• Title, Summary, Keyword: extraction condition

Search Result 1,060, Processing Time 0.042 seconds

Effect of Extraction Condition on the Content of EGCG and Caffeine of Green Tea: Comparison with the Inhibitory Activity on Pancreatic Lipase

  • Lee, Eun Song;Lee, Mi Kyeong
    • Natural Product Sciences
    • /
    • v.19 no.2
    • /
    • pp.166-172
    • /
    • 2013
  • Caffeine and epigallocatechin gallate (EGCG) are major constituents of green tea, the leaves of Camellia sinensis (Theaceae). Although EGCG is well known for diverse beneficial effect, caffeine is sometimes harmful with adverse effects. Therefore, the extraction efficiency was investigated using different extraction method such as extraction solvent, extraction time, extraction method, and repeated extraction. The content of EGCG and caffeine in green tea extract was quantitated by HPLC analysis. The extraction condition exerted difference on the extraction yield. The content of EGCG was also affected by different extraction condition. Especially, the extraction solvent greatly affected the content of EGCG in the extract. However, the content of caffeine was less affected compared to that of EGCG. The inhibitory effect of green tea extract on pancreatic lipase was almost similar regardless of extraction condition. Taken together, optimization of extraction condition will provide best efficacy for further development of green tea as anti-obesity therapeutics.

Optimization of Extraction Condition of Hesperidin in Citrus unshiu Peels using Response Surface Methodology

  • Lee, Jua;Park, Shinyoung;Jeong, Ji Yeon;Jo, Yang Hee;Lee, Mi Kyeong
    • Natural Product Sciences
    • /
    • v.21 no.2
    • /
    • pp.141-145
    • /
    • 2015
  • Hesperidin, which is the most abundant flavonoid of Citrus unshiu (Rutaceae), has been reported to possess diverse activities and widely used as functional foods and cosmetics. For the development of functional products, extraction procedure is indispensable. Extraction conditions affect the composition of extract as well as its biological activity. Therefore, we tried to optimize extraction conditions such as extraction solvent, extraction time and extraction temperature for maximum yield of hesperidin using response surface methodology with threelevel-three-factor Box-Behnken design (BBD). Regression analysis showed a good fit of the experimental data and the optimal condition was obtained as ethanol concentration, 59.0%; temperature $71.5^{\circ}C$ and extraction time, 12.4 h. The hesperidin yield under the optimal condition was found to be $287.8{\mu}g$ per 5 mg extract, which was well matched with the predicted value of 290.5 μg. These results provides optimized extraction condition for hesperidin and might be useful for the development of hesperidin as functional products like health supplements, cosmetics and medicinal products.

Simultaneous Quantitation of Nine Constituents of Fraxinus rhynchophylla using High Performance Liquid Chromatography - Diode Array Detector

  • Ahn, Jong Hoon;Hwang, Bang Yeon;Lee, Mi Kyeong
    • Natural Product Sciences
    • /
    • v.19 no.3
    • /
    • pp.236-241
    • /
    • 2013
  • A high-performance liquid chromatography-diode array detector (HPLC-DAD) method was established for quantitative evaluation of nine constituents of Fraxinus rhynchophylla such as four coumarins, esculin (1), fraxin (2), esculetin (3), fraxetin (4), three lignans, syringaresinol 4,4'-O-${\beta}$-diglucoside (5), pinoresinol 4-O-${\beta}$-glucoside (6), pinoresinol (9), one secoiridoid, oleuropein (7), and one coumarinolignan, cleomiscosin C (8). The preferred chromatographic condition was obtained on Phenomenex Gemini-NX (3 ${\mu}m$, C18 110A, $150{\times}4.60$ mm) and the mobile phase was composed of water and acetonitrile using a gradient elution. The wavelength was set at 220 nm. Extraction condition of these constituents in F. rhynchophylla was also optimized through extraction time, extraction solvent and extraction method using established method. From this study, extraction at $70^{\circ}C$ with the mixture of ethanol and water for more than 12 h was suggested to be good extraction condition for these constituents. Quantitation of nine constituents in different F. rhynchophylla samples was also successfully accomplished with the newly established method.

Optimization of Extraction Condition of Methyl Jasmonate-treated Wild Ginseng Adventitious Root Cultures using Response Surface Methodology

  • Liu, Qing;Jo, Yang Hee;Ahn, Jong Hoon;Kim, Seon Beom;Paek, Kee-Yoeup;Hwang, Bang Yeon;Park, So-Young;Lee, Mi Kyeong
    • Natural Product Sciences
    • /
    • v.24 no.2
    • /
    • pp.103-108
    • /
    • 2018
  • The usage of wild ginseng (Panax ginseng C.A. Meyer) has been limited due to short supply and high price. Therefore, sufficient production as well as efficient extraction of mountain ginseng are required for the development as products. In this study, wild ginseng adventitious root cultures were prepared for efficient production with advantages of fast growth and stable production. Treatment of methyl jasmonate (MJ) to wild ginseng adventitious root cultures increased the extraction yield and antioxidative activity. Further investigation on effect of extraction conditions suggested the importance of ethanol concentration on antioxidative activity and extraction yield of MJ-treated wild ginseng adventitious root cultures. Optimized extraction condition of MJ-treated wild ginseng adventitious root cultures for maximum extraction yield and antioxidative activity was determined using response surface methodology with three-level-three-factor Box-Behnken design (BBD). Extraction of 1 g MJ-treated wild ginseng adventitious root culture with 30 ml of 9% ethanol at $30^{\circ}C$ produced 310.2 mg extract with 71.0% antioxidative activity at $100{\mu}g/ml$. Taken together, MJ-treated wild ginseng adventitious root culture is valuable source for wild ginseng usage and optimized extraction condition can be used for the development of functional products or folk remedies.

The extraction condition of pungent compounds from Zanthoxylum piperitum D.C pericarps by using supercritical fluid extraction (초임계유체 추출에 의한 초피나무 과피 중 신미성분의 추출조건)

  • Lee, Chang-Joo;Kim, Myung-Seok;Shen, Jing-Yu;Kim, Yong-Doo;Shin, Jae-Han
    • Korean Journal of Medicinal Crop Science
    • /
    • v.11 no.1
    • /
    • pp.19-23
    • /
    • 2003
  • The optimum extraction condition of pungent component from Zanthoxylum piperitum D.C pericarps by using supercritical fluid extraction(SFE) was investigated. The optimum condition of SFE was $300kg/cm^2$ of pressure, $60\;^{\circ}C$ of extraction temperature, 80% of $CO_2$ fluid, 20% of modifier(methanol) volume and 20 min of extraction time. The extraction efficiency between the classical solvent extraction method and SFE was studied. About 40% of extraction efficiency was improved when SFE was applied.

Separation of Cr(VI) from Heavy Metal Salts Mixed Solution by using Hollow Fiber Module (실관막모듈에 의한 중금속염 혼합용액으로부터 Cr(VI) 분리)

  • 최대웅
    • Journal of Environmental Health Sciences
    • /
    • v.27 no.3
    • /
    • pp.107-112
    • /
    • 2001
  • This work reports the application of a hollow fiber module(HFM) for Cr(VI) extraction from heavy metal salts mixed solution by using microporous hydrophobic hollow fiber module. In HFM configuration, the organic extraction used for the extraction of Cr(VI) was di-(2-ethyl hexyl) phosphoric acid(D2EHPA) diluted with n-heptane. The study of HFM includes the influence of hydrodynamic and chemical condition, i.e., the flow rate of feed solution, the time of reactive extraction, the concentration of feed solution, and the pH of aqueous phase solutions. Several experiments with synthetic solution of different mixed components system of Cr(VI) solutions established optimum condition to achieve a clean separation of Cr(VI). It was possible to separate Cr(VI) in the presence of metal salts mixed solution, such as Zn(II), Ni(II), Cu(II), and Cd(II) using the HFM technique.

  • PDF

Optimization of Microwave-Assisted Extraction under Atmospheric Pressure Condition for Soluble Ginseng Components (상압조건의 마이크로웨이브 추출공정에서 가용성 인삼성분의 추출조건 최적화)

  • Kwon, Joong-Ho;Kim, Kyung-Eun;Lee, Gee-Dong
    • Korean Journal of Food Science and Technology
    • /
    • v.32 no.1
    • /
    • pp.117-124
    • /
    • 2000
  • Considering the thermal unstableness of ginseng components, microwave-assisted extraction(MAE) was performed under the atmospheric pressure condition. The monitoring of extraction characteristics and the optimization of extraction conditions were made by response surface methodology. The extraction efficiency of soluble ginseng components was high at lower ethanol concentration and at higher microwave power, while crude saponin content was easily extracted at higher ethanol concentration. Estimated conditions for the maximized extraction of soluble components including crude saponin, total phenolics and electron donating ability were $54{\sim}60%$ in ethanol concentration, $41{\sim}90$ W in microwave power, and within 4 min in extraction time. Predicted values at the optimum condition(60% ethanol, 80 W microwave power and 4 min extraction time) were in good agreement with observed values.

  • PDF

Optimized pH condition of protein extraction of Gastrodia elata Blume by alkaline method (알칼리에 의한 천마 단백질 추출의 최적 pH 조건)

  • Jang, Hye-Lim;Yoon, Kyung-Young
    • Korean Journal of Food Preservation
    • /
    • v.22 no.2
    • /
    • pp.256-260
    • /
    • 2015
  • This study investigated the optimum pH condition for the efficient extraction of protein from Gastrodia elata Blume. Five extraction pH values (8, 9, 10, 11, and 12) and three precipitation pH values (2, 4, and 6) were used. The protein content, browning degree, and recovery yield of the protein obtained under each pH condition were determined. Most of the G. elata Blume was made up of carbohydrates, and its protein content was also high. The amount of the extracted protein increased according to the increase in the extraction pH, but did not significantly differ between pH 8 and pH 9. The browning degree of the protein significantly increased as the extraction pH increased. The greatest amount of protein was precipitated at pH 4, the recovery yield of which was also the highest. As a result, it was found that the combination of extraction pH 9 and precipitation pH 4, which resulted in a 38.7% recovery yield and a low browning degree, is the optimum condition for the efficient extraction of protein from G. elata Blume.

Analysis of Total Sugar by Extraction Condition and Material to Develope the Extraction Process of Ginseng Polysaccharide (인삼 다당체 추출 공정 개발을 위한 인삼의 추출 조건 및 원료에 따른 총당 변화)

  • Jang Soon-Ae;Moon Sook-Kyung
    • Korean Journal of Food Preservation
    • /
    • v.12 no.4
    • /
    • pp.367-371
    • /
    • 2005
  • This study analyzed the extraction condition for large-scale extraction process to be used industrially. The total sugar content of 5-year Red ginseng in viewpoint of the ginseng materials was highest by $32\%$ and it of 5-year Keumsan ginseng was $31\%$. Therefore 5-year Keumsan ginseng was used by test sample. The next extraction condition, that is the total sugar content of the internal white among the parts of ginseng, the extraction efficiency under the condition of optimal temperature $80^{\circ}C$ and optimal extract time 6hrs, was highest. And the amount of total sugar extracted from ginseng treated with protease, ${\alpha}-amylase$ and cellulase was increased about $20\%$. Total sugar recovery in methods of alcohol concentration $70\%$ and freezer-dry method was highest.

Development of Granular Tea by Using Astringent Persimmon and Persimmon Leaves (떫은 감과 감잎을 이용한 과립차 개발에 관한 연구)

  • Byun, Gwang-In;Kwon, Yong-Ju;Park, Mi-Lan
    • Culinary science and hospitality research
    • /
    • v.14 no.2
    • /
    • pp.273-285
    • /
    • 2008
  • The purpose of this study was to find appropriate processing condition of astringent persimmon and persimmon leaf granular tea. Under the condition of 4 hour extraction and $50^{\circ}brix$, extraction yield and concentrating of astringent persimmon were the greatest. Under the condition of 2 hour extraction and $7^{\circ}brix$, extraction yield and concentrating of persimmon leaf were the greatest. The optimum condition of granular tea with astringent persimmon and persimmon leaf added was the best in astringent persimmon concentrate of 16% and persimmon leaf concentrate of 4%. It had the best taste after the condition in a brix value of 16.15% and a pH of 5.57. The L-value of chromaticity was -79.13, a-value 2.27, and b-value 2.41. In the sensory properties correlation analysis of astringent persimmon and persimmon leaf granular tea, sweet taste, color acceptability, odor acceptability and taste accept-ability were positively significant correlation with most sensory properties. However, astringent taste was no significant correlation with sensory acceptability. Bitter taste and after taste were negatively significant correlation with most sensory properties.

  • PDF