, Rangkyeoksanwha-Tang, Sipeemikwanjoong -Tang and Taeumjoui-Tang are not only examined using the code which are related to overweight in the QRIS but are also investigated the level of Free Radical using the Free Radical Measurement after steeping those 5 prescriptions in water. The results are as follows: 1. We indicated in the study of QRIS that 5 kinds of medicines which used most frequently In the treatment of the obesity appeared to affect the Immune system, spleen, kidneys, pancreas, the fatigue toxicity, TSH, and the metabolic disability but did not influence high on the contents of overweight and those of fatty cell, as well. in addition, there were no significant differences between the prescriptions as regards testosterone and progesterone. 2. In the Free Radical Measurement, Rangkyeoksanwha-Tang evaluated significantly high level of Free Radical, whereas others appear to have the similar level of Free Radical. These findings suggest that the treatment of the obesity affects particular body parts with respect to the control of overweight, although those medicines are not related directly to the areas(such as fatty cell Code), it is possible that they influence on the cure for the obesity. Furthermore, they indicate that with soaking prescription, Free Radical is not produced as much as we expected.

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Acetycholinesterase Inhibiting Effect and Free Radical Scavenging Effect of Soybean(Glycine max) and Yak-Kong(Rhynchosia nolubilis) (대두와 약콩의 Acetylcholinesterase 활성 억제효과와 항산화 효과)

  • 강순아;한진아
    • Journal of the East Asian Society of Dietary Life
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    • v.14 no.1
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    • pp.64-69
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    • 2004
  • The deficiency of the neurotransmitter acetylcholine(ACh) is responsible for the initiation of Alzheimer's disease. In addition, there is a simple evidence that oxidative stress significantly increases in persons with Parkinson's disease compared to age-matched healthy persons. Therefore, the objective of the study was to investigate the neurodegeneration inhibitory effect of soybean(Glycine Max) and Yak-Kong(Rhynchosia Nolubilis) by measuring the degree of inhibiting Acetylcholinesterase (AChE) catabolizing the ACh and the free radical scavenger effect in vitro. AChE was measured by the minor modified Ellman assay. Free radical scavenging activity was measured using l-diphenyl-2-picrylhydrazyl (DPPH). First, the MeOH extracts of Soybean and Yak-Kong showed the AChE inhibiting activity of 62.0$\pm$2.43% and 65.0$\pm$3.29% at the 5 mg/$m\ell$ concentration. The 50% inhibitory concentration ($IC_{50}$/) of AChE was 1.96 and 1.31 mg/$m\ell$ in the MeOH extracts of soybean and Yak-Kong. Second, the MeOH extracts of soybean and Yak-Kong showed the free radical scavenger activity of 23.1$\pm$4.26% and 80.7$\pm$4.61% at the 5 mg/$m\ell$. IC50 of free radical scavenger activity in Soybean and Yak-Kong was 13.00 and 1.41 mg/$m\ell$ in MeOH extracts and was 5.95 and 2.74 mg/$m\ell$ in hot-water extracts, respectively. In this study, the extracts of Soybean and Yak-Kong showed powerful effects in the AChE inhibition and free radical scavenging. The extracts of Soybean and Yak-Kong were expected to prevent the many neurodegenerative diseases.

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Detection of Gamma-Irradiated Wheat Flour and Glutinous Rice Flour by Measuring Free Radical (Free radical의 측정을 통한 감마선 조사된 밀가루와 찹쌀가루의 검지)

  • Lee, Eun-Jeong;Yang, Jae-Seung
    • Korean Journal of Food Science and Technology
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    • v.34 no.2
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    • pp.170-173
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    • 2002
  • Electron spin resonance (ESR) spectroscopy was used to detect free radicals in irradiated wheat flour and glutinous rice flour. They were irradiated with doses of 0, 0.1, 0.5, 1 and 2 kGy at room temperature using a Co-60 irradiator. Free radicals detected for irradiated wheat flour and glutinous rice flour were higher than those of unirradiated ones. Since concentration of free radicals linearly increased with applied doses $(0.1{\sim}2\;kGy)$, highly positive correlation coefficients $(R^2=0.9995{\sim}0.9998)$ were obtained between irradiation doses and free radical concentrations right after irradiation. Although concentration of free radicals slowly decreased with storage time, the characteristic ESR signals of free radical in irradiated samples were observed even after 4 weeks of storage at room temperature.

A Case of Maxillary Carcinoma Recontruction with a Fibular Osteocutaneous Free Flap (비골 골-피부 유리 피판을 이용한 상악동 암종 재건 1례)

  • Sun, Dong-Il;Kim, Min-Sik;Kwon, Yong-Jae;Cho, Seung-Ho
    • Korean Journal of Bronchoesophagology
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    • v.6 no.1
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    • pp.118-126
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    • 2000
  • A radical maxillectomy causes a defect of the alveolar bone, gingiva, palate, and orbital floor and causes cosmetical problems and masticatory and phonatory functions. Defect after a radical maxillectomy was reconstructed with skin or dermis graft was introduced, but recently wide resection of the tumor and functional reconstruction with free flap was introduced by several methods. The defect due to radical maxillectomy was reconstructed with scapula, iliac crest, radius. But reconstruction with a fibular osteocutaneous free flap was rarely introduced to defect of radical maxillectomy. The fibular osteocutaneous free flap was firstly introduced by Taylor. The fibular osteocutaneous free flap has several advantages. We experienced the first case of radical maxillectomy and reconstruction with the fibular osteocutaneous free flap, so we reported that case with literatures. The patient has a right maxillary sinus squamous carcinoma (T2N0M0), and performed a radical maxillectomy with right supraomohyoid neck dissection, and reconstruction with fibular osteocutaneous free flap. Donor site morbidity was little, and phonatory and masticatory function were nearly normalized. And cosmetical result was very acceptable.

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Electron Transport Carrier for the Free Radical Shethna Flavoprotein in Azotobacter vinelandii (Azotobacter vinelandii Shethna Flavoprotein 의 Free Radical 생성(生成)을 위(爲)한 전자전달물질(電子傳達物質)-)

  • Chun, Jae-Kun;Tollin, Gordon
    • Applied Biological Chemistry
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    • v.16 no.1
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    • pp.31-40
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    • 1973
  • Azotobacter vinelandii cell extracts and its variety of purified fractions with regard to their ability to form the redox state of the Shethna Flavoprotein (free radical form FPH.) were studied. A fluorescent flavoprotein (protein I) and a brown protein (protein II) were the most active proteins which were isolated in purified form. The free radical formation activity was substantially decreased during the purification and was completely lost upon storage in a week under nitrogen in a frozenstate. The presence of free flavin (FMN) with NADH enhanced the rate of free radical formation. The reaction of FMN and NADH was found to be catalysed by various cell fractions. A possible role of FMN as a substrate for free radical shethna flavoprotein was investigated. Slower reaction rate of $FMNH_2+Flavoprotein\;(FP){\to}FPH+FMN$ than $FMN+NADH{\to}FMNH_2$, accumulation of $FMNH_2$ ocurred which subsquently caused FPH.

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Characterization of the Active Site of Ascorbyl Free Radical Reductase Purified from Pleurotus ostreatus

  • Yu, Seong-Woon;Kang, Sa-Ouk
    • Proceedings of the Korean Biophysical Society Conference
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    • pp.25-25
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    • 1998
  • Ascorbyl free radical reductase was purified from the white rot fungus, Pleurotus ostreatus. The enzyme contained FMN as a prosthetic group, which was reduced by NADH and reoxidized by ascorbyl free radical. Reduction of ascorbyl free radical by the enzyme was observed by EPR spectroscopy.(omitted)

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Antioxidative Effects of Phellinus linteus Extract (상황버섯 추출물의 항산화 효과)

  • Rhyu, Dong-Young;Kim, Min-Suk;Min, Oh-Jin;Kim, Dong-Wook
    • Korean Journal of Plant Resources
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    • v.21 no.1
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    • pp.91-95
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    • 2008
  • Reactive oxygen species(ROS) or free radical-mediated oxidative stress plays an important role in the pathophysiologic process of disease state. This study investigated antioxidative effects of Phellinus linteus extract on the generation of 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical, superoxide anion radical(${O_2}^-$), hydroxyl radical(${\cdot}OH$), nitric oxide(NO), and peroxynitrite($ONOO^-$) radical and free radical-mediated protein oxidation under in vitro assay systems. This results showed that Phellinus linteus extract effectively inhibited the generation of free radicals in the all assay system with dose-dependent manner and also significantly reduced the protein oxidative level. Thus, the present study indicates that Phellinus linteus extract possesses a potent antioxidant activity and plays a beneficial role against free radical-induced oxidative injury.

Polysaccharide Extraction and Comparison of Free Radical Scavenging Activities from Tremella fuciformis and Auricularia auricula Fruit Body (흰목이와 흑목이 버섯의 다당추출 및 유리라디칼 소거활성 비교)

  • Kim, Hyeon-Min;Hur, Won;Lee, Shin-Young
    • Food Engineering Progress
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    • v.15 no.1
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    • pp.6-14
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    • 2011
  • The polysaccharides from fruit body of Auricularia auricula and Tremella fuciformis were extracted using hot water, and partially purified through ethanol precipitation and dialysis. Free radical scavenging activities of the crude and purified polysaccharides were examined and compared each other. Free radical scavenging activities of the partially purified polysaccharides were higher than those of crude polysaccharides. DPPH free radical, ABTS radical and SOD-like activities of partially purified polysaccharide at 1 mg/mL of concentration from A. auricula were 61.7, 9.6 and 38.9%, respectively, while those of T. fuciformis were 9.6, 5.7 and 15.3%, respectively. Results of site and non-site specific hydroxyl radical scavenging activities indicated that the partially purified polysaccharide fractions from A. auricula and T. fuciformis exhibited the hydroxyl radical scavenging effect by hydrogen donating ability and iron ion chelating ability. Also, reducing powers of A. auricula and T. fuciformis were 77.1 and 14.7% of BHT (0.1%) as standard, respectively. It was suggested that antioxidant activities of A. auricula were about 1.4~6.4 times higher than those of T. fuciformis due to different levels of polyphenol content.