• Title, Summary, Keyword: extracellular alpha-amylasse

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Properties of an Extracellular Amylase Produced by the Marine Halophilic Bacterium Vibrio alginolyticus (해양 호염성 세균 Vibrio alginolyticus가 생산하는 Extracellular Amylase의 특성)

  • 김영재
    • Microbiology and Biotechnology Letters
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    • v.27 no.3
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    • pp.203-207
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    • 1999
  • V. alginolyticus 138-2, a marine halophilic bacterium, produced an extracellular amylase with a molecular weight of ca. 56,000. The analysis of the digestion products of soluble starch by thin layer chromatography(TLC) revealed that the extracellular amylase of V. alginolyticus 138-2 is a saccharifying-type alpha-amylase. The alpha-amylase activity of the culture supernatant of soluble starch was optimal at pH 6.0 and 45$^{\circ}C$. Ca2+ slightly increased the alpha-amylase activity, whereas Hg2+, An2+, Cu2+, Ni2+, Fe2+, and Mn2+inhibited the enzymatic activity. Alkylating thiol group agent, iodoacetic acid did not affect the alpha-amylase activity, but reduced thiol reagents such as dithiothreitol, cysteine, and beta-mercaptoethanol stimulated theenzymatic activity. On the other hand, even if V. alginolyticus 138-2 is a marine halophilic bacterium, its alpha-amylase activity was significantly inhibited by NaCl.

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